Ciencia y Tecnología

Effect of temperature, light and storage on seed germination of Salvia plebeia R.Br., Leonurus japonicus Houtt., Mosla scabra (Thunb.) C.Y.Wu & H.W.Li and Perilla frutescens (L.) Britton

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Effect of temperature, light and storage on seed germination of Salvia plebeia R.Br., Leonurus japonicus Houtt., Mosla scabra (Thunb.) C.Y.Wu & H.W.Li and Perilla frutescens (L.) Britton Bhatt, Arvind; Carón, María Mercedes; Chen, Xingxing; Yu, Deshui; Niu, Yanli Understanding seed storage and germination ecophysiology is important for cultivation and for ex situ conservation of economically important species. Here we investigated the germination of four economically important plant species from the subtropical area of southeast China in relation to different environmental parameters. The seeds were subjected to different alternating temperatures (5/10 °C, 10/20 °C, 20/30 °C, 25/35 °C and 35/40 °C) and photoperiods (12 h light/12 h darkness and 24 h darkness). We also tested the effects of dry-storage on germination of selected species. The germination of all the species varied significantly with the temperature of incubation. The germination of L. japonicus and S. plebeia was highest under warm conditions, at 25/35 °C and 20/30 °C, respectively. However, seeds of M. scabra and P. frutescens showed the highest germination at 10/20 °C and the lowest at 35/40 °C, respectively. Exposure to 12 h of light enhanced the germination in all species (germination reached between 19 % and 87 %). Seed germination percentages of all the species were significantly enhanced by storage time, although the extent was species-specific. This study established a successful approach for optimizing seed germination of different species of Lamiaceae that could be utilize for their large scale production.

Contra la verdad de sí: infamias, prácticas sexuales y formas de resistencia queer en el siglo XIX

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Contra la verdad de sí: infamias, prácticas sexuales y formas de resistencia queer en el siglo XIX; Against the Truth of Oneself: Infamy, Sexual Practices, and Forms of Queer Resistance in the 19th Century Gutierrez, Maria Laura El artículo debate en torno a la mentira y la verdad a través del análisis de la utilización política de ambas nociones como parte de una estrategia de defensa y afirmación de experiencias queer en el siglo XIX inglés. Mediante el abordaje de dos juicios emblemáticos para la historia de la sexualidad lésbica y gay –los de Woods and Pirie y el de Oscar Wilde– este texto busca rescatar cómo la imaginación, la pose y el artificio se erigieron dentro del mundo queer como formas de existencia contra la injuria y la creación imperialista de una naturaleza heterosexual, blanca y reproductiva. No encontraremos aquí estrategias puras de resistencia, limpias de clasismo o de racismo, sino modulaciones que usan los artilugios de la obligación a la verdad de sí y de los discursos dominantes para actualizar la incomodidad en lo que espera ser visto –incluso cuando se reproduzca– y subsistir en las opacidades.; This article enters the debate around lies and truth through the analysis of the political utilization of both notions as part of a strategy of defense and affirmation of queer experiences in nineteenth-century England. By means of examining two emblematic cases in the history of sexuality and of the lesbian/gay communities –that of Woods and Miss Pirie and Oscar Wilde's– this text seeks to underscore how imagination, pose and artifice against nature erected themselves within the queer world as forms of existence against injury and the imperialist creation of a heterosexual, white, and reproductive nature. We will not find here pure strategies of resistence, devoid of classism or racism, but modulations that resort to gadgets of the obligation of the truth of oneself and of the dominant discourses to actualize the uneasiness in what is expected to be seen – even when it is reproduced– and to subsist in opacities.

