Determination of 2-hydroxy-4-methoxybenzophenone in mice serum and human plasma by ultra-high-performance liquid chromatography enhanced by chemometrics Teglia, Carla Mariela; Santamaría, Clarisa Guillermina; Rodriguez, Horacio Adolfo; Culzoni, Maria Julia; Goicoechea, Hector Casimiro This work presents the development of a strategy for the quantitation of 2-hydroxy-4-methoxybenzophenone (BP-3) in mice serum and human plasma. The instrumental determination only lasts 2 min and is based on ultra-high-performance liquid chromatography (UHPLC) coupled to second-order data modeling with multivariate curve resolution-alternating least-squares (MCR-ALS). Coelution of BP-3 with the matrix components was resolved by exploiting the second-order advantage of multi-way calibration. The validation study led to the following results: linear range from 0.002 to 1.509 μg mL−1; in the case of mice serum samples: recoveries from 95 to 98%, with intra and inter day precision of 2.7 and 5.7, respectively; and in the case of human plasma samples: recoveries from 94 to 99% with intra and inter day precision of 2.3 and 4.6%, respectively. Special emphasis was put on the determination of the LOD and LOQ by utilizing several approaches for their calculation and making focus on the experimental verification of the figures provided by the multivariate methodology. Following the mentioned approach, the LOD and LOQ of the method were estimated as 0.66 and 2.00 ng mL−1, respectively. Eventually, the method was successfully applied to the determination of BP-3 in mice serum.

Enzymatic synthesis of non-natural trisaccharides and galactosides; Insights of their interaction with galectins as a function of their structure Porciúncula González, Cecilia; Cagnoni, Alejandro; Mariño, Karina Valeria; Fontana, Carolina; Saenz Méndez, Patricia; Irazoqui, Gabriela; Giacomini, Cecilia Galectins are a family of carbohydrate-recognizing proteins that by interacting with specific glycoepitopes can mediate important biological processes, including immune cell homeostasis and activation of tolerogenic circuits. Among the different members of this family, Galectin 1 and 3 have shown pro-tumorigenic effects, being overexpressed in numerous neoplasic diseases, proving to be relevant in tumor immune escape, tumor progression and resistance to drug-induced apoptosis. Thus, generation of specific glycosides that could inhibit their pro-tumorigenic ability by blocking their carbohydrate recognition domain is one of the current major challenges in the field. Considering that galectin-ligand binding strength is closely related to the ligand structure, analysis of this relationship provides valuable information for rational design of high-affinity ligands that could work as effective galectin inhibitors. Taking profit of the ability of glycosidases to catalyze transglycosylation reactions we achieved the enzymatic synthesis of β- D -Galp-(1 → 6)-β- D -Galp-(1 → 4)- D -Glcp (2), a mixture of β- D -Galp-(1 → 6)-β- D -Glcp-(1 → 4)- D -Glcp (5) and β- D -Galp-(1 → 3)-β- D -Glcp-(1 → 4)- D -Glcp (6), and finally benzyl β-D -galactopyranoside (9), with reaction yields between 16 and 27%. All the galactosides were purified, and characterized using 1 H and 13 C nuclear magnetic resonance spectroscopy. Docking results performed between the synthesized compounds and human Galectin 1 (hGal-1) and human Galectin 3 (hGal-3) showed that the replacement of a glucose moiety linked to the terminal galactose with a galactose moiety, decreases the affinity for these galectins. Moreover, regarding the interglycosidic bond the most favorable β-Gal linkage seems to be β(1 → 4) followed by β(1 → 3) and β(1 → 6) for hGal-1, and β(1 → 4) followed by β(1 → 6) and β(1 → 3) for hGal-3. These results were in accordance with the IC50 values obtained with in vitro solid phase inhibition assays. Therefore, docking results obtained in this work proved to be a very good approximation for predicting binding affinity of novel galactosides.

