The rise and fall of arbuscular mycorrhizal fungal diversity during ecosystem retrogression Kruger, Manuela; Teste, Francois; Laliberté, Etienne; Lambers, Hans; Coghlan, Megan; Zemunik, Graham; Bunce, Michael (P) limitation of primary productivity. Arbuscular mycorrhizal fungi (AMF) enhance P acquisition for most terrestrial plants, but it has been suggested that this strategy becomes less effective in strongly weathered soils with extremely low P availability. Using next generation sequencing of the large subunit ribosomal RNA gene in roots and soil, we compared the composition and diversity of AMF communities in three contrasting stages of a retrogressive >2-million-year dune chronosequence in a global biodiversity hotspot. This chronosequence shows a ~60-fold decline in total soil P concentration,with the oldest stage representing some of the most severely P-impoverishedsoils found in any terrestrial ecosystem. The richness of AMF operationaltaxonomic units was low on young (1000?s of years), moderately P-rich soils, greatest on relatively old (~120 000 years) low-P soils, and low again on the oldest (>2 000 000 years) soils that were lowest in P availability. A similar decline in AMF phylogenetic diversity on the oldest soils occurred, despite invariant host plant diversity and only small declines in host cover along the chronosequence. Differences in AMF community composition were greatest between the youngest and the two oldest soils, and this was best explained by differences in soil P concentrations. Our results point to a threshold in soil P availability during ecosystem regression below which AMF diversity declines, suggesting environmental filtering of AMF insufficiently adapted to extremely low P availability.

N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants Rossi, Fabiana Alejandra; Bochicchio, Pablo Alejandro; Quesada Allue, Luis Alberto; Pérez, Martín Mariano Drosophila melanogaster Meigen mutants for N-β-alanyldopamine (NBAD) metabolism have altered levels of NBAD, dopamine and other neurotransmitters. The ebony1 mutant strain has very low levels of NBAD and higher levels of dopamine, whereas the opposite situation is observed in the tan1 mutant. Dopamine is implicated in the control of movement, memory and arousal, as well as in the regulation of sleep and wakefulness in D. melanogaster. N-β-alanyldopamine, which is best known as a cuticle cross-linking agent, is also present in nervous tissue and has been proposed to promote locomotor activity in this fly. The daily locomotor activity and the sleep patterns of ebony1 and tan1 mutants are analyzed, and are compared with wild-type flies. The tan1 mutant shows reduced locomotor activity, whereas ebony1 shows higher levels of activity than wild-type flies, suggesting that NBAD does not promote locomotor activity. Both mutants spend less time asleep than wild-type flies during night-time; ebony shows more consolidated activity during night-time and increased sleep latency, whereas tan is unable to consolidate locomotor activity and sleep in either phase of the day. The daily level of NBAD-synthase activity is measured in vitro using wild-type and tan1 protein extracts, and the lowest NBAD synthesis is observed at the time of higher locomotor activity. The abnormalities in several parameters of the waking/sleep cycle indicate some dysfunction in the processes that regulates these behaviours in both mutants.

Efecto de vecino: el poder de Brasil sobre el regionalismo de Argentina; The neighbouring effect: the power of Brazil over Argentinian regionalism Miranda, Roberto Alfredo El objetivo de este trabajo es analizar las características de la relación de Argentina con Brasil, a fin de determinar el efecto que este bilateralismo le provocó a su política exterior. El análisis, centrado en la primera década del presente siglo, tiene como eje las consecuencias de la situación de dependencia comercial de Argentina con Brasil. Sobre esta situación sostenemos que para Argentina tuvo un costo político significativo. Para desarrollar esta perspectiva pasamos revista a las relaciones bilaterales entre ambos países, fundamentalmente en lo concerniente a las coincidencias y divergencias que tuvieron en torno al Mercosur. Al mismo tiempo consideramos, como por un lado, las implicancias del liderazgo brasileño, y por el otro, las alternativas de política exterior que manejó Argentina para tratar el vínculo con su vecino desde un lugar diferente. Entre las conclusiones a las que arribamos, destacamos que la dependencia comercial con Brasil empujó a Argentina a secundar políticamente a su vecino en la estructuración de la integración sudamericana.; The purpose of this paper is to analyse the characteristics of the relation between Argentina and Brazil in order to determine the effect that this bilateralism provoked to its foreign policy. The analysis, centred in the first decade of this century, focuses on the consequences of the commercial dependence that Argentina has with Brazil. Concerning this situation we believe that Argentina had a significant political cost. In order to develop this point of view we go through the bilateral relations between both countries, especially in what is concerned with the coincidences and divergences that they have as regards Mercosur. At the same time we consider on one hand the implications of Brazilian leadership and on the other the alternatives of foreign policy that Argentina implemented to deal with her neighbour from a different place. Among the conclusions we arrived to, we highlight the commercial dependence with Brazil that led Argentina to follow its neighbour model in the building of South American integration.

