Curso de Formación Específica. Del 17 al 21 de octubre de 2016. Inicio: lunes 17, 9.30 horas. Aula de SECYT (Secretaría de Ciencia y Técnica, Universidad Nacional de Córdoba). Bv. Dr. Juan Filloy s/n, Ciudad Universitaria, Córdoba. Mapa 

Organiza: Departamento de Química Orgánica. Total de horas: 40 horas teóricas.

 

Curso aprobado por Escuela de Posgrado.

 

Cupos limitados: 30 personas.

 

Dirigido a: estudiantes de posgrado (Doctorado y Maestría) que utilicen la espectrometría de masas como herramienta para la obtención de resultados aplicables en sus trabajos de tesis.

 

Objetivo: el curso cubre conceptos preliminares, contenidos avanzados de acuerdo al moderno estado del arte y aplicaciones modernas de la espectrometría de masas para la resolución de problemas científicos en áreas como salud, ambiente, alimentos, fisiología, proteómica y metabolómica. 

 

Plantel docente:

Director: Dr. Daniel Alberto Wunderlin (Profesor Titular – Investigador Principal CONICET, Departamento Química Orgánica; ICYTAC, CONICET-UNC).

Colaboradores: Dra. SANTIAGO, Ana Noemí (Profesora Adjunta, Investigadora Independiente CONICET Departamento Química Orgánica, INFIQC, CONICET, UNC); PhD RODRIGUEZ-MOZAZ, Sara (Investigadora, Departamento ICRA, Girona, España); Dra. AMÉ, María Valeria (Profesora Adjunta, Investigadora Independiente CONICET, Departamento Bioquímica Clínica, CIBICI, CONICET-UNC), Dra. VALDÉS, María Eugenia (Profesora Asistente, Becaria Postdoctoral CONICET, Departamento Bioquímica Clínica; CIBICI, CONICET, UNC), Dra. CERUTTI, Estela Soledad (Profesora Asociada UNSL, Investigadora Independiente CONICET Departamento Química de la Facultad de Química, Bioquímica y Farmacia UNSL, INQUISAL, UNSL, CONICET; Dra. ANDRADE, Andrea Mariela (Ayudante Primera UNRC, Investigadora Asistente CONICET, Departamento Ciencias Naturales, FCEFQyN-UNRC); Dra. MONGE, María Eugenia (Investigadora Adjunta CONICET; Departamento CIBION-CONICET); Dra. MORENO, Silvia Margarita (Profesora Titular Consulta, Investigadora Principal CONICET, Departamento FECyN-UBA, CEQUIBIEM, CONICET, UBA); Dra. BARONI, María Verónica (Profesora Asistente, Investigadora Adjunta CONICET, Departamento Bioquímica Clínica; ICYTAC, CONICET, UNC), Dra. MONFERRÁN, Magdalena Victoria (Profesora Asistente, Investigadora Adjunta CONICET, Departamento Bioquímica Clínica, ICYTAC, CONICET, UNC); Dra. DI PAOLA, Romina Daniela (Profesora Asistente, Profesional Adjunta CONICET, Departamento  Bioquímica Clínica, ICYTAC, CONICET, UNC).

 

Modalidad: cursado presencial, ocho horas diarias, de lunes a viernes. Aprobación: mediante examen final.

 

Aranceles:

-Estudiantes de Doctorado de la FCQ (UNC) y estudiantes de Doctorado de la UNC con cargo docente de la UNC: sin costo (según Res. HCS 02/09).

-Estudiantes de posgrado y becarios, sin cargo docente de la UNC y de otras instituciones nacionales: $1.200

-Egresados/profesionales o extranjeros: $1.200

Alumnos que no pertenezcan a la UNC deben tramitar una beca para cubrir el costo del curso. La beca la otorga el SNEM (Sistema Nacional de Espectrometría de Masas) e incluye el costo de inscripción, traslado y un viático diario para personas que no vivan en Córdoba. Cupo máximo de becas: 15 (quince). La beca no cubre el costo del certificado, el que deberá pagarse aparte en caso de ser necesario.

 

Inscripciones: cerradas

 

Más información:  Dr. Daniel A. Wunderlin. E-mail: dwunder@fcq.unc.edu.ar

 

Organiza: Departamento de Química Orgánica de la FCQ (UNC).