Conocimiento sobre rieles: el papel del ferrocarril en las prácticas de extensionismo y experimentación agropecuaria en regiones argentinas (ca. 1908-1930)

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Conocimiento sobre rieles: el papel del ferrocarril en las prácticas de extensionismo y experimentación agropecuaria en regiones argentinas (ca. 1908-1930); Knowledge on track: the role of railroad in farming extension services and experimentation in argentine regions (ca. 1908-1930) Martocci, Federico Carmelo; Moyano, Ricardo Daniel; Rodriguez Vázquez, Florencia La divulgación de conocimientos agropecuarios con base técnica fue una de las tantas prioridades del Ministerio de Agricultura de la Nación, que cristalizó en la organización de Agronomías Regionales para abastecer las demandas de diferentes regiones del país. En particular, la implementación de un sistema de Enseñanza Extensiva demandó la asociación estratégica con firmas ferroviarias para ampliar la cobertura de aquel servicio, en términos de extensión y de potenciales destinatarios. Es objetivo de este artículo conocer las primeras discusiones sobre el sistema de las Agronomías Regionales y su grado de implementación a partir del decisivo apoyo de las firmas ferroviarias, en los casos tucumano, mendocino y pampeano.; The dissemination of technically based farming knowledge was one priority of the National Agricultural Ministry, solidified through the creation of Regional Agronomy Offices to meet the different regional demands across the country. Particularly, implementing an outreach system required the strategical partnership with the railroad companies to broaden the scope of the service, both in terms of extension and potential recipients. This article is intended to explore the first discussions on the Regional Agronomy network and the scale of implementation reached thanks to the railroad's support, in the cases of Tucumán, Mendoza, and La Pampa.

Althusser desde América Latina: confluencias con José Aricó, René Zavaleta y Agustín Cueva

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Althusser desde América Latina: confluencias con José Aricó, René Zavaleta y Agustín Cueva Tzeiman, Andrés En este trabajo nos interesa recuperar algunas reflexiones llevadas a cabo en nuestra región que pueden ser puestas en diálogo con ciertas elaboraciones desplegadas por Althusser, mostrando un aire de familia entre la producción teórica de estos autores latinoamericanos y la del filósofo marxista francés. Del mismo modo, nos importa poder mostrar cómo algunos desarrollos en un campo problemático común pueden dar cuenta de matices respecto a determinados aspectos teóricos. Siguiendo esa línea de razonamiento, en estas páginas nos proponemos establecer una conversación entre dos trabajos en particular de Althusser y tres figuras destacadas de la teoría política y social latinoamericana. Al respecto, debemos hacer dos aclaraciones. La primera de ellas es que nos referiremos aquí a dos trabajos del filósofo francés que fueron publicados de forma póstuma (hablamos de Sobre la reproducción y ¿Qué hacer?), de manera tal que nuestro propósito no será el de hallar un intercambio documentado, sino el de indagar en nudos comunes de reflexión, es decir, en aportes fructíferos al campo de la teoría marxista que pueden haber llevado el sello de un cruce productivo entre la obra de Althusser y los abordajes realizados desde América Latina. El segundo señalamiento indica que nos focalizaremos en algunas contribuciones puntuales de tres intelectuales latinoamericanos: José Aricó, René Zavaleta y Agustín Cueva. Pues consideramos que en ciertos escritos de cada uno de ellos es posible establecer una intersección con determinadas zonas de indagación teórica propuestas por Althusser.

Orden y desarrollo: Claves para un abordaje de la trayectoria político-intelectual de Mariano Grondona

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Orden y desarrollo: Claves para un abordaje de la trayectoria político-intelectual de Mariano Grondona Vicente, Martin Alejandro La heterogénea trayectoria político-intelectual de Mariano Grondona, abogado, periodista, ensayista, docente, consultor, tiene en el par decimonónico orden y desarrollo una clave para su abordaje. Generacionalmente convertida en orden y desarrollo, esa dinámica marcó un perfil que recorrió siete décadas de vida política e intelectual de la Argentina, configurando tres grandes etapas, que este artículo recorre: en la primera, Grondona apostó por el desarrollo tanto en democracia como por vías autoritarias, entre 1955 y 1983; agotado el ciclo de alternancia cívico-militar, lo hizo por una democracia liberal amplia y plural, capaz de crear una cultura política para el desarrollo con ejes de centro-izquierda y centro-derecha, de 1983 a 2001; finalmente, tras la crisis del 2001 buscó enfatizar el contenido republicano de la democracia como orden capaz de albergar el desarrollo.

