Glaciomarine sequence stratigraphy in the Mississippian Río Blanco Basin, Argentina, southwestern Gondwana: Basin analysis and palaeoclimatic implications for the Late Paleozoic Ice Age during the Tournaisian Ezpeleta, Miguel; Rustán, Juan José; Balseiro, Diego; Davila, Federico Miguel; Dahlquist, Juan Andrés; Vaccari, Norberto Emilio; Sterren, Andrea Fabiana; Prestianni, Cyrille; Cisterna, Gabriela Adriana; Basei, Miguel The Late Paleozoic Ice Age (LPIA) has been well recorded in the uppermost Mississippian?Pennsylvanian of Gondwana. Nevertheless, little is known about the temporal and geographic dynamics, particularly during the early Mississippian. We report on exceptional Tournaisian glaciomarine stratigraphic sections from central Argentina (Río Blanco Basin). Encompassing c. 1400 m, these successions contain conspicuous glacigenic strata with age constraints provided bypalaeontological data and U/Pb detrital zircon age spectra. A variety of marine, glaciomarine and fan-deltaic environments indicate relative sea-level variations mainly associated with tectonism and repetitive cycles of glacial activity. Provenance analysis indicates a source from the Sierras Pampeanas basement located to the east. Fifteen sequences were grouped into threedepositional models: (1) Transgressive Systems Tracts (TST) to Highstand Systems Tracts (HST) sequences unaffected by glacial ice; (2) Lowstand Systems Tracts (LST) to TST and then to HST with glacial influence; and (3) non-glacial Falling-Stage Systems Tracts (FSST) to TST and HST. The glacial evidence indicates that the oldest Mississippian glacial stage of the LPIA in southwestern Gondwana is constrained to the middle Tournaisian. In contrast with previous descriptions of Gondwanan coeval glacial records, our sequence analysis confirms complex hierarchical climate variability, rather than a single episode of ice advance and retreat.

Increased expression of autophagy protein LC3 in two patients with progressing chronic lymphocytic leukemia Arroyo, Daniela Soledad; Rodríguez, Cecilia Inés; Bussi, Claudio; Manzone Rodriguez, Clarisa; Sastre, Darío; Heller, Viviana; Stanganelli, Carmen Graciela; Slavutsky, Irma Rosa; Iribarren, Pablo Chronic lymphocytic leukemia (CLL) is the most common type of adult leukemia in the western hemisphere. It is characterized by a clonal proliferation of a population of CD5+ B lymphocytes that accumulate in the secondary lymphoid tissues, bone marrow, and blood. Some CLL patients remain free of symptoms for decades, whereas others rapidly become symptomatic or develop high-risk disease. Studying autophagy, which may modulate key protein expression and cell survival, may be important to the search for novel prognostic factors and molecules. Here, we applied flow cytometry technology to simultaneously detect autophagy protein LC3B with classical phenotypical markers used for the identification of tumoral CLL B cell clones. We found that two patients with progressing CLL showed increased expression of the autophagy protein LC3B, in addition to positive expression of CD38 and ZAP70 and unmutated status of IGHV. Our data suggest that activation of autophagy flux may correlate with CLL progression even before Ibrutinib treatment.

