Sindicador de canales de noticias

Heterogeneous SoC-based acceleration of MPEG-7 compliance image retrieval process

CONICET Digital -

Heterogeneous SoC-based acceleration of MPEG-7 compliance image retrieval process Molina, Romina Micaela; Dondo Gazzano, Julio Daniel; Rincon, Fernando; Gil Costa, Graciela Verónica; Barba, Jesus; Petrino, Ricardo; Lopez, Juan Carlos With the growing amount of multimedial content over the internet and broadcast systems, mechanisms for efficient information organization, manipulation and transmission are becoming indispensable. Optimization of the multimedia search and retrieval processes is nowadays an important area of development due to the difficulty to browse, filter and manage that big amount of data. The adoption of the MPEG-7 standard has a significant importance to simplify the image retrieval process. However, performance issues are still relevant when the retrieval must be accomplished in real time. This work presents an innovative and efficient approach of a Content-Based Retrieval Process using metric spaces implemented in heterogeneous resources according to the demand of computational power. Several implementations were made and comparative results are shown evidencing the benefits of the proposed approach.

Aporte a la cronología de chenques tardíos del Lago Salitroso (Santa Cruz, Argentina)

CONICET Digital -

Aporte a la cronología de chenques tardíos del Lago Salitroso (Santa Cruz, Argentina); Contribution to the chronology of late Chenques from Lake Salitroso (Santa Cruz, Argentina) Garcia Guraieb, Solana; Goñi, Rafael Agustín; Guichon, Rocio Se presentan dos nuevos fechados radiocarbónicos obtenidos en uno de los chenques tardíos (ca. 800-350 AP) del lago Salitroso (SAC) y sus implicaciones para las discusiones vigentes sobre prácticas mortuorias y cuestiones demográficas en el área. Se trata de SAC 1-2, un chenque primario múltiple, compuesto por un adulto femenino y 5 individuos infantiles. Dos fechados anteriores lo situaban en el orden de ca. 450 años AP. Actualmente, se fecharon dos subadultos hallados juntos en la base del entierro, cuya posición sugería su inhumación contemporánea. Mientras uno de los individuos mantuvo el orden de los ca. 400-500 años AP (487 ± 23 AP), el segundo presenta una fecha (709 ± 30 AP) que se aleja más de 200 años radiocarbónicos del anterior. Esta situación genera, nuevas preguntas sobre las prácticas mortuorias y la manera en que ocurre la reutilización de las estructuras de entierro. Además, tiene implicaciones para ideas previas sobre la recurrencia de episodios de simultaneidad de muerte de subadultos de diversas edades como explicación posible para las “anomalías” identificadas en el perfil de mortalidad de los chenques tardíos. Este caso alerta sobre la necesidad de refinar aún más el detalle de la cronología de los entierros del lago Salitroso.; In this article, two new radiocarbon dates from a late chenque (ca. 800-350 AP) at Lake Salitroso (SAC) are presented. These dates have implications for current discussions concerning mortuary practices and demography in the region. The case discussed is chenque SAC 1-2, a multiple individual, primary burial, containing one female adult and 5 infants. Two prior radiocarbon dates date this burial to ca. 450 BP. The two new radiocarbon dates were from two sub-adults placed next to each other at the base of the burial. Their positions suggest contemporaneous inhumation. Yet, while the date from one of the individuals (487 ± 23 BP) was within the same chronological range of ca. 400-500 BP, the other individual yielded a date –709 ± 30 AP– set approximately 200 years earlier. This chronological discrepancy raises new questions concerning mortuary practices and the way in which burial structures were reused in the region. It also has implications for existing hypotheses regarding the reoccurrence of mortality events that affected coevally sub-adults of different ages. This has been posed as a possible explanation for the abnormal pattern observed in the mortality profile of the late chenques. Our case-study warns us on the need to further refine the chronology of human burials from Lake Salitroso.

