Ciencia y Tecnología
Construcción de rutinas espaciales y sus efectos en las dinámicas de inclusión-exclusión del activismo LGTB en Córdoba, Argentina; Spatial routines and inclusion/exclusion dynamics among LGBT activists in Córdoba, Argentina; Construção de rotinas espaciais e seus efeitos nas dinâmicas de inclusão-exclusão do ativismo LGTB em Córdoba, Argentina
Rabbia, Hugo Hernán; Iosa, Tomas Abraham
El presente artículo explora la relación entre espacialidad y acción política al interior del movimiento LGTB de Córdoba, Argentina. Se focaliza en la construcción de rutinas espaciales como determinante de la estratificación social en el activismo LGTB. Considerando la trayectoria de los diversos grupos en la militancia "activistas históricos vs. recientes" se indagan usos diferenciales del espacio y sus representaciones, así como sus efectos sobre las dinámicas de inclusión-exclusión en una acción política concreta: la primera marcha local del Orgullo y la Diversidad realizada en noviembre de 2009. Los datos generados mediante registro de observaciones de campo y entrevistas revelan que la emergencia de nuevos grupos de activistas a finales de la presente década implicó un desplazamiento de las rutinas espaciales desde la periferia al centro de la ciudad. Este proceso conlleva dinámicas de inclusión-exclusión que determinan la estructura social del activismo, condicionando el objetivo de lograr una convocatoria integrada por un amplio espectro social de participantes.
Cariotipos de Liolaemus quilmes y Liolaemus wiegmannii y comparación con otros taxones del grupo boulengeri
Cariotipos de Liolaemus quilmes y Liolaemus wiegmannii y comparación con otros taxones del grupo boulengeri; Karyotypes of Liolaemus quilmes and Liolaemus wiegmannii and its comparison with other taxa of the boulengeri group
Aiassa, Delia Elba; Gorla, Nora Bibiana Maria
Se describe el cariotipo de Liolaemus quilmes (2n= 32), que no estaba mostrado previamente, y el de Liolaemus wiegmannii (2n= 32), que difiere de otros informes para la especie obtenidos a partir de animales geográficamente muy distantes de los estudiados en este trabajo. Se analizaron metafases de médula ósea y epitelio intestinal de dos ejemplares de cada especie. Ambas presentan la morfología de seis pares de macrocromosomas metacéntricos (M) y submetacéntricos (SM), el par de microcromosomas número (#)7 con morfología acrocéntrico-telocéntrico (A-T) y los microcromosomas #8 y 9 con morfo- logía M-SM, el resto de los microcromosomas son puntiformes. Los pares #7, 8 y 9 de L. quilmes son en promedio un 21% más grandes que los de L. wiegmannii y en este punto del desarrollo citogenético alcanzado para el género es la úni- ca característica que a nivel de cariotipo permite diferenciar am- bas especies. Las otras especies del grupo boulengeri con estudios citogenéticos efectuados presentan cariotipos con 2n= 28, 32, 34 y 36. Solamente L. riojanus presenta 2n= 32 al igual que L. quilmes y L. wiegmannii, pero con diferencias morfológicas y de longitud cromosómica en los tres primeros pares de microcromosomas (pares #7, 8 y 9). Se destaca la importan-cia de estos tres pares de cromosomas para las diferencias interespecíficas dentro del grupo. Palabras clave: Liolaemus, L. quilmes, L. wiegmannii, grupo boulengeri, cariotipo have 2n= 28, 32, 34 and 36. Only L. riojanus shows 2n= 32 like L. quilmes and L. wiegmannii but with morphologic and chromosome length differences in the first three pairs of microchromosomes # 7, 8 y 9. It is recognize the importance of these pairs in the inter-specific differences among the group.
