An early treatment with BKI-1748 exhibits full protection against abortion and congenital infection in sheep experimentally infected with Toxoplasma gondii Sánchez Sánchez, Roberto; Imhof, Dennis; Hecker, Yanina Paola; Ferre, Ignacio; Re, Michela; Moreno Gonzalo, Javier; Blanco Murcia, Javier; Mejías López, Elena; Hulverson, Matthew; Choi, Ryan; Arnold, Samuel; Ojo, Kayode; Barrett, Lynn; Hemphill, Andrew; Van Voorhis, Wesley; Ortega Mora, Luis Miguel Congenital toxoplasmosis in humans and in some mammalian species, such as small ruminants, is a well-known cause of abortion and foetal malformations. The calcium-dependent protein kinase 1 (CDPK1) inhibitor BKI-1748 has shown a promising safety profile for its use in humans and a good efficacy against Toxoplasma gondii infection in vitro and in mouse models. The rates of congenital infection and foetal damage in sheep seem to mimic the situation in human toxoplasmosis more closely than those in mouse models. Ten doses of BKI-1748 given every other day orally in sheep at 15 mg/kg exhibited therapeutic plasma levels for 23 days and no systemic or pregnancy-related toxicity was observed. In sheep experimentally infected at 90 days of pregnancy with a T. gondii oocyst dose which was lethal for all foetuses, the BKI-1748 treatment administered from 48 hours after infection led to complete protection against abortion and congenital infection. In addition, compared to infected/untreated sheep, treated sheep showed a drastically lower rectal temperature increase, higher IFNγ levels and none showed IgG seroconversion throughout the study. In conclusion, BKI-1748 treatment in pregnant sheep starting at 48 hours after infection was fully effective against congenital toxoplasmosis.

Pajaritos de papel: Análisis didáctico de una clase de formación vocal con aportes de la perspectiva semiótica; Paper birds: Didactic analysis or a vocal traning class with contributions from semiotic perspective Angelelli, Maria Belen; Martini, Ana Carolina; Carrera, Mariela El trabajo expone el análisis didáctico (Steiman, 2010) de una clase de Educación Vocal en la Universidad Provincial de Córdoba (UPC), Argentina. Desde una perspectiva transdisciplinaria y experiencial, identificamos núcleos problemáticos que giran en torno al espacio, el tiempo y los sujetos que intervienen en el proceso pedagógico de la clase, y en las decisiones y los supuestos que la docente llevó a cabo. Es decir, en aquellos componentes que hacen a las “configuraciones estéticas” de la clase (Roldán, 2022). En cuanto al aspecto metodológico, retomamos los registros de observación de la clase y los intercambios que tuvimos con la docente, también autora de este trabajo. Nos basamos en el guion conjetural que previamente había realizado y en su proyecto de cátedra. Desde las fronteras disciplinarias, entre la comunicación, la semiótica y la didáctica, planteamos nuestro análisis sobre el cronotopo áulico, un espacio-tiempo donde el celular es protagonista, aunque nuestros ojos no lo vean, atravesado por una cultura de la conectividad (Van Dijk, 2016).; This paper presents the didactic analysis (Steiman, 2010) of a Vocal Education class at the UPC. From a transdisciplinary perspective, located in the disciplinary and experiential boundaries in which we place ourselves as authors, we identify problematic nuclei that revolve around: space-time-subjects involved in the pedagogical process of the class, and in the decisions and assumptions that the teacher in charge carried out. That is, in those components that make up the "aesthetic configurations" of the class (Roldán, 2022). Methodologically, we took up again the observation records of the class and the exchanges we had with the teacher, also the author of this work, and we were guided by the conjectural script she had previously made and by her teaching project. From the disciplinary boundaries, between communication, semiotics and didactics, we propose our analysis of the classroom chronotope, a space-time where the cell phone is the protagonist although our eyes do not see it, also crossed by a culture of connectivity (Van Dijk, 2016).