Extracting chemical information from high-resolution Kβ X-ray emission spectroscopy

CONICET Digital -

Extracting chemical information from high-resolution Kβ X-ray emission spectroscopy Limandri, Silvina Paola; Robledo, José Ignacio; Tirao, German Alfredo High-resolution X-ray emission spectroscopy allows studying the chemical environment of a wide variety of materials. Chemical information can be obtained by fitting the X-ray spectra and observing the behavior of some spectral features. Spectral changes can also be quantified by means of statistical parameters calculated by considering the spectrum as a probability distribution. Another possibility is to perform statistical multivariate analysis, such as principal component analysis. In this work the performance of these procedures for extracting chemical information in X-ray emission spectroscopy spectra for mixtures of Mn2+ and Mn4+ oxides are studied. A detail analysis of the parameters obtained, as well as the associated uncertainties is shown. The methodologies are also applied for Mn oxidation state characterization of double perovskite oxides Ba1+xLa1−xMnSbO6 (with 0 ≤ x ≤ 0.7). The results show that statistical parameters and multivariate analysis are the most suitable for the analysis of this kind of spectra.

Programming of the reproductive axis by hormonal and genetic manipulation in mice

CONICET Digital -

Programming of the reproductive axis by hormonal and genetic manipulation in mice Rulli, Susana Beatriz; Cambiasso, Maria Julia; Ratner, Laura Daniela In mammals, the reproductive function is controlled by the hypothalamic-pituitary-gonadal axis. During development, mechanisms mediated by gonadal steroids exert an imprinting at the hypothalamic-pituitary level, by establishing sexual differences in the circuits that control male and female reproduction. In rodents, the testicular production of androgens increases drastically during the fetal/neonatal stage. This process is essential for the masculinization of the reproductive tract, genitals and brain. The conversion of androgens to estrogens in the brain is crucial for the male sexual differentiation and behavior. Conversely, feminization of the brain occurs in the absence of high levels of gonadal steroids during the perinatal period in females. Potential genetic contribution to the differentiation of brain cells through direct effects of genes located on sex chromosomes is also relevant. In this review, we will focus on the phenotypic alterations that occur on the hypothalamic-pituitary-gonadal axis of transgenic mice with persistently elevated expression of the human chorionic gonadotropin hormone (hCG). Excess of endogenously synthesized gonadal steroids due to a constant hCG stimulation is able to disrupt the developmental programming of the hypothalamic-pituitary axis in both transgenic males and females. Locally produced estrogens by the hypothalamic aromatase might play a key role in the phenotype of these mice. The 'four core genotypes' mouse model demonstrated a potential influence of sex chromosome genes in brain masculinization before critical periods of sex differentiation. Thus, hormonal and genetic factors interact to regulate the local production of the neurosteroids necessary for the programming of the male and female reproductive function.

Engineering a bifunctional copper site in the cupredoxin fold by loop-directed mutagenesis

CONICET Digital -

Engineering a bifunctional copper site in the cupredoxin fold by loop-directed mutagenesis Espinoza Cara, Andrés Matías; Zitare, Ulises Alejandro; Álvarez Paggi, Damián Jorge; Klinke, Sebastian; Otero, Lisandro Horacio; Murgida, Daniel Horacio; Vila, Alejandro Jose Copper sites in proteins are designed to perform either electron transfer or redox catalysis. Type 1 and CuA sites are electron transfer hubs bound to a rigid protein fold that prevents binding of exogenous ligands and side reactions. Here we report the engineering of two Type 1 sites by loop-directed mutagenesis within a CuA scaffold with unique electronic structures and functional features. A copper-thioether axial bond shorter than the copper-thiolate bond is responsible for the electronic structure features, in contrast to all other natural or chimeric sites where the copper thiolate bond is short. These sites display highly unusual features, such as: (1) a high reduction potential despite a strong interaction with the axial ligand, which we attribute to changes in the hydrogen bond network and (2) the ability to bind exogenous ligands such as imidazole and azide. This strategy widens the possibility of using natural protein scaffolds with functional features not present in nature.

