Facetas de la vulnerabilidad sociodemográfica de la provincia de Misiones (Argentina).; Aspects of the sociodemographic vulnerability of the Province of Misiones (Argentina) Foschiatti, Ana Maria Hilda Geográicamente, el objetivo de este trabajo es describir e interpretar el comportamiento de la distribución de la población y su inluencia en la vulne-rabilidad y en la pobreza de los hogares y de los habitantes de una provincia argentina de frontera. Se consideran las Necesidades Básicas Insatisfechas, el hacinamiento, los problemas del hábitat y las viviendas, los servicios públi-cos y algunas repercusiones en la educación y la protección sanitaria; From the geographical point of view, the purpose of this paper is to de-scribe and interpret the population distribution behaviour, and its inluence on the vulnerability and the poverty in home life and upon the inhabitants of an Argentine frontier province. The Unsatisied Basic Needs, overcrowded hous-ing, habitat and dwelling problems, public services and some of their effects on education and health care are taken into account

First genotoxicity study of Paraná River water from Argentina using cells from the clam Corbicula fluminea (veneroida corbiculidae) and Chinese hamster (Cricetulus griseus Rodentia, Cricetidae) K1 cells in the comet assay Caffetti, Jacqueline Diana; Mantovani, Mário S.; Pastori, María Cristina; Fernocchio, Alberto S. High concentrations of xenobiotics from urban and industrial wastes have contributed to the contamination of many aquatic environments. We used the comet assay to evaluate the genotoxic potential of water collected from the River Paraná, which receives a great deal of waste, at three points (Puerto Piray, Eldorado and Montecarlo) in the Misiones Province of Argentina. The in vivo comet assay used 40 freshwater clams (Corbicula fluminea) while the in vitro comet assay used Chinese hamster (Cricetulus griseus) K1 cell (CHO-K1) cultures with the mutagen ethyl methanesulfonate (EMS) as the positive control and phosphate buffered saline (PBS) as the negative control. Both assays showed statistically significant differences between the three sampling sites in relation to the negative control, the results of this preliminary study indicating that at these three sites water from the Paraná River presents genotoxic potential. Copyright © 2008, Sociedade Brasileira de Genética.

Singlet oxygen quenching by anthocyanin's flavylium cations Rosso, Veridiana V. De; Moran Vieyra, Faustino Eduardo; Mercadante, Adriana Z.; Borsarelli, Claudio Darío The quenching of singlet molecular oxygen (1O2) by the flavylium cation form of six widespread anthocyanin derivatives: cyanidin 3-glucoside (CG), cyanidin 3-rutinoside (CR), cyanidin 3-galactoside (CGL), malvidin (M), malvidin 3-glucoside (MG) and malvidin 3,5-diglucoside (MDG) was studied in 1% HCl methanol solution by time-resolved phosphorescence detection (TRPD) of 1O2 and photostationary actinometry using perinaphthenone and methylene blue as sensitizers, respectively. The average value of the total (k0) and chemical (kc) quenching rate constants were ∼ 4×108 M-1 S-1 and 3×106 M-1 S-1, respectively, indicating the good performance of the studied anthocyanins as catalytic quenchers of 1O2. The quenching efficiency was larger for malvidin than for cyanidin derivatives, probably by the extra electron-donating methoxy group in ring B of the malvidin derivatives; and it was also dependent on the number and type of glycosylated substitution. As observed for other phenolic-like derivatives, the quenching of 1O2 by anthocyanins was mediated by a charge-transfer mechanism, which was modulated by the total number of -OR substituents that increases the electron-donating ability of these compounds.