 

PROGRAMA

Principios e Instrumentación actual en Espectrometría de Masas (8 horas)

Dr. Daniel Wunderlin (UNC-ICYTAC) / Dra. Ana Santiago (UNC-INFIQC)

 

Aplicaciones de Espectrometría de masas en estudios ambientales (8 horas)

Dra. Sara Rodriguez-Mozaz (ICRA-Girona-España) – Dra. Ma. Valeria Amé (UNC-CIBICI) – Dra. María Eugenia Valdés (UNC-CIBICI).

 

Aplicaciones de espectrometría de masas en metabolómica  (8 horas)

Estela Soledad Cerutti( INQUISAL UNSL- CONICET) – Dra. Andrea M. Andrade (Lab. Fisiología Vegetal, FCEFQyN, UNRC).

 

Aplicaciones de espectrometría de masas en estudios clínicos y Proteómica (8 horas)

Dra. María Eugenia Monge (CIBION-CONICET) – Dra. Silvia Moreno (CEQUIBIEM – CONICET-UBA).

 

Aplicaciones de espectrometría de masas en alimentos (8 horas)

Dr. Daniel Wunderlin – Dra. Ma. Verónica Baroni – Dra. Magdalena Monferrán Dra. Romina Di Paola (ICYTAC-CONICET-UNC)

 

BIBLIOGRAFÍA

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Protein kinase A regulatory subunit isoforms regulate growth and differentiation in Mucorcircinelloides: essential role of PKAR4. Ocampo, J.,  Mc Cormack, B., Navarro, E., Moreno, S., Garre,V. and Rossi, S. Eukaryotic Cell, 11 989-1002, 2012

PKA isoforms coordinate mRNA fate during nutrient starvationTudisca, V, Simpson, C., Castelli, L, Lui, J., Hoyle, N., Moreno, S., Ashe, M. and Portela, P.J. Cell Science, 125::5221-5232,  2012.

Activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinasesHaesendonckx S., Tudisca V, Voordeckers K., Moreno S., Thevelein J. and Portela, P.Biochem. J., 448: 307-320, 2012

Regulation of PKA activity by an auto-phosphorylation mechanism in Saccharomyces cerevisiae.Solari, C., Tudisca, V., Pugliessi, M., Nadra, A., Moreno, S. and Portela, P. Biochem. J.,  462: 567-579 , 2014.

Interacting proteins of protein kinase A regulatory subunit in Saccharomyces cerevisiae.Galello, F., Moreno, S. and Rossi, S.J. Proteomics, 109: 261-275, 2014

The PKA regulatory subunit from yeast forms a homotetramer: low-resolution structure of the N-terminal oligomerization domain.González Bardeci, N., Caramelo, J.J., Blumenthal, D.K., Rinaldi, J., Rossi, S. and Moreno, S. J. Structural. Biology (2015) enprensa, doi 10.1016/j.jsb.2015.12.001405 (2013) 7397-7404.

Determination of scandium in acid mine drainage by ICP-OES with flow injection on-line preconcentration using oxidized multiwalled carbon nanotubes. J. Jerez, A.C. Isaguirre, C. Bazán, L.D. Martinez, S. Cerutti. Talanta 124 (2014) 89 – 94.

Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS". Autores: S. Torres, S. Cerutti, J. Raba, P. Pacheco, M.F. Silva. Food Chemistry 159 (2014) 407-413.

Postharvest control of gray mould in apples with lyophilized formulations of Cryptococcus Laurentii: the effect of cold stress in the survival and effectiveness of the yeast .L.G. Navarta, J. Calvo, P. Posetto, S. Cerutti, J. Raba, D. Benuzzi, M.I. Sanz Ferramola. Food and Bioprocess Technology, 7 (2014) 2962-2968.

Characterization of Hg-phytochelatins complexes in vines (Vitisviniferacv Malbec) as defense mechanism against metal stress. A. Spisso, S. Cerutti, M. F. Silva, P.H. Pacheco, L.D. Martinez. BioMetals, 27 (2014) 591-599.

UHPLC-(+)APCI-MS/MS determination of oxygenated and nitrated polycyclic aromatic hydrocarbons in airborne particulate matter and tree barks collected in Buenos Aires city. F. Fujiwara, M.E. Guiñez, S. Cerutti, P. Smichowski. Microchemical Journal, 116 (2014)118-124.