La expansión urbana de la ciudad de Tandil 1991-2020 mediante el uso del Landscape Expansion Index (LEI)

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La expansión urbana de la ciudad de Tandil 1991-2020 mediante el uso del Landscape Expansion Index (LEI); The urban expansion of the city of Tandil 1991-2020 through the use of the urban expansion index (LEI) Linares, Santiago; Iturrioz, Ignacio; Di Nucci, Josefina Inés Los índices del paisaje se han usado comúnmente para cuantificar las formas, magnitudes y patrones de distribución espacial. Existen diversos índices, dentro de los cuales la dinámica del crecimiento urbano es una de ellas. Cuantificar los patrones paisajísticos y analizar sus cambios es esencial para el monitoreo y la evaluación de las consecuencias ecológicas de la urbanización derivadas de los procesos de fragmentación urbana. En este trabajo, se aplica un nuevo índice de expansión urbana (LEI, Landscape Expansion Index), que permite capturar la información acontecida de los procesos espacio-temporales de formación de un patrón de expansión e identifica tres tipos de crecimiento urbano: “de relleno”, “expansión de borde” y “periférico”. Se extrajeron las superficies edificadas y se aplicó LEI sobre ellas en la ciudad de Tandil para los períodos 1991-2001, 2001-2010 y 2010-2020, constatando que el tipo de crecimiento “periférico” es el predominante en los últimos años.; Landscape indexes have been commonly used to quantify spatial distribution shapes and patterns of ground covers. Different types of indexes exist according to various application areas, being the urban growth dynamics one of them. Quantifying landscape patterns and analyzing their changes is essential to evaluate the urbanization ecological consequences of urban fragmentation and dispersion processes. In this article, it is applied a new Landscape Expansion Index (LEI), that unlike conventional indexes, allows to capture the space-temporal information of the urban expansion process through patterns. This tool identifies three types of urban growth: “infilling”, “edge expansion” and “outlying”. Built surfaces were extracted and LEI was applied on them to analyze the urban expansion dynamic in the city of Tandil in the periods of 1991-2001, 2001-2010 and 2010-2020. Verifying that the predominant urban growth type in the last years is the “outlying”.

Toda Pamela es política: Intensidades románticas, tradiciones sociosexuales y series televisivas

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Toda Pamela es política: Intensidades románticas, tradiciones sociosexuales y series televisivas; Every Pamela is political: Romantic intensities, socioexual traditions and television series Gómez Ponce, Fernando Ariel La exitosa serie Pam & Tommy (Hulu, 2022) promete una reivindicación feminista de Pamela Anderson: estereotipo que obsesionó al capitalismo heterosexual en los 90. Sin embargo, esa aparente mirada crítica puede cuestionarse, en tanto la narrativa no parece abandonar una idea de amor bastante tradicional. En torno a las propuestas de Eva Illouz y Fredric Jameson, la categoría de intensidades románticas colaborará con la interpretación de esa encrucijada, a la vez que arroja luz sobre nuestra idea cultural de la experiencia amorosa: afectividad que, aún incorporando las mutaciones de la posmodernidad, se aferra a los grandes mitos del amor-pasión y a sus instituciones. Esa intensidad se constatará en uno de los lugares comunes que Pamela Anderson y Tommy Lee sintetizan: la pareja rockstar, aquella que demuestra ser conservadora por su resguardo en la familia burguesa y reproductora y en los roles canónicos de la masculinidad protectora y la feminidad progenitora. El objetivo de este artículo es así corroborar cómo en nuestra cultura masiva el amor romántico todavía se mantiene como una de las utopías favoritas, si bien contradictorias, del capitalismo.; The successful series Pam & Tommy (Hulu, 2022) promises a feminist vindication of Pamela Anderson: a stereotype that obsessed heterosexual capitalism in the 90s. However, that apparent critical view can be questioned, as the narrative does not seem to abandon a traditional idea of love. Around the proposals of Eva Illouz and Fredric Jameson, the category of romantic intensities will collaborate with the interpretation of this crossroads, shedding light on our cultural idea of the love experience: affectivity that, even incorporating the mutations of postmodernity, clings to the great myths of love-passion and its institutions. That intensity will be verified in one of the common places that Pamela Anderson and Tommy Lee synthesize: the rockstar couple, which proves to be conservative due to its protection in the bourgeois and reproductive family and in the canonical roles of protective masculinity and progenitor femininity. The objective of this article is thus to corroborate how in our mass culture romantic love is still maintained as one of the favorite and contradictory utopías of capitalism.