De obligaciones y derechos en coro y altar. La obra de Juan Gil Trullench Martinez, Ana Maria; Consigli, Julieta M. La creación de diócesis fue uno de los modos que tuvo la monarquía y el pasado para organizar jurisdiccionalmente en Indias. La constitución de los cabildos catedralicios, con sus dignidades, prebendados, raciones y canonjías, además de los oficios, permitieron que funcionara el Coro y el Altar como lo requería del Oficio Divino y la celebración de las misas. Tanto el cumplimiento de las obligaciones que ellos asumían como los derechos que tales cargos conllevaban, como por ejemplo los emolumentos que percibían por estar y participar del Coro y el Altar , fueron materia de normas canónicas y reales y produjeron una abundante doctrina. La proliferación de obras que abordaron ese tema, como de otras que los llevaban inserto en algún capítulo, llevó al teólogo valenciano Juan Gil Trullench a escribir un opúsculo que contuviera todas aquellas cosas que era necesario que supieran para no alegar ignorancia al respecto. A esta intención responde De obligatione assistendi, et canendi in Choro, publicado en Valencia en 1633, obra en la que recogió lo que habían sostenido en distintas ocasiones "reconocidos doctores", sumado a su propio parecer.El trabajo que se presenta contiene la contextualización del tema, el autor y la obra e incluye la traducción del Prefacio, como una primera etapa del procesamiento completo de la obra.

El comercio de la provincia de Corrientes durante la primera mitad del siglo XIX. Un panorama de su evolución Schaller, Enrique Cesar El presente trabajo pretende contribuir al mejor conocimiento de los circuitos mercantiles en la Argentina durante la etapa previa a la organización nacional. Con ese fin se analiza la evolución general del comercio exterior de Corrientes durante la primera mitad del siglo XIX. En ese lapso la economía de esa provincia experimentó grandes fluctuaciones. Tras el considerable crecimiento productivo y comercial durante el siglo XVIII una fuerte crisis afectó al sector ganadero durante la etapa revolucionaria. El nuevo equilibrio logrado en la década de 1820 pareció abrir nuevas posibilidades, sin embargo, en el desenvolvimiento de la economía rioplatense Corrientes se vio relegada a un papel cada vez más marginal sobre todo a partir del largo conflicto contra Rosas en la década de 1840.. Este estudio se basa en la documentación de fiscal de la provincia. En primer lugar se ha tratado de establecer el valor monetario (en pesos plata) de las exportaciones e importaciones. A partir de los montos establecidos se busca reconstruir la evolución general de este tráfico, examinar la importancia de las vinculaciones comerciales de la provincia y la composición de su flujo exportador.; This paper aims to contribute to the better knowledge of commercial circuits in Argentina before the National Organization. It analyzes the evolution of Corrientes’ overall foreign trade during the first half of the nineteenth century. During that time, the economy of the province experienced large fluctuations. Following the considerable growth of production and trade in the eighteenth century, a strong crisis affected the livestock sector during the revolutionary period. The new stability achieved in the 1820 decade seemed to open new possibilities; however, in the economic growth of the Río de la Plata region, Corrientes was relegated to an increasing marginal role, particularly since the extended conflict against Rosas in the decade of 1840. The study is based on fiscal documentation. First, it has tried to establish the monetary value (in pesos silver) of exports and imports, and then, to rebuild the overall evolution of this exchange traffic, considering the importance of the province external commercial linkages and the composition of its export flow.

Guías del Chaco y de Formosa. Útiles informaciones del pasado, valiosas fuentes para investigaciones actuales Beck, Hugo Humberto En la primera mitad del siglo XX se editaron en Formosa y en Chaco diversas Guías con información destinada a productores, a comerciantes, a profesionales y a todo público. Sus datos fueron de suma utilidad para los habitantes de una región con escaso acceso a medios de difusión masiva. Actualmente constituyen una fuente de enorme valor para la reconstrucción del pasado de las actuales provincias del Chaco y de Formosa. El trabajo describe la información reunida en esas publicaciones, las ordena temáticamente y pondera su valor.