The Puesto Piris Formation: Evidence of basin-development in the North Patagonian Massif during crustal extension associated with Gondwana breakup Strazzere, Leonardo; Gregori, Daniel Alfredo; Benedini, Leonardo; Marcos, Paulo; Barros, Mercedes Virginia; Geraldes, Mauro C.; Pavon Pivetta, Cecilia Mariana The Marifil Volcanic Complex, exposed in the eastern North Patagonian Massif, Argentina, includes up to 550 m of red conglomerates, sandstones, black siltstones, limestones, and reworked tuff of the Puesto Piris Formation. The basal part of this unit, which was deposited in high-gradient topographic relief, is composed of conglomerates and sandstones with thin layers of reworked tuffs. The lithofacies associations of the basal part indicate that the depositional mechanisms were mantled and gravitational flows. The middle part of the unit consists of fine sandstones, limestones, and black siltstones that were deposited in low-energy fluvial and lacustrine environments. The outcrops are located along the NE–SW direction and the major thickest units represented by limestones and siltstones, occur near the southeastern border of this NE–SW depocenter. Since the rhyolitic and trachytic lava flows and tuffs of the Marifil Volcanic Complex are interbedded with the sedimentary sequences of the Puesto Piris Formation, both units are coeval. Zircon U–Pb age was obtained for a trachytic lava flow (193.4 ± 3.1 Ma) suggesting that sedimentation and volcanism are Sinemurian. This extensional episode was recorded in the eastern, western, and southwestern sectors of the North Patagonian Massif, and is possibly associated with the Gondwana supercontinent breakup.

Nitrogen balance in a plant-soil system under different cover crop-soybean cropping in Argentina Landriscini, María Rosa; Galantini, Juan Alberto; Duval, Matias Ezequiel; Capurro, Julia E. Cover crops (CC) provide many benefits for the soil and the following crop but their effects on nitrogen (N) release and balance in continuous no-tillage soybean (Glycine max L. Merr.) production are little known. Estimation of the biological nitrogen fixation (BNF) in intensive agricultural systems under soybean is essential to understanding the N dynamics and to determining the balances and crop demands. This study (2006–2011) was performed on a Typic Argiudoll under no-tillage in the province of Santa Fe, Argentina. The aims were to study the effect of fall winter CC, such as wheat (W), oats (O), oats + vetch (O + V) and vetch (V), on the yield and N-content of the following crop (soybean) and to quantify the contribution of the BNF and N-balance. Three methodologies were used for BNF estimation: 1) a linear regression model between BNF and N-uptake by soybean; 2) the natural 15N abundance in soybean and 3) the average BNF in the Pampa region. Gramineous CC developed more dry matter than pure legume species, with intermediate values for the gramineous-legume mixture. Biological fixation provides 60–70% of absorbed N, according to the estimation method. Within the rainfall range of 500–1000 mm during the soybean cycle, CC did not affect the grain yield or soybean dry matter production. The partial N-balance was always positive, with differences between the techniques used for BNF estimation. Cover crops have contributed to the positive soil N-balance. Gramineous CC stored 22% more N-content in the soil surface layer than the others. Cover crops showed 15% higher index of N-stratification on the surface compared to the control soil. Using CC would be an efficient alternative to produce biomass and to supply N to the soil for the subsequent crop.

New remains of Nothrotheriinae (Mammalia, Xenarthra) from Late Pleistocene fluvial deposits of Northern Pampa (Santa Fe Province, Argentina) Vezzosi, Raúl Ignacio; Brandoni, Diego; Brunetto, Ernesto; Zalazar, Maria Cecilia In this contribution the record of a Nothrotheriinae (Xenarthra, Tardigrada) ground sloth is reported from the Late Pleistocene of the Northern Pampa of Santa Fe Province, Argentina. The stratigraphic unit where the fossil was collected corresponds to fluvial-palustrine sediments of the Timbúes Formation, outcropping along the Carcarañá River valley. The relative stratigraphic position of this lithostratigraphic unit, observed in several sections mainly on the Paraná River cliffs near Carcarañá River distal area, suggests that it was deposited during the Last Interglacial Stage. The specimen (MPAHND-135), assigned to Nothrotherium cf. torresi, is represented by the proximal two-thirds of a left femur that shows a prominent lesser trochanter and no connection between the third trochanter and the ectepicondyle. The presence of Nothrotherium cf. torresi in sediments related to humid conditions supports the idea that Nothrotheriinae had great ecological tolerance and was capable of inhabiting climates ranging from cold and arid, as was previously proposed, to warm and humid.