Non est porro tuum uno sensu solum oculorum moueri: consolatio y visibilidad en las cartas de Cicerón a Marcelo; Non est porro tuum uno sensu solum oculorum moueri: consolatio and visibility in Ciceroʹs letters to Marcellus Correa, Soledad Este artículo pretende mostrar la forma estratégica en que usó Cicerón la carta de consuelo para ganar visibilidad y proteger su imagen durante un período crítico de su vida política. Como la renuencia de Marcelo a aceptar la clementia de César pone en riesgo esta estrategia, Cicerón activará la dimensión exhortativa del discurso de consuelo con la intención de negociar su propia imagen social y su rumbo de acción en comparación con la de su destinatario.; This article seeks to show how Cicero strategically used the consolatory letter in order to gain visibility and save his face during a critical period of his political life. As Marcellus reluctance to accept Caesar’s clementia places this strategy in jeopardy, Cicero will activate the exhortative dimension of consolatory discourse in order to nego‐ tiate his own social image and his course of action in comparison with his addressee’s.; Este artigo pretende mostrar como Cícero usou estrategicamente a carta de consolação para ganhar visibilidade e salvar a face durante um período crítico da sua vida política. Como a relutância de Marcelo em aceitar a clementia de César coloca esta estratégia em perigo, Cícero ativará a dimensão exortativa do discurso de consolação com vista a negociar a sua própria imagem social e o seu curso de ação em comparação com a do seu destinatário.; Cet article prétend montrer comment Cicéron a stratégiquement utilisé la lettre de consolation pour gagner de la visibilité et sauver la face durant une période critique de sa vie politique. Comme le doute de Marcellus à accepter la clementia de César met cette stratégie en danger, Cicéron activera la dimension exhortative du discours de consolation, dans le but de négocier sa propre image sociale et son discours d’action, en comparaison avec celui de son destinataire.

Salud percibida y rendimiento académico en adolescentes de escuelas públicas de la ciudad de Córdoba; Perceived health and academic performance among adolescents from public schools in the city of Córdoba Vitale, Romina; Degoy, Emilse; Berra, Silvina del Valle Introducción: El desempeño en la escuela podría estar vinculado a la salud en la adolescencia, y los logros educativos en esta edad pueden influir en la vida futura. El objetivo fue estudiar la asociación entre el rendimiento académico y la salud percibida en adolescentes, teniendo en cuenta las características sociodemográficas de sus familias. Población y métodos: Estudio piloto transversal en una muestra de adolescentes asistentes al ciclo básico de tres escuelas secundarias públicas de la ciudad de Córdoba, Argentina. El rendimiento académico se computó como el promedio de las calificaciones en todas las asignaturas y se consideró suficiente cuando fue igual a 6 puntos o mayor. La salud percibida se valoró con el cuestionario KIDSCREEN-52, que produce puntuaciones en diez dimensiones. Además, se recogió la edad, el sexo, el nivel de escolaridad materna, el nivel socioeconómico y la composición de la familia. Se realizaron análisis univariados, bivariados y modelos multivariados de regresión logística. Resultados: Participaron 554 adolescentes, 52% de los cuales eran mujeres. El rendimiento académico insuficiente (27,6%) fue más frecuente en los adolescentes que manifestaron peor relación con los padres (OR 2,68; IC 95%: 1,22- 5,85) y mejor relación con amigos (OR 0,46; IC 95%: 0,26-0,82). Hubo diferencias al estratificar por nivel socioeconómico: en el nivel alto, el rendimiento insuficiente fue más frecuente en adolescentes con percepción de menor autonomía y, en el nivel medio-bajo, en adolescentes con peor percepción del entorno escolar. Conclusión: El rendimiento académico se asoció a dimensiones psicosociales de la salud, como sus relaciones familiares, con amigos, autonomía y entorno escolar.