TLR9 activation is required for cytotoxic response elicited by baculovirus capsid display

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TLR9 activation is required for cytotoxic response elicited by baculovirus capsid display Molinari, Maria Paula; Crespo, Maria Ines; Molina, Guido Nicolás; Dho, Nicolás Daniel; Marinho, Fábio A. V.; Maletto, Belkys Angélica; Leclerc, Claude; Oliveira, Sergio C.; Taboga, Oscar Alberto; Cebrián, José Ignacio; Morón, Sergio Gabriel Although the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) infects lepidopteran invertebrates as natural hosts, represents an efficient vector for vaccine development. Baculovirus surface display induces strong humoral responses against viruses and parasites. A novel strategy based on capsid display carrying foreign antigens in the AcMNPV particle further improved the immune response by eliciting CD8+ T cell activation. In this study, we analyze the intracellular mechanisms and signalling pathways involved in CD8+ T cell activation by capsid display. Our results show that baculovirus can attach to the cell surface, enter dendritic cells (DCs), transit within endocytic vesicles and escape to the cytosol for further degradation by the proteasome. We found that the availability of viral proteins, endosomal acidification, and proteasome activity are needed for efficient Major Histocompatibility Complex class-I presentation by baculovirus carrying Ovalbumin in the viral capsid. Importantly, we demonstrated with this strategy that the induction of cytotoxic T cells and IL-12 production by DCs are TLR9-dependent and STING-independent. Finally, our study shows differential intracellular processing for capsid and surface baculovirus proteins in DCs and highlights the role of different danger receptors during cytotoxic T cell priming through the capsid display delivery system, which could lead to improved baculovirus-based vaccines development.

Development of active biodegradable films based on chitosan and essential oil to prevent infestation of Plodia interpunctella (Lepidoptera: Pyralidae)

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Development of active biodegradable films based on chitosan and essential oil to prevent infestation of Plodia interpunctella (Lepidoptera: Pyralidae) Jesser, Emiliano Nicolás; Castillo, Luciana Andrea; Alonso, Yanela Natalin; Urrutia, Rodrigo Iñaki; Murray, Ana Paula; Domini, Claudia Elizabeth; Werdin Gonzalez, Jorge Omar Plodia interpunctella Hübner (Lepidoptera: Pyralidae) is probably the most common moth pest of stored products. Its larvae are considered very effective packaging penetrators, causing serious losses. Therefore, the development of new food packaging technologies to prevent larvae infestation is crucial. In the present work, geranium and peppermint essential oils (EO) were used to elaborate chitosan (Qx) based films (Qx-EO films) at different concentrations (0% (control), 0.1%, 0.25% and 0.5% w/w). The Qx-EO films were tested in repellent and penetration assays for 72 h and 15 days, respectively. In both bioassays, most effective Qx-EO films were those containing geranium and peppermint EO at 0.5% w/w. In repellent assays, Qx-geranium EO films were more effective than Qx-peppermint EO films. In penetration assays, both films reduced penetration percentage to 10% (penetration percentage for control ≈ 50%). Then, possible structural changes, thermal stability and mechanical properties of Qx films containing EOs at 0.5% w/w were analyzed. The loading efficiency of Qx-geranium EO and peppermint EO films were close to 90% and 35%, respectively. It was demonstrated that EO incorporation allows conserving mechanical properties of control films. It is important to emphasize that these films showed repellent activity and could prevent contamination with P. interpunctella larvae.