Development of a LC–MS/MS methodology for the monitoring of the antichagasic drug benznidazole in human urine". N. Martínez, M.E. Marson, G.E. Mastrantonio, J. Raba, S. Cerutti. Talanta, 131 (2015) 656–660.

A Novel Solid Phase Extraction - Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry for the Quantification of Ochratoxin A in Red Wines. L. Mariño-Repizo, F. Kero, V. Vandell, A. Senior, S. Cerutti, J. Raba. Food Chemistry, 172 (2015) 663-668.

Vapor generation - atomic spectrometric techniques. Expanding frontiers through specific-species preconcentration. A review. Raúl A. Gil, Pablo H. Pacheco, S. Cerutti, Luis D. Martinez. AnalyticaChimicaActa 875 (2015) 7-21.

Solid phase extraction/cyclodextrin-modified micellar electrokinetic chromatography for the analysis of melatonin and related indole compounds in plants. Federico J.V. Gómez, I. Gatica Hernández, S. Cerutti, M.F. Silva. PublicadoenMicrochemical Journal 123 (2015) 22-27.

Melatonin in Arabidopsis thaliana acts as plant growth regulator at low concentrations and preserves seed viability at high concentrations. I. Gatica Hernández, Federico J.V. Gómez, S. Cerutti, M.F. Silva. Plant Physiology and Biochemistry 94 (2015) 191-196.

Metabolomic Serum Profiling Detects Early-Stage High-Grade Serous Ovarian Cancer in a Mouse Model. C.

Falsified medicines in Africa and public health – ‘No Action–Talk Only Jones, M. E. Monge, J. Kim, M. M. Matzuk, F. M. Fernández, Journal of Proteome Research 14 (2015) 917-927.

P. N. Newton, P. Tabernero, P. Dwivedi, M. J. Culzoni, M. E. Monge, I. Swamidoss, D. Mildenhall, M. D. Green, R. Jähnke, M. dos Santos de Oliveira, J. Simao, N. J White, F. M. Fernández, The Lancet Global Health 2 (2014) e509-e510.

Plasma-Spray Ionization (PLASI): A Multimodal Atmospheric Pressure Ion Source for Liquid Stream Analysis. A. Kaylor, P. Dwivedi, J. J. Pittman, M. E. Monge, G. Cheng, S. Li, F. M. Fernández, Journal of The American Society for Mass Spectrometry 25 (2014) 1788-1793.

Feasibility of Detecting Prostate Cancer by Ultraperformance Liquid Chromatography−Mass Spectrometry Serum Metabolomics. X. Zang, C. M. Jones, T. Q. Long, M. E. Monge, M. Zhou, L. D. Walker, R. Mezencev, A. Gray, J. F. McDonald, and F. M. Fernández, Journal of Proteome Research 13 (2014) 3444–3454.

A Tiered Analytical Approach for Investigating Poor Quality Emergency Contraceptives M. E. Monge, P. Dwivedi, M. Zhou, M. Payne, C. Harris, B. House, Y. Juggins, P. Cizmarik, P. N. Newton, F. M. Fernández, D. Jenkins, PLoS ONE (2014) 9(4):e95353. doi:10.1371/journal.pone.0095353.

Robotic Plasma Probe Ionization Mass Spectrometry (RoPPI-MS) of Non-Planar Surfaces. R. V. Bennett, E. M. Morzan, J. O. Huckaby, M. E. Monge, H. I. Christensen, F. M. Fernández, Analyst 139 (2014) 2658-2662.

Ion Mobility and Liquid Chromatography-Mass Spectrometry Strategies for Exhaled Breath Condensate Glucose Quantitation in Cystic Fibrosis Studies. M. E. Monge, J. J. Pérez, P. Dwivedi, M. Zhou, N. A. McCarty, A. A. Stecenko, F. M. Fernández, Rapid Communications in Mass Spectrometry 27 (2013) 2263-2271.

Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level. Roselló P., Vigliocco A., Andrade A., Riera N., Calafat M., Molas M.L., Alemano S. Plant PhysiolBiochem. 102: 133-140 (2016).