Critical frequencies of the ionospheric F1 and F2 layers during the last four solar cycles: Sunspot group type dependencies

CONICET Digital -

Critical frequencies of the ionospheric F1 and F2 layers during the last four solar cycles: Sunspot group type dependencies Yiğit, Erdal; Kilcik, Ali; Elias, Ana Georgina; Dönmez, Burçin; Ozguc, Atila; Yurchshyn, Vasyl; Rozelot, Jean Pierre The long term solar activity dependencies of ionospheric F1 and F2 regions’ critical frequencies (f0F1 and f0F2) are analyzed for the last four solar cycles (1976–2015). We show that the ionospheric F1 and F2 regions have different solar activity dependencies in terms of the sunspot group (SG) numbers: F1 region critical frequency (f0F1) peaks at the same time with the small SG numbers, while the f0F2 reaches its maximum at the same time with the large SG numbers, especially during the solar cycle 23. The observed differences in the sensitivity of ionospheric critical frequencies to sunspot group (SG) numbers provide a new insight into the solar activity effects on the ionosphere and space weather. While the F1 layer is influenced by the slow solar wind, which is largely associated with small SGs, the ionospheric F2 layer is more sensitive to Coronal Mass Ejections (CMEs) and fast solar winds, which are mainly produced by large SGs and coronal holes. The SG numbers maximize during of peak of the solar cycle and the number of coronal holes peaks during the sunspot declining phase. During solar minimum there are relatively less large SGs, hence reduced CME and flare activity. These results provide a new perspective for assessing how the different regions of the ionosphere respond to space weather effects.

Determination and Analysis of Residual Stresses Induced by High Speed Milling Using a Micro-indent Method

CONICET Digital -

Determination and Analysis of Residual Stresses Induced by High Speed Milling Using a Micro-indent Method Vottero, S.; Diaz, Felipe Victor; Mammana, Claudio Alejandro; Guidobono, A. The purpose of this work is to determine and analyze residual stress normal components and anisotropy degrees introduced by high-speed milling in specimens of AA 6082-T6 and AA 7075-T6 aluminum alloys. At each machined sample, the climb and conventional cutting zones were evaluated and compared. This paper includes a comprehensive study of thermal and mechanical effects associated with the residual stress introduction. For normal components determination, an optimized micro-indent method was used. Each measurement sequence from this approach was performed using a high accuracy measuring machine and classified according to thermal deviations measured. The residual displacements were determined with an absolute error down to ±300 nm. The normal components analysis allowed to infer the strong influence of the rolling process previous to high-speed milling and besides, the stress levels associated with thermal effects (higher in AA 7075-T6). Finally, the lower residual stress anisotropy degrees in both materials observed in the conventional cutting zone would indicate more homogenous local plastic stretching in this region for all planar directions.

Conformal Killing-Yano 2-forms

CONICET Digital -

Conformal Killing-Yano 2-forms Dotti, Isabel Graciela; Andrada, Adrián Marcelo Riemannian manifolds carrying 2-forms satisfying the Killing-Yano equation and the conformal Killing-Yano equation are natural generalizations of nearly Kähler and Sasakian manifolds. In this article we exhibit new solutions of these equations. We also provide obstructions for their existence on Lie groups, and reduce the study of conformal Killing-Yano 2-forms to a particular class of non degenerate Killing-Yano 2-forms.

La verdad burocratizada: las condiciones materiales de vida de los internos en la Penitenciaría de Córdoba entre 1908 y 1916

CONICET Digital -

La verdad burocratizada: las condiciones materiales de vida de los internos en la Penitenciaría de Córdoba entre 1908 y 1916; The bureaucratized truth: the material conditions of life of the inmates in the Córdoba Prison between 1908 and 1916 Luciano, Milena Este artículo aborda los lineamientos establecidos en la documentación oficial con respecto a las condiciones de alimentación, vestuario e higiene de los presos de la Penitenciaria de Córdoba. Específicamente analizamos la relación entre las versiones difundidas en el discurso oficial y las denuncias efectuadas por parte de los medios de prensa locales, durante la gestión de Antonio Amaya (1908 – 1916). Por último recuperamos la sublevación ocurrida en el penal en mayo de 1916, como acontecimiento central que, con la destitución de su director, cristalizó las contradicciones existentes al interior de la Penitenciaría.; This article addresses the guidelines established in the official documentation regarding the feeding, clothing and hygiene conditions of the prisoners in the Córdoba Prison. Specifically, we analyze the relationship between the versions disseminated in the official speech and the denunciations made by the local press during the administration of Antonio Amaya (1908 - 1916). Finally we recover the riot that took place in the prison in May 1916, as a central event that caused the dismissal of its director, crystallized the contradictions existing inside the prison.