Screen use, sleep duration, daytime somnolence, and academic failure in school-aged adolescents

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Screen use, sleep duration, daytime somnolence, and academic failure in school-aged adolescents We surveyed 1,257 12- to 18-year-old adolescents attending 52 schools in urban or suburban areas of Argentina. We recorded the daily exposure to various screen-based activities, including video- and online-gaming, social media, TV or streaming. Screen time and device type in the hour before bedtime, sleep patterns during weekdays and weekends, somnolence (Pediatric Daytime Sleepiness Scale score), and grades in language and mathematics were also assessed.

Datos acerca del cálculo de la erosión hídrica y su relación con el avance agrícola en el partido de Benito Juárez (Buenos Aires, Argentina), para las campañas 1989/90, 2002/03 y 2014/15

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Datos acerca del cálculo de la erosión hídrica y su relación con el avance agrícola en el partido de Benito Juárez (Buenos Aires, Argentina), para las campañas 1989/90, 2002/03 y 2014/15 Información perteneciente al desarrollo del artículo "EROSIÓN HÍDRICA Y SU RELACIÓN CON EL AVANCE DE LA AGRICULTURA EN EL SUDESTE BONAERENSE, ARGENTINA. EL CASO DEL PARTIDO DE BENITO JUÁREZ DURANTE LAS CAMPAÑAS 1989/90, 2002/03 Y 2014/15". Se adjunta la ubicación del área de estudio, figura con la superposición entre las Unidades Ecológicas (UEc) que conforman el partido y clasificaciones supervisadas de los años 1990, 2003 y 2015; y planilla de datos respecto de superficie agrícola por UEc, información de los distintos factores para el cálculo de las tasas de erosión y pérdida de suelos y cálculo de los niveles de tolerancia de los suelos a la erosión.

Datos acerca de la extracción de nitrógeno y fósforo en el partido de Benito Juárez (Buenos Aires, Argentina) y su relación con el proceso de agriculturización

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Datos acerca de la extracción de nitrógeno y fósforo en el partido de Benito Juárez (Buenos Aires, Argentina) y su relación con el proceso de agriculturización Información perteneciente al desarrollo del artículo "EFECTOS DE LA AGRICULTURIZACIÓN SOBRE LA EXTRACCIÓN DE NUTRIENTES EN LA REGIÓN PAMPEANA ARGENTINA. EL CASO DEL N Y P EN EL PARTIDO DE BENITO JUÁREZ". Se adjunta la ubicación del área de estudio, figuras con producción y rendimientos de los principales cultivos y % de tierras fertilizadas realmente, documento con balances de N y P por Unidad Ecológica (UEc) del partido, documento con cálculo de los balances generales y planillas con información y cálculos asociados a dosis de aplicación y relación entre balances y superficie agrícola.

