CLCA2 epigenetic regulation by CTBP1, HDACs, ZEB1, EP300 and miR-196b-5p impacts prostate cancer cell adhesion and EMT in metabolic syndrome disease Porretti, Juliana Carla; Dalton, Guillermo Nicolás; Massillo, Cintia Lorena; Scalise, Georgina Daniela; Farré, Paula Lucía; Elble, Randolph; Gerez, Esther Noemi; Accialini, Paula Lucia; Cabanillas, Ana Maria de Los A.; Gardner, Kevin; de Luca, Paola; de Siervi, Adriana Prostate cancer (PCa) is the most common cancer among men. Metabolic syndrome (MeS) is associated with increased PCa aggressiveness and recurrence. Previously, we proposed C-terminal binding protein 1 (CTBP1), a transcriptional co-repressor, as a molecular link between these two conditions. Notably, CTBP1 depletion decreased PCa growth in MeS mice. The aim of this study was to investigate the molecular mechanisms that explain the link between MeS and PCa mediated by CTBP1. We found that CTBP1 repressed chloride channel accessory 2 (CLCA2) expression in prostate xenografts developed in MeS animals. CTBP1 bound to CLCA2 promoter and repressed its transcription and promoter activity in PCa cell lines. Furthermore, we found that CTBP1 formed a repressor complex with ZEB1, EP300 and HDACs that modulates the CLCA2 promoter activity. CLCA2 promoted PCa cell adhesion inhibiting epithelial–mesenchymal transition (EMT) and activating CTNNB1 together with epithelial marker (CDH1) induction, and mesenchymal markers (SNAI2 and TWIST1) repression. Moreover, CLCA2 depletion in PCa cells injected subcutaneously in MeS mice increased the circulating tumor cells foci compared to control. A microRNA (miRNA) expression microarray from PCa xenografts developed in MeS mice, showed 21 miRNAs modulated by CTBP1 involved in angiogenesis, extracellular matrix organization, focal adhesion and adherents junctions, among others. We found that miR-196b-5p directly targets CLCA2 by cloning CLCA2 3′UTR and performing reporter assays. Altogether, we identified a new molecular mechanism to explain PCa and MeS link based on CLCA2 repression by CTBP1 and miR-196b-5p molecules that might act as key factors in the progression onset of this disease.

Spatial and temporal plant-to-plant variability effects on soybean yield Masino, Alejandra; Rugeroni, Pablo; Borras, Lucas; Rotundo, José Luis Plant density and row spacing are management practices that farmers use to maximize soybean (Glycine max (L.) Merr.) yield. Other canopy aspects related to seedling establishment like spatial (spacing among plants) and temporal (timing of plant emergence) within-row plant-to-plant variability have received less attention. Negative yield effects of non-uniform stands have been reported for maize (Zea mays subsp. Mays L.), but it is commonly accepted that soybean plants compensate for any plant-to-plant growth difference. Planting quality is becoming relevant because small yield effects can have a high economic return due to high soybean prices and low implementation costs. The objectives were to assess the impact of non-uniform spatial and temporal seedling establishment on soybean yields and to identify putative mechanisms. Results showed that, across planting dates and plant densities, there was no yield loss due to increased spatial plant-to-plant variation in a later maturity group cultivar (MG IV), but reduced yield in an earlier one (MG III). Contrarily, non-uniform temporal distributions significantly reduced yield across cultivars. Having a poor spatial distribution had no effect on the average plant growth rate or its plant-to-plant variability. A poor temporal distribution did not affect individual average plant growth rate but variability was increased. This increased variability determined that some individuals had higher plant growth but reduced seed number because of reduced reproductive partitioning. Novel findings can be summarized as: (i) the higher relative importance of temporal vs. spatial non-uniform canopies in determining soybean yield reductions, (ii) the influence of reduced seed set at higher plant growth rates to mechanistically explain yield reductions in non-uniform temporal canopies; (iii) the concept that the yield effect of non-uniform temporal canopies cannot be overcome by increased plant densities. Future research needs to better understand the interactions between cultivar reproductive characteristics and susceptibility to non-uniform temporal canopies.