“Turismo de trasplantes” e (in)securitización de las movilidades por salud; “Transplant Tourism” and (In)securitization of Health Mobilities Basualdo, María Lourdes El objetivo de este artículo es analizar el proceso de (in)securitización de las movilidades por salud definidas como “turismo de trasplante”. Específicamente indaga el surgimiento y circulación de la categoría “turismo de trasplantes”, así como ciertos usos políticos del concepto en regulaciones sobre trasplante y migración en Argentina. Sostiene que el “turismo de trasplantes” fue construido como un problema de (in)seguridad asociado a los peligros y amenazas que determinadas movilidades representan para el “orden nacional” de los trasplantes y para la “seguridad internacional” a partir de su asociación con modalidades ilícitas de extracción y comercialización de órganos. El artículo adopta una metodología cualitativa que articula análisis de diversas fuentes documentales y entrevistas con funcionarias/os de gobierno del contexto argentino.; This article seeks to analyze the process of in(in)securitization of health mobilities defined as "transplant tourism.” It focusses on the emergence and circulation of the "transplant tourism" category, as well as certain political uses of the concept in transplant and migration regulations in Argentina. It argues that "transplant tourism" was constructed as a problem of (in)security associated with the dangers and threats posed by certain types of mobilities to the "national order" of transplants and “international security,” because of its association with illicit forms of organ extraction and commercialization. The article uses a qualitative methodology combining the analysis of documentary sources with interviews with government officials in the Argentinean context.

Segmentación socioeconómica a usuarios finales de la energía eléctrica: Política tarifaria de Salta durante 2022; Socio-economic segmentation of electricity end-users: Salta's tariff policy during 2022 Hessling Herrera, Franco David Los servicios de energía son considerados básicos para hogares desde el punto de vista del derecho humano a la vivienda adecuada (art. 25 de la Declaración Universal de los Derechos Humanos). A principios de 2022, Argentina anunció un acuerdo de refinanciación de deuda con el FMI, en el que se establecía la quita de subsidios a esos servicios. A principios de abril, la Subsecretaría de Planeamiento Energético de la Nación emitió un informe técnico anticipando una “segmentación tarifaria”, y dos semanas después, Salta fue la primera jurisdicción en aplicar dicho criterio para la regulación de las facturas emitidas por la distribuidora provincial. En este artículo se caracteriza el Mercado Eléctrico Mayorista, se contextualiza la política tarifaria y se analiza específicamente la implementación de la segmentación en Salta. Para ello, se analizan documentos públicos y entrevistas realizadas para esta investigación con actores claves. Por último, se presentan algunas consideraciones sobre esa implementación y los efectos que podría tener en el mediano y largo plazo.; Energy services are considered basic for homes from the point of view of the human right to adequate housing (art. 25 of the Universal Declaration of Human Rights). At the beginning of 2022, Argentina announced a debt refinancing agreement with the IMF, which established the removal of subsidies for these services. At the beginning of April, the Subsecretary of Energy Planning of the Nation issued a technical report anticipating a "tariff segmentation", and two weeks later, Salta was the first jurisdiction to apply that criteria to regulate the invoices issued by the provincial distributor. This article characterizes the Wholesale Electricity Market, contextualizes the tariff policy and specifically analyzes the implementation of segmentation in Salta. To do this, public documents and interviews conducted for this research with key actors are analyzed. Finally, some considerations on this implementation and the effects it could have in the medium and long term are presented.

Determination of crystallographic texture in polycrystalline materials from wavelength-resolved neutron transmission experiments: application to high-symmetry crystals Vicente Alvarez, Miguel Angel; Malamud, Florencia; Santisteban, Javier Roberto Wavelength-resolved neutron transmission experiments are useful for characterizing the microstructure of macroscopic specimens with 2D spatial resolution perpendicular to the beam direction. The crystallographic texture can affect the neutron transmission in the thermal neutron energy range, which manifests as changes in the shape and height of Bragg edges as a function of neutron wavelength. Models have been proposed to predict the transmission of textured polycrystalline materials from knowledge of the material texture and have proved to accurately predict the observed transmission data. In recent work, a novel method was described and tested for obtaining texture integral parameters from the combined analysis of transmission data measured along several directions of a specimen in a hexagonal crystal Zr alloy. However, this procedure has limitations when dealing with high-symmetry crystal structures. In this work, a generalization of such a method based on the expansion of the orientation distribution function (ODF) in symmetric generalized spherical harmonics that is applicable to all crystal and sample symmetries is presented. Using this method, the low-order Fourier coefficients of the ODF can be estimated by analyzing transmission data obtained for a reduced set of beam directions. This method was verified using a cubic Cu sample, for which transmission data were available along five different directions. Two sample symmetries were assumed to reduce the number of Fourier coefficients of the ODF. In the case of cylindrical symmetry (fiber-type texture), the results were good; but in the case of orthorhombic symmetry, some bias was observed which was attributed to the reduced number of beam directions used to perform the evaluation.