La Misión Pentecostal Escandinava en el Chaco Argentino: Etapa formativa (1914-1945)
La Misión Pentecostal Escandinava en el Chaco Argentino: Etapa formativa (1914-1945); Scandinavian pentecostal mission in the argentine chaco: The first stage (1914-1945)
Ceriani Cernadas, Cesar Roberto
El trabajo propone una etnografía histórica sobre la misión pentecostal escandinava entre los aborígenes del Chaco Argentino durante su período formativo. Fue el misionero noruego Berger Johnsen (1888-1945) quien se instaló en el pueblo de Embarcación (provincia de Salta) en 1914 con el objetivo de organizar una base misionera para el trabajo evangélico. La indagación pretende sumar conocimiento sobre esta corriente olvidada en los estudios sobre evangelización protestante en la región chaqueña tomando en cuenta cuatro ejes: 1. los valores, el imaginario y las prácticas del misionero nórdico; 2. la recepción aborigen del mensaje pentecostal, observada en los dirigentes y la memoria de los habitantes; 3. la coyuntura regional, religiosa, política y económica, que enmarcó estructuralmente el emprendimiento; 4. la realidad cultural sui generis de la misión de Embarcación, conformada por diversos grupos étnicos del corazón chaqueño y establecida en un naciente pueblo criollo.; This paper proposes an historical ethnography of the Scandinavian Pentecostal mission among the aboriginal people of the Argentinean Chaco focusing on its former period. In 1914 a Norwegian missionary, Berger Johnsen (1888-1945), established a Pentecostal base in Embarcación (Province of Salta) in order to embrace evangelical work. This study tries to add knowledge to this neglected trend in the studies about Protestant evangelism in the Chaco region. This will be done taking into account four topics: 1. the values, representations and practices of the Nordic missionary; 2. the aboriginal appropriation of the Pentecostal message; 3. the regional context, in terms of religion, politics and economy, framing the missionary enterprise; 4. the sui generis cultural reality of Embarcación mission, characterized by the presence of different ethnic groups from the central Chaco and a novel criollo town.
Wirkungen eines deutschen Insolvenz-verfahrens bei Vermögen im Ausland- Unterschiedliche Regelungsansätze im Internationalen Insolvenzrecht am Beispiel Argentiniens
Wirkungen eines deutschen Insolvenz-verfahrens bei Vermögen im Ausland- Unterschiedliche Regelungsansätze im Internationalen Insolvenzrecht am Beispiel Argentiniens
Miguens, Héctor José; Esser, H. Philipp
Régimen del sistema argentino de derecho internacional privado de la insolvencia conforme el art. 4 de la ley concursal argentina vigente con una comparación con el sistema concursal internacional alemán.
Trasplante intestinal en pediatría: Análisis de la primera serie de receptores en la Argentina
Trasplante intestinal en pediatría: Análisis de la primera serie de receptores en la Argentina; Intestinal transplantation in pediatrics: Analysis of the first recipient series in Argentina
Trentadue, Julio; Rumbo, Carolina; Garcia Hervás, Dolores; Saá, Gladys; Martínez, María Inés; Orce, Guillermo; Fernández, Adriana; Gondolesi, Gabriel Eduardo
El presente trabajo comunica la evolución posoperatoria inmediata y alejada de los pacientes pediátricos trasplantados en un único centro en la Argentina, desde marzo de 2006 hasta marzo de 2010, en variables demográficas, indicaciones, contraindicaciones, evolución y supervivencia de pacientes e injerto. Basándose en los resultados se puede concluir que el trasplante intestinal debe ser considerado como un tratamiento válido para todos aquellos pacientes que presenten insuficiencia intestinal, con complicaciones del soporte parenteral. Los resultados adquiridos en nuestro programa son similares a los comunicados internacionalmente y abren una nueva perspectiva para un grupo especial de niños que carecían de solución en nuestro medio.; The present is a retrospective analysis of all pediatric patients that underwent intestinal transplant from march 2006 to march 2010, describing demographics, indications, contraindications, clinical follow up and survival in a single center in Argentina. Based on the results shown one can conclude that intestinal transplant should be considered as a valid treatment for patients with intestinal insufficiency and complications related to parenteral nutrition. The results of our program are similar to those reported in the international Intestinal Transplant Registry. This opens a new perspective to a special population that otherwise would not have any other therapeutic option.
La materialidad de la sombra: Abyección y cuerpo en la definición de la basura
La materialidad de la sombra: Abyección y cuerpo en la definición de la basura
D'hers, Victoria
En las páginas que siguen ahondamos en la relación entre la experiencia de vivir en basurales y la definición propia de la basura desde esa experiencia. ¿Cómo pensar lo definido como tal en ese territorio particular? A partir del análisis de los asentamientos en basurales a cielo abierto, planteamos la necesidad de estudiar qué rol tiene la emoción y la corporalidad en la construcción de esa realidad. Así, profundizamos el análisis bajo la idea de lo abyecto, lo otro teniendo en cuenta que la definición tradicional de basura implica tal estado de ser la sombra, lo desechado. Este análisis toma relevancia en el marco de dominación y violencia simbólica de Latinoamérica en tanto países poscoloniales, bajo lógicas de silenciamiento y subalternización (cfr. Grosso 2007). Sentimos la urgencia política y objetivo central de analizar cómo operan estos silenciamientos, en tanto dinámicas de acostumbramiento a nivel sistemático, en tanto parte de los mecanismos de soportabilidad social (cfr. Scribano). Hacemos referencia tanto a la discusión teórica como a su correlato en el trabajo de campo en asentamientos del conurbano de la Provincia de Buenos Aires, Argentina. Dicho trabajo está aún en proceso, por lo que presentamos aquí líneas de pensamiento en elaboración.