Developing a novel paper-based enzymatic biosensor assisted by digital image processing and first-order multivariate calibration for rapid determination of nitrate in food samples

CONICET Digital -

Developing a novel paper-based enzymatic biosensor assisted by digital image processing and first-order multivariate calibration for rapid determination of nitrate in food samples Jalalvand, Ali R.; Mahmoudi, Majid; Goicoechea, Hector Casimiro For the first time, a novel analytical method based on a paper based enzymatic biosensor assisted by digital image processing and first-order multivariate calibration has been reported for rapid determination of nitrate in food samples. The platform of the biosensor includes a piece of Whatman filter paper impregnated with Griess reagent (3-nitroaniline, 1-naphthylamine and hydrochloric acid) and nitrate reductase. After dropping a distinct volume of nitrate solution onto the biosensor surface, nitrate reductase selectively reduces nitrate to nitrite and then the Griess reagent selectively reacts with nitrite to produce a red colored azo dye. Therefore, the color intensity of the produced azo dye is correlated with nitrate concentration. After image capture, the images were processed and digitized in the MATLAB environment by the use of an image processing toolbox and the vectors produced by the digital image processing step were used as inputs of the first-order multivariate calibration algorithms. Several multivariate calibration algorithms and pre-processing techniques have been used to build multivariate calibration models for verifying which technique offers the best predictions towards nitrate concentrations in synthetic samples and the best algorithm has been chosen for nitrate determination in potato, onion, carrot, cabbage and lettuce samples as real cases.

UV-B radiation delays flowering time through changes in the PRC2 complex activity and miR156 levels in Arabidopsis thaliana

CONICET Digital -

UV-B radiation delays flowering time through changes in the PRC2 complex activity and miR156 levels in Arabidopsis thaliana Dotto, Marcela Claudia; Gomez, Maria Sol; Soto, María Soledad; Casati, Paula UV-B is a high-energy component of the solar radiation perceived by the plant and induces a number of modifications in plant growth and development, including changes in flowering time. However, the molecular mechanisms underlying these changes are largely unknown. In the present work, we demonstrate that Arabidopsis plants grown under white light supplemented with UV-B show a delay in flowering time, and this developmental reprogramming is mediated by the UVR8 photoreceptor. Using a combination of gene expression analyses and UV-B irradiation of different flowering mutants, we gained insight into the pathways involved in the observed flowering time delay in UV-B-exposed Arabidopsis plants. We provide evidence that UV-B light downregulates the expression of MSI1 and CLF, two of the components of the polycomb repressive complex 2, which in consequence drives a decrease in H3K27me3 histone methylation of MIR156 and FLC genes. Modification in the expression of several flowering time genes as a consequence of the decrease in the polycomb repressive complex 2 activity was also determined. UV-B exposure of flowering mutants supports the involvement of this complex in the observed delay in flowering time, mostly through the age pathway.

A responsible fiscal system for Latin America is possible: Dogmatic bases and basic premises constitutive

CONICET Digital -

A responsible fiscal system for Latin America is possible: Dogmatic bases and basic premises constitutive Rezzoagli, Luciano Carlos Effective government action to alleviate social inequalities and boost economic growth, from the strict tax orbit, is a task consciously rejected by theparliament and government. Indeed, they subordinate inclusive public policies, almost exclusively, from "non-commercial" forms, such as those offered by the financial sphere through the use of conditional cash transfers, socialexpenditures and subsidies, tax collection products, others Government revenues and indebtedness. Although in Latin American countries poverty can be temporarily reduce through these government instruments, economic inequality will be maintained or will continue to increase, as will the opportunity grabbing, until there is no structural changes, primarily in the tax field, along with many other integrated solutions beyond the object of our study.