Efecto de la intensidad de las lluvias sobre la acumulación de resina de Grindelia chiloensis en el suelo: implicancias para la restauración ecológica Rajnoch, María Gimena; Pérez, D. R.; Ravetta, Damián Andrés En ambientes áridos, debido a las presiones ambientales, las plantas han desarrollado una gran diversidad de metabolitos secundarios carbonados como las resinas, los politerpenos, las ceras, las gomas, entre otros. En particular, para las resinas se han encontrado varias funciones y se ha evidenciado la formación de costras fitoquímicas en el suelo por su acumulación bajo el canopeo de plantas como Grindelia chiloensis (Asteraceae) y Larrea divaricata (Zygophilaceae). Estas resinas modifican las propiedades físicas del suelo y la dinámica del agua en el suelo. En este trabajo caracterizamos el transporte de ácidos diterpénicos de G. chiloensis, generado por las lluvias, hacia la superficie del suelo y su acumulación. A partir de plántulas fue posible promover la formación de costras fitoquímicas en un período de dos años, en un experimento en el que se simularon precipitaciones de diferentes intensidades. Nuestros resultados también muestran que la intensidad de las precipitaciones determina la cantidad de resina removida y el contenido de resina del suelo. Debido a su efecto en las propiedades físicas del suelo y en la dinámica del agua, se propone que las costras fitoquímicas podrían modular procesos biológicos del suelo y tener una aplicación potencial para reiniciar la sucesión ecológica de sitios degradados o desertificados, al igual que las costras biológicas. En este contexto, los resultados obtenidos nos permiten pensar en diseños para la restauración que promuevan, imiten o aceleren el proceso natural de formación de costras.

El Genocidio como un dispositivo del olvido, la literatura como un dispositivo de la memoria: la Shoá y la Dictadura en la literatura argentina; Genocide as a tool to forget, literature as a tool to remember: the Shoah and the Dictatorship in Argentine literature Niemetz, Diego En la órbita de la postmemoria, heredar la experiencia de los mayores es un tópico revisitado una y otra vez por los escritores argentinos contemporáneos. Este trabajo se centra en el análisis de cuatro obras de la literatura argentina reciente que recuperan los relatos de los antepasados en forma de memoria familiar, pero que además proponen una reflexión acerca de la transmisibilidad de esas memorias y de la carga que supone. A partir de la superposición de experiencias familiares durante diferentes procesos genocidas, los textos postulan un borramiento de fronteras geográficas y temporales, que repercute en la presentación de los hechos y que, a partir de ese mecanismo, perduran en un presente sin solución de continuidad con el pasado ni con el futuro. Entre la persistencia y la superposición de las memorias genocidas y el trauma entendido como herencia, las producciones analizadas parecen perseguir un fin emancipatorio, de acuerdo a una de las famosas tesis sobre la historia de Walter Benjamin.; In the orbit of postmemory, inheriting the experience of the elders is a topic revisited over and over by contemporary Argentine writers. This article focuses on the analysis of four works of recent Argentine literature that recover the stories of ancestors in the form of family memories, but also propose a reflection on the transmissibility of these memories and the burden that they place on a person. Based on the overlapping of family experiences during different genocidal processes, the texts postulate an erasure of geographical and temporal borders, which affects the presentation of the facts and which, based on this mechanism, persist in a present that melts with the past or the future. Between the persistence and overlapping of genocidal memories and trauma understood as inheritance, the analyzed productions seem to pursue an emancipatory goal, according to one of Walter Benjamin’s famous theses on history.

