Datos de investigaciones de los proyectos PICT 2019-1180 y PIP 0761 El objetivo general de este proyecto es estudiar el efecto del antiparasitario externo fluazurón (FLZ) principio activo (p.a.) y de un formulado comercial, en cultivos primarios de células somáticas de oviducto y en la maduración in vitro (MIV) de ovocitos bovinos, analizando diferentes biomarcadores de efecto genotóxico, citotóxico y fisiológico. Nuestra hipótesis es que FLZ induce daño genotóxico, citotóxico, altera la fisiología de las células oviductales y el desarrollo normal de la maduración del ovocito bovino impactando en el desarrollo embrionario preimplantacional in vitro

Secuencias 16S rRNA e ITS de la comunidad de bacterias y hongos del Río Amarillo y los cultivos asociados Secuencias 16S ARN ribosómico región V3-V4 Secuencias fúngicas región ITS 1y ITS2 Obtenidas mediante secuenciación Illumina MySeq platform mediante los servicios de Molecular Research LP Mr DNA (Shallowater, TX, USA).

Experimentos para la reacción de RWGS sobre catalizador de Pd 0.08%pp -Ce 3,84%pp para una mezcla de alimentación CO2/H2/CH4/CO/ Ar a P=1.6 bar Este trabajo de recolección y análisis de datos se enfoca específicamente en la influencia sobre la reacción RWGS de la cantidad de CO en la alimentación. Se ha realizado un estudio cinético de un catalizador de laboratorio Ce-Pd/Al2O3 a 600oC, desarrollado en PLAPIQUI. Con estos experimentos, se proyecta investigar la influencia de los reactivos (H2 y CO2) y productos de la reacción (CO, H2O) con el propósito de generar una correlación de la velocidad de reacción para altas temperaturas. Los experimentos para el estudio cinético se llevaron a cabo en un reactor tubular de lecho fijo localizado en un laboratorio del PLAPIQUI. Para la recopilación de datos experimentales se utilizó un tubo de cuarzo de 1 m de longitud con un diámetro interno de 10 mm, ubicado dentro de un horno eléctrico Lindberg-Blue M con tres zonas de calentamiento independientes. Cargas de catalizador entre 30 y 80 mg se colocaron en el tubo entre tapones de lana de cuarzo. El reactor es alimentado por tubos de gas de CH4, H2, CO2 y Ar regulados por un controlador y sus respectivos caudalímetros. El flujo de salida del reactor se dirige hacia una trampa de agua (lecho fijo de sílica gel y tamiz molecular) y luego se analiza en un cromatógrafo de gases Perkin Elmer Autosystem utilizando argón como gas portador y operado con un detector de conductividad térmica (TCD). Antes de cada experimento, el catalizador fresco se reduce con un flujo de hidrógeno durante media hora.

Datos de localidades de ocurrencia de A. quebracho-blanco Coordenadas de ocurrencia recopiladas de datos de colectas propias del grupo de trabajo, datos de herbario y bases de datos online de A. quebracho-blanco para la construcción de Modelos de nicho ecológico y análisis espaciales, asociados con datos genéticos.

Pollen types identified in honey samples of Plebeia mansita and their frequency classes and frequency of occurrence in locality Baritú, Salta province La información aportada en esta base corresponde a los datos recopilados durante la observación microscópica de las 41 muestras de miel de Plebeia mansita, una abeja nativa sin aguijón productora de miel en sectores de Yungas del Noroeste Argentino. Los datos corresponden a los valores porcentuales registrados de cada uno de los tipos polínicos observados e identificados en las muestras de miel. Asimismo, la base aporta datos sobre la riqueza polínica (valores mínimo, máximo y promedio) observada en cada muestra de miel y la clasificación asignada a cada muestra, es decir si la misma es una miel monofloral o multifloral de acuerdo a la reglamentación vigente en el territorio argentino.