Molecular confirmation of the linkage between the rhizopus oryzae CYP51A gene coding region and its intrinsic voriconazole and fluconazole resistance Macedo, Daiana; Leonardelli, Florencia; Dudiuk, Catiana Beatriz; Theill, Laura; Cabeza, Matías Sebastián; Gamarra, Soledad; Garcia, Guillermo Manuel Rhizopus oryzae is the most prevalent causative agent of mucormycosis, an increasingly reported opportunistic fungal infection. These Mucorales are intrinsically resistant to Candida- and Aspergillus-active antifungal azole drugs, such as fluconazole (FLC) and voriconazole, respectively. Despite its importance, the molecular mechanisms of its intrinsic azole resistance have not been elucidated yet. The aim of this work was to establish if the Rhizopus oryzae CYP51 genes are uniquely responsible for intrinsic voriconazole and fluconazole resistance in these fungal pathogens. Two CYP51 genes were identified in the R. oryzae genome. We classified them as CYP51A and CYP51B based on their sequence similarity with other known fungal CYP51 genes. Later, we obtained a chimeric Aspergillus fumigatus strain harboring a functional R. oryzae CYP51A gene expressed under the regulation of the wild-type A. fumigatus CYP51A promoter and terminator. The mutant was selected after transformation by using a novel procedure taking advantage of the FLC hypersusceptibility of the A. fumigatus CYP51A deletion mutant used as the recipient strain. The azole susceptibility patterns of the A. fumigatus transformants harboring R. oryzae CYP51A mimicked exactly the azole susceptibility patterns of this mucormycete. The data presented in this work demonstrate that the R. oryzae CYP51A coding sequence is uniquely responsible for the R. oryzae azole susceptibility patterns.

El concepto de capitalismo en la perspectiva pragmática de Boltanski y Chiapello; The Concept of Capitalism in Boltanski and Chiapello’s Pragmatic Perspective Gonnet, Juan Pablo; Abril, Francisco Manuel En el presente trabajo proponemos un análisis crítico de la conceptualización del capitalismo ofrecida por la perspectiva pragmática de Boltanski y Chiapello en su libro El nuevo espíritu del capitalismo. Para estos autores el capitalismo es la exigencia de acumulación ilimitada de capital que se legitima mediante justificaciones morales, reconociéndose un vínculo ineludible entre el capitalismo y la moral. Nuestro objetivo es mostrar que el mismo es interpretado de modos variables, ambiguos y contradictorios, lo que genera una teorización imprecisa en torno al capitalismo y a los aportes que la corriente sociológica del pragmatismo podría brindar para su comprensión.; This article critically examines the pragmatic conceptualization of capitalism offered in Boltanski and Chiapello’s book El nuevo espíritu del capitalismo (The New Spirit of Capitalism). For them, capitalism is the demand for unlimited accumulation of capital legitimized through moral justifications, thus recognizing an unavoidable link between capitalism and morality. The article’s authors aim to show that this is interpreted in different ways that are both ambiguous and contradictory, thus generating an imprecise theorization of capitalism and the contributions that the pragmatist sociological current can offer for understanding it.

¿Centros o cuerpos de delegados?: Las luchas estudiantiles de los años setenta frente al debate acerca de las formas organizativas. El caso de la UBA; Student centers or bodies o delegates?: The student struggles of the seventy years against the debate about the organizational forms. The case of the UBA Califa, Juan Sebastián En este trabajo se dará cuenta de las pujas generadas a comienzos de los años setenta en el movimiento estudiantil argentino en lo concerniente a los principios organizativos más eficaces para derrotar a la dictadura imperante. Mientras que el reformismo bregaba por reconstruir los centros de estudiantes, otras organizaciones de izquierda y en menor medida peronistas se orientaban a fortalecer los cuerpos de delegados por curso. Los avatares de esta competencia, con sus ganadores y perdedores, permite adentrarse en un período clave de la universidad argentina, revisitando así el debate acerca de la vigencia del reformismo como identidad programática entre sus capas más jóvenes. Este artículo, se adentrará en lo acaecido en la Universidad de Buenos Aires (UBA), acudiendo a fuentes y bibliografía variadas.; In this paper, we will see the bids generated in the early seventies in the Argentine student movement regarding the most effective organizing principles to defeat the ruling dictatorship. While reformism struggled to rebuild student centers, other organizations of the left and, to a lesser extent, Peronists aimed to strengthen the bodies of delegates per course. The ups and downs of this competition, with its winners and losers, allows us to enter a key period of the Argentine university, revisiting the debate about the validity of reformism as a programmatic identity among its younger layers. This article will delve into what happened at the University of Buenos Aires (UBA), going to sources and bibliography varied.