Pautas críticas de la relación existente entre los programas de TMC y los impuestos regresivos al consumo en Argentina

CONICET Digital -

Pautas críticas de la relación existente entre los programas de TMC y los impuestos regresivos al consumo en Argentina Rezzoagli, Luciano Carlos; Villalba, Andres; Perini, Agustina A pesar de la implementación de diversos Programas de TMC y la consecuente, aunque discutida por su escaso nivel de evaluación y control reducción de los índices de pobreza e indigencia y aumento efectivo de inserción laboral de sectores poblacionales vulnerables, Argentina continúa conservando altos niveles de desigualdad. Desde una vertiente de análisis financista, esta situación se debe --en gran medida-- a la consolidación de un sistema detractivo altamente regresivo, caracterizado por la imposición de tributos indirectos predominantes que gravan actividades productivas, intermedias y productos finales de consumo sin tener en cuenta la capacidad contributiva efectiva de los destinatarios reales o incidentales del tributo. En un país como Argentina, perteneciente al continente más desigual del mundo, es indispensable empezar a ser, y no parecer: la estructuración del sistema fiscal debe perseguir y alentar la consecución de una clase media estructural, tomando como punto de partida un contexto de agudas asimetrías económicas tanto a nivel poblacional como a nivel geográfico regional. Una de las principales falencias que consolidan estas situaciones que sufren innumerables familias es la falta de articulación de reformas fiscales que tiendan al desarrollo y consolidación de una clase media estructural mediante un sistema impositivo progresivo, inclusivo y responsable.

El programa de liberación nacional en la Argentina de los 70: La convergencia entre Montoneros y la Confederación General Económica (CGE)

CONICET Digital -

El programa de liberación nacional en la Argentina de los 70: La convergencia entre Montoneros y la Confederación General Económica (CGE); The program of national liberation in the 70s Argentina: from Montoneros to the Confederación General Económica (CGE) Sanz Cerbino, Gonzalo Sebastian; Lissandrello, Guido Axel En este artículo realizamos un estudio del programa político de dos importantes actores del proceso político de la década del ’70 en Argentina: la organización político-militar Montoneros y la corporación empresaria Confederación General Económica (CGE). Mediante el análisis comparado de los documentos fundamentales de ambos, buscamos detectar aspectos comunes y elementos distintivos entre uno y otro. Concluimos que desarrollan variantes de un mismo programa que se plantea el fin del dominio imperialista y la defensa de los intereses nacionales, mediante el estímulo a la empresa nacional y la redistribución del ingreso: el programa de liberación nacional.; In this article we conducted a study of the political program of two major players in the political process in the 70s in Argentina: the military-political organization Montoneros and the interest group Confederación General Económica (CGE). Through a comparative analysis of the fundamental documents of both organizations, we seek to detect coincidences and distinctive elements of each other. We conclude that they develop variants of the same program which propose the end of imperialist domination and defense of national interests, by encouraging national company and income redistribution: the program of national liberation.

Los industriales automotores y la crisis de la década de 1980 en Argentina

CONICET Digital -

Los industriales automotores y la crisis de la década de 1980 en Argentina; Automotive entrepreneurs and the crisis of the 1980s in Argentina Bil, Damián Andrés Analizamos aquí los elementos de la crisis automotriz iniciada en 1978, las condiciones del mercado y la situación de las terminales en la década de 1980. Luego, nos concentramos en el estudio de las posiciones de los empresarios, tanto en términos individuales como actuando en forma colectiva frente a la recesión. Con esto, buscamos aportar al conocimiento sobre las dificultades de la industria argentina en los 80 y sobre la historia de la burguesía local en este período. Utilizamos informes oficiales y privados, series oficiales, publicaciones periódicas y aportes de otros autores.; We analyze the elements of the automotive crisis initiated in 1978, and the market conditions and te situation of the terminals in the 1980s. Then, we focus on the study of the positions of businessmen, both individually and collectively at the time of economic recession. We seek to contribute to the knowledge about the difficulties of the Argentine industry in the ‘80s and the history of the local bourgeoisie in this period. We use official and private reports, journals of the period and contributions from other authors.