Datos de parámetros Fisico químicos de sitios de muestreo 2023 Datos de parámetros físico químicos de sitios muestreados en el Río Luján y el Río Areco en San Antonio de Areco. Recuento de micronúcleos para los sitios del río Luján y determinación de factor de condición para ambas cuencas

Estres salino e hídrico en Trichloris Variables morfofisiológicas evaluadas en Marinoni et al. (2020). Evaluación de variables morfológicas y fisiológicas en respuesta a estres salino e hídrico en ecotipod de T. crinita y T. pluriflora

Caracterización de NPs de YVO4:Eu3+ funcionalizacionalizadas con ácido oleico y 3-(trimetoxisilil)propil metacrilato Se caracterizaron nanopartículas de YVO4:Eu3+ funcionalizadas con dos agentes de acoplamiento distintos: 3-(trimetoxisilil)propil metacrilato y ácido oleico. A las nanopartículas funcionalizadas con ácido oleico previamente se les sintetizó un shell de SiO2. Las técnicas de caracterización utilizadas fueron: FTIR, TEM, TGA y DLS.

Emisiones netas de metano desde un relleno sanitario en la ciudad de Tandil, Argentina El conjunto de datos contiene las concentraciones de metano atmosférico (CH4) y las emisiones de CH4 estimadas de un vertedero en la ciudad de Tandil, Argentina. Las concentraciones atmosféricas de CH4 (en ppm) se registraron durante 10 períodos de 15 días cada uno. Las muestras de aire se recolectaron mediante recipientes de acero inoxidable con un volumen de 0.5 litros, que comprenden una válvula de entrada y un restrictor de ingreso de aire. Además, los flujos de CH4 desde la superficie de las celdas del vertedero (en g m-2 d-1) se midieron aplicando la técnica de cámara estática. Finalmente, se muestran las emisiones de CH4 de los tubos de venteo del relleno (en g d-1), estimados utilizando la metodología de trazadores de gas con cápsulas permeables llenas de SF6 instaladas dentro de cada tubo de venteo. Todos estos datos nos permitieron estimar las emisiones netas de CH4 del relleno estudiado y la energía eléctrica potencial que podría generarse a partir del CH4 liberado de los tubos de venteo.

Revisión de literatura de distribucuión geográfica a nivel mundial y arreglo experimental para medición de Tasa de Oxidación de metano (TOM) en suelos con forestaciones, hasta diciembre de 2021 Recopilación de datos a partir de literatura científica en idioma inglés hasta diciembre de 2021, en la que se estima la Tasa de oxidación de metano (TOM) en sistemas forestales (naturales o implantados) y en ocasiones, otros sistemas de referencia como pastizales, cultivos, rellenos sanitarios, arrozales. Se presenta listado de autores, ubicación geográfica de los registros, valor de TOM reportado, condiciones de medición y valor obtenido de TOM.

Presencia/Ausencia de especies/morfo-especies de arañas en agroecosistemas del Chaco Seco Meridional Se trata de una base de datos de presencias (1) y ausencias (0) creada con colectas realizadas entre mediados de febrero y mediados de marzo de 2011, 2012 y 2013 en agroecosistemas del Chaco Seco, en el Noroeste de la provincia de Córdoba

Shorelines and beach width time series for three beaches of Mar del Plata (1986-2021) acquired from satellite imagery This repository contains the data used to evaluate the performance of a beach nourishment project in three bays of Mar del Plata, Province of Buenos Aires, Argentina. The project was carried out by the Belgian company Dredging International between 1998 and 1999. A total of 2,480,000 m3 of sediments were dredged from the mouth of the local port and deposited on the Playa Grande, Varese and Bristol beaches. CoastSat 2.0 toolkit (https://github.com/kvos/CoastSat), an open-source Python software for shoreline detection was utilized. The toolkit allows users to acquire time series of shoreline positions for any coastal area using available satellite imagery from the Google Earth Engine platform. In this case, it was used with data from the Landsat missions L5 (1986–2012), L7 (1999–2021), L8 (2013–2021), and the Sentinel mission S2 (2015–2021). Top-of-Atmosphere reflectance images from the Landsat missions with a resolution of 30 m and a revisit time of 16 days (Tier 1) were utilized, along with images from the Sentinel 2 mission with a resolution of 10 m and a revisit time of 5 days (Level-1C). Additionally, the toolkit employed spatial resolution enhancement techniques over Landsat images to map the position of the shoreline with an accuracy of ~10 m. In this repository, CoastSat-detected shorelines can be accessed along with the normal to shore transects from which the beach width time series were obtained to analyze beach response to nourishment. Tide-corrected beach width time series are also provided.