Early eocene spore and pollen assemblages from the laguna del hunco fossil lake beds, patagonia, argentina Barreda, Viviana Dora; Zamaloa, María del Carmen; Gandolfo, María A.; Jaramillo, Carlos; Wilf, Peter Premise of research. The early Eocene Laguna del Hunco (LH) fossil site, northwestern Chubut Province, Argentina, holds one of the best-preserved and most diverse paleofloras worldwide. The paleoflora comprises ferns, gymnosperms, and flowering plants. Despite the rapidly growing knowledge of its macrofossil record, little is known about the site’s palynological content. Herein, we present the first dispersed spore-pollen assemblages recovered from LH. Methodology. Palynological samples were collected from seven stratigraphic levels of the Tufolitas de LH (Huitrera Formation), of which six yielded palynomorphs. We determine the botanical affinities of fossil morphotypes and provide systematic descriptions of some taxa. In addition, we establish similarities between LH and other Patagonian Eocene localities, and we compare palynological and prior megafloral records from LH. Pivotal results. We identify 56 spore and pollen species and 28 plant families, of which eight (Cyatheaceae, Schizaeaceae, Polypodiaceae, Asteraceae, Chloranthaceae, Nothofagaceae, Rubiaceae, and Ulmaceae) are reliably reported from the site for the first time. Among other Eocene Patagonian palynofloras, the LH assemblage is similar to Pampa de Jones/Nahuel Huapi Este and Confluencia. Conclusions. The LH spore-pollen assemblages augment the plant fossil record for this significant Eocene locality by incorporating new taxa (e.g., Asteraceae, with one morphotype that represents the oldest record of the family in Patagonia). The new data also reinforce the presence of plant families previously reported from macrofossils, such as Juglandaceae, with pollen grains similar to those of the Engelhardia-Alfaroa group, and Fagaceae (Castaneoideae), complementing the macrofossil record of leaves and reproductive structures.

Economías sexoafectivas: significados, prácticas y relaciones en tensión en contextos petroleros de la Patagonia Argentina; Sexual-affective economies: meanings, practices, and relationships in tension in oil industry contexts of Argentine Patagonia Cabrapan Duarte, Melisa Gisel En este artículo, se analiza la configuración de las economías sexoafectivas en torno a la prostitución en contextos petroleros de la Patagonia argentina. Además, se estudian las diferentes dinámicas conyugales y familiares entre hombres y mujeres que se vincularon sexoafectivamente a través del mercado sexual o fuera de él, y que desenvuelven y sostienen económicamente sus vidas en contextos dependientes de la actividad hidrocarburífera. El objetivo es, a partir de las experiencias y discursos de diferentes actores, indagar en cómo se construye y significa la cotidianidad de esos relacionamientos, y el modo en que se expresan las tensiones entre la sexualidad, el afecto y la economía.; This article analyses the conformation of the sexual-affective economies around prostitution in oil industry contexts of Argentine Patagonia. Likewise, it studies different conjugal and family dynamics, between men and women that were involved sexual-affectively through the sex-trade context or outside of it, and who developed and sustained their lives in a local economy that is dependent on the oil and gas industry. The purpose is, based on the experiences and discourses of different actors, to analyze hoy everyday life is built in those relationships, what do they mean and how the tensions between sexuality, affection, and the economy are expressed.

Influencia del método de dispersión en el fraccionamiento físico de un suelo de Argentina central; Four dispersion methods, typically applied to soil physical fractionation protocols, were compared in a Mollisol from mountain grasslands from central Argentina. The proportion of coarse and fine soil fractions obtained after dispersion was significantly different. Considering that mineral fractions after dispersion using glass beads did not differ from granulometric analysis, this method achieved better results than sonication or shaking with chemical dispersant for evaluated soils. Pestoni, Sofía; Gallardo, Norma; Pérez Harguindeguy, Natalia; Kowaljow, Esteban Se compararon cuatro métodos de dispersión de suelos, ampliamente utilizados en protocolos de fraccionamiento físico, sobre un Molisol de pastizales montañosos del centro de Argentina. La proporción de las fracciones fina y gruesa del suelo varió significativamente entre métodos. Debido a que las fracciones minerales post dispersión con bolitas de vidrio no difirieron del resultado del análisis granulométrico, este método mostró los mejores resultados, por sobre la sonicación y el agitado con dispersante químico, para los suelos evaluados.