Categorías espaciales y dicotomías jerárquicas en la definición de la santidad femenina de María Egipciaca; Spatial Categories and Hierarchical Dichotomies in the Definition of the Female Sanctity of Mary of Egypt Zubillaga, Carina Alejandra En la "Vida de Santa María Egipciaca", poema hispánico de la primera mitad del siglo XIII transmitido por el códice K-III-4 de la Biblioteca de San Lorenzo de El Escorial del siglo XIV, las categorías espaciales exterior-interior, margen-centro, bajo-alto permiten ahondar en la santidad femenina del período como práctica social, a partir de la configuración de la leyenda de esta figura paradigmática de las prostitutas arrepentidas. En el presente artículo, se ponen de relieve los espacios comunitarios del templo y el monasterio frente al espaciodesolado del desierto, representativo de una de las variantes de la marginalidad de la espiritualidad femenina en el Medioevo.

Interaction of gabaergic ketones with model membranes: A molecular dynamics and experimental approach Miguel, Virginia; Sánchez, Mariela Eugenia; Garcia, Daniel Asmed γ-Aminobutyric-acid receptor (GABA A -R), a membrane intrinsic protein, is activated by GABA and modulated by a wide variety of recognized drugs. GABA A -R is also target for several insecticides which act by recognition of a non-competitive blocking site. Mentha oil is rich in several ketones with established activity against various insects/pests. Considering that mint ketones are highly lipophilic, their action mechanism could involve, at least in part, a non-specific receptor modulation by interacting with the surrounding lipids. In the present work, we studied in detail the effect on membranes of five cyclic ketones present in mint plants, with demonstrated insecticide and gabaergic activity. Particularly, we have explored their effect on the organization and dynamics of the membrane, by using Molecular Dynamics (MD) Simulation studies in a bilayer model of DPPC. We performed free diffusion MD and obtained spatially resolved free energy profiles of ketones partition into bilayers based on umbrella sampling. The most favored location of ketones in the membrane corresponded to the lower region of the carbonyl groups. Both hydrocarbon chains were slightly affected by the presence of ketones, presenting an ordering effect for the methylene groups closer to the carbonyl. MD simulations results were also contrasted with experimental data from fluorescence anisotropy studies which evaluate changes in membrane fluidity. In agreement, these assays indicated that the presence of ketones between lipid molecules induced an enhancement of the intermolecular interaction, increasing the molecular order throughout the bilayer thickness.

Beta-Glucan and Phenolic Compounds: Their Concentration and Behavior during in Vitro Gastrointestinal Digestion and Colonic Fermentation of Different Barley-Based Food Products Mosse, Juana Inés; Motilva, Maria José; Ludwig, Iziar A. Among cereals, barley (Hordeum vulgare L.) is notable for its high content of bioactives such as β-glucan and phenolic compounds, but it is not used as widely in human nutrition as wheat. To compare the impact of food formulation and processing on barley bioactives, crackers, cookies, and fresh pasta were prepared combining wheat and barley flour. After quantification of β-glucan and PCs in the barley flour and barley-based products, their behavior during in vitro gastrointestinal digestion and colonic fermentation was studied. The β-glucan and PCs were not drastically affected by processing. The amount of bioaccessible compounds after gastrointestinal digestion was lower than the amount retained in the undigested fraction. After in vitro colonic fermentation, β-glucan was mainly metabolized to acetic and propionic acids and PCs to phenylpropionic and phenylacetic acids. Based on the results of the study, the daily ingestion of barley-based foods may contribute to the intake of beneficial bioactive compounds.