Thermal evolution of upper crustal magmatic systems from the Sierra de Velasco, NW Argentina Macchioli Grande, Marcos Simón; Alasino, Pablo Horacio; Rocher, Sebastian; Larrovere, Mariano Alexis; Uran, Gimena Mariel; Reinoso Carbonell, Virginia Valeria; Moreno, Gabriel Detailed mapping of the Huaco intrusive Complex in NW Argentina documents a shift in the emplacement style of successive magma additions in an upper crustal environment as the system evolves thermally. The units of the Huaco intrusive complex exhibit a change from a tabular to funnel-shaped subhorizontal body (Huaco unit) with stoping and brittle fracturing as main emplacement mechanisms, to a cylindrical body (Sanagasta unit), emplaced in a heated host rock which shows local ductile behaviour. As the system cools, the youngest intrusion (La Chinchilla unit) emplaces again as a subhorizontal tabular to funnel-shaped body. Furthermore, magmatic fabrics recorded a regional strain field that changed from a SW-NE to WSW-ENE shortening, as well as boundary strain increments and stress reorientation. Our results demonstrate that the evolution of magma chambers involves the emplacement of magma batches in a stiff crust, changing its thermal conditions and maturing the system for following intrusions. The latter ascend as visco-elastic elongated bodies using the same magmatic plumbing system, leading to a shift in the emplacement style. The described evolution is similar to that reported for the contemporaneous San Blas intrusive Complex located in the same range. This allows us to affirm that these processes are recurrent in the upper crustal section, and that the thermal and rheological conditions of the crust are locally modified by the formation and evolution of long-lived magma reservoirs.

El diablo y su cola en el Lago Titicaca Costilla, Julia El santuario boliviano de Copacabana como ejemplo del mestizaje religioso de catolicismohispánico con creencias indígenas legado por la sociedad colonial.

Phylogenetic placement of the Peruvian-endemic genus Machaerophorus (Brassicaceae) based on molecular data and implication for its systematics Salariato, Diego Leonel; Cueva Manchego, Marco A.; Cano, Asunción; Al-Shehbaz, Ihsan The genus Machaerophorus includes three perennial species (M. arequipa, M. laticarpus, and M. matthioloides) inhabiting the southern Peruvian Andes. However, based on the morphological data, this genus has been reduced for more than one hundred years to synonymy of several genera of various tribes, and its phylogenetic placement using molecular data has never been studied. Therefore, in this study we explored the phylogenetic placement of Machaerophorus to test its generic status and tribal assignment, using for this purpose ribosomal nuclear (ITS) and chloroplast (trnL-F, trnH-psbA, rps16 intron) data together with a comprehensive sampling of taxa. Results obtained here demonstrated that Machaerophorus represents a well-differentiated lineage within the South American Cremolobeae–Eudemeae–Schizopetaleae clade, and hence, the genus is reinstated and excluded from the placements in Sibara and Mathewsia. Based on molecular data and morphological affinities, we included it within the tribe Schizopetaleae. A key to Machaerophorus and the genera of Schizopetaleae is also presented.

Spatial organization and habitat selection of Geoffroy's cat in the Espinal of central Argentina Castillo, Diego Fabián; Luengos Vidal, Estela Maris; Caruso, Nicolás; Manfredi, Matilde Claudia; Lucherini, Mauro; Casanave, Emma Beatriz We report data on the spatial ecology and habitat selection of eight adult Geoffroy's cat Leopardus geoffroyi (five males and three females) that were radiotracked in an area of the central Argentine Espinal. Mean home range size varied from 2.2 ± 1.9 km2 (Kernel95%) to 2.8 ± 2.4 km2 (MCP100%), with male home ranges 4.1 larger than those of females. Core areas (Kernel50%) averaged 0.7 ± 0.7 km2 and were 3 times smaller for females. Daily movement patterns were consistent with the variations in home range sizes being greater in males (590.2 m ± 476.6) than females (413.5 m ± 288.1). Home range overlap averaged 38%, and was highly variable between males and females and reached its highest value for intersexual pairs. At second-order resolution, most of individuals had a strong selection for open woodland, while the other habitats were generally avoided. At the home range level, although the electivity index values for the open woodland were positive for all animals, habitat selection showed a high inter-individual variation. Our data support previous studies that suggest that Geoffroy's cats show a certain degree of flexibility in their spatial behavior. Finally, we argue that natural woodland patches or habitats with dense vegetation are important for L. geoffroyi in the Argentinean Espinal and their alteration can affect the conservation status of this cat.