Expression of epithelial cadherin in the human male reproductive tract and gametes and evidence of its participation in fertilization Marin Briggiler, Clara Isabel; Veiga, Maria Florencia; Matos, María Laura; González Echeverría, M. F.; Furlong, L. I.; Vazquez, Monica Hebe Epithelial cadherin (E-cadherin) has been involved in several calcium-dependent cell-cell adhesion events; however, its participation in gamete interaction has not been fully investigated. Our results have demonstrated expression of E-cadherin mRNA in the human male reproductive tract showing higher levels in the caput, corpus and cauda epididymis than in the testis. The mature 122 kDa E-cadherin was detected in epididymal protein extracts and was localized in the epithelial cells from the three epididymal regions. Moreover, the 86 kDa E-cadherin ectodomain was found in cauda epididymal and seminal plasma. Western immunoblotting of human sperm protein extracts allowed the identification of four E-cadherin forms (122, 105, 97 and 86 kDa). The protein was localized in the acrosomal region of intact spermatozoa, remained associated with the head of acrosome-reacted cells and was also detected on the oocyte surface. A similar localization was determined for other proteins of the adhesion complex (beta-catenin and actin). Spermatozoa incubated with anti-E-cadherin antibodies showed impaired binding to homologous zona pellucida (ZP); in addition, presence of these antibodies inhibited the penetration of human spermatozoa to ZP-free hamster oocytes. The results presented here describe the expression of E-cadherin in the male reproductive tract and gametes and strongly suggest its involvement in adhesion events during human fertilization. The identification of proteins involved in gamete interaction will contribute to the understanding of the molecular basis of fertilization and help in the diagnosis and treatment of infertility.

Adquisición de significados verbales nuevos por un proceso de asociación multimodal Fernández Feijóo, María Eugenia; Vernis, Sergio; Zanutto, Bonifacio Silvano; Wainselboim, Alejandro Javier Mientras que la adquisición del lenguaje durante la infancia se produce por exposición al contexto lingüístico, en adultos esta capacidad no parecería estar presente, pues la adquisición de las reglas gramaticales de una lengua requiere de procesos de aprendizaje explícitos (concientes). Se ha visto que infantes de 4 a 8 meses pueden asociar información visual con información auditiva de tipo lingüística con breves tiempos de presentación. La mayor dificultad en adultos por adquirir una segunda lengua por simple exposición al contexto lingüístico podría quizás en parte deberse a una dificultad en asociar entradas sensoriales múltiples con el estímulo lingüístico adecuado. Se estudió entonces qué capacidades poseen los adultos de asociar significados a formas verbales nuevas por co-ocurrencia entre acciones y formas lexicales nuevas. Se presentaron como entrenamiento 60 escenas visuales diferentes junto con frases formadas por no palabras que describían las escenas mostradas. Durante el testeo se presentaron 40 escenas nuevas junto con frases nuevas. En el 50% de los casos la frase describía correctamente la escena mostrada (frase Correcta), mientras que en el restante 50% el “verbo” no coincidía con la acción mostrada (frase Incorrecta). Los participantes decidían lo más rápidamente posible si la frase describía correctamente la escena o no. Los resultados mostraron que en adultos es posible adquirir significados verbales nuevos por co-ocurrencia entre acciones (movimientos) y formas lexicales nuevas. Diferencias de respuesta halladas entre tipos de frase durante el testeo permiten especular con respecto a los mecanismos involucrados en el proceso de decisión durante esta fase.; Infant language acquisition develops by simple exposure to the linguistic context, while in adults acquisition of the grammatical rules of a language requires explicit (i.e. conscious) learning processes. It has been shown that 4 to 8 month old infants can associate visual information with auditive information that has a linguistic form with only brief exposure times. The greater difficulty in adults in acquiring a second language by simple exposure may be in part due to difficulties in associating multiple sensory inputs with the adequate linguistic stimulus. We studied therefore the capacity in adults to assign meaning to new verbal forms by co-ocurrence between actions and new lexical labels. During training 60 different visual scenes were presented along with sentences formed by non-words that described the presented scenes. During the testing phase 40 new scenes were presented together with new sentences. In 50% of the cases the sentence described correctly the scene (Correct sentence), while in the remaining 50% the “verb” did not agree with the shown action (Incorrect sentence). Participants had to decide as fast and accurately as possible whether each sentence correctly described each visual scene. Results show that adults can assign new verbal meanings by co-occurrence between actions (movements) and new lexical labels. Differences in response patterns between both types of sentences during testing allow speculating about the mechanisms involved during the decision process in this phase.