Natural products to control biofilm on painted surfaces Bogdan, Sofia Tamara; Deya, Marta Cecilia; Micheloni, Oscar Bernardo; Bellotti, Natalia; Romagnoli, Roberto This paper aims to study five vegetables extracts as possible additives to control bacterial growth on indoor water-borne paints. The extracts were obtained from the weeds Raphanus sativus, Rapistrum rugosum, Sinapis arvensis, Nicotiana longiflora and Dipsacus fullonum, used in traditional medicine as antimicrobial compounds.As a general rule, results showed that tested paints were efficient in inhibitingbiofilm formation, specially that formulated with Nicotiana longiflora.The tested paints can be used to protect walls from microbial colonizationwhich shortened coatings useful life by discoloration and/or degradation.Concomitantly, indoor microbial colonization by aerosols could be alsodiminished. This is especially important in places that should have highstandards of environmental hygiene as in the food industry, health-care andsanitary centers, etc.

CO preferential oxidation on cordierite monoliths coated with CuO-CeO2/SBA-15 catalysts. Further insights into the physico-chemical aspects of the catalytic behavior Lacoste, Albano Mauricio; Tiscornia, Ines Silvia; Boix, Alicia Viviana CuO-CeO2/SBA-15 catalysts deposited over cordierite monoliths were prepared to be tested in CO preferential oxidation (COPrOx). The influence of molar ratio between the CuO and CeO2 active phases, their concentration, and the incorporation method into the mesoporous structure were analyzed. Powder catalysts were also studied in order to select the best formulations to coat the monolith walls. Four CuO/CeO2 molar ratios over SBA-15 were obtained by incipient wetness impregnation technique (successive impregnation and co-impregnation). The CuO/CeO2 = 0.55 ratio powder catalyst showed the best CO conversion in the temperature range studied, reaching 100% at 160 °C. The incorporation of active phases into the structured support produced similar or better catalytic behaviors. The addition of 10% CO2 slightly decreased the CO conversion, while the addition of 10% H2O partially deactivated the catalyst. The structured and powder catalysts prepared were characterized by N2 sorption, TEM, SEM, XRD, XPS and TPR in order to identify and relate their physico-chemical properties with the catalytic behavior.

Optimal design of dynamic experiments in the development of cybernetic models for bioreactors Luna, Martín Francisco; Martínez, Ernesto Carlos Cybernetic models of bioreactors are appealing due to their capacity to account for regulatory mechanisms in cell metabolism by modeling the synthesis of enzymes and their activities. For a given objective of interest, experimental data used to fit the cybernetic model parameters should be maximally informative. To excite purposefully the most relevant metabolic pathways, a dynamic experiment is designed by accounting for the sensitivity of the chosen objective to time-varying operating conditions. In this work, the bioreactor feeding profile and sampling times are designed to maximize the information content. A Bayesian optimization approach is proposed to solve the resulting mathematical program. As a case study, biomass production is used as the objective to be maximized in fed-batch cultivation of Saccharomyces cerevisiae growing on glucose as a carbon source. Experimental results demonstrate that the proposed approach helps to iteratively improve a cybernetic model by designing experiments that maximize the information content.

Effects of nutrient enrichment and grazing by an invasive filter feeder on phytoplankton biomass in a South West Atlantic coastal lagoon Bruschetti, Carlos Martin; Addino, Mariana del Sol; Luppi, Tomas Atilio; Iribarne, Oscar Osvaldo Invasive species can exert strong effects on structure and function of marine ecosystem. In estuaries, nutrient enhancement due to anthropogenic activities can increase microalgal biomass but invading filter-feeders can eventually regulate their abundance. This is what we hypothesized that is happening in a SW Atlantic coastal lagoon (Mar Chiquita, 37°40′S, 57°23′W, Argentina) invaded by the polychaete Ficopomatus enigmaticus. Here, we experimentally evaluated this hypothesis in a mesocosms experiment. Four treatments were performed: (T1) with nutrients (NO3, PO4 and NH4), (T2) with reefs and nutrients, (T3) with reefs, and (T4) without reefs or nutrients. Water samples were obtained to determine in vivo chlorophyll a (Chla) and nutrients concentration. Additionally, to evaluate the trophic position and particle selectivity by the polychaete, analysis of stable isotopes of particulate organic matter (POM) of the water and individuals were performed. Stable isotopes analysis showed that the main resource used by F. enigmaticus was the fraction of POM between 62 and 250 µm, showing particle selectivity and suggesting that the reefs have the potential to promote shifts in size, composition and biomass of local food source. Mesocosms experiments showed that nutrient supply increased the Chla concentration, but when reefs were added, the Chla decreased. Thus, nutrients increased the phytoplankton biomass but grazing by the reefs counteracted these increments. This indicates that an invader such as F. enigmaticus might modulate an anthropogenic impact via suspension feeding, and therefore its role in mitigate the consequences of eutrophication may be highly important.