Expression of conditioned preference for low-quality food in sheep is modulated by foraging cost Catanese, Francisco Hernan; Distel, Roberto Alejandro; Villalba, Juan Jose Past positive experiences can increase herbivores’ motivation to eat low-quality foods. However, this is not always translated into a higher preference for low-quality foods in choice tests among foods of higher nutritional quality. Foraging behavior is also affected by properties of the feeding context because the quality and abundance of foods in nature change in time and space. We hypothesized that in a choice situation, the expression of a past positive experience with a low-quality food is modulated by the costs associated with selecting a high-quality food option. A total of 24 sheep were randomly assigned into two groups (n = 12). During conditioning phase, one group (CS+; i.e., conditioned group) was fed with oat hay (a low-quality food) for 20 min and immediately after a ration of soybean meal (a nutritious food), whereas the other group was also fed with oat hay but the offer of soybean meal was delayed 5 h (CS−; i.e., control group). After conditioning, we assessed sheep motivation to eat the oat hay in an experimental arena in which accessibility to alfalfa hay (a high-quality food) was increasingly restricted. When alfalfa hay was readily accessible, CS+ and CS− sheep almost exclusively selected this food, showing a small and similar preference for oat hay. However, when accessibility to alfalfa hay decreased, intake and selection of oat hay was greater in the CS+ sheep than in the CS− sheep. The latter was a consequence of differential changes in behavior between groups; for example, sheep in CS+ spent more time foraging oat hay and were more likely to switch to oat hay if they had previously been eating alfalfa hay than sheep in CS−. Our results show that behavioral expression of the conditioned preference for a low-quality food depends on parameters of the feeding context (e.g., availability). We suggest that this can be the link between learning models and optimal foraging models of diet selection.

Sol gel hybrid coatings with strontium-doped 45S5 glass particles for enhancing the performance of stainless steel implants: Electrochemical, bioactive and in vivo response Omar, Sheila Ayelén; Repp, Felix; Desimone, Paula Mariela; Weinkamer, Richard; Wagermaier, Wolfgang; Ceré, Silvia; Ballarre, Josefina The protection of stainless-steel implants by applying a hybrid organic–inorganic coating generates a barrier for ion migration and a potential holder for functional particles. Chemical composition of bioactive silicate-glasses (BG) can be varied to tailor their rate of dissolution in the biological environment. The substitution of calcium by strontium (Sr) generates a locally-controlled release of Sr-ions to the media. Strontium is known to reduce bone resorption and stimulate bone formation. This work presents coatings made by sol–gel method containing tetraethoxysilane, methyl-triethoxysilane and silica nanoparticles as precursors, and functionalized either with BG or Sr-substituted BG particles onto surgical grade stainless steel. The coated implants were tested in vitro for corrosion resistance and bioactivity, and in vivo to analyze bone formation. The applied coating system provided an excellent protection to aggressive fluids, even after 30 days of immersion. The presence of hydroxyapatite is shown as a first evidence of bioactivity. The evaluation of in vivo tests in Wistar–Hokkaido rat femur 4 or 8 weeks after the implantation showed slight differences in the thickness of newly formed bone measured by ESEM, and remarkable changes in bone quality characterized with Raman microscopy. The in vivo response of the coatings containing Sr-substituted bioglass is better at early times of implantation as regards the bone morphology and quality making this functionalized coatings a very promising option for implant protection and bone regeneration.

Contaminants in the southern tip of South America: Analysis of organochlorine compounds in feathers of avian scavengers from Argentinean Patagonia Martínez López, Emma; Espín, Silvia; Barbar, Facundo; Lambertucci, Sergio Agustin; Gómez Ramírez, Pilar; García Fernández, A. J. The aim of this study was to assess the exposure to organochlorine compounds (OC) in 91 primary wing feathers of avian scavengers, Turkey vulture (Cathartes aura), American black vulture (Coragyps atratus) and Southern crested caracaras (Polyborus plancus) from the southern tip of South America, in the Argentinean Patagonia. We analyzed for a series of OC including hexachlorocyclohexane (HCH) isomers, endosulfan, aldrin, dieldrin, endrin, dichlorodiphenyltrichloroethane (p,p’-DDT), dichlorodiphenyldichloroethane (p,p’-DDD), dichlorodiphenyldichloroethylene (p,p’-DDE), heptachlor and heptachlor-epoxide. This is the first study on OC in feathers of three terrestrial top carnivores from South America. OC concentrations found in the studied species were much higher than those found in feathers of raptors from Europe and Asia, which likely indicate their high use in the region, specifically in agriculture, and other possible uses of OC in this area. ∑HCH had the highest median concentration, followed by ∑Drins, ∑DDT, ∑Heptachlor, and ∑Endosulfan, similar to those reported in several food samples in Argentina. On the other hand, differences in OC profiles between species and areas may be related to feeding and migratory habits, as well as the molt period. Three individuals showed ∑DDT (DDT, DDD and DDE) concentrations in feathers related to sublethal effects. However, this comparison should be used with caution due to problems with extrapolating such data across tissues and species.