Electronic transport in a graphene single layer: Application in amino acid sensing Rodríguez Sotelo, Sindy Julieth; Albanesi, Eduardo Aldo We modeled a type of field-effect transistor device based on graphene for the recognition of amino acids with a potential application in the building of a protein sequencer. The theoretical model used was a combination of density functional theory (DFT) with the non-equilibrium Green's function (NEGF) in order to describe the coherent transport in molecular devices. First, we studied the physisorption of each amino acid on a graphene sheet and we reported the adsorption energy, the adsorption distances, the equilibrium configuration and the charge transfer of ten amino acids that can be considered as representative of all of the amino acids: histidine (His), alanine (Ala), aspartic acid (Asp), tyrosine (Tyr), arginine (Arg), glutamic acid (Glu), glycine (Gly), phenylalanine (Phe), proline (Pro) and lysine (Lys). As a result, significant differences were found in the density of states (DOS) after adsorption and there was a change in the semi-metallic character of the graphene due to the lysine and arginine interactions. Furthermore, we noticed changes in the electrical characteristics of the devices, as the amino acids adsorbed onto the surface of the graphene. The curves of current vs. bias voltage (I-Vb) display a distinct response for each amino acid, i.e. the I-Vb curves produce a characteristic footprint for each amino acid. We identified a possible rectification mechanism related to the voltage profile asymmetry, where the amino acids can control the transport characteristics in the device, i.e. Lys and Phe amino acids physisorbed on graphene act as a molecular diode, where electrons can easily flow in one direction and decrease in the other. This may be promising for the prospect of biosensors: graphene could be used as an amino acid detector.

Temporomandibular joint osteoarthritis in human ancient skeletal remains from Late Holocene in southern Patagonia Suby, Jorge Alejandro; Giberto, Diego Agustin Low prevalence of caries, high stages of dental attrition (DA), and moderate frequency of antemortem tooth loss (AMTL) were found in skeletal remains of hunter-gatherers from southern Patagonia. However, osteoarthritis (OA) of temporomandibular joint (TMJ), possible related to oral health, has not been studied so far. The influence of hunter-gatherer living conditions on the development of TMJ-OA was scarcely evaluated, and analyses of degenerative changes of TMJ in nonindustrialized groups are needed. The aim of this study is to analyse the association between degenerative changes of TMJ, oral disease, and palaeodietary patterns in skeletal remains of hunter-gatherers from southern Patagonia. Degenerative changes of TMJ were recorded in 25 adult skulls of both sexes. Eburnation of articular eminence and/or mandibular condyle was considered as pathognomonic of OA. In the absence of eburnation, OA was diagnosed when two or more of the following lesions were identified: (a) new bone formation on joint surface, (b) new bone formation of joint margins, (c) porosity on the joint surface, and (d) modification of joint contour. In the studied skeletons, it was found that OA prevalence is 28%, similar to those observed in other hunter-gatherer populations elsewhere. TMJ-OA is mostly related to AMTL and older age, but no relation was found with caries and DA, sex, region, dietary patterns, and chronology. However, consumption of terrestrial resources in the diet cannot be discarded as a related factor. Further studies are necessary to explore the morphological variability of the mandible and its possible relationship with different diets and dental pathologies in these populations.