Alterations of endogenous hormonal levels in plants under drought and salinity. - Llanes A., Andrade A., Alemano S., Luna V. Aust. J. Bot. Enviado.

Drought and salinity alter endogenous hormonal profiles at the seed germination phase. -Llanes A., Andrade A., Masciarelli O., Alemano S., Luna V. Seed Sci. Res. (2015) doi: 10.1017/S0960258515000331.

Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences, and decreases bug attack. -Giacometti R., Barneto J., Barriga L.G., Sardoy P.M., Balestrasse K., Andrade A.M., Pagano E.A., Alemano S.G., Zavala JA.Pest Manag. Sci. (2015) doi: 10.1002/ps.4192.

Pericarp anatomy and hormone profiles of cypselas in dormant and non-dormant inbred sunflower lines.-Andrade A., Riera N., Lindstrom L., Alemano S., Alvarez D., Abdala G., Vigliocco A. Plant Biol. 17: 351-360 (2015).

Drought effects on the early development stages of Panicumvirgatum L.: Cultivar differences.Aimar D., Calafat M., Andrade A., Carassay L., Bouteau F., Abdala G., Molas L.  Biomass Bioenerg. 66: 49-59 (2014).

From Grape to Wine: Changes in Phenolic Composition and its Influence on Antioxidant Activity Food Chemistry. Lingua M.S., Fabani M.P., Wunderlin D.A., Baroni M.V. Food Chemistry (2016, enprensa).

Potential human health risks from metals and As via Odontesthesbonariensis consumption and ecological risk assessments in a eutrophic lake. Monferran M.V., Garnero P.L., Wunderlin D.A., Bistoni M.A. Ecotox. Environ. Safety (2016, enprensa).

Argentinean pistachio oil and flour: A potential novel approach of pistachio nut utilization. Feresin G.E., Martínez M., Fabani M.P., Baroni M.V., MagriniHuaman R., Ighani M., Wunderlin D.A., Tapia A.A. J. Food Sci. Technol. (2016, enprensa).

Bioconcentration of carbamazepine and other pharmaceuticals in fish under field and controlled laboratory experiments. Evidences of carbamazepine metabolization by fish. Valdés M. E., Huerta B., Wunderlin D.A., Bistoni M.A., Barceló D., Rodríguez-Mozaz S. Sci. Total Environ. 557–558, 58–67 (2016).

From Water to Edible Fish. Transfer of Metals and Metalloids in the San Roque Reservoir (Córdoba, Argentina). Implications Associated with Fish Consumption. Monferrán M.V., Garnero P., Bistoni M.A., Anbar A.A., Gordon G.W., Wunderlin D.A. Ecol. Ind. 63: 48-60 (2016).

Matching in vitro bioaccessibility of polyphenols and antioxidant capacity of soluble coffee by Boosted Regression Trees. Podio N.S., López-Froilán R., Ramirez-Moreno E., Bertrand L., Baroni M.V., Pérez-Rodríguez M.L., Cortes Sánchez-Mata M., Wunderlin D.A.* J. Agric. Food Chem. 63:(43), 9572-9582 (2015).

Dynamics of Glyphosate and AMPA in an agricultural watershed from the southeastern region of Argentina. Lupi L., Aparicio V., Marino D., Bedmar F., Wunderlin D.A., Miglioranza K. Sci. Total Environ. 536: 687–694 (2015).

Linking soil, water and honey composition to assess the geographical origin of Argentinean honey by multielemental and isotopic analyses. Baroni M.V., Podio N.S., Badini R.G., Inga M., Ostera H.A., Cagnoni M., Gautier E.A., Peral-García P., Hoogewerff J., Wunderlin D.A.* J. Agric. Food Chem. 63:(18), 4638–4645 (2015).

Screening concentration of E1, E2 and EE2 in sewage effluent and surface waters of the "Pampas" region and the "Río de la Plata" estuary (Argentina). Valdés M.E., Marino D.J., Wunderlin D.A., Somoza G. M., Ronco A.E., Carriquiriborde P. Bull. Environ. Contam. Toxicol. 94(1): 29-33 (2015).

Occurrence and Bioaccumulation of Pharmaceuticals in a Fish Species Inhabiting the Suquía River Basin (Córdoba, Argentina). Valdés M. E.; Amé M.V.; Bistoni, M.A.; Wunderlin, D.A.* Sci. Total Environ. 472: 389–396 (http://dx.doi.org/10.1016/j.scitotenv.2013.10.124) (2014).