A comparative study of the gastric ossicles of Trichodactylidae crabs (Brachyura: Decapoda) with comments on the role of diet and phylogeny in shaping morphological traits

CONICET Digital -

A comparative study of the gastric ossicles of Trichodactylidae crabs (Brachyura: Decapoda) with comments on the role of diet and phylogeny in shaping morphological traits de Azevedo Carvalho, Débora; Collins, Pablo Agustin; Lima Gomes, Renata; Magalhaes Filho, Celio Ubirajara; Torres, María Victoria; Williner, Verónica The gastric armature of decapod foregut is a feeding structure that sparks controversial debates about the role dietary and historical components have in shaping its morphological traits. Having previous information about the natural diet is an interesting way to gather evidence on this issue. For the present study, we analyzed the morphological traits of gastric ossicles involved directly in the maceration of food in nine species of freshwater crabs of the family Trichodactylidae (Brachyura: Decapoda) representing five genera, three tribes and two subfamilies. The analyzed gastric ossicles were quite consistent among closely related species, suggesting that the observed traits had a clear phylogenetic component. However, it was also noted that the morphological traits of the gastric teeth of trichodactylid crabs match well with the natural diet and presented likeness with general features of other species with a similar trophic habit. We discuss the influence of phylogeny and function on the design of morphological traits and propose to quantify the role of phylogeny and function in shaping morphological traits through the analysis of phylogenetic signals.

Development of 3D printed fibrillar collagen scaffold for tissue engineering

CONICET Digital -

Development of 3D printed fibrillar collagen scaffold for tissue engineering Díaz Nocera, Aden; Comin, Romina; Salvatierra, Nancy Alicia; Cid, Mariana Paula Collagen is widely used in tissue engineering because it can be extracted in large quantities, and has excellent biocompatibility, good biodegradability, and weak antigenicity. In the present study, we isolated printable collagen from bovine Achilles tendon and examined the purity of the isolated collagen using sodium dodecyl sulfate polyacrylamide gel electrophoresis. The bands obtained corresponded to α 1 , α 2 and β chains with little contamination from other small proteins. Furthermore, rheological measurements of collagen dispersions (60 mg per ml of PBS) at pH 7 revealed values of viscosity of 35.62 ± 1.42 Pa s at shear rate of 10 s − 1 and a shear thinning behavior. Collagen gels and solutions can be used for building scaffolds by three-dimensional (3D) printing. After designing and fabricating a low-cost 3D printer we assayed the collagen printing and obtaining 3D printed scaffolds of collagen at pH 7. The porosity of the scaffold was 90.22% ± 0.88% and the swelling ratio was 1437% ± 146%. The microstructure of the scaffolds was studied using scanning electron microscopy, and a porous mesh of fibrillar collagen was observed. In addition, the 3D printed collagen scaffold was not cytotoxic with cell viability higher than 70% using Vero and NIH 3 T3 cells. In vitro evaluation using both cells lines demonstrated that the collagen scaffolds had the ability to support cell attachment and proliferation. Also a fibrillar collagen mesh was observed after two weeks of culture at 37 °C. Overall, these results are promising since they show the capability of the presented protocol to obtain printable fibrillar collagen at pH 7 and the potential of the printing technique for building low-cost biocompatible 3D plotted structures which maintained the fibrillar collagen structure after incubation in culture media without using additional strategies as crosslinking.

Density of spaces of trigonometric polynomials with frequencies from a subgroup in L∝-spaces

CONICET Digital -

Density of spaces of trigonometric polynomials with frequencies from a subgroup in L∝-spaces; Les espaces de polynômes trigonométriques avec fréquences dans un sous-groupe sont denses dans les espaces Lα Medina, Juan Miguel; Klotz, Lutz Peter; Riedel, Manfred Let G be an LCA group, H a closed subgroup, Γ the dual group of G and μ be a regular finite non-negative Borel measure on Γ. We give some necessary and sufficient conditions for the density of the set of trigonometric polynomials on Γ with frequencies from H in the space Lα(μ),α∈(0,∞).; Soit G un groupe abélien, localement compact pour une topologie séparée, H un sous-groupe fermé, Γ le groupe dual de G et μ une mesure de Borel positive ou nulle, régulière et finie sur Γ. Nous donnons des conditions nécessaires et suffisantes pour que l'ensemble des polynômes trigonométriques sur Γ avec fréquences dans H soit dense dans Lα(μ),α∈(0,∞).