Crustal seismicity in the Andean Precordillera of Argentina using seismic broadband data Venerdini, Agostina Lia; Alvarado, Patricia Monica; Ammirati, Jean Baptiste; Podesta Di Santo, Marcos Gaston; López, Luciana; Fuentes, Facundo; Linkimer, Lepolt; Beck, Susan In this study, we analyze 100 crustal Precordilleran earthquakes recorded in 2008 and 2009 by 52 broadbandseismic stations from the SIEMBRA and ESP, two temporary experiments deployed in the Pampeanflat slabregion, between the Andean Cordillera and the Sierras Pampeanas in the Argentine Andean backarc region.In order to determine more accurate hypocenters, focal mechanisms and regional stress orientations, werelocated 100 earthquakes using the JHD technique and a local velocity model. The focal depths of our relocatedevents vary between 6 and 50 km. We estimated local magnitudes between 0.4≤ML≤5.3 and momentmagnitudes between 1.3≤Mw≤5.3. Focal mechanisms were determined from new hypocenter relocations andfirst motion P-wave polarities. Our solutions exhibit a majority of the earthquakes with reverse faulting me-chanism. Regional stress tensor from the inversion of P- and T-axis orientations, shows a maximum stress axis(σ1) almost horizontal with a strike of 85° and a minimum stress axis (σ3) almost vertical.We correlate this small-to-moderate magnitude seismicity with the presence of large basement structuresbeneath the Iglesia-Calingasta Basin in the west and the Eastern Precordillera in the east. The nucleation of deepearthquakes beneath the Iglesia Basin could be related to the presence of a major ramp accommodating thecrustal shortening between the Frontal Cordillera and the Precordillera. The crustal seismicity beneath thePrecordillera seems to correlate with west-dipping structures rooting deep into the Cuyania basement suggestinga thick-skinned basement deformation system beneath the Precordillera and its shallow thin-skinned fold andthrust belt.

Delayed Bilateral Teleoperation of the Speed and Turn Angle of a Bipedal Robot Moya, Viviana; Slawiñski, Emanuel; Mut, Vicente Antonio This paper proposes a shared control scheme which aims to achieve a stable control of the speed andturn of a bipedal robot during a delayed bilateral teleoperation. The strategy allows to get a delay-dependent damping value that must be injected to assure a bounded response of the hybrid system,while simultaneously, the human operator receives a force feedback that help him to decrease thesynchronism error. Furthermore, a test where a human operator handles the walking of a simulatedbipedal robot, to follow a curve path in front of varying time delay, is performed and analyzed.

Canonical transient receptor potential 6 channel deficiency promotes smooth muscle cells dedifferentiation and increased proliferation after arterial injury Smith, Andrew H.; Priya, Bhanu Priya; Driscoll, Erin C.; Chaudhuri, Pinaki; Birnbaumer, Lutz; Rosenbaum, Michael A.; Graham, Linda M. Objective Previous studies showed the benefit of canonical transient receptor potential 6 (TRPC6) channel deficiency in promoting endothelial healing of arterial injuries in hypercholesterolemic animals. Long-term studies utilizing a carotid wire-injury model were undertaken in wild-type (WT) and TRPC6-/- mice to determine the effects of TRPC6 on phenotypic modulation of vascular smooth muscle cells (SMC) and neointimal hyperplasia. We hypothesized that TRPC6 was essential in the maintenance or reexpression of a differentiated SMC phenotype and minimized luminal stenosis following arterial injury. Methods The common carotid arteries (CCA) of WT and TRPC6-/- mice were evaluated at baseline and 4 weeks after wire injury. At baseline, CCA of TRPC6-/- mice had reduced staining of MYH11 and SM22, fewer elastin lamina, luminal dilation, and wall thinning. After carotid wire injury, TRPC6-/- mice developed significantly more pronounced luminal stenosis compared with WT mice. Injured TRPC6-/- CCA demonstrated increased medial/intimal cell number and active cell proliferation when compared with WT CCA. Immunohistochemistry suggested that expression of contractile biomarkers in medial SMC were essentially at baseline levels in WT CCA at 28 days after wire injury. By contrast, at 28 days after injury medial SMC from TRPC6-/- CCA showed a significant decrease in the expression of contractile biomarkers relative to baseline levels. To assess the role of TRPC6 in systemic arterial SMC phenotype modulation, SMC were harvested from thoracic aortae of WT and TRPC6-/- mice and were characterized. TRPC6-/- SMC showed enhanced proliferation and migration in response to serum stimulation. Expression of contractile phenotype biomarkers, MYH11 and SM22, was attenuated in TRPC6-/- SMC. siRNA-mediated TRPC6 deficiency inhibited contractile biomarker expression in a mouse SMC line. Conclusions These results suggest that TRPC6 contributes to the restoration or maintenance of arterial SMC contractile phenotype following injury. Understanding the role of TRPC6 in phenotypic modulation may lead to mechanism-based therapies for attenuation of IH. Clinical Relevance After endovascular intervention and open vascular surgery, vascular smooth muscle cells (VSMC) undergo a coordinated reprogramming of gene expression to facilitate arterial healing. Down regulation of VSMC-specific contractile biomarkers (eg, SM22 and MYH11) and induction of pathways that promote cell proliferation, migration, and matrix synthesis are hallmarks of this phenotypic switch. Dysregulated phenotypic switching leads to the development of neointimal hyperplasia and vascular restenosis. Identifying pathways that regulate or constrain VSMC phenotypic modulation, therefore, has the potential to decrease neointimal hyperplasia and improve outcomes after vascular intervention. In this study, we demonstrate that depletion of the non-voltage-gated cation channel TRPC6 promotes phenotypic switching and loss of contractile biomarkers in systemic arterial VSMC. TRPC6-/- mice developed significantly more pronounced luminal stenosis compared with wild-type mice after carotid wire injury. These results suggest that TRPC6 contributes to the restoration or maintenance of contractile phenotype in VSMC after injury. Understanding the role of TRPC6 in phenotypic switching may lead to mechanism-based therapies to mitigate restenosis.