Entre la opacidad y la transparencia: Cine, transición y monstruos; Between opacity and transparency: Cinema, transition and monsters Montes, Viviana Andrea Los estudios sobre el cine argentino de la primera década posdictadura se han planteado con frecuencia en términos de polarización: opacidad o transparencia, continuidad o ruptura con el cine previo. Otra clave de lectura interesante para abordar la producción audiovisual del decenio puede ser su eminente carácter transicional. El cine de esos años, por un lado, fue testigo y artífice de la transición política entre terrorismo de Estado y democracia; por otro, se vio conmovido por la transición tecnológica que impuso el avance del video como nuevo modo de producción y de circulación de imágenes. Al mismo tiempo vivenció su propia transición, que denominaremos transición cinematográfica.La transición se presenta como intersticio entre lo viejo y lo nuevo que se entrelazan creando formas que comparten características del tiempo pretérito y del porvenir sin identificarse completamente con ninguno de ellos. La coexistencia –no sin disputa- de narrativas y modos de representación es un rasgo tan frecuente como distintivo. Este trabajo busca indagar en los monstruos que habitan el claroscuro de ese cine constituyéndolo como espacio de querella entre lo viejo y lo nuevo. Esos monstruos se ligan a la acuciante presencia del pasado reciente en la narrativa cinematográfica del cine de la primera década de la posdictadura argentina y se expresan en las temáticas de los filmes, en la construcción de los espacios o en los vínculos interpersonales que componen los primeros trazos de la(s) memoria(s) que desde el campo cultural comenzaban a escribirse.; Studies on Argentine cinema in the first post-dictatorship decade have often been approached in terms of polarization: opacity or transparency, continuity or rupture with previous cinema. Another interesting reading key to approach the audiovisual production of the decade may be its eminent transitional character. The cinema of those years, on the one hand, was witness and architect of the political transition between State terrorism and democracy; on the other hand, it was moved by the technological transition that imposed the advance of video as a new mode of production and circulation of images. At the same time, it experienced its own transition, which we will call cinematographic transition. The transition is presented as an interstice between the old and the new that intertwine to create forms that share characteristics of the past and the future without being completely identified with either of them. The coexistence -not without dispute- of narratives and modes of representation is a feature as frequent as it is distinctive. This paper seeks to investigate the monsters that inhabit the chiaroscuro of this cinema, constituting it as a space of conflict between the old and the new. These monsters are linked to the pressing presence of the recent past in the cinematic narrative of the first decade of the Argentine post-dictatorship and are expressed in the themes of the films, in the construction of the spaces or in the interpersonal links that make up the first traces of the memories that were beginning to be written from the cultural field.

Una teoría del Estado para la democracia argentina Nosetto, Luciano Ezequiel Más de una vez sostuvo Eduardo Rinesi que la Argentina de los años 80 dedicó los mayores esfuerzos de su imaginación política a pensar la transición a la democracia, soslayando sin embargo la reflexión sobre el Estado. Si bien los monográficos de Guillermo O’Donnell sobre el Estado burocrático autoritario y de Oscar Oszlak sobre la formación del Estado argentino se producen hacia fines del 70 y comienzos del 80, lo cierto es que, a poco de andar la nueva década, el desafío de transitar a la democracia termina atrayendo la mayoría de los esfuerzos intelectuales de la época. De allí que Cecilia Lesgart pueda afirmar que “la transición a la democracia sustituyó las reflexiones sobre el Estado latinoamericano”. Esta marca de origen explicaría en parte el hecho de que, 40 años después, contemos con robustas y sopesadas teorías de la democracia, pero que nos cueste todavía articular una teoría del Estado consistente.

Multiple challenges in the development of commercial crops using CRISPR/Cas technology Ayub, Nicolas Daniel; Soto, Gabriela Cynthia The CRISPR/Cas system is a highly efficient and versatile tool for editing plant genomes, with the potential to accelerate breeding programs and improve the sustainability of food production. Nevertheless, technical limitations delay the rapid spread of the CRISPR/Cas system benefits in agriculture. The natural features of plant species, including reproductive behavior, ploidy levels, genetic diversity, and generation times, can significantly impact the introgression of edited traits into elite germplasms. The production and selection of edited events require the same level of effort as those of their transgenic equivalents. Additionally, edited alleles tend to be recessive or not fully dominant, which differs from dominant transgenic events. To accelerate the introgression of edited events into conventional and transgenic varieties, we suggest utilizing edits on single-copy genes that induce dominant mutations. In the absence of new, simple traits that provide exceptional economic benefits for large companies, like herbicide tolerance in transgenic crops, we propose the emergence of particular public grants for edited variety productions, especially when the introgression shows a high level of technical feasibility. In the context of climate change, these public actions must be taken quickly to alleviate significant reductions in crop production.