An environmental magnetism approach to assess impacts of land-derived sediment disturbances on coral reef ecosystems (Cartagena, Colombia)

CONICET Digital -

An environmental magnetism approach to assess impacts of land-derived sediment disturbances on coral reef ecosystems (Cartagena, Colombia) Mejia Echeverry, Daniela; Chaparro, Marcos Adrián Eduardo; Duque Trujillo, Jose Fernando; Restrepo, Juan D. We used environmental magnetism methods to study recently deposited marine sediments from the estuarine ecosystems on the Caribbean coast of Colombia. Cartagena region has undergone an increasing sediment load during the last decades via sediment plumes from Magdalena River and its distributary man-made channel. Concentration dependent magnetic parameters show an increasing abundance of ferrimagnetic minerals on the uppermost sediments on sites located close to the continent (remanent magnetization SIRM = 5.4-9.5 × 10−3Am2 kg−1) as well as faraway sites (SIRM = 0.5-1.7 × 10−3Am2 kg−1 near Rosario Islands coral reef complex). The magnetic grain size and mineralogy along the cores are variable, showing the dominance of the magnetite-like minerals (remanent coercivity Hcr = 34.3-45.3 mT), with a minor contribution of high-coercivity minerals (Hcr = 472-588 mT). In addition, there is a moderate enrichment of elements Cu, Mo, and Zn (enrichment factor EF = 1.5-3.8) that indicates the additional land-derived contribution on sediments. The environmental magnetism approach, which shows significant signals of magnetic minerals and trace elements, is a reliable tool to prove the presence of continental sediment supply in coral reef ecosystems.

Comprehensive transcriptomic analysis of heat shock proteins in the molecular subtypes of human breast cancer

CONICET Digital -

Comprehensive transcriptomic analysis of heat shock proteins in the molecular subtypes of human breast cancer Zoppino, Felipe Carlos Martin; Guerrero Gimenez, Martin Eduardo; Castro, Gisela Natalia; Ciocca, Daniel Ramon Background: Heat Shock Proteins (HSPs), a family of genes with key roles in proteostasis, have been extensively associated with cancer behaviour. However, the HSP family is quite large and many of its members have not been investigated in breast cancer (BRCA), particularly in relation with the current molecular BRCA classification. In this work, we performed a comprehensive transcriptomic study of the HSP gene family in BRCA patients from both The Cancer Genome Atlas (TCGA) and the Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) cohorts discriminating the BRCA intrinsic molecular subtypes. Methods: We examined gene expression levels of 1097 BRCA tissue samples retrieved from TCGA and 1981 samples of METABRIC, focusing mainly on the HSP family (95 genes). Data were stratified according to the PAM50 gene expression (Luminal A, Luminal B, HER2, Basal, and Normal-like). Transcriptomic analyses include several statistical approaches: differential gene expression, hierarchical clustering and survival analysis. Results: Of the 20,531 analysed genes we found that in BRCA almost 30% presented deregulated expression (19% upregulated and 10% downregulated), while of the HSP family 25% appeared deregulated (14% upregulated and 11% downregulated) (|fold change| > 2 comparing BRCA with normal breast tissues). The study revealed the existence of shared HSP genes deregulated in all subtypes of BRCA while other HSPs were deregulated in specific subtypes. Many members of the Chaperonin subfamily were found upregulated while three members (BBS10, BBS12 and CCTB6) were found downregulated. HSPC subfamily had moderate increments of transcripts levels. Various genes of the HSP70 subfamily were upregulated; meanwhile, HSPA12A and HSPA12B appeared strongly downregulated. The strongest downregulation was observed in several HSPB members except for HSPB1. DNAJ members showed heterogeneous expression pattern. We found that 23 HSP genes correlated with overall survival and three HSP-based transcriptional profiles with impact on disease outcome were recognized. Conclusions: We identified shared and specific HSP genes deregulated in BRCA subtypes. This study allowed the recognition of HSP genes not previously associated with BRCA and/or any cancer type, and the identification of three clinically relevant clusters based on HSPs expression patterns with influence on overall survival.

Páginas

Suscribirse a Facultad de Ciencias Químicas de la Universidad Nacional de Córdoba agregador