Administration of caseinomacropeptide-enriched extract to mice enhances the calcium content of femur in a low-calcium diet Burns, Patricia Graciela; Binetti, Ana Griselda; Torti, Pablo; Kulozik, U.; Forzani, Liliana Maria; Renzulli, P.; Vinderola, Celso Gabriel; Reinheimer, Jorge Alberto Caseinomacropeptide (CMP) is the 64C-terminal amino acid residue of κ-casein, formed by chymosin cleavage during cheese manufacture. This study examined the effects of oral administration of a CMP-enriched extract (CMPEE), obtained from a local dairy plant, on the Ca content of mouse femurs. Animals received low (0.1%, w/v), normal (0.5%, w/v) or high (1.2%, w/v) Ca diet for 3 or 8 weeks and CMPEE diluted (1:10) in their drinking water. No significant differences in Ca content were observed in faeces, kidney, urine or blood serum compared with control animals. The oral administration of CMP to mice significantly enhanced the Ca content in femur under a low-Ca diet model, especially during the period of full body development (3 weeks), in which case a significant 12% Ca increase was observed. These findings pave the way for further studies aimed at supplementing infant food with industrially-obtained CMP-enriched extract for enhanced bone health.

Comparing generic models for interplanetary shocks and magnetic clouds axis configurations at 1 AU Janvier, Miho; Dasso, Sergio Ricardo; Démoulin, Pascal; Masías Meza, Jimmy Joel; Lugaz, Noé Interplanetary coronal mass ejections (ICMEs) are the manifestation of solar transient eruptions, which can significantly modify the plasma and magnetic conditions in the heliosphere. They are often preceded by a shock, and a magnetic flux rope is detected in situ in a third to half of them. The main aim of this study is to obtain the best quantitative shape for the flux rope axis and for the shock surface from in situ data obtained during spacecraft crossings of these structures. We first compare the orientation of the flux rope axes and shock normals obtained from independent data analyses of the same events, observed in situ at 1 AU from the Sun. Then we carry out an original statistical analysis of axes/shock normals by deriving the statistical distributions of their orientations. We fit the observed distributions using the distributions derived from several synthetic models describing these shapes. We show that the distributions of axis/shock orientations are very sensitive to their respective shape. One classical model, used to analyze interplanetary imager data, is incompatible with the in situ data. Two other models are introduced, for which the results for axis and shock normals lead to very similar shapes; the fact that the data for MCs and shocks are independent strengthens this result. The model which best fits all the data sets has an ellipsoidal shape with similar aspect ratio values for all the data sets. These derived shapes for the flux rope axis and shock surface have several potential applications. First, these shapes can be used to construct a consistent ICME model. Second, these generic shapes can be used to develop a quantitative model to analyze imager data, as well as constraining the output of numerical simulations of ICMEs. Finally, they will have implications for space weather forecasting, in particular, for forecasting the time arrival of ICMEs at the Earth.