Thermophysical Behavior of Carbonated Aqueous Solutions Containing Monoethanolamine and Degradation Products Hernández Narciso, Clàudia Rosa; Martínez, Cristina G.; O'Connell, Brendan; Rodriguez Reartes, Sabrina Belen; Llovell, Fèlix; Trusler, J. P. Martin; Sedransk Campbell, Kyra L. The impact of the degradation of monoethanolamine (MEA) on the physicochemical properties of the solvent is experimentally characterized. Based on the identification of three main degradation products of MEA: oxazolidine-2-one (OZD), N-(2-hydroxyethyl)ethylenediamine (HEEDA), and 1-(2-hydroxyethyl)-2-imidazolidinone (HEIA), new measurements for the density, surface tension, and viscosity of partially carbonated solutions containing water, MEA and those products were conducted at different MEA/degradation product molar ratios. The experiments covered a temperature range from 298.15 to 353.15 K at atmospheric pressure. The more stable and impactful degradation product, HEIA, was analyzed separately to determine its vapor pressure, as well as the density and viscosity of aqueous solutions with HEIA mass fractions of 100, 75, 50, and 25% in the same temperature range. The reported data demonstrate the difference in the performance of aqueous MEA solutions containing degradation products as compared to a fresh solution. This aspect is crucial for understanding the impact and effectiveness of postcombustion CO2 capture using aqueous amine systems in an industrial setting.

Electric Field Gradient in Chiral and Tetrahedral Molecules within High-Order LRESC Formalism Aucar, Juan Jose; Melo, Juan Ignacio; Maldonado, Alejandro Fabián In this work, we present the electric field gradient (EFG) given by the linear response elimination of the small component (LRESC) scheme up tothe 1/c order (c is the speed of light in vacuum) in CHFClX (X = Br, I, At) chiral molecules, together with CHF2Br and CH2FX (X = Br, I, At) tetrahedral systems. The former could be good candidates for further parity violation studies, especially when heavy atoms are surrounding. In this context, the LRESC scheme demonstrates effective applicability to large tetrahedral and chiral molecules that incorporate heavy elements, with relativistic effects playing a crucial role. The LRESC results of EFG exhibit an excellent agreement with those calculated at the four-component level, giving differences of only hundredths order in a.u. (atomic units) for the bromine nucleus and less than 0.1 a.u. for the iodine nucleus.Regarding the other nuclei, for the chiral molecules, there is a heavy atom effect on the light atom (HALA) for chlorine and fluorine atoms as the substituent halogen atom becomes heavier. Furthermore, the electronic part of the EFG for the central carbon and fluorine nuclei presents an important dependence with the environment in the molecules under study. With accurate calculations of the EFG and tabulated nuclear quadrupole moment, the nuclear quadrupole coupling constant is obtained within the LRESC scheme, including for the first time correlation effects on the spin-dependent corrections with this methodology, providing results close to the experimental ones for Cl, Br, and I atoms. At the Hartree−Fock level, the differences are around 6% for Br and I nuclei, and at the density functional theory level with the LDA and PBE0 functionals, the differences are no more than 2%.

A mini review of mycotoxin’s occurrence in food in South America in the last 5 years: Research gaps and challenges in a climate change era Foerster, Claudia; Müller Sepúlveda, Andrea; Copetti, Marina Venturini; Arrua, Andrea Alejandra; Monsalve, Liliam; Ramirez, Maria Laura; Torres, Adriana Mabel Mycotoxins are natural metabolites produced by species of filamentous fungi belonging mainly to the genera Aspergillus, Fusarium, Penicillium, and Alternaria, which can grow in various crops and foodstuffs. The South American climate is diverse, varying from tropical, temperate, and arid to cold, ideal for the growth of different types of fungi and mycotoxin production. This mini review aimed to describe the natural occurrence of mycotoxin in food in South America from 2018 to 2023, identifying research gaps and challenges in an era of climate change. We analyzed 53 studies, 21 from Brazil. Most of the mycotoxins analyzed in South America were the traditional and regulated mycotoxins, with variable occurrences depending on the region, climatic conditions, and methodology used. Emerging and modified mycotoxins have only been studied in Argentina and Brazil, where some studies have shown high occurrences. Given this, it is essential to strengthen food safety laboratories and surveillance capabilities and establish early warning systems. It is also essential to continue working to raise awareness of mycotoxins as a public health issue and to study and prevent the impact of climate change on soil microbial population, the new prevalence of fungi, and the profile of toxigenic species. An effective connection and collaboration between disciplines and sectors in different countries is needed to meet this research challenge.