Robust Molecular Anodes for Electrocatalytic Water Oxidation Based on Electropolymerized Molecular Cu Complexes Amthor, Sebastian; Ranu, Koushik; Bellido, Carlos G.; Salomón, Fernando Federico; Piccioni, Alberto; Mazzaro, Raffaello; Boscherini, Federico; Pasquini, Luca; Gil Sepulcre, Marcos; Llobet, Antoni A multistep synthesis of a new tetra-amidate macrocyclic ligand functionalized with alkyl-thiophene moieties, 15,15-bis(6-(thiophen-3-yl)hexyl)-8,13-dihydro-5H-dibenzo[b,h][1,4,7,10]tetraazacyclotridecine-6,7,14,16(15H,17H)-tetraone, H4L, is reported. The reaction of the deprotonated ligand, L4−, and Cu(II) generates the complex [LCu]2−, that can be further oxidized to Cu(III) with iodine to generate [LCu]−. The H4L ligand and their Cu complexes have been thoroughly characterized by analytic and spectroscopic techniques (including X-ray Absorption Spectroscopy, XAS). Under oxidative conditions, the thiophene group of [LCu]2− complex polymerizes on the surface of graphitic electrodes (glassy carbon disks (GC), glassy carbon plates (GCp), carbon nanotubes (CNT), or graphite felts (GF)) generating highly stable thin films. With CNTs deposited on a GC by drop casting, hybrid molecular materials labeled as GC/CNT@p-[LCu]2− are obtained. The latter are characterized by electrochemical techniques that show their capacity to electrocatalytically oxidize water to dioxygen at neutral pH. These new molecular anodes achieve current densities in the range of 0.4 mA cm−2 at 1.30 V versus NHE with an onset overpotential at ≈250 mV. Bulk electrolysis experiments show an excellent stability achieving TONs in the range of 7600 during 24 h with no apparent loss of catalytic activity and maintaining the molecular catalyst integrity, as evidenced by electrochemical techniques and XAS spectroscopy.

Melanoma cells with acquired resistance to vemurafenib have decreased autophagic flux and display enhanced ability to transfer resistance Perez, Celia Noemí; Falcón, Cristian Roberto; Delgado Mons, Johinna Ileana; Cuello Orlandi, Federico; Sangiacomo, Mercedes; Fernandez Muñoz, Juan Manuel; Guerrero, Martín; Benito Rodriguez, Paula Guadalupe; Colombo, Maria Isabel; Zoppino, Felipe Carlos Martin; Alvarez, Sergio Eduardo Over the last years, the incidence of melanoma, the deadliest form of skin cancer, has risen significantly. Nearly half of the melanoma patients exhibit the BRAFV600E mutation. Although the use of BRAF and MEK inhibitors (BRAFi and MEKi) showed an impressive success rate in melanoma patients, durability of response remains an issue because tumor quickly becomes resistant. Here, we generated and characterized Lu1205 and A375 melanoma cells resistant to vemurafenib (BRAFi). Resistant cells (Lu1205R and A375R) exhibit higher IC50 (5–6 fold increase) and phospho-ERK levels and 2–3 times reduced apoptosis than their sensitive parents (Lu1205S and A375S). Moreover, resistant cells are 2–3 times bigger, display a more elongated morphology and have a modulation of migration capacity. Interestingly, pharmacological inhibition of sphingosine kinases, that prevents sphingosine-1-phosphate production, reduces migration of Lu1205R cells by 50 %. In addition, although Lu1205R cells showed increased basal levels of the autophagy markers LC3II and p62, they have decreased autophagosome degradation and autophagy flux. Remarkably, expression of Rab27A and Rab27B, which are involved in the release of extracellular vesicles are dramatically augmented in resistant cells (i.e. 5–7 fold increase). Indeed, conditioned media obtained from Lu1205R cells increased the resistance to vemurafenib of sensitive cells. Hence, these results support that resistance to vemurafenib modulates migration and the autophagic flux and may be transferred to nearby sensitive melanoma cells by factors that are released to the extracellular milieu by resistant cells.