Microwave-assisted synthesis of novel pyrazolo[3,4-g][1,8]naphthyridin-5-amine with potential antifungal and antitumor activity Acosta, Paola; Butassi, Estefanía; Insuasty, Braulio; Ortiz, Alejandro; Abonia, Rodrigo; Zacchino, Susana Alicia Stella; Quiroga, Jairo The microwave assisted reaction between heterocyclic o-aminonitriles 1 and cyclic ketones 2 catalyzed by zinc chloride led to new series of pyrazolo[3,4-b] [1,8]naphthyridin-5-amines 3 in good yields. This procedure provides several advantages such as being environmentally friendly, high yields, simple work-up procedure, broad scope of applicability and the protocol provides an alternative for the synthesis of pyrazolonaphthyridines. The whole series showed antifungal activities against Candida albicans and Cryptococcus neoformans standardized strains, being compounds with a 4-p-tolyl substituent of the naphthyridin scheleton (3a, 3d and 3g), the most active ones mainly against C. albicans, which appear to be related to their comparative hydrophobicity. Among them, 3d, containing a cyclohexyl fused ring, showed the best activity. The anti-Candida activity was corroborated by testing the three most active compounds against clinical isolates of albicans and non-albicans Candida strains. These compounds were also screened by the US National Cancer Institute (NCI) for their ability to inhibit 60 different human tumor cell lines. Compounds 3a and 3e showed remarkable antitumor activity against cancer cell lines, with the most important GI50 values ranging from 0.62 to 2.18 μM.

Las brechas territoriales del desarrollo argentino: Un balance (crítico) de los años 2000 Niembro, Andrés Alberto El problema de las desigualdades regionales de desarrollo es un tema vigente en Argentina. En general, los enfoques y las metodologías aplicadas hasta ahora suelen limitarse a abordar las disparidades territoriales a partir de la evaluación de únicamente unas pocas variables o dimensiones del desarrollo. Esta investigación busca aportar una nueva forma de dimensionar y cuantificar las brechas internas del desarrollo argentino, desde un abordaje más amplio de las diferentes facetas del proceso. La aplicación de la metodología propuesta nos arroja un balance más bien crítico para los años 2000. Luego de una década atravesada por el crecimiento económico y la recomposición de los indicadores sociales post-crisis de 2001-2002, las disparidades provinciales tendieron a ensancharse en la mayor parte de los campos del desarrollo estudiados, lo cual sugiere que las mejoras no se habrían distribuido de forma equitativa a lo largo y ancho del territorio nacional.

Experimental investigation of wall nucleation characteristics in flow boiling Brooks, Caleb S.; Silin, Nicolas; Hibiki, Takashi; Ishii, Mamoru Wall nucleation experiments have been performed in a vertical annulus test section for investigation of the bubble departure diameter and bubble departure frequency. The experimental data in forced convective subcooled boiling flow is presented as a parametric study of the effect of wall heat flux, local bulk liquid subcooling, liquid flow rate, and system pressure. The data are shown to extend the database currently available in literature to a wider range of system conditions. Along with the current database in forced convective flow, the available models for bubble departure size and frequency are reviewed and compared with the existing database. The prediction of the bubble departure frequency is shown to require accurate modeling of the bubble departure diameter which has poor agreement with the experimental database.

Land use and land cover change impacts on the regional climate of non-Amazonian South America: A review Salazar, Alvaro; Baldi, Germán; Hirota, Marina; Syktus, Jozef; McAlpine, Clive Land use and land cover change (LUCC) affects regional climate through modifications in the water balance and energy budget. These impacts are frequently expressed by: changes in the amount and frequency of precipitation and alteration of surface temperatures. In South America, most of the studies of the effects of LUCC on the local and regional climate have focused on the Amazon region (54 studies), whereas LUCC within non-Amazonian regions have been largely undermined regardless their potential importance in regulating the regional climate (19 studies). We estimated that 3.6million km^2 of the original natural vegetation cover in non-Amazonian South America were converted into other types of land use, which is about 4 times greater than the historical Amazon deforestation. Moreover, there is evidence showing that LUCC within such fairly neglected ecosystems cause significant reductions in precipitation and increases in surface temperatures, with occasional impacts affecting neighboring or remote areas. We explore the implications of these findings in the context of water security, climatic extremes and future research priorities.