Las relaciones con el Bloque del Este desde el punto de vista de las emociones de los diplomáticos argentinos en tiempos de Guerra Fría. El caso de las campañas de repatriación a los países socialistas (1955-1961) Galván, Maria Valeria Este trabajo analiza los discursos de los diplomáticos argentinos en legaciones del Bloque Comunista en Europa del Este, acerca de las campañas de repatriación socialistas que afectaron a las comunidades locales de inmigrantes eslavos en Argentina, entre los años 1955-1961.Las valoraciones de los diplomáticos sobre las consecuencias de la campaña para la población local y para la gestión estatal argentina sirvieron como base para las presiones que la Cancillería ejerció regularmente sobre los organismos de inteligencia dependientes del Ministerio del Interior, con el objetivo de reforzar la seguridad interna y controlar los efectos de esta campaña propagandística en suelo argentino.; This paper analyzes the discourse of Argentine diplomats in countries of the Communist Bloc in Eastern Europe on the Socialist repatriation campaigns and their consequences on the local communities of Slavic immigrants in Argentina, between the years 1955-1961. The emotional attachment of the diplomats towards the consequences of the campaign on the local population and on the local State administration served as a basis for the claims that the Argentinean Foreign Office presented regularly to the intelligence divisions of the Ministry of Internal Affairs, in order to reinforce internal security and mechanisms of control against the impact of this propaganda campaign on Argentinean soil.

Hacia una analítica del sensorium común: Apuntes para una morfología estético-política de lo social; Towards an analysis of the common sensorium: Notes for an aesthetic-political morphology of the social dimension Mallamaci, Marco German Tanto frente al avance de los medios electrónicos y las teletransmisiones del siglo XX como frente a la digitalización cibernética del siglo XXI, los diversos campos epistémicos se han encargado de problematizar la relación entre lo perceptivo, lo estético (espacio-tiempo) y los cambios tecnológicos que imponen nuevas prácticas intersubjetivas, comportamientos y mod os de subjetivación. En muchos autores surge el uso de un concepto puntual: el sensorium. Ya sea que se proponga un enfoque cultural o una perspectiva específicamente gnoseológica, suele r epetirse una referencia de dicha categoría en relación con los cambios de la velocidad y la aceleración de las imágenes, los textos y las comunicaciones; primero en la era de la electrónica analógica y luego en la época digital. El objetivo de este trabajo es exponer una breve sistematización del concepto de sensorium en vistas de una posible definición en tanto unidad de análisis estético-política. Dicha categoría permite, por un lado, pensar la disciplina Estética como pensamiento político ; y por el otro, pensar lo político en clave estética. Para ello el texto repasa los diversos sentidos que ha tenido la categoría a lo largo de la historia, según su emergencia en diferentes epistemes. La primera parte recorre ciertos discursos puntuales identificando autores en los cuales se pueden encontrar usos específicos (Newton, Simmel, Benjamin, McLuhan, Kittler, etc.), delineando así una serie de sentidos relacionados con el espacio y el tiempo desde lo estético, lo perceptivo, lo gnoseológico, lo sociológico y lo técnico. A partir de dicho esquema, en la segunda parte se presenta la idea de un ?giro sensorial? en los estudios sociopolíticos contemporáneos, donde la idea de sensorium cobra una nueva importancia. Por último, se hace foco en las relaciones conceptuales que se derivan del uso que propone Rancière en torno a la categoría. En base a dicho recorrido el texto busca delinear una serie de elementos para abrir la posibilidad de definir la Estética como el pensamiento del sensorium común; se trata de una unidad de análisis estético-política que funciona como marco teórico para una morfología de lo social en tanto analítica del orden de lo sensible.; Both, be it the advance of electronic media and teletransmissions in the 20th century and the cyber digitalization of the 21st century, the various epistemic fields have been responsible for problematizing the relationship between the perceptive, the aesthetic (space-time) and the technological changes that impose new intersubjective practices, behaviors and modes of subjectivation. A specific concept arises in many authors: the sensorium. Whether a cultural approach or a specifically epistemological perspective is proposed, a reference to that category is often repeated in relation to changes in the speed and acceleration of images, texts and communications; first in the era of analog electronics and then in the digital age. The objective of this paper is to expose a brief systematization of the sensorium towards a possible definition as an aesthetic-political unit of analysis. This category let us think, on the one hand, about the Aesthetic discipline as political thought, and on the other, about politics in aesthetic terms. For this, this paper reviews various senses that the category of sensorium has had throughout history, according to its emergence in different epistemes. The first part identifies certain discourses locating authors in which specific uses can be found (Newton, Simmel, Benjamin, McLuhan, Kittler, etc.), thus it is possible to delineate a series of senses related to time and space from an aesthetic point of view, from the perception, social realm and the technique. Henceforth, the second part presents the idea of a “sensory turn” in contemporary social and political studies, where the idea of sensorium takes a new importance. Finally, the text focuses on the conceptual relationships that derive from Rancière's use of the category. Based on this lines, this paper seeks to delineate a series of elements to open the possibility of defining the Aesthetic as the thought of the common sensorium; as a unit of aesthetic-political analysis that works as a theoretical framework for a morphology of the social dimension, as analytical of the sensible order.