The pyrrhonian argument from possible disagreement
The pyrrhonian argument from possible disagreement
Machuca, Diego Emanuel
In his Pyrrhonian Outlines, Sextus Empiricus employs an argument based upon the possibility of disagreement in order to show that one should not assent to a Dogmatic claim to which at present one cannot oppose a rival claim. The use of this argument seems to be at variance with the Pyrrhonian stance, both because it does not seem to accord with the definition of Skepticism and because the argument appears to entail that the search for truth is doomed to failure. In the present paper, I examine the passages in which Sextus utilizes the argument from possible disagreement and offer an interpretation that makes the use of this argument compatible with the Pyrrhonian outlook.
Los “problemas de crecimiento” de la economía moderna
Los “problemas de crecimiento” de la economía moderna
Crespo, Ricardo Fernando
En este artículo se analizan las consecuencias del reciente renovado interés de la economía en la cuestión de los fines. Se argumenta que el modo adecuado de elegir y tratar con los fines no es el habitual de la economía, diseñado para la asignación eficiente de medios a fines. Se presenta el modo apropiado de hacerlo tras un análisis de los tipos de comparación según Aristóteles.
Hierarchical mesoporous films: from self-assembly to porosity with different length scales
Hierarchical mesoporous films: from self-assembly to porosity with different length scales
Innocenzi, Plinio; Malfatti, Luca; Soler Illia, Galo Juan de Avila Arturo
Hierarchical porous films are formed by interconnected pores of different dimensions, and are particularly attractive for the development of smart materials with multiple functions and enhanced transport properties. We have focused this review on hierarchical porous films whose synthesis, at least at one scale of porosity, is based on self-assembly. Several strategies have been proposed for the preparation of hierarchical porous films which for bulks and monoliths are more difficult to obtain. These materials represent a step further in the fabrication of complex materials through self-assembly, and achieving order through templating routes at different length scales is the ultimate goal.
Canopy structure analysis for estimating forest regeneration dynamics and growth in Nothofagus pumilio forests.
Canopy structure analysis for estimating forest regeneration dynamics and growth in Nothofagus pumilio forests.
Martínez Pastur, Guillermo José; Peri, Pablo Luis; Cellini, Juan Manuel; Lencinas, María Vanessa; Barrera, Marcelo Daniel; Ivancich, Horacio Simón
Introduction Silviculture systems applied in Nothofaguspumilio forests are based on opening the canopy tostimulate natural regeneration by modifying light and soilmoisture. The objective is to evaluate regeneration dynamicsof N. pumilio along different forest canopy and solarradiation gradients.& Materials and methods Regeneration data and seedproduction were obtained in 98 permanent plots establishedin old-growth and harvested stands along a crown covergradient (19?93%). Volumetric soil water content and basalarea were also measured, while crown cover and solarradiation transmission were estimated using hemisphericalphotographs. Sapling height was modeled using plant age,crown cover, and solar radiation transmission of the standsas explanatory variables.& Results Natural regeneration dynamics were closelyrelated to crown cover values, which determined seedproduction and the successful establishment and subsequentregeneration growth. Height growth was found to be relatedto crown cover and solar radiation transmission, where N.pumilio regeneration is optimized at intermediate crowncover levels (45% crown cover, 0.5 effective leaf areaindex, 26 W m2 global radiation, and 65% percentage ofglobal radiation).& Conclusion These findings can be used to develop newsilvicultural methods or to adjust the current practices toensure regeneration establishment and maximize heightgrowth at the stand level.
Phospholipases A in Trypanosomatids
Phospholipases A in Trypanosomatids
Belaunzarán, María Laura; Lammel, Estela María; de Isola, Elvira Luisa Durante
Phospholipases are a complex and important group of enzymes widespread in nature, that play crucial roles in diverse biochemical processes and are classified as A1, A2, C, and D. Phospholipases A1 and A2 activities have been linked to pathogenesis in various microorganisms, and particularly in pathogenic protozoa they have been implicated in cell invasion. Kinetoplastids are a group of flagellated protozoa, including extra- and intracellular parasites that cause severe disease in humans and animals. In the present paper, we will mainly focus on the three most important kinetoplastid human pathogens, Trypanosoma brucei, Trypanosoma cruzi, and Leishmania spp., giving a perspective of the research done up to now regarding biochemical, biological, and molecular characteristics of Phospholipases A1 and A2 and their contribution to pathogenesis.