La viviendas social y la cuestión urbana. Consideraciones teóricas para el análisis de las políticas de hábitat; Social housing and the urban matter.Thoughts on the analysis of housing policies del Río, Juan Pablo Este artículo tiene por objetivo explorar las relaciones entre hábitat popular y ciudad a efectos de aportar algunos elementos teóricos para el estudio de las políticas públicas de hábitat. Para ello se realiza un análisis bibliográfico de los debates clásicos del problema de la vivienda, la cuestión urbana y las teorías de la ciudad latinoamericana, a partir del cual se postula que las contradicciones inherentes enacceso al espacio urbano no son ajenas a la tensión capital-trabajo. En paralelo se afirma que, en elcontexto latinoamericano, el estudio de la dinámica de los mercados de suelo ha sido relegado comovariable explicativa de este problema. Además, se considera necesario de-construir el discurso«viviendista» o sectorial, que aun forma parte de la ideología dominante de la política habitacional argentina. Y se sostiene que la noción de localización es una clave de lectura importante para interpelar las políticas de hábitat. Finalmente, arribamos a la conclusión que una de las prioridades de la política en relación a la cuestión del hábitat debería ser la regulación de los mercados inmobiliarios y el castigo a la especulación urbana.; his article aims to explore the inter-relations between habitat and city in order to provide some theoreticalinsights for the study of habitat-related public policies. For that reason a review of the literature on theclassical debates is carried out regarding the problem of housing, urban issues, and theories of the LatinAmerican city, after which it is postulated that the contradictions inherent to the access to urban space are not alien to the capital-labor tension. It is stated that in the Latin American context, the study of the dynamicsof land markets has been neglected as an explanatory variable of this problem. In addition, it is necessaryto deconstruct the «viviendista» or sectorial discourse, part of the dominant ideology of housing policy. Itis also argued that the notion of localization is an important perspective to assess habitat policies. Finally,we arrive at the conclusion that one of the main priorities of the policy in relation to the issue of habitatshould be the real estate market regulation and the punishment of urban speculation.

La aventura colectiva de mi feminismo. Apuntes sobre las experiencias de lxs estudiantes de Letras de la Universidad de Buenos Aires en torno a los estudios de género Angilletta, María Florencia Hay algo hermoso, inquietante, por momentos perturbador: resulta difícil encontrar dos licenciadxs en Letras, egresadxs de la Facultad de Filosofía y Letras (FFyL) de la Universidad de Buenos Aires (UBA), que hayan cursado exactamente la misma carrera. Incluso entre estudiantes que hayan seguido la misma orientación es poco probable que hayan asistido a los mismos seminarios, o incluso que hayan estudiado los mismos programas si cursaron iguales materias. Más allá de los debates abiertos sobre el plan de estudios y sobre los efectos “elige tu propia aventura”, aquí se plantea que una cursada en Letras es también una generación, un clima, por qué no una moda, una elección, una política. Cursé la Licenciatura y el Profesorado en Letras entre 2007 y 2013. Mi épica personal se fricciona con una hipótesis colectiva, con un ciclo abierto en 2003, que ha esbozado un nuevo país para ser joven, un nuevo ámbito de lo público, un nuevo escenario de profesionalización del docente investigador. En especial, fueron nuevos años –felices– para ser feminista.

Ninjas, princesas y robots gigantes: género, formato y contenido en el manganime González Torrents, Alba Este artículo se sirve de las nociones veronianas de género-L y género-P para intentar aportar un poco de luz a la problemática de la clasificación de géneros en el manga y el anime. Para ello, se adopta estratégicamente la distinción entre género-L y género-P, aplicándola al manga y al anime, para mostrar a continuación que la aplicación de dichas categorías veronianas a este campo de producción cultural japonés es mucho más problemática de lo que puede parecer en un principio.; This article uses Veronian notions of L-genre and P-genre to try to bring some light to the problem of the classification of genres in manga and anime. The essay strategically adopts the distinction between L-genre and P-genre, applying it to manga and anime, to eventually show how the application of these Veronian categories to this field of Japanese cultural production is much more problematic than it may seem at first.

Literatura y política. Cómo leer el antiperonismo de Cortázar; Literature and Politics. How to read Cortazar's anti-Peronism Demarchi, Rogelio Gabriel La lectura del antiperonismo en la obra de Cortázar fue sugerida por Juan José Sebreli y ampliada por Andrés Avellaneda, pero puesta en duda o reconfigurada por David Viñas, Ricardo Piglia y Carlos Gamerro, entre otros. La propuesta de este artículo es volver sobre la cuestión a través de una serie literaria no restringida a la primera parte de su obra, como suele ser habitual en la discusión, sino con una ampliada hasta Libro de Manuel. Así se advierte, primero, que, en un importante segmento de sus ficciones, el antiperonismo de los primeros textos no desapareció sino que se enmascaró y se volvió de difícil lectura por un efecto combinado de su compromiso con la Revolución Cubana y su crítica a la pequeña burguesía; y, segundo, que resurge en su novela política de los 70.