Influencia del reloj biológico sobre la expresión de genes relacionados con la resistencia a insecticida en Triatoma Infestans En Triatoma infestans se observó resistencia a los piretroides atribuida en parte a una metabolismo oxidativo elevado mediado por los citocromos P450. La nicotinamida Adenina dinucleótido fosfato (NADPH) citocromo P450 reductasa (CPR) desempeña un papel crucial en catalizar la transferencia de electrones del NADPH a todos los citocromos P450. Las variaciones diarias en la expresión del gen CPR y de un gen P450 (CYP4EM7), ambos asociado con la resistencia a los insecticidas, sugirió que sus expresiones estarían bajo el control del reloj endógeno. Para aclarar la participación del reloj en la orquestación de las fluctuaciones diarias en la expresión de los genes CPR y CYP4M7, se propuso investigar el efecto de silenciar el gen del reloj period (per) por ARN interferencia (ARNi). Los resultados obtenidos permitieron establecer que el silenciamiento del per es influenciado por los esquemas de ingesta utilizados en los protocolos de interferencia. El el silenciamiento de per gen en T. infestans redujo su expresión en todos los puntos de tiempo analizados y abolió el ritmo característico en la expresión transcripcional del ARNm del gen per. El efecto del silenciamiento del gen per en los perfiles de expresión a nivel transcripcional de los genes CPR y CYP4EM7 mostró la pérdida de ritmicidad y demostró la participación del reloj biológico en la regulación de su expresión.

Parasite assemblages as indicators of stability in stock structure of Cynoscion guatucupa (Sciaenidae) after a quarter of century of exploitation in a marine warming hotspot Lanfranchi, Ana Laura; Canel, Delfina; Alarcos, Ana Julia; Levy, Eugenia; Braicovich, Paola Elizabeth; Marcotegui, Paula Soledad; Timi, Juan Tomas Climate change is impacting marine species, leading to shifts in their distribution to higher latitudes and to deeper locations. Parasite communities are dynamic systems influenced by biotic and abiotic factors, making them valuable tools for studying ecological processes of their hosts. This work aims to assess possible changes in parasite structure that occurred in the northern stock of Cynoscion guatucupa in the Argentine Sea, over approximately 25 years, by using parasites as tags in a region characterized as a hotspot of significant warming in recent decades. Parasite assemblages of adult and juvenile fish from two periods caught at two regions of the Argentine Sea were analyzed. Species richness remained relatively constant across samples, although four of the eight compared species from juvenile fish showed significant increases in prevalence, while for adults three species decreased. Differences in parasite assemblages were significant between periods, but not between regions, indicating that the stock structure has apparently remained stable in recent years, even under a warming process that, in turn, seems to have affected its parasite assemblages which however, continue showing homogeneous parasitological attributes throughout the habitats. Most results indicate that changes in parasite burdens are not related to the population density or geographic distribution of C. guatucupa, but to changes in temperature, salinity or in the density of top predators. Parasites seem to have felt the impact of global change before their host, thus, they can be considered early sentinels to monitor the stability and distribution of this important resource.