Lower complexity bounds for interpolation algorithms
Lower complexity bounds for interpolation algorithms
Gimenez, Nardo Ariel; Heintz, Joos Ulrich; Matera, Guillermo; Solernó, Pablo Luis
We introduce and discuss a new computational model for the HermiteLagrange interpolation with nonlinear classes of polynomial interpolants. We distinguish between an interpolation problem and an algorithm that solves it. Our model includes also coalescence phenomena and captures a large variety of known HermiteLagrange interpolation problems and algorithms. Like in traditional HermiteLagrange interpolation, our model is based on the execution of arithmetic operations (including divisions) in the field where the data (nodes and values) are interpreted and arithmetic operations are counted at unit cost. This leads us to a new view of rational functions and maps defined on arbitrary constructible subsets of complex affine spaces. For this purpose we have to develop new tools in algebraic geometry which themselves are mainly based on Zariski's Main Theorem and the theory of places (or equivalently: valuations). We finish this paper by exhibiting two examples of Lagrange interpolation problems with nonlinear classes of interpolants, which do not admit efficient interpolation algorithms (one of these interpolation problems requires even an exponential quantity of arithmetic operations in terms of the number of the given nodes in order to represent some of the interpolants). In other words, classic Lagrange interpolation algorithms are asymptotically optimal for the solution of these selected interpolation problems and nothing is gained by allowing interpolation algorithms and classes of interpolants to be nonlinear. We show also that classic Lagrange interpolation algorithms are almost optimal for generic nodes and values. This generic data cannot be substantially compressed by using nonlinear techniques. We finish this paper highlighting the close connection of our complexity results in HermiteLagrange interpolation with a modern trend in software engineering: architecture tradeoff analysis methods (ATAM).
A comparative study of the O-3 reactivity of isomeric N-dimethylmaleoyl- protected d-glucosamine and d-allosamine acceptors
A comparative study of the O-3 reactivity of isomeric N-dimethylmaleoyl- protected d-glucosamine and d-allosamine acceptors
Colombo, María Inés; Stortz, Carlos Arturo; Ruveda, Edmundo
Four isomeric N-dimethylmaleoyl 4,6-O-benzylidene-protected d-hexosamine acceptors (2, 3, 4, and 5) with all possible configurations at C-1 and C-3 (e.g., derived from d-glucosamine and d-allosamine) were prepared, and the assessment of their O-3 relative reactivity through competition experiments using the known per-O-acetylated d-galactopyranosyl trichloroacetimidate donor (15) was then carried out. The reactivities are in the order 4 ≫ 2 > 5 > 3. The analysis of the NMR spectra of 2-5 at different temperature and modeling experiments carried out on analogs of 2-5 (DFT) and on the acceptors themselves (MM) are coincident, and have helped to establish the stability of the different hydrogen bonds, and of the conformers which carry them. The whole results suggest that the electronic effects (hydrogen bonds) are required to explain the observed trend, in spite of the axial conformation of the most reactive hydroxyl group. The steric effects appear only when hydrogen bonds are weak.
Riesgo y derecho, perspectivas sobre el uso de agroquímicos en Argentina
Riesgo y derecho, perspectivas sobre el uso de agroquímicos en Argentina
Berros, María Valeria
El presente trabajo intenta efectuar un aporte sobre la relación entre derecho y riesgos. Para ello se utilizan elementos provenientes de un análisis de caso, el uso de agroquímicos en Argentina, como disparador para escrutar en la temática propuesta. Se recorren las líneas generales de la institucionalización del tema en el ámbito de las agencias estatales y las legislaciones nacionales y locales, lo que permite visibilizar la injerencia de las ciencias y las técnicas en la arquitectura jurídica. Por otro lado, se indaga cómo se traducen las diversas percepciones sociales respecto de la introducción de este riesgo, focalizando en la emergencia de algunos conflictos socio-ambientales y su re-significación al franquear las fronteras de ingreso al campo jurídico.