Large neutral fractions in collisions of Li+ with a highly oriented pyrolytic graphite surface:Resonant and Auger mechanisms Bonetto, Fernando Jose; Romero, Marcelo Ariel; García, Evelina Andrea; Vidal, Ricardo Alberto; Ferron, Julio; Goldberg, Edith Catalina We report measurements of neutral atom fractions for Li+ scattered by a highly oriented pyrolytic graphite surface as a function of the incident ion energy and exit angle. We found an unexpected large neutralization probability that increases with both energy and exit angle. Based on a dynamical quantum calculation of the scattering process that accounts for the extended features of the surface and the localized nature of the atom-atom interactions, we propose that the resonant neutralization to the Li ground state is a relevant mechanism in this collision. However, to understand the whole process including the angular dependence, and taking into account the Li energy-level variations, we conclude that other mechanisms such as Auger neutralization to the ground state and resonant neutralization to excited states should also be considered. 

Reaction kinetics of bacteria disinfection employing hydrogen peroxide Labas, Marisol Daniela; Zalazar, Cristina Susana; Brandi, Rodolfo Juan; Cassano, Alberto Enrique The inactivation reaction of Escherichia coli bacteria employing hydrogen peroxide at 20 ºC and pH = 7 was studied in a well-mixed batch reactor. The proposed objective, as far as the extent of inactivation is concerned, was obtained for H2O2 concentrations above 100 ppm (1 ppm = 2.94×10-5 mmol cm-3) but, compared with other disinfection technologies, for too long reaction times. Below 40 ppm of the oxidant concentration inactivation was practically ineffective. Results were analyzed employing Modified forms of the Series-Event and Multitarget mechanistic models. At concentrations above 100 ppm the induction time in the semi-logarithmic plot of bacteria concentration versus time was reduced. With both modified models it was found that the reaction order with respect to the hydrogen peroxide concentration was different than one. Both mathematical descriptions provide a good representation of the experimental results in an ample range of the disinfectant concentrations and confirm a methodology that renders the starting point of a reaction kinetic expression useful for further studies regarding the optimization of the operating conditions (pH and temperature, for example), including also combination with other advanced oxidation technologies. An interpretation of the data in terms of a Weibull-like model [1] is also included.

Hydration, charge, size, and shape characteristics of peptides from their CZE analyses Peirotti, Marta Beatriz; Piaggio, María Virginia; Deiber, Julio Alcides A capillary zone electrophoresis model is presented for peptide characterization, on the base of well established physicochemical equations.The effective mobility is used as basic data in the model to estimate relevant peptide properties like, for instance, hydration, net and total electrical charge numbers, hydrodynamic size and shape, particle average orientation, and pH-microenvironment from the charge regulation phenomenon. Therefore 102 experimental effective mobilities of different peptides are studied and discussed in relation to previous works. An equation for the estimation of peptide hydration as a function of ionizing, polar and non polar amino acid residues is included in the model. It is also shown that the shape-orientation factor of peptides may be either lower or higher than one, and its value depends on a complex interplay among mainly charge number, molar mass, hydration and amino acid sequence.

In,Fe-Zeolites: active and stable catalysts for the SCR of NOx. Kinetics, characterization and deactivation studies Serra, Ramiro Marcelo; Vecchietti, María Julia; Miro, Eduardo Ernesto; Boix, Alicia Viviana In this work, the addition of Fe2O3 to InH-zeolites (ZSM5 and mordenite) is investigated for the selective catalytic reduction of NOx with both methane and butane as reducing agents. When Fe is added to InHMOR and methane is used, the NOx to N2 conversion increases from 25% to 55% at 20,000 h-1 and 500 8C. An increase is also observed when butane is employed but to a lower extent. The role of Fe could be ascribed to an improvement of the NO to NO2 reaction rate. The presence of iron oxides also improves durability in the presence of 2% water. The catalyst InFeHMOR undergoes a fast initial inhibition after which a reasonable NOx conversion (25%) remains constant during 125 h. TPR, XRD and LRS characterizations indicate that Fe2O3 and Fe3O4 are the iron species present, while InO+ at exchange sites and small amounts of In2O3 are also present. The kinetic plots for the NOx selective catalytic reduction (SCR) with methane with the InFeHMOR catalyst show fractional orders for the NO concentration and ca. first order for methane.