Synthesis, characterization, X-ray crystal structure, DFT calculation, DNA binding, and antimicrobial assays of two new mixed-ligand copper(II) complexes Ebrahimipour, S. Yousef; Sheikhshoaie, Iran; Mohamadi, Maryam; Suarez, Sebastian; Baggio, Ricardo Fortunato; Khaleghi, Moj; Torkzadeh Mahani, Masoud; Mostafavi, Ali Two new Cu(II) complexes, [Cu(L)(phen)] (1), [Cu(L)(bipy)] (2), where L2- = (3-methoxy-2oxidobenzylidene)benzohydrazidato, phen = 1,10 phenanthroline, and bipy = 2,2′ bipyridine, were prepared and fully characterized using elemental analyses, FT-IR, molar conductivity, and electronic spectra. The structures of both complexes were also determined by X-ray diffraction. It was found that, both complexes possessed square pyramidal coordination environment in which, Cu(II) ions were coordinated by donor atoms of HL and two nitrogens of heterocyclic bases. Computational studies were performed using DFT calculations at B3LYP/6-311+G(d,p) level of theory. DNA binding activities of these complexes were also investigated using electronic absorption, competitive fluorescence titration and cyclic voltammetry studies. The obtained results indicated that binding of the complexes to DNA was of intercalative mode. Furthermore, antimicrobial activities of these compounds were screened against microorganisms.

Serosurvey of leptospira interrogans, brucella abortus and chlamydophila abortus infection in free-ranging giant anteaters (myrmecophaga tridactyla) from Brazil; Inquérito sorológico de Leptospira interrogans, Brucella abortus e Chlamydophila abortus em tamanduás-bandeira (Myrmecophaga tridactyla) de vida livre no Brasil Miranda, Flávia; Superina, Mariella; Vinci, Fernanda; Hashimoto, Vanessa; Freitas, Julio César; Matushima, Eliana Reiko A serological survey for antibodies against Leptospira interrogans, Brucella abortus, and Chlamydophila abortus was conducted in 21 clinically healthy, free-ranging giant anteaters (Myrmecophaga tridactyla) from Parque Nacional das Emas (Goiás State, Brazil; n=6), Parque Nacional da Serra da Canastra (Minas Gerais State, Brazil; n=9), and RPPN SESC Pantanal (Mato Grosso State, Brazil; n=6) between July 2001 and September 2006. Sera were screened for antibodies against 22 serovars of Leptospira interrogans with a microscopic agglutination test. Twelve tested positive for L. interrogans serovars sentot (n=5 in PN Emas, n=2 in PN Serra da Canastra), butembo (n=2 in PN Serra da Canastra), autumnalis, bataviae, and shermani/icterohaemorrhagiae (n=1 each in SESC Pantanal). One adult female tested positive for B. abortus with the buffered plate antigen test. All sera were negative for C. abortus using the complement fixation text. This is the first report of pathogens that may interfere with the reproduction and population dynamics of free-ranging giant anteaters.; Inquéritos sorológicos para detecção de anticorpos contra Leptospira interrogans, Brucella abortus, e Chlamydophila abortus foram realizados em 21 tamanduás-bandeira (Myrmecophaga tridactyla) de vida livre do Parque Nacional das Emas (Goiás, Brasil, n=6), o Parque Nacional da Serra da Canastra (Minas Gerais, Brasil, n=9) e RPPN SESC Pantanal (Mato Grosso, Brasil, n=6) entre julho de 2001 e setembro de 2006. Os sor os foram testados para anticorpos contra 22 sorotipos de Leptospira interrogans com um teste de aglutinação microscópica. Doze animais foram considerados positivos para L. interrogans sorovares sentot (n=5 em PN Emas, n=2 em PN Serra da Canastra), butembo (n=2 em PN Serra da Canastra), autumnalis, bataviae e shermani/icterohaemorrhagiae(n=1 para cada sorovar em SESC Pantanal). Uma fêmea adulta testou positivo para B. abortuscom o teste do antígeno tamponado. Todos os soros se mostraram negativos para C. abortusatravés do teste de fixação do complemento. Este é o primeiro relato de patógenos que podem interferir na dinâmica reprodutiva de populações de tamanduás em estado selvagem.