Arturo A. Roig: a cien años de su nacimiento

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Arturo A. Roig: a cien años de su nacimiento Contardi, Laura Aldana; Olalla, Marcos Javier Arturo Andrés Roig nació el 16 de julio de 1922 y falleció el 30 de abril de 2012 en Mendoza (Argentina). Cursó estudios de Filosofía en la Universidad Nacional de Cuyo y egresó de la misma en 1949. Obtuvo en 1953 una beca para realizar en Francia estudios sobre filosofía antigua con el profesor Pierre-Maxime Schuhl, estancia de estudios que duró dos años. A su regreso, su labor se concentró en la profundización de los estudios platónicos, tarea que abarcaría más de diez años. Se abocó a la reconstrucción de la historia cultural de su provincia natal y a la investigación de la presencia del krausismo y del espiritualismo en el Río de la Plata. Fue docente de la Facultad de Filosofía y Letras desde 1959 hasta 1975, momento en que se vio obligado, junto a su familia, a exiliarse y dejar la cátedra por la persecución política ocurrida durante el gobierno de María Estela de Perón y la gestión del ministro de Educación Oscar Ivanissevich. Entre 1973 y 1974 ocupó el cargo de secretario académico de la Universidad Nacional de Cuyo, gestión en la que impulsó transformaciones en la organización de los saberes en la universidad y particularmente en la Facultad de Filosofía y Letras, en el marco de una pedagogía participativa alentada por ideales democráticos. En su exilio residió un breve tiempo en México y luego en el Ecuador, país en el que vivió hasta su regreso a la Argentina en 1984. Durante esos años dedicó sus estudios al rescate de la historia del pensamiento social y filosófico del Ecuador, fundó y dirigió el Instituto de Estudios Latinoamericanos de la Pontificia Universidad Católica del Ecuador. El gobierno ecuatoriano le otorgó la condecoración al mérito cultural en 1983. Ya en Mendoza fue restituido por orden judicial en su cargo universitario, del que se jubiló en 1985, e incorporado en el Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) como Investigador Principal y como Director general del Centro Regional de Investigaciones Científicas y Técnicas de Mendoza (CRICYT), dependiente del CONICET. Unos años después fue director fundador del Instituto de Ciencias Humanas, Sociales y Ambientales (INCIHUSA).

Prediabetes is more than a pre-disease: additional evidences supporting the importance of its early diagnosis and appropriate treatment

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Prediabetes is more than a pre-disease: additional evidences supporting the importance of its early diagnosis and appropriate treatment Elgart, Jorge Federico; Torrieri, Rocio; Ré, Matias Alberto; Salazar, Martin; Espeche, Walter; Angelini, Julieta Maria; Martinez, Camilo; Martinez, Jorge Guillermo; Giampieri, Cecilia; Etchegoyen, Graciela Susana; Ricart, Juan Pablo Antonio; Rodriguez, María Elvira; Gagliardino, Juan Jose Aim: To identify Prediabetes (PreD) as early and serious diabetes step using clinical-biochemical characteristics in the population of the Primary Prevention Diabetes Buenos Aires (PPDBA) study. Methods: PPDBA Study evaluated benefits of adopting healthy lifestyles to prevent T2D. It recruited people 45–75 years of age with PreD (impaired fasting glycaemia [IFG], impaired glucose tolerance [IGT] or both, American Diabetes Association criteria), using an opportunistic approach. They completed a FINDRISC questionnaire, and those with a score ≥13 points were invited to participate. When they accepted, we performed an oral glucose tolerance test (OGTT) with a complete lipid profile and HbA1c while physicians completed a clinical history. We recruited 367 persons, and depending on OGTT results, the sample was divided into normals (NGT), PreD, or with diabetes (last one was excluded in our analysis). Data were statistically analyzed using parametric and nonparametric tests and logistic regression to identify parameters associated with PreD. Results: From the recruited (n = 367) 47.7% have NGT, 48.5% PreD and 3.8% unknown T2D (excluded). People with PreD were significantly older, with a higher percentage of overweight/obesity, BMI, and larger waist circumference than NGT. They also showed significantly higher fasting and 2 h post glucose load, HbA1c, and triglyceride levels. No significant differences were recorded in the blood pressure, lipid profile though both groups had abnormally high LDL-c values. They also had a larger percentage of TG/HDL-c ratios (insulin resistance indicator) (55% vs. 37.5%). Logistic regression analysis showed that PreD was significant associated with age, waist circumference, and triglyceride above target values. Conclusion: Our findings showed that clinical and biochemical parameters were significantly different between people with PreD and those with NGT. This evidence supports the concept that PreD is a serious dysfunction, which should be early diagnosed and treated properly to prevent its transition to T2D and its complications.