A Stochastic Detection Models Comparison in Turbulent Flow Events Calandra, Maria Valeria; Marañon Di Leo, Julio The need to establish the downwind fluid dynamic field of aerodynamic bodies subjected to a givenvelocity field is well known, to verify their aerodynamic characteristics. In this context, differenttechniques allow us to establish the characteristics of the field. It is almost always necessary to carry out quantitative determinations to describe the field correctly, particularly when the field is made up of turbulent wakes. In this sense, in the experimental field, it is common to use hot-wire anemometry techniques, which have great capabilities to quantify high-frequency events. Previous work has analyzed the determination of changes in hot-wire anemometry signals for the detection of events in turbulent flows with different models, based on stochastic algorithms (CPM - Change Point Model).The present work aims to compare the results obtained previously with the application of different CPM models developed. Previously applied and evaluated measurements are used, the implementation of the models is carried out and the results are compared. All the algorithms used can detect changes in data that do not have a known distribution, i.e. non-parametric distributions, which are typical for turbulent flow field signals. Measurements of the fluctuating components of the wind tunnel velocity at a specific point are considering. The signals used correspond to periodic detachments downstream of a flow control device (Gurney mini-flap) at the trailing edge of an airfoil. The results show which are the best models to use for the experimental detection of such turbulent events in the flow field.

Aspectos ecológicos parasitarios en Pleurodema nebulosum (Anura: Leptodactylidae) en la región del monte, San Juan, Argentina; Parasitic ecological aspects in Pleurodema nebulosum (Anura: Leptodactylidae) in the monte region, San Juan, Argentina Castillo, Gabriel Natalio; González Rivas, Cynthia Jesica; Acosta, Juan Carlos Pleurodema nebulosum es una especie con amplia distribución en Argentina. Se analizó el parasitismo y su relación con el sexo, perturbación humana, masa y tamaño corporal. Se identificó al nemátodo Aplectana nebulosa (Cosmocercidae) en el intestino largo. Encontramos que la abundancia media parasitaria se asoció positivamente con el tamaño (longitud hocico-cloaca), sexo macho y perturbación humana. Un mayor tamaño corporal condujo a un aumento de la abundancia media de parásitos. Los machos de ambos ambientes estaban más parasitados que hembras. La abundancia media de parásitos fue mayor en el ambiente perturbado en comparación con el control. Discutimos nuestros resultados en un contexto ecológico. Se provee información novedosa sobre el parasitismo en esta especie de anfibio en el desierto del monte de Argentina.; Pleurodema nebulosum is a species with a wide distribution in Argentina. Parasitism and its relationship with host sex, human disturbance, and body mass were assessed. The nematode Aplectana nebulosa (Cosmocercidae) was identified in the large intestine. Mean parasitic abundance was found to be positively associated with size (snout-vent length), male sex and human disturbance. Larger body size led to an increase in the mean abundance of parasites. Males in both environments were more parasitized than females. The mean abundance of parasites was higher in the disturbed environment compared to the control. We discuss our results in an ecological context. Novel information is provided on parasitism in this amphibian species in the Monte Desert, Argentina.

Novel Fluoroquinolones with Possible Antibacterial Activity in Gram-Negative Resistant Pathogens: In Silico Drug Discovery Coba Males, Manuel Alejandro; Lavecchia, Martín José; Alcívar León, Christian David; Santamaría Aguirre, Javier Antibiotic resistance is a global threat to public health, and the search for new antibacterial therapies is a current research priority. The aim of this in silico study was to test nine new fluoroquinolones previously designed with potential leishmanicidal activity against Campylobacter jejuni, Escherichia coli, Neisseria gonorrhoeae, Pseudomonas aeruginosa, and Salmonella typhi, all of which are considered by the World Health Organization to resistant pathogens of global concern, through molecular docking and molecular dynamics (MD) simulations using wild-type (WT) and mutant-type (MT) DNA gyrases as biological targets. Our results showed that compound 9FQ had the best binding energy with the active site of E. coli in both molecular docking and molecular dynamics simulations. Compound 9FQ interacted with residues of quinolone resistance-determining region (QRDR) in GyrA and GyrB chains, which are important to enzyme activity and through which it could block DNA replication. In addition to compound 9FQ, compound 1FQ also showed a good affinity for DNA gyrase. Thus, these newly designed molecules could have antibacterial activity against Gram-negative microorganisms. These findings represent a promising starting point for further investigation through in vitro assays, which can validate the hypothesis and potentially facilitate the development of novel antibiotic drugs.