Las reglas del discurso y su interpretación. Controversias en torno a las pragmáticas formales del lenguaje Paolicchi, Leandro Las pragmáticas formales del lenguaje (Apel y Habermas) han reconstruido un conjunto de presupuestos a los cuales les han atribuido un contenido ético específico y así los han convertido en el eje de una propuesta ética, la ética del discurso. Sin embargo, este contenido ético ha sido puesto en cuestión por varios autores. En este artículo, se abordan primero algunas de esas objeciones (II y III), luego se les da una respuesta tal como es posible reconstruirla en la obra de Apel y Habermas (IV) y finalmente se intenta mostrar un aspecto más interesante que esas objeciones plantean y como puede ésta ser desarrollada; The formal pragmatics of language (Apel and Habermas) have reconstructed a set of presuppositions to which they have attributed a specific ethical content and thus have made them the axis of an ethical proposal, the discourse ethics. However, this ethical content has been questioned by several authors. In this article, some of these objections are addressed first (II and III), then they are given an answer as it is possible to reconstruct it in the work of Apel and Habermas (IV) and finally try to show a more interesting aspect than those objections pose and how it can be developed.

Microbial endophytes that live within the seeds of two tomato hybrids cultivated in Argentina López, Silvina Marianela Yanil; Pastorino, Graciela Noemí; Franco, Mario Emilio Ernesto; Medina, Rocio; Lucentini, Cesar Gustavo; Saparrat, Mario Carlos Nazareno; Balatti, Pedro Alberto Tomato (Solanum lycopersicum L.) is probably the most important vegetable consumed around the world, and like other produce is affected by stresses and diseases that reduce the yield and production. The purpose of this work was to study the phytobiome of the tomato seeds of two hybrids in order to understand first of all whether tomato cultivars host similar groups of organisms, as well as their effect on the community structure, particularly of those microbes with the potential to promote growth and/or control plant pathogens. Different cultivars of tomato (genotypes) host significantly different endophytic communities, which is also reflected at the order level. These communities are particularly rich in spore-forming bacteria that have the ability either to promote plant growth or synthetize antimicrobial compounds that deter plant pathogens. We conclude that the seeds of the tomato cultivars Elpida and Silverio are sources of endophytic bacteria capable of synthetizing antifungal substances that could potentially be used for biocontrol against plant-pathogenic fungi.