Growing in Scarcity: Pre-Hispanic Rain-Fed Agriculture in the Semi-Arid and Frost-Prone Andean Altiplano (Bolivia) Cruz, Pablo; Joffre, Richard; Saintenoy, Thibault; Vacher, Jean Joinville Ancient Andean agricultural landscapes have been the subject of a large number of archaeological and agro-ecological studies, which generally refer to regions with favourable environmental conditions or, in the case of arid and semi-arid environments, those with irrigation facilities. The aim of this article is to present and analyse the pre-Hispanic rain-fed farming systems widely represented in two adjacent regions of Bolivia’s arid and cold southern Altiplano. The search for archaeological agricultural areas combined aerial analysis and field surveys. Agro-ecological characterisation was based on historical and ethnographic studies of the region’s present-day populations. Despite their geographical proximity, similar environmental conditions, and same agropastoral way of life, the typology of cultivated areas developed in the southern altiplano differs significantly. Within this same framework of adaptation and resilience, the sectorisation of agricultural systems observed in these two regions reveals a regional productive specialisation that favoured internal exchanges and exchanges with other regions. These differences are related to two models of non-centralised, low-inequality societies—one strongly based on cohesion and the other characterised by greater fragmentation and social conflict—underlining the limits of strict environmental determinism in shaping agricultural landscapes. These results provide new food for thought in the debate on the use and value of rain-fed agricultural practices and more broadly on the diversity of adaptations by human societies in extreme and unstable environmental contexts.

δ18O variability in guanaco bone bioapatite in Southern Patagonia: Implications for paleoecological and paleoenvironmental studies Merler Carbajo, Julia; Dellepiane, Juan Matias; Morales, Marcelo Raul; Goñi, Rafael Agustín; Tessone, Augusto The guanaco (Lama guanicoe) is the largest and the most widely distributed ungulate in South America since the Pleistocene and constituted the main prey for hunter-gatherers in Southern Patagonia (Argentina) in the past. An isomorphic relationship has been suggested between the mobility of these animals and that of human groups that inhabited the region, which presents altitudinal differences. On the one hand, we seek to understand the spatial dynamics of guanaco populations and their interaction with hunter-gatherer societies. On the other hand, to evaluate the utility of archaeological herbivores' values as paleoenvironmental proxies that allow addressing the environmental variations during the Holocene in the region. We present the results of the analysis of δ18O in bioapatite of Lama guanicoe bone specimens from the area located in the central-western area of the province of Santa Cruz, between 70 and 72 degrees west longitude. We analyzed 41 samples. 22 of them correspond to modern individuals, while the remaining 19 were recovered from Holocene archaeological sites. The carbonate-phosphate and estimated water equations will be used to compare with pre-existing data of δ18O in the waters of the region. Results showed that the mean value of δ18O of the pooled samples was −6.5 ‰ ± 1 ‰. Even though some differences were detected, both, between archaeological vs. modern and between highlands vs. lowlands samples, no statistically significant differences were obtained in either case. In general terms, the data respond to what was expected regarding the complex nature of the water intake behaviors of these animals. The data presented in this paper constitutes a first approximation to the expected values of δ18O for guanacos in the region, its relationship with the hydrological cycle and human populations.

Revisiting the Cretaceous Lungfish atlantoceratodus Iheringi (Ameghino 1898) from the Mata Amarilla formation (Argentina) with comments on tooth plates histology Panzeri, Karen Magalí Atlantoceratodus iheringi (Ameghino, 1898) from Argentine territory is restudied based on its known tooth plates and newly discovered material, which includes previously unknown skull roof bones and vomerine tooth plates. The latter represent the first records of such elements from the Mesozoic era in South America. The comparative morphological analysis reveals its distinctiveness from other dipnoans, and offers valuable data for future systematic and phylogenetic research. The pterygopalatine tooth plates display narrow-based first denticulations and lack anterior wear facets, with the inner angle positioned at the level of the second denticulation. Similarly, the prearticular tooth plates feature straight mediolingual edges, and a wide-based first denticulation without sinuosities at the tip. Histological sections are performed and analyzed in detail for the first time. A. iheringi presents in this aspect distinctive features such as: large-lumen denteons tending to cluster together, circumdenteonal dentine arranged in a double band (an inner birefringent and an outer monorefringent), and a disordered interdenteonal dentine, with birefringence surrounding denteons and areas with monorefringence. A. iheringi exhibits histological structure closer to Mesozoic and Cenozoic dipnoans than Paleozoic, especially resembling the disposition observed in the Upper Cretaceous Patagonian species Metaceratodus baibianorum. The wide distribution of features designated as diagnostic for Atlantoceratodus is discussed.