Cosmogenic nuclide moraine chronologies from Patagonia: A globally synchronic response of mountain glaciers during Termination 1? Soteres, Rodrigo L.; Sagredo, Esteban; Kaplan, M.R.; Martini, Mateo Antonio; Riquelme, Fabian; Schaefer, J.M. Surface-exposure dating of moraines reveals that Patagonian glaciers fluctuated at the pulsebeat mimicked in polar ice cores from both hemispheres. These findings favor hypotheses that invoke coupled oceanic–atmospheric drivers to generate and propagate millennial-scale climate shifts during Termination 1.

Estrogens receptors, nuclear coactivator 1 and ligand-dependent corepressor expression are altered early during induced ovarian follicular persistence in dairy cattle Notaro, Ulises Sebastián; Huber, Emilia; Stassi, Antonela Florencia; Ormaechea, Nadia Estefanía; Chiaraviglio, Juan Alberto; Baravalle, María Eugenia; Ortega, Hugo Hector; Rey, Florencia; Salvetti, Natalia Raquel Failure of ovulation can lead to follicular persistence, one of the main components of the pathogenesis of cystic ovarian disease (COD) in dairy cattle. Follicular persistence causes the permanence of a functional follicular structure in the ovary, which alters the cyclicity of the female and causes infertility. The aim of the present study was to evaluate the expression of estrogen receptors (ESR) 1 and 2, and the coregulatory proteins NCOA1, NRIP1 and LCOR by immunohistochemistry, in antral and preovulatory/persistent follicles in a model of follicular persistence induced by low levels of progesterone, to detect incipient changes during COD development, on the expected day of ovulation (P0) and after 5 (P5), 10 (P10) and 15 (P15) days of follicular persistence. Twenty-five Holstein cows were used, which were distributed in 5 groups: control group (n = 5), group P0 (n = 5), group P5 (n = 5), group P10 (n = 5), group P15 (n = 5). ESR1 expression was lower in antral follicles of the P5 (theca), P10 and P15 (theca and granulosa) groups relative to the control group (p < 0.05), and also lower in granulosa cells of persistent follicles of the P5, P10 and P15 groups than in dominant follicles of the control group (p < 0.05), without differences in theca cells. ESR2 expression showed no differences between groups. The ESR1:ESR2 balance favored ESR2 expression along the development of persistent follicles, as from 5 days of persistence (p < 0.05). NCOA1 expression was higher in granulosa cells of both antral and persistent follicles from the P0 group relative to the P5 and P10 groups, but showed no differences with the control and P15 groups (p < 0.05). Theca cells of antral and persistent follicles showed higher expression in the P0 and P15 groups in relation to the control, P5 and P10 groups (p < 0.05). No differences were detected for NRIP1 in antral, dominant and persistent follicles between groups. LCOR expression showed a decrease in granulosa cells of antral follicles from all persistence groups relative to the control group (p < 0.05). In theca cells, antral follicles of the P10 group showed lower LCOR expression than the control group (p < 0.05). LCOR expression was similar for dominant and persistent follicles. Considering that the ESR1:ESR2 balance favored ESR2 expression along the development of persistent follicles, as well as the decreased LCOR and NCOA1 expression, we may assume that, at the early stages of persistence, there is a negative regulation of ESR transcription. This coincides with the effects of estrogens through ESR on proliferation and apoptosis among other processes that favor follicular persistence. The results obtained provide relevant information in the knowledge of local events during the development of follicular persistence that could explain the failures in the reversion of the disease through hormonal treatments and the high recurrence rates reported for COD. In addition, it contributes to the study and identification of possible therapeutic targets, for the design of new treatments.

Harmonic almost complex structures on almost abelian Lie groups and solvmanifolds Andrada, Adrián Marcelo; Tolcachier, Alejandro An almost abelian Lie group is a solvable Lie group with a codimension one normal abelian subgroup. We characterize almost Hermitian structures on almost abelian Lie groups where the almost complex structure is harmonic with respect to the Hermitian metric. Also, we adapt the Gray–Hervella classification of almost Hermitian structures to the family of almost abelian Lie groups. We provide several examples of harmonic almost complex structures in different Gray–Hervella classes on some associated compact almost abelian solvmanifolds.