Determination of Priority Pesticides in Water Samples Combining SPE and SPME Coupled To GC-MS. A Case Study: Suquía River Basin (Argentina). Bonansea, R.I.;Amé, M.V. and Wunderlin D.A.* Chemosphere 90: 1860-1869 (2013).

Arylation of aryl chlorides, a convenient method for the synthesis of new potential triazolic fungicides.Herrera Cano, N.;Santiago, A. N.*Tetrahedron.70, 280-285 (2014).

Synthesis and biological evaluation of benzothiazole derivates as antifungal agents.Herrera Cano, N.;Ballari,M. S.;Lopez, A. G.; Santiago A. N.* J. Agric. Food. Chem.63, 3681-3686 (2015).

Atmospheric Degradation of CH2=C(CH3)C(O)OCH3 initiated by OH Radicals: Mechanistic study and quantification of CH3C(O)C(O)OCH3 at NOx free air.Gibilisco,  R. G.; Uranga, J. G.; Santiago, A. N.; Teruel M. A. *J. Phys. Chem. A. 119, 8775–8783 (2015).

HS/SPME/GC-MS Determination of Volatile Organic Compounds (VOCs) in Andean Tomato Landraces.Cortina, P. R.; Asis, R.; Peralta I. E.; Asprelli, P.; Santiago A. N.*J. Brazil. Chem. Soc. Enprensa. (2016).

UV/H2O2degradation of the antidepressants venlafaxine and O-desmethylvenlafaxine: Elucidation of their transformation pathway and environmental fate. García-Galán, M.J., Anfruns, A., Gonzalez-Olmos, R., Rodríguez-Mozaz, S., Comas, J. (2016) Journal of Hazardous Materials, 311, pp. 70-80.

Fungal treatment for the removal of antibiotics and antibiotic resistance genes in veterinary hospital wastewater. Lucas, D., Badia-Fabregat, M., Vicent, T., Caminal, G., Rodríguez-Mozaz, S., Balcázar, J.L., Barceló, D.(2016) Chemosphere, 152, pp. 301-308.

Do pharmaceuticals bioaccumulate in marine molluscs and fish from a coastal lagoon?Moreno-González, R., Rodríguez-Mozaz, S., Huerta, B., Barceló, D., León, V.M.(2016) EnvironmentalResearch, 146, pp. 282-298.

Suspect screening of emerging pollutants and their major transformation products in wastewaters treated with fungi by liquid chromatography coupled to a high resolution mass spectrometry. Llorca, M., Lucas, D., Ferrando-Climent, L., Badia-Fabregat, M., Cruz-Morató, C., Barceló, D., Rodríguez-Mozaz, S.(2016) Journal of Chromatography A, 1439, pp. 124-136.

Advanced oxidation of the antibiotic sulfapyridine by UV/H2O2: Characterization of its transformation products and ecotoxicological implications. García-Galán, M.J., Anfruns, A., Gonzalez-Olmos, R., Rodriguez-Mozaz, S., Comas, J.(2016) Chemosphere, 147, pp. 451-459.

Occurrence and persistence of antibiotic resistance genes in river biofilms after wastewater inputs in small rivers. Proia, L., Von Schiller, D., Sànchez-Melsió, A., Sabater, S., Borrego, C.M., Rodríguez-Mozaz, S., Balcázar, J.L. (2016) Environmental Pollution, 210, pp. 121-128.

Internal exposure dynamics drive the Adverse Outcome Pathways of synthetic glucocorticoids in fish. Margiotta-Casaluci, L., Owen, S.F., Huerta, B., Rodríguez-Mozaz, S., Kugathas, S., Barceló, D., Rand-Weaver, M., Sumpter, J.P. (2016) Scientific Reports, 6, art. no. 21978, .

Non conventional biological treatment based on Trametes versicolor for the elimination of recalcitrant anticancer drugs in hospital wastewater. Ferrando-Climent, L., Cruz-Morató, C., Marco-Urrea, E., Vicent, T., Sarrà, M., Rodriguez-Mozaz, S., Barceló, D. (2016) Chemosphere, 136, pp. 9-19.