Naturaleza y Derecho en "El Libro de la Selva" de Rudyard Kipling; Natureza e direito em “O livro da selva” de Rudyard Kipling Bailo, Gonzalo Luciano En este trabajo se analizan las nociones de Naturaleza y Derecho que gravitan en El libro de la selva de Rudyard Kipling. En función de distintas opiniones y eventos históricos, se explora la desestabilización de la frontera que separaba lo natural y lo social en la India colonial. Se trabaja también el contenido y alcance de la Rule of Law de los centros imperiales en relación a los sujetos ?no humanos? que habitaban los territorios coloniales. Se observa que, en El Libro de la selva, la línea que separa lo social y lo natural es una frontera en constante movimiento. Se concluye que, la desestabilización de los espacios y las subjetividades invita a una reflexión sobre el hospedaje de los extraños, de los seres no humanos y deshumanizados que habitan nuestras comunidades.; Neste artigo são analisadas as noções de natureza e de direito que gravitam em O livro da selva de Rudyard Kipling. Em função de distintas opiniões e eventos históricos, é explorada a desestabilização da fronteira que separava o natural e o social na Índia colonial. É trabalhado também o conteúdo e o alcance da Rule of Law dos centros imperiais em relação aos sujeitos “não-humanos” que habitavam os territórios coloniais. Observa-se que, em O livro da selva, a linha que separa o social e o natural é uma fronteira em constante movimento. Conclui-se que a desestabilização dos espaços e das subjetividades convida à reflexão sobre o alojamento dos estranhos, dos seres não-humanos e desumanizados que habitam nossas comunidades.; In this article the notions of nature and law that figure in Rudyard Kipling’s The jungle book are analyzed. Due to different opinions and historical events, the destabilization of the frontier that separated the natural and the social aspects in colonial India is explored. It also deals with the content and scope of the Rule of Law of the imperial centers in relation to the “nonhuman” subjects who inhabited the colonial territories. It is observed that, in The jungle book, the line that separates the social and the natural aspects is in constant movement. It is concluded that the destabilization of spaces and subjectivities invites reflection on the accommodation of strangers, nonhuman and dehumanized beings that inhabit our communities.

La regulación de las nanotecnologías en Argentina. Sobre polisemia e inestabilidad de los nano-objetos; Regulating Nanotechnologies in Argentina. About polysemy and instability of nano-objects; A regulação das nanotecnologias na Argentina. Sobre polissemia e instabilidade dos nano-objetos Bailo, Gonzalo Luciano Este trabajo se enmarca en una línea de los Estudios Sociales de la Ciencia y la Tecnología (ESCT) que estudia las relaciones entre derecho, democracia y nanotecnologías. Se analiza la experiencia de regulación de las nanotecnologías en Argentina en el período 2001-2017, a partir del rastreo y procesamiento de proyectos legislativos, iniciativas institucionales y discursos expertos comprometidos en la coproducción de un saber científico/jurídico “nano”. Se identificaron dos grandes etapas. En la primera etapa (2001-2006) se aborda el proceso que lleva a la creación por decreto de la Fundación Argentina de Nanotecnología (FAN) y se reseñan las críticas que recibió el modelo adoptado. En la segunda etapa (2007 en adelante), que inicia con la creación del Ministerio de Ciencia, Tecnología e Innovación Productiva (MINCyT), se estudia un modelo de gobierno “nano” que combina la institucionalidad experta y la estandarización normativa global. Se concluye que el carácter inestable y polisémico de los nano-objetos posiciona distintos tipos de conocimiento como mediadores de los formatos regulatorios e institucionales. En este sentido, el trabajo pretende contribuir: a) en general, a las discusiones actuales sobre los modos que tiene el derecho de estabilizar objetos controvertidos; b) en particular, a describir cómo los operadores legales visualizan a los nano-objetos en Argentina.; This paper is framed in the area of Science and Technology Studies (STS) that studies the relationships between law, democracy and nanotechnologies. It is analyzed nanotechnologies regulatory experience in Argentina between 2001-2017 by tracing and processing national bills, institutional initiatives and expert discourses committed on the coproduction of a “nano” scientific-legal knowledge. It isidentified two major phases. The first phase (2001- 2006) leadsto the creation by decree of the Argentinian Nanotechnology Foundation (FAN) and includesseveral critics about it. The second phase (2007-present) begins with the creation of Science, Technology and Productive Innovation Ministry (MINCyT) and adopts a “nano” governance model that combines expert institutionalism with the implementation of global standardization regulation. It is concluded that the instability and polysemic character of nanoobjectsrepositions varioustypes of knowledge as mediators ofregulatory and institutional models. This paper aims to contribute: a) in general, to current discussions about the legal modes ofstabilizing controversial objects; b) in particular, to describe how legal actors visualize nanoobjects in Argentina.; Este trabalho faz parte da linha de Estudos Sociais da Ciência e Tecnologia (ESCT) que estuda as relações entre direito, democracia e nanotecnologias. Analisa-se a experiência de regulação das nanotecnologias na Argentina no período 2001-2017, a partir do rastreamento e processamento de projetos legislativos, iniciativas institucionais e discursos de especialistas comprometidos na coprodução dum conhecimento científico/jurídico “nano”. Identificaram-se duas grandes etapas. A primeira (2001-2006) aborda o processo que leva à criação por decreto da Fundação Argentina de Nanotecnologia (FAN) e inclui as críticas ao modelo adotado. A segunda (2007- presente), que começa com a criação do Ministério da Ciência, Tecnologia e Inovação Produtiva (MINCyT), adota um modelo de governo “nano” que combina institucionalidade especializada e estandardização normativa global. Conclui-se que a natureza instável e polissêmica dos nano-objetos posiciona diferentes tipos de conhecimento como mediadores dos formatos regulatórios e institucionais. Nesse sentido, o trabalho visa contribuir: a) em geral, às discussões atuais sobre as formas em que o direito estabiliza objetos polêmicos; b) em particular, a descrever como os operadores legais visualizam aos nano-objetos na Argentina.