“El más extraño pensamiento”. Sobre algunas lecturas marxistas del Quijote; “The strangest thought”. On some Marxist readings of Quixote Vedda, Miguel Angel El artículo se ocupa de analizar algunas interpretaciones marxistas del Quijote de Cervantes. Se concentra, ante todo, en las lecturas realizadas por Ernst Bloch (en Espíritu de la Utopía y El principio esperanza) y György Lukács (Teoría de la novela y el ensayo “Don Quijote”), y pone las diferentes interpretaciones en relación con el pensamiento filosófico de ambos autores en sus respectivos períodos.; The article analyzes some Marxist interpretations of Cervantes’ Don Quixote. It focuses on the readings made by Ernst Bloch (in Spirit of Utopia and The Principle of Hope) and György Lukács (The Theory of the novel and the essay Don Quixote), and puts the different interpretations in relation to the philosophical thinking of both authors in their respective periods.

Beyond Stability Constraints: A biophysical model of enzyme evolution with selection on stability and activity Echave, Julián The rate of evolution varies among sites within proteins. In enzymes, two rate gradients are observed: rate decreases with increasing local packing and it increases with increasing distance from catalytic residues. The rate-packing gradient would be mainly due to stability constraints and is well reproduced by biophysical models with selection for protein stability. However, stability constraints are unlikely to account for the rate-distance gradient. Here, to explore the mechanistic underpinnings of the rate gradients observed in enzymes, I propose a stability-activity model of enzyme evolution, MSA. This model is based on a two-dimensional fitness function that depends on stability, quantified by Î "G, the enzyme's folding free energy, and activity, quantified by Î "G∗, the activation energy barrier of the enzymatic reaction. I test MSA on a diverse data set of enzymes, comparing it with two simpler models: MS, which depends only on Î "G, and MA, which depends only on Î "G∗. I found that MSA clearly outperforms both MS and MA and it accounts for both the rate-packing and rate-distance gradients. Thus, MSA captures the distribution of stability and activity constraints within enzymes, explaining the resulting patterns of rate variation among sites.