High-energy emission from accreting black holes with high-mass donor stars Vila, Gabriela Soledad; Romero, Gustavo Esteban We present an analysis of the interactions of a distribution of relativistic protons under the typical conditions of the corona surrounding an accreting black hole. The model is applied to a concrete binary system with a high-mass donor star: the classical source Cygnus X-1

HDE 269896: una supergigante especial de la nube mayor de Magallanes Corti, Mariela Alejandra; Walborn, Nolan Revere The star HDE 269896, a hypergiant in the Large Magellanic Cloud, with spectral type ON9.7 Ia+, is a special case within the known OB Zoo. We have discovered strong emission from the N II 5000 ˚A complex in its spectrum, and the previously known He II λ4686 emission, with an anomalous strength for the spectral type, has also been observed. Comparing HDE 269896 with other objects that show N II emission lines, such as WN10 and WN11 types, we find that this star is highly evolved, albeit less so than the latter stars. Thus, it is reasonable to propose that HDE 269896 should be in an immediate pre-WNVL (Very Late WN) state. Another characteristic of this star is the unusually wide range of ionization present in its extended atmosphere. Subsequent analysis of its spectrum will permit a better understanding of both the relevant atomic physics and the structure of this unusual atmosphere.; La estrella HDE 269896, hipergigante de la Nube Mayor de Magallanes, con tipo espectral ON9.7 Ia+, es un caso particular dentro del zoológico OB conocido. Hemos encontrado en su espectro, una intensa emisión del complejo N II 5000 A y se ha observado también una emisión ˚ de He II λ4686, la cual por su intensidad, resulta anómala para su tipo espectral. Comparando a HDE 269896 con otros objetos que muestran líneas de emisión de N II, como ser WN10 y WN11, resulta evidente que HDE 269896 es un objeto altamente evolucionado, aunque lo es menos que las estrellas WN10 y WN11. Por esto, resulta razonable proponer que HDE 269896 debe hallarse en un estado inmediato de pre-WNVL (Very Late WN). Otra particularidad presente en HDE 269896, es el inusualmente amplio rango de ionización que tiene lugar en su atmósfera extendida. Análisis posteriores de su espectro permitirán comprender mejor tanto la física atómica involucrada como la estructura de esta atmósfera inusual.

La génesis de los Universales en Prasastapada y la génesis de los Principia Prima y los Universales en Aristóteles Tola, Fernando; Dragonetti, Carmen Leonor En este artículo los autores ofrecen un nuevo caso en soporte de su tesis concerniente a las frecuentes coincidencias temáticas en las Filosofías de la India y de Occidente, que ellos han desarrollado en especial en sus libros On the Myth of the Opposition between Indian Thought and Western Philosophy, Hildesheim: Olms Verlag, 2004, reseñado por Ernst Steinkellner en Wiener Zeitschrift für die Kunde Südasiens Band XLVIII 2004, 224-225, y Yoga. Un Camino Místico Universal, Barcelona: Editorial Kairós, 2006. Dicha tesis apunta a lo que podríamos llamar una suerte de globalidad intrínseca del pensar filosófico, más allá de la cultura de que se trate. Praastapda (circa segunda mitad del siglo VII d.C.), en Praastapdabh=ya (llamado también Padrthadharmas{graha), Libro V: Smnyapadrthanirpaa, da dos descripciones de la génesis de los universales. Describe primeramente de una manera abstracta y concisa el proceso de surgimiento de todo smnya, “universal”, y después explica de una manera más concreta el surgimiento del universal inferior y del superior (satt, “la existencia”), a través de la reiteración de la percepción de objetos que presentan similares atributos y de la intervención del recuerdo de percepciones similares pasadas. Aristóteles (siglo IV a.C.), en Analytica Posteriora, Libro II, Capítulo XIX, y Temistio (circa 317-circa 387), en su comentario, explican a su vez el modo en que son aprehendidos los principios universales o premisas de la demostración (principia prima). Los autores de este artículo muestran cómo la descripción de Aristóteles y Temistio de la génesis de los principia prima y los universales concuerda de manera sorprendente con la de Praastapda concerniente a los universales.