Corrosion protection properties of polypyrrole coatings formed onto 316 L SS from a solution containing molybdate and salicylate

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Corrosion protection properties of polypyrrole coatings formed onto 316 L SS from a solution containing molybdate and salicylate Ramos, Camila; González, María Belén; Brugnoni, Lorena Inés; Saidman, Silvana Beatriz The electrosynthesis of PPy films onto 316L stainless steel fromnearly neutral and alkaline solutions containing molybdate and salicylate isreported. The corrosion behaviour of the coatings constituted by hollow rectangularmicrotubes was monitored in Ringer solution using open circuit potentialmeasurements, polarization techniques and electrochemical impedance spectroscopy.The polymer formed in a solution of pH 8 was the most efficient interms of corrosion protection. The coating significantly reduced the pitting corrosionof the substrate. The results were interpreted taking into considerationthe polymer electroactivity, the galvanic interaction polypyrrole/substrate andthe nature of dopants. Silver species were effectively immobilized onto the hollowrectangular-sectioned microtubes of PPy. The modified coatings demonstratedthat they have a good bactericidal activity against Escherichia coli andthey were also able to protect the substrate against corrosion in Ringer solution.

Molecular detection and parasite load of Trypanosoma cruzi in digestive tract tissue of Chagas disease patients affected by megacolon

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Molecular detection and parasite load of Trypanosoma cruzi in digestive tract tissue of Chagas disease patients affected by megacolon Pinto, Lilian; Schijman, Alejandro Gabriel; Alonso Padilla, Julio; Lozano, Daniel; Cruz Torrico, Mary; Gamba, Pietro; Torrez, Margarita; Lozada, Vania; Cartagena, Karina; Sanchez, Jareth; Torrico, Faustino; Gascon, Joaquim Chagas disease, caused by the Trypanosoma cruzi parasite in the Americas affects ∼ 7 million people, 30% with cardiac tissue damage and 10-15% with digestive disorders. In this study, we have developed a protocol to detect the presence of the parasite and estimate its load in resected dysfunctional tissue segments of chronically infected patients with digestive megacolon. We have included samples from 43 individuals, 38/5 with positive/negative serology for Chagas disease and digestive syndromes. Samples of 1.5 to 2.0 cm2 were taken from different points of the dysfunctional digestive tract in specialized centres in Cochabamba, Bolivia. T. cruzi cultures were performed by inoculation with NNN-LIT culture medium, and genomic material was obtained from the samples for multiplex qPCR with TaqMan probes targeting satellite nuclear DNA. Cultures failed to isolate T. cruzi but qPCR reached a sensitivity of 42.1% (16/38) with all three spots and in triplicate. A new quantification methodology using synthetic satellite DNA as quantitation standard revealed parasite loads ranging from 2.2 × 102 to 1.0 × 106 satellite DNA copies/μl. Positive samples from the distal end showed a higher parasite load. The results of the present study strengthen and add further evidence to previous findings in an experimental mouse model of chronic T. cruzi infection, providing a valuable tool to improve scientific knowledge on the relevance of the digestive tract in parasite persistence, and underline the need of a better understanding of host-pathogen interaction in digestive tissues, considering pathophysiology, disease immunology and response to treatment.

Between Hegel and Hawking - Kierkegaard: Contribution to Epistemology

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Between Hegel and Hawking - Kierkegaard: Contribution to Epistemology Binetti, María José; Máhrik, Tibor; Kobylarek, Aleksander; Zimny, Jan; Vladilenovna Telezhko, Irina Background: This presented study focuses on the issue of the possibilities and limits of humanrationality in the theory of knowledge from the perspective of Søren Kierkegaard. His opposition to the Hegelian rationalism of his time anticipates similar opposition to the strict naturalismof this time represented by Stephen Hawking. Kierkegaard‘s perspective of understanding thebasic categories of human existence form an intricate texture consisting of more central andmore peripheral beliefs, mutually supporting each other, while referring to such concepts in theirfundamental philosophical positions.In the study, we present Kierkegaard‘s relevant starting points and arguments with which headdresses the problem of a hyper-rationalistic approach to life, regardless of whether it is a metaphysical ambition or an existential understanding of life.Conclusion: We argue here that Kierkegaard‘s concept of existential passion, directed in thehope of an infinite future, provides not only the necessary framework for finding answers to contemporary epistemological challenges, especially in the field of the humanities, but also a goodprotection against the trap of idealism and strict naturalism.