Rol de los ARN no codificantes en la respuesta al estrés oxidativo en condiciones fisiológicas y en el cáncer Galigniana, Natalia Maricel ARNs no codificantes largos transcriptos a partir de los telómeros,conocidos como TERRA (telomeric repeat-containing RNA), se asocian a laestabilidad telomérica y genómica. Están aumentados en tejidos tumoraleshumanos y murinos, aunque poco se sabe sobre los mecanismos que subyacendicho aumento y las funciones que cumplen. El estrés oxidativo daña biomoléculasy activa vías de señalización asociadas a la proliferación celular, angiogénesis,apoptosis y metástasis. Los telómeros son regiones del genoma especialmentesusceptibles a sufrir daño oxidativo y se ha reportado que la inflamación crónica invivo induce disfunción telomérica por sobreproducción de ROS vía COX-2, lo cuales revertido por tratamiento antiinflamatorio ó antioxidante. Consecuentemente,los objetivos de este trabajo de Tesis fueron caracterizar la expresión de losTERRA frente al estrés oxidativo, determinar los mecanismos involucrados yevaluar su relevancia en la progresión tumoral.Se observa que los TERRA se inducen a las 4 h de tratamiento con losagentes oxidantes H2O2, NaAsO2 ó butionina sulfoximina en células renalesembrionarias humanas HEK-293T. El uso del antioxidante N-acetil-L-cisteínapreviene dicho aumento. Se ha demostrado que hay aumento de ROS en el tejidoadiposo pardo de ratones expuestos al frío, pero no en el blanco. En el presentetrabajo se reporta que los TERRA se inducen sólo en el tejido adiposo pardo deratones C57BL/6J luego de 4h de ser expuestos al frío, siendo esta la primerevidencia experimental que los TERRA se inducen in vivo en una condiciónfisiológica que cursa con aumento de ROS. Por ChIP se evidenció que el H2O2induce aumento de las marcas de histonas y proteínas asociadas a activatranscripción en la región subtelomérica de las HEK-293T, donde se hallan lospromotores de los TERRA. Ensayos de RIP muestran que los TERRA interactúancon las proteínas HP1α/γ, ambas reclutadas al subtelómero. Ensayos deFinmunoprecipitación para estas proteínas sugieren que sólo HP1γ interactúa con lamaquinaria transcripcional. Se encuentra descripto que las proteínas del poronuclear (Nups) se asocian a genes transcripcionalmente activos, desconociéndoselos factores que median su interacción con la cromatina. En este trabajo seevidenciaron a las Nups presentes en complejo con HP1γ y reclutadas alsubtelómero tanto antes como después de la exposición al H2O2, sugiriendo queintervienen en la expresión de los TERRA por medio de HP1γ.La inducción de los TERRA se revierte 1-2 h luego de haber quitado el H2O2del medio de cultivo. Esta expresión dinámica de los TERRA sugiere que ejercenun rol protector. En línea con esta idea, se observa una rápida inducción deTERRAs frente a un segundo desafío con H2O2. Ensayos de ChIP sugieren queesto es posible gracias a que se conservan en la región subtelomérica las marcasepigenéticas que favorecen la transcripción luego de la primera exposicióntransitoria al H2O2, dejándolos ?preparados? para que sean transcriptos.H89, un inhibidor de la PKA, bloquea la inducción de los TERRA porexposición al H2O2, sugiriendo que PKA controla su expresión. El uso de drogasque alteran la estabilidad de los microtúbulos modula los niveles de los TERRA, aligual que ocurre cuando se cultivan células sobre superficies de diferente rigidezpara modificar la disposición del citoesqueleto. También se observa modulación deTERRAs durante la miogénesis, proceso en el que hay una reorganizacióndramática del citoesqueleto. De hecho, los rearreglos del citoesqueleto en célulasNMuMG que atraviesan la transición epitelio-mesenquimal (TEM) por tratamientocon TGFβ1, se asocian a inducción de TERRAs y a ausencia de respuesta alH2O2, tal como ocurre en las células de adenocarcinoma mamario T47D quepresentan disfunción telomérica, evidenciado por microscopía confocal. En suconjunto, los resultados aportan evidencias que los TERRA se inducen por estrésoxidativo y durante la TEM, señalando su potencial utilidad como marcadorestempranos de progresión tumoral.; Long non-coding RNAs transcribed from telomeres, known as TERRA (telomeric repeat-containing RNA), are associated with telomere and genome stability. TERRA expression is elevated in human cancer tissues, however little is known about their function. Oxidative stress damages biomolecules and activates signaling cascades involved in cell proliferation, apoptosis, and metastasis. Since telomeres are prone to oxidative damage leading to their dysfunction, the objective of the present work is to characterize TERRA expression in oxidative stress, the mechanisms involved and its relevance in EMT. H2O2 induces TERRA expression in HEK-293T cells, and is prevented by antioxidant treatment. It was reported ROS are increased in brown (BAT) but not in white adipose tissue of mice exposed to cold. Importantly, we found increased TERRAs only in BAT of mice exposed to cold. In HEK-293T cells exposed to H2O2, ChIP shows that chromatin landscape is modified favoring telomere transcription. TERRAs interact with HP1α/γ, both proteins found recruited to subtelomeres. HP1γ interacts with transcriptional machinery and nuclear pore complex proteins, suggesting they are involved in TERRA regulation. TERRA induction is lost 1-2h after removal of H2O2 from culture medium, suggesting they have protective functions. This is supported by rapid increase of TERRA upon a second H2O2 challenge. PKA inhibitor H89 blocks the increase of TERRA induced by H2O2, suggesting that PKA controls TERRA induction. Treatment of cells with drugs that disturb cytoskeleton integrity or growing cells on surfaces of different stiffness that generate differential cytoskeleton organization also modifies TERRA levels. In fact, cytoskeleton rearrangements of EMTtransformed NMuMG cells associate with higher TERRAs and lack response to H2O2, mimicking what occurs in breast cancer cells that exhibit telomere dysfunction evidenced by confocal microscopy. In summary, we show that TERRAs are induced in response to oxidative stress and during EMT, being potentially novel early markers of cancer progression.