Comparing the asteroseismic properties of pulsating pre-extremely low mass white dwarf and δ Scuti stars Sánchez Arias, Julieta Paz; Romero, Alejandra Daniela; Corsico, Alejandro Hugo; Pelisoli, Ingrid; Antoci, Victoria; Kepler, S. O.; Althaus, Leandro Gabriel; Corti, Mariela Alejandra Context. Pulsating extremely low-mass pre-white dwarf stars (pre-ELMV), with masses between ∼0.15 M and ∼0.30 M, constitute a new class of variable stars showing g- and possibly p-mode pulsations with periods between 320 and 6000 s (frequencies between 14.4 and 270 c/d), driven by the κ mechanism operating in the second He ionization zone. On the other hand, main sequence δ Scuti stars, with masses between 1.2 and 2.5 M, pulsate in low-order g and p modes with periods in the range [700-28 800] s (frequencies in the range [3-123] c/d), driven by the κ mechanism operating in the He II ionization zone and the turbulent pressure acting in the HI ionization layer. Interestingly enough, the instability strips of pre-ELM white dwarf and δ Scuti stars nearly overlap in the Teff vs. log g diagram, leading to a degeneracy when spectroscopy is the only tool to classify the stars and pulsation periods only are considered. Aims. Pre-ELM white dwarf and δ Scuti stars are in very different stages of evolution and therefore their internal structure is very distinct. This is mirrored in their pulsational behavior, thus employing asteroseismology should allow us to distinguish between these groups of stars despite their similar atmospheric parameters. Methods. We have employed adiabatic and non-adiabatic pulsation spectra for models of pre-ELM white dwarfs and δ Scuti stars, and compare their pulsation periods, period spacings, and rates of period change. Results. Unsurprisingly, we found substantial differences in the period spacing of δ Scuti and pre-ELM white dwarf models. Even when the same period range is observed in both classes of pulsating stars, the modes have distinctive signature in the period spacing and period difference values. For instance, the mean period difference of p-modes of consecutive radial orders for δ Scuti model are at least four times longer than the mean period spacing for the pre-ELM white dwarf model in the period range [2000-4600] s (frequency range [18.78-43.6] c/d). In addition, the rate of period change is two orders of magnitudes larger for the pre-ELM white dwarfs compared to δ Scuti stars. In addition, we also report the discovery of a new variable star, SDSSJ075738.94+144827.50, located in the region of the Teff versus log g diagram where these two kind of stars coexist. Conclusions.The characteristic spacing between modes of consecutive radial orders (p as well as g modes) and the large differences found in the rates of period change for δ Scuti and pre-ELM white dwarf stars suggest that asteroseismology can be employed to discriminate between these two groups of variable stars. Furthermore, we found that SDSSJ075738.94+144827.50 exhibits a period difference between p modes characteristic of a δ Sct star, assuming consecutive radial order for the observed periods.