Relevance of biofilms in pediatric tonsillar disease
Relevance of biofilms in pediatric tonsillar disease
Diaz, R. R.; Picciafuoco, S.; Paraje, María Gabriela; Villegas, N. A.; Miranda, J. A.; Albesa, Inés; Cremonezzi, David César; Commisso, R.; Paglini Oliva, P.
In this investigation, we study the relation between chronic inflammation of the tonsils, clinical features, and the presence of biofilms in the crypts in patients presenting with obstructive hypertrophy and recurrent upper airway pathology. Thirty-six patients who needed to undergo a tonsillectomy for obstructive reasons (aged 1 to 6 years), among which none of them had taken any antibiotics 30 days prior to surgery, were included. Samples were examined with hematoxylin-eosin and Gram staining, fluorescent microscopy, and confocal laser microscopy. The predominance of symptoms were those related to obstructive pathology rather than infection (p∈<∈0.01). All patients had tonsillar hypertrophy (grade III or IV), but an association with adenoids hypertrophy was detected in 66.66% of cases (p∈<∈0.05). 77.28% of tonsils presented biofilms in their crypts, but hypertrophy and tonsillar follicle number were not related to the presence or absence of biofilms. Here, we demonstrated that symptoms like harsh raucous sound, tonsillar and adenoids hypertrophy, apnea, and cervical adenopathies are clearly related to the presence of biofilm in tonsils. Our results allow us to propose that biofilms are involved in the pathogenesis of tonsils and adenoids hypertrophy. The prevention of biofilms formation should be focused in the early stages, attempting to restrain bacterial attachment to the respiratory mucosa.
Convergence of an adaptive Kačanov FEM for quasi-linear problems
Convergence of an adaptive Kačanov FEM for quasi-linear problems
Garau, Eduardo Mario; Morin, Pedro; Zuppa, Carlos
We design an adaptive finite element method to approximate the solutions of quasi-linear elliptic problems. The algorithm is based on a Kačanov iteration and a mesh adaptation step is performed after each linear solve. The method is thus inexact because we do not solve the discrete nonlinear problems exactly, but rather perform one iteration of a fixed point method (Kačanov), using the approximation of the previous mesh as an initial guess. The convergence of the method is proved for any reasonable marking strategy and starting from any initial mesh. We conclude with some numerical experiments that illustrate the theory.
El trabajo infantil como estrategia de sostén de las familias pobres en la Argentina, la necesidad de rediseñar el objeto de las políticas públicas
El trabajo infantil como estrategia de sostén de las familias pobres en la Argentina, la necesidad de rediseñar el objeto de las políticas públicas; Child labour as support for poor families in Argentina:the need to revise the objectives of public policies
Noceti, Maria Belen
La siguiente ponencia pretende discutir a través del análisis de datos obtenidos durante el desarrollo de Proyectos de Investigación Acción llevados adelante desde la Universidad Nacional del Sur, Argentina, la necesidad de rediseñar las políticas publicas existentes en torno a la erradicación del trabajo infantil en la Argentina. En la actualidad este fenómeno social se aborda teniendo como eje de trabajo los individuos, esta ponencia propone que el eje de trabajo necesariamente deben ser los grupos de referencia de los niños trabajadores ya sean grupos de pares o familias, puesto que el trabajo infantil se gesta en una red de relaciones sociales. Siendo así si se erradica debe ofrecer otra red de contención al niño ya que la necesidad de afiliación social es uno de los derechos que como ser humano debe prevalecer. Las políticas publicas deben generar dispositivos de afiliación social que reinserten a los niños en el sistema de educación formal, para ello la educación popular es un instrumento y quizás los técnicos deban diseñar muchos más en función de las características culturales de cada población, en este sentido la antropología puede ofrecer interesantes aportes.; The aim of this research is to discuss the need to revisethe current public policies on the eradication of child labour in Argen-tina by means of the analysis of the data obtained during the develop-ment of research projects carried out by the Universidad Nacional delSur in Argentina. Currently, this social phenomenon is analysed fromthe point of view of the individual. This research suggests that the focusshould instead shift to the working children’s reference groups, whethercouples or families, since child labour is the outcome of a network ofsocial relations. Hence, if child labour is eradicated a different meansof contention should be offered to the child since the need for social affiliation is one of the rights which should prevail for the child as ahuman being. Public policies should create tools for social affiliation facilitating the child’s incorporation into the formal education system.Popular education is a useful tool for achieving this objective, which iswhy professionals should design tools by taking into consideration thecultural aspects of different communities. In this sense, anthropology might offer interesting input.