Effect of iron on the properties of sulfated zirconia Costa Pereira, Amalia Luz; Marchetti, Sergio Gustavo; Albornoz, Alberto; Reyes , Patricio; Oportus, Marcelo; Rangel. Maria do Carmo Sulfated zirconia has found several industrial applications, mainly for catalyzing reactions which require acidic sites. In order to control the properties of this solid, the effect of iron on the sulfated zirconia properties was studied in this work. Samples with different iron to zirconium molar ratios were prepared by the sol–gel method, followed by sulfation and characterization by several techniques. It was found that all iron ions go into zirconia when Fe/Zr 0.4. However, when higher amounts of iron (Fe/Zr 0.8) are added, hematite is segregated. Different phases can be obtained depending on the iron content and on the sulfur presence. Iron increases the specific surface areas of both sulfated and pure zirconia but does not affect the incorporation of sulfate groups as compared to pure zirconia. Iron also makes the solids thermally more stable and modifies the sulfated zirconia surface creating acidic sites with medium strength. Therefore, it is possible to control the acidity of the solid doping the sulfated zirconia with iron. All these changes are very useful for catalytic applications.

Q-spaces and the foundations of quantum mechanics Domenech, Graciela; Holik, Federico Hernan; Krause, Décio Our aim in this paper is to take quite seriously Heinz Post’s claim that the non-individuality and the indiscernibility of quantum objects should be introduced right at the start, and not made a posteriori by introducing symmetry conditions. Using a different mathematical framework, namely, quasi-set theory, we avoid working within a labeltensor-product-vector-space-formalism, to use Redhead and Teller’s words, and get a more intuitive way of dealing with the formalism of quantum mechanics, although the underlying logic should be modified. Thus, this paper can be regarded as a tentative to follow and enlarge Heinsenberg’s suggestion that new phenomena require the formation of a new “closed” (that is, axiomatic) theory, coping also with the physical theory’s underlying logic and mathematics.

A topological study of contextuality and modality in quantum mechanics Domenech, Graciela; Freytes Solari, Hector Carlos; de Ronde, Christian Kochen-Specker theorem rules out the non-contextual assignment of values to physical magnitudes. Here we enrich the usual orthomodular structure of quantum mechanical propositions with modal operators. This enlargement allows to refer consistently to actual and possible properties of the system. By means of a topological argument, more precisely in terms of the existence of sections of sheaves, we give an extended version of Kochen-Specker theorem over this new structure. This allows us to prove that contextuality remains a central feature even in the enriched propositional system.