Adherencia a controles prenatales en el norte argentino desde la perspectiva de la interfaz social; Adherence to prenatal care in the north of Argentina from a social interface perspective Gonzalez Cowes, Valeria Laura; Landini, Fernando Pablo La adherencia a controles prenatales constituye una herramienta fundamental para disminuir la mortalidad materna. Usualmente se piensa la adherencia a partir de las características de las mujeres asistidas o de las particularidades del sistema de salud y sus agentes. Aquí se la aborda como emergente del modo en que se articulan las racionalidades y mundos de vida de profesionales y mujeres asistidas.Se realizó un estudio de casos múltiple buscando a comprender la adherencia a controles prenatales de mujeres rurales. Se realizaron 47 entrevistas en el norte argentino a mujeres embarazadas o que habían sido madres en los últimos años y a agentes del sistema de salud. El contenido de las entrevistas fue codificado con el apoyo del software Atlas Ti. El estudio permitió identificar dos tipos de interacción médico-paciente. El primero se caracteriza por una representación de las pacientes como ignorantes y desinteresadas, lo que lleva al agente a insistir con sus recomendaciones sin atender a las dificultades vividas por las mujeres. El segundo parte de concebir a las mujeres como cumplidoras y responsables, lo que permite un mayor diálogo, aún sin que se observe un interés claro por conocer el contexto de vida de las mujeres atendidas.; Adherence to prenatal care is fundamental for diminishing maternal mortality. Usually, adherence is considered in terms of assisted women’s characteristics or the specificities of the health systems and its professionals. Adherence is addressed here as the emergence of the articulation between the rationales and the life realities of physicians and pregnant women. Aiming to understand the adherence to prenatal care, a multiple case study was conducted in the North of Argentina. A total of 47 interviews of pregnant women or women that had been given birth in the last years and to agents of the health system were conducted. The content of the interviews was coded with the support of Atlas Ti software. The research identified two types or interaction between physicians and patients. The first one is based on physicians’ representation of assisted women as being ignorant and disinterested, which leads professionals to insist on their recommendations without taking into account problems faced by women. The second part of conceptions is supported in the representation of women as obedient and responsible, which enables a better dialogue between physicians and patients, albeit without the knowledge of a clear interest of the life context of the women being known.

Lipid bilayer-atomic force microscopy combined platform records simultaneous electrical and topological changes of the TRP channel Polycystin-2 (TRPP2) Lal S; Scarinci, María Noelia; Pérez, Paula Luciana; Cantiello, Horacio Fabio; Cantero, Maria del Rocio Ion channels are transmembrane proteins that mediate ion transport across biological membranes. Ion channel function is traditionally characterized by electrical parameters acquired with techniques such as patch-clamping and reconstitution in lipid bilayer membranes (BLM) that provide relevant information such as ionic conductance, selectivity, and gating properties. High resolution structural information of ion channels however, requires independent technologies, of which atomic force microscopy (AFM) is the only one that provides topological features of single functional channel proteins in their native environments. To date practically no data exist on direct correlations between electrical features and topological parameters from functional single channel complexes. Here, we report the design and construction of a BLM reconstitution microchamber that supports the simultaneous recording of electrical currents and AFM imaging from single channel complexes. As proof-of-principle, we tested the technique on polycystin-2 (PC2, TRPP2), a TRP channel family member from which we had previously elucidated its tetrameric topology by AFM imaging, and single channel currents by the BLM technique. The experimental setup provided direct structural-functional correlates from PC2 single channel complexes that disclosed novel topological changes between the closed and open sub-conductance states of the functional channel, namely, an inverse correlation between conductance and height of the channel. Unexpectedly, we also disclosed intrinsic PC2 mechanosensitivity in response to external forces. The platform provides a suitable means of accessing topological information to correlate with ion channel electrical parameters essential to understand the physiology of these transmembrane proteins.

Estimating Need, Demand and Supply in Primary Health Care Services: A Local Application in Argentina Elorza, Maria Eugenia; Moscoso, Nebel Silvana; Blanco, Anibal Manuel; Gentili, Jorge Osvaldo To provide equal access, health care provision should be distributed across geodemographic space based on need. In Argentina, the social security, publicly funded health care and private health care subsectors are responsible for delivering health services. In the public subsector, which is responsible for providing primary and secondary care mainly to the uninsured population, supply of services is not always associated with need. The lack of coordination between levels and subsectors makes it diffi cult to transform need into demand.