Earthquake deformation in the northwestern Sierras Pampeanas of Argentina based on seismic waveform modelling
Earthquake deformation in the northwestern Sierras Pampeanas of Argentina based on seismic waveform modelling
Alvarado, Patricia Monica; Ramos, Victor Alberto
We investigate the seismic properties of modern crustal seismicity in the northwestern Sierras Pampeanas of the Andean retroarc region of Argentina. We modelled the complete regional seismic broadband waveforms of two crustal earthquakes that occurred in the Sierra de Velasco on 28 May 2002 and in the Sierra de Ambato on 7 September 2004. For each earthquake we obtained the seismic moment tensor inversion (SMTI) and tested for its focal depth. Our results indicate mainly thrust focal mechanism solutions of magnitudes Mw 5.8 and 6.2 and focal depths of 10 and 8km, respectively. These results represent the larger seismicity and shallower focal depths in the last 100 years in this region. The SMTI 2002 and 2004 solutions are consistent with previous determinations for crustal seismicity in this region that also used seismic waveform modelling. Taken together, the results for crustal seismicity of magnitudes ≥5.0 in the last 30 years are consistent with an average P-axis horizontally oriented by an azimuth of 125° and T-axis orientation of azimuth 241° and plunge 58°. This modern crustal seismicity and the historical earthquakes are associated with two active reverse faulting systems of opposite vergences bounding the eastern margin of the Sierra de Velasco in the south and the southwestern margin of the Sierra de Ambato in the north. Strain recorded by focal mechanisms of the larger seismicity is very consistent over this region and is in good agreement with neotectonic activity during the last 11,000 years by Costa (2008) and Casa et al. (in press); this shows that the dominant deformation in this part of the Sierras Pampeanas is mainly controlled by contraction. Seismic deformation related to propagation of thrusts and long-lived shear zones of this area permit to disregard previous proposals, which suggested an extensional or sinistral regime for the geomorphic evolution since Pleistocene.
Candida albicans Tpk1p and Tpk2p isoforms differentially regulate pseudohyphal development, biofilm structure, cell aggregation and adhesins expression
Candida albicans Tpk1p and Tpk2p isoforms differentially regulate pseudohyphal development, biofilm structure, cell aggregation and adhesins expression
Giacometti, Romina; Kronberg, Maria Florencia; Biondi, Ricardo Miguel; Passeron, Susana
Candida albicans undergoes a reversible morphological transition from single yeast cells to pseudohyphal and hyphal filaments. In this organism, cAMP-dependent protein kinase (PKA), coded by two catalytic subunits (TPK1 and TPK2) and one regulatory subunit (BCY1), mediates basic cellular processes, such as the yeast-to-hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, although distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different BCY1 genetic backgrounds. We observed that under hypha-only inducing conditions, all BCY1 heterozygous strains shifted growth toward pseudohyphal morphology; however, the pseudohypha:hypha ratio was higher in strains devoid of TPK2. Under pseudohypha-only inducing conditions, strains lacking TPK2 were prone to develop short and branched pseudohyphae. In tpk2Δ/tpk2Δ strains, biofilm architecture was markedly less dense, composed of short pseudohyphae and blastospores with reduced adhesion ability to abiotic material, suggesting a significant defect in cell adherence. Immunolabelling assays showed a decreased expression of adhesins Als1p and Als3p only in the tpk2Δ/tpk2Δ strain. Complementation of this mutant with a wild-type copy of TPK2 restored all the altered functions: pseudohyphae elongation, biofilm composition, cell aggregation and adhesins expression. Our study suggests that the Tpk2p isoform may be part of a mechanism underlying not only polarized pseudohyphal morphogenesis but also cell adherence.
Role of MMPs in Metastatic Dissemination: Implications for Therapeutic Advances
Role of MMPs in Metastatic Dissemination: Implications for Therapeutic Advances
Simian, Marina; Raffo, Diego Alejandro; Pontiggia, Osvaldo Alejandro
Matrix metalloproteinases (MMPs) have been implicated in both normal and pathologic processes. In cancer in particular, in vitro and animal studies showed an involvement of MMPs in many stages of cancer progression. This led to the development of MMP inhibitors that in most cases failed in clinical trials. In this review we go over the role of MMPs in the different stages of cancer progression and try to understand why the early generation of MMP inhibitors failed. The analysis of the lessons from this first experience, plus the review of the current knowledge that shows that MMPs may be pro-or anti-tumorigenic may set the stage for a future success for this therapeutic strategy in cancer.
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