Literaturas y derechas: Historizar los conceptos, ampliar los archivos

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Literaturas y derechas: Historizar los conceptos, ampliar los archivos Sverdloff, Mariano Javier Analizar a las plurales “literaturas de las derechas” supone dialogar con diversas metodologías y tipos de corpus. Esta diversidad de discursos críticos y archivos implica ir más allá de la close reading de las obras canónicas. Por tanto, al momento de pensar la articulación del clivaje derecha-izquierda con la literatura, la crítica literaria se enfrenta con una doble exigencia: por un lado, debe historizar las diversas perspectivas críticas del siglo XX y XXI sobre las “literaturas de las derechas”; por el otro, debe ampliar los archivos para incluir textos no literarios.; Analyzing differents “right-wing literatures” supposes a dialogue with various methodologies and types of corpus. This diversity of critical discourses and archives implies going beyond close reading of canonical works. Therefore, when thinking about the articulation between the right-left cleavage and literature, literary criticism is faced with a double requirement: on the one hand, to historicize the various critical perspectives of the 20th and 21st centuries on “right-wing literatures”; on the other, to expand the archives and to include non-literary texts.

Wave separation analysis-derived indexes obtained from radial and carotid tonometry in healthy pregnancy and pregnancy-associated hypertension: Comparison with pulse wave analysis-derived indexes

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Wave separation analysis-derived indexes obtained from radial and carotid tonometry in healthy pregnancy and pregnancy-associated hypertension: Comparison with pulse wave analysis-derived indexes Pereira, María M.; Torrado, Juan; Bock, Joshua; Sosa, Claudio; Diaz, Alberto Alejandro; Bia, Daniel; Zócalo, Yanina Background: Increased wave reflections assessed by pulse wave analysis (PWA) was proposed as one of the potential culprits of hypertension seen in women with pregnancy-associated hypertension (PAH). However, this statement has never been confirmed with “Wave Separation Analysis” (WSA), a more sophisticated mathematical approach that analyzes the amplitude and interaction between forward and backward aortic pressure waveform components. Objective: To characterize potential changes in pressure wave components of PAH compared to healthy non-pregnant (NP) women and women with normal pregnancies (HP) by using WSA and compared these findings with PWA-derived indexes; secondarily, to evaluate differences in WSA-derived indexes between subgroups of PAH (i.e., preeclampsia [PE] and gestational hypertension [GH]). Methods: Using radial and carotid applanation tonometry, we quantified in HP (n = 10), PAH (n = 16), and NP (n = 401): (i) PWA-derived indexes; (ii) WSA-derived indexes: forward (Pf) and backward (Pb) waveform components, backward component arrival time (PbAT), reflection magnitude (RM = Pb/Pf) and index [RIx = Pb/(Pf + Pb)]. Results: While PAH was associated with a higher Pf compared to HP and NP, Pb and PbAT were similar between the groups. Both GH and PE showed a higher Pf compared to HP, but only PE had a trend of presenting with higher Pb and lower PbAT compared to the other groups. Finally, PAH showed a trend of having lower RM and RIx compared to NP and HP, with no differences between GH and PE. Conclusion: PAH was associated with higher Pf, but not higher Pb, compared to NP and HP, although PE also demonstrated a trend of higher Pb.

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Suscribirse a Facultad de Ciencias Químicas de la Universidad Nacional de Córdoba agregador - Ciencia y Tecnología