Desarrollo de herramientas cuantitativas para asistir en la toma de decisiones médicas relacionadas a discapacidades motrices Catalfamo Formento, Paola Andrea Lucia; Bonell, Claudia Edith; Aldonate, Julio Alberto; Barrera, Verónica; Cherniz, Analía Soledad; Dutto, Cecar; García Añino, Eloisa; Merino, Gabriela Alejandra; Muñoz Larrosa, Eugenia Soledad; Ravera, Emiliano Pablo; Riveras, Mauricio Se define como discapacidad motriz a la disminución total o parcial de la movilidad en uno o más miembros del cuerpo. Las personas realizan tratamientos de rehabilitación motriz para su reinserción activa a la sociedad. Para evaluar el resultado de los tratamientos se requieren de herramientas objetivas, confiables, reproducibles y tecno-lógicamente accesibles para las instituciones de salud de la región. El objetivo de este trabajo fue desarrollar herramientas cuantitativas de la ingeniería que cumplan con estos requisitos.Para ello se estudiaron herramientas de análisis del movimiento humano, tales como parámetros espacio-temporales, despeje mínimo del pie, índices de gasto energético y método de análisis de presiones plantares. Además, para facilitar la implementación de herramientas en los ámbitos clínicos, se desarrollaron softwares que permiten el cálculo automático de los parámetros. Finalmente, se diseñaron e implementaron protocolos de análisis del movimiento humano en centros regionales dedicados a la rehabilitación motriz.Los resultados de este proyecto muestran que es posible desarrollar herramientas basadas en la cuantificación de parámetros biomecánicos y fisiológicos, provistas desde los distintos campos de la ingeniería biomédica, que resultan objetivas, confiables, reproducibles y tecnológicamente accesibles para las instituciones de salud de la región.

Redes de conocimiento en relación a agendas endógenas como alternativa a la tensión internacional-local; Knowledge networks in relation to endogenous agendas as an alternative to international-local tension Oregioni, María Soledad; Avondet, Laura; Durán, María Sol La ciencia y la tecnología han ganado relevancia en las agendas de política pública en la medida en que se las reconoce como componentes imprescindibles de cualquier estrategia que pretenda hacer frente a «desafíos actuales», muchos de los cuales son definidos como tales por los países del Norte global. Posicionadas desde el Sur global, y bajo el entendimiento de la no neutralidad de los conocimientos, indagamos sobre dinámicas de producción de conocimiento y nos cuestionamos si las temáticas en agenda responden a problemáticas situadas. El presente trabajo muestra cómo, a partir de la identificación de una problemática concreta –la producción de cannabis medicinal– fue posible generar redes de cooperación sur-sur formales e informales, integradas por diferentes actores (territoriales e inter-nacionales) en las que se habilitaron nuevas dinámicas de producción de conocimiento a partir de la convergencia de diferentes tipos de saberes y actores. Específicamente, se analizó como caso de estudio la producción de conocimiento sobre cannabis medicinal en la Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN). Los resultados de la investigación permiten entender a la universidad como un actor fundamental en la producción de conocimiento y nos muestran cómo, a partir de una de sus funciones, la extensión, es posible crear nuevas dinámicas integrales de internacionalización en producción de conocimiento, destacando la importancia de las redes de cooperación sur-sur a la hora de dar respuesta a la tensión internacional-local en producción de conocimiento.; Science and technology have gained relevance in public policy agendas to the extent that they are recognized as essential components of any strategy that seeks to face «current challenges», many of which are defined as such by the countries of the global north. Positioned from the global South, and under the understanding of the non-neutrality of knowledge, we investigate the dynamics of knowledge production and question whether the topics on the agenda respond to situated problems. This paper shows how, based on the identification of a specific problem –the production of medical cannabis– it was possible to generate formal and informal South-South cooperation networks, made up of different actors (territorial and international) in which new dynamics of knowledge production from the convergence of different types of knowledge and actors. Specifically, the production of knowledge on medicinal cannabis at the National University of the Center of the Province of Buenos Aires (UNICEN) was analyzed as a case study. The results of the research allow us to understand the university as a fundamental actor in the production of knowledge; and they show us how from one of its functions, extension, it is possible to create new integral dynamics of internationalization in knowledge production, highlighting the importance of South-South cooperation networks when responding to tension international-local knowledge production.