¿La mexicanización de la Argentina? Almada, Julieta Ayelén Ensayo sobre la realidad social argentina. Se propone una comparación entre las políticas neoliberales implementadas por el gobierno de Mauricio Macri y las programáticas neoliberales mexicanas.

New record of the critically endangered striped smooth-hound, mustelus fasciatus (Garman, 1913) (chondrichthyes, triakidae), in the southwest Atlantic Cuevas, Juan Martín; Gómez, Sebastián Eduardo; Garcia, Mirta Lidia We report the southernmost known occurrence in the Atlantic Ocean of the striped smooth-hound, Mustelus fasciatus (Garman, 1913). The shark, a female measuring 106 cm TL, was fished in warm coastal waters, with a salinity of 33,189 PSU, of central Argentina (40°52ʹ13ʺ S, 062°18ʹ42ʺ W). This report increases by 205 km southward the known range of this endemic and Critically Endangered species.

Prácticas, personal político y elecciones municipales en el peronismo bonaerense (1948-1955) Marcilese, José Bernardo Este trabajo analiza la composición del personal político peronista de la provincia deBuenos Aires durante la etapa 1948-1955, a partir de un enfoque orientado aconsiderar la integración de los gobiernos locales tanto en el plano legislativo comoejecutivo. El examen se encara a partir del interés por establecer los rasgos propiosasí como también los criterios tradicionales que perduran en el peronismo almomento de determinar las candidaturas municipales.En este caso el análisis de losfuncionarios municipales conduce a pensar que perduraron principios de selecciónorientados por la notabilidad tradicional, que promovieron la integración deliderazgos locales estables.; This paper analyzes the composition of peronist political personnel in the province of Buenos Aires during the 1948-1955 period, based on an approach aimed at considering the integration of local governments in both the legislative and executive levels. The examination is addressed from the interest to establish the own characteristics as well as the traditional criteria that persist in the Peronism at the time of determining the municipal candidacies. In this case, the analysis of the municipal officials leads us to believe that selection principles guided by traditional notability persisted, which promoted the integration of stable local leaderships.

El análisis químico de las aguas: Ciencia colonial, exploración y supervivencia en península Valdés a fines del siglo XVIII; The chemical analysis of the waters: Colonial science, exploration and survival in Valdés Peninsula at the end of the 18th century Bianchi Villelli, Marcia Eliana En este trabajo presentamos una pieza documental relativa a la colonización española de la costa patagónica de fines del siglo XVIII. Es el informe sobre el análisis químico de aguas tomadas en península Valdés, durante la instalación del Fuerte San José en 1779. Se presenta la trascripción del documento inédito, seguido de las líneas de indagación que surgen del mismo: el método de análisis y “traducción” de elementos químicos mencionados. Asimismo, contextualizamos la discusión en relación con la colonización del territorio, la toma de decisiones sobre los recursos, la modernización de la química y su rol en la salud pública, en particular, la importancia del escorbuto. Esta concurrencia lleva a la notoriedad de península Valdés en el escenario colonial.; In this work we present a documentary piece related to the Spanish colonization of the Patagonian coast at the end of the 18th Century. It is the report of the chemical analysis of waters, taken in Valdés Peninsula during the installation of San José Fort in 1779. We present the transcription of an unpublished document, followed by the lines of inquiry that come up: the method of water analysis and "translation" of the chemical elements mentioned. We contextualize the discussion in relation with the colonization of the territory and decision making about resources, the modernization of chemistry and its role in public health, specifically the importance of scorbut. This concurrence leads to the notoriety of Valdés Peninsula within the colonial scenery.