Larnax altomayoense y Larnax chotanae (Solanaceae) dos nuevas especies de los bosques montanos del Norte del Perú; Larnax altomayoense y Larnax chotanae (Solanaceae) two new species from montane forests of Northern Peru Leiva González, Segundo; Pereyra Villanueva, Erwin; Barboza, Gloria Estela Se describen Larnax altomayoense y Larnax chotanae (Solanaceae) dos nuevas especies de los Bosques Montanos del Norte del Perú. Larnax altomayoense S. Leiva & Quipuscoa propia del Bosque de Protección del Alto Mayo, ruta entre el poblado de Aguas Verdes-Santa Rosa del Alto Mayo, Prov. Rioja, Dpto. San Martín, Perú, entre los 980-1010 m de elevación, se caracteriza por las flores 1-2 por nudo, cáliz rodeado por una cobertura de pelos glandulares transparentes externa e interiormente, corola campanuladaestrellada, glabra externa e interiormente, lóbulos corolinos lila-intenso cerca del 90% del área central con los bordes verdoamarillentos, lustrosos interiormente, filamentos estaminales homodínamos, cremosos, anteras morado-intenso, ovario con algunas pintas lilas longitudinales en la mitad distal, estilo exerto, baya cónica, semillas 88-95 por baya, arbustos de (0,70-) 1,20-1,50 m de alto y Larnax chotanae S. Leiva, Pereyra & Barboza habita en el bosque El Pargo (ruta Llama-Huambos), Prov. Chota, Dpto. Cajamarca, Perú, alrededor de los 3050 m de elevación, presenta las flores (2-) 3-4 por nudo, corola campanulada-estrellada, amarillo externamente, verdoso interiormente, limbo de 12-14 mm de diámetro en la antésis, estambres exertos, filamentos estaminales homodínamos, amarillos, anteras moradas, estilo exerto, amarillento, baya cónica amarillento-anaranjada, semillas 22-49 por baya, esclerosomas 12- 14 por baya, arbustos de 1,20-1,50 m de alto. Adicionalmente a las descripciones se presentan las ilustraciones correspondientes, se discuten sus relaciones con otras especies afines e incluyen datos sobre su distribución geográfica, ecología y situación actual.

Analysis of sequence configurations of the PKR-interacting HCV proteins from plasma and PBMC as predictors of response to interferon-alpha and ribavirin therapy in HIV-coinfected patients Bolcic, Federico Martin; Bull, L.; Martinez, L.; Reynoso, Rita Paola; Salomon, H.; Arduino, R.; Barnett, B.; Quarleri, Jorge Fabian Antecedentes: El tratamiento basado en interferon es una terapia estándar contra la hepatitis C crónica en pacientes coinfectados con el VIH. Exploramos el rol de dos regiones genómicas del HCV en la respuesta al IFN entre los pacientes coinfectados HIV-HCV. A través de una cohorte homogénea retrospectiva, evaluamos la frecuencia de las mutaciones E2 y NS5A en pacientes con y sin respuesta viral sostenia a la terapia basada en IFN. Métodos: Se individualizaron restrospectivamente las muestras de una cohorte homogenea de 10 pacientes coinfectados con HCV-VIH que completaron la terapia con IFN. Todos los pacientes estaban bajo tratamiento con la terapia HAART. El genotipo del HCV (basado en 5?NCR+NS5B relacionado filogenéticamente) y los análisis genómicos E2/NS5A se realizaron en compartimentos con muestras de suero y PBMC. Se analizaron las mutaciones carboxiterminales de E2, incluyendo el dominio homólogo de fosforilación PKR/eIF2alfa (PePhD) y las mutaciones de dominio unidas a la proteina kinasa de 5A no estructurado (PKRBD). Resultados: Cuatro pacientes lograron una respuesta viral sostenida y 6 fueron no respondedores (NR). Entre los pacientes con respuesta viral sostenida, los genotipos eran Gt-1 (n=3) y Gt4 (n=1).  Los tratamientos fallados fueron infectados con el Gt-1 (n=5) y el Gt-2 (n=1). La distribución genotípica fue igual para ambos grupos, los que obtuvieron respuesta viral sostenida y los no respondedores (p>0.05). Entre los compartimentos, el número de mutaciones en PKRBD fue conservado, mientras que el número de mutaciones de dominio PePHD fueron significativamente menores. Los pacientes NR exhibieron casi el mismo número de mutaciones en ambos compartimentos, mientras que aquellos con respuesta viral sostenida solo demostraron mutaciones en aislados del HCV en plasma. Cuando se realizó una comparación entre pacientes, la distribución de mutación cuantitativa o cualitativa entre los compartimentos de plasma y PBMC de cada región genómica se halló que era casi similar. Conclusiones: La frecuencia de mutaciones en las regiones definidas de la envoltura 2 del HCV PePHD y la proteina 5A no estructurada (NS5A) parece no influenciar la capacidad de IFN-alfa para bloquear la replicación del HCV ni tampoco predecir fehacientemente el resultado del tratamiento en pacientes coinfectados con VIH/HCV.