Escuelas rurales de alternancia y cogestión: Un análisis sociolingüístico y etnográfico de las tomas de decisiones en el Consejo de Administración; Alternation Rural Schools and Co-management: A Sociolinguistic and Ethnographic Framework to Analyzing Decision Making at the Board Miano, María Amalia; Lara Corro, Erik Said; Heras, Ana Inés Realizamos un análisis sociolingüístico y etnográfico de las interacciones que tienen lugar entre los miembros de un Consejo de Administración de una escuela rural de alternancia. Este Consejo está formado por padres y madres de los estudiantes, egresados y docentes que se reúnen para tomar decisiones sobre la gestión de la escuela. Se trata de actores que ocupan lugares diferenciados en la estructura organizacional y que portan recursos materiales, culturales y simbólicos diversos. A través del análisis de las interacciones sostenemos que los procesos de tomas de decisión exceden los temas vinculados a la gestión de lo escolar para abarcar las situaciones de vida de las familias que habitan en el medio rural. Estos procesos implican para los Consejeros el aprendizaje de una competencia comunicativa vinculada a ejercer una práctica de escucha, ponderación, debate y seguimiento de la decisión alcanzada.; We present a sociolinguistic and ethnographic analysis of interactions taking place at the Board of a rural school run by the pedagogy of alternance model. The members of the Board are the students´ family, teachers, the principal and alumni, who meet in order to make decisions together. In as much as these members occupy different and differential positions in the school, their material and symbolic resources are diverse. Our analysis shows that the processes entailed in making decisions relate not only to the specific issues brought to discussion but also to several other situations connected to the way in which the members of the rural community live. Therefore, making decisions at the Board involves developing a specific communicative competence related to learning to listen, ponder alternatives, engage in debate, and follow up with the decision taken as a collective.

Territorializar la extensión universitaria: diálogo de saberes y co-construcción de conocimiento Valente Ezcurra, Dana; Girado, Agustina; Migueltorena, Alejandro En el presente artículo abordamos las discusiones implicadas en la territorialización de la universidad, profundizando en el rol de la extensión universitaria y la función social del conocimiento. Estas reflexiones se han nutrido de las experiencias de trabajo realizadas colectivamente en el marco de distintos proyectos de extensión, que permitieron articular diversas intervenciones y acciones vinculadas al Acceso Justo al Hábitat y el Derecho a la Ciudad en Tandil (Buenos Aires, Argentina) desde el año 2013 a la actualidad. Con el objetivo de relacionar diversos enfoques y prácticas es que nos preguntamos cómo territorializar la universidad; atendiendo a las respuestas que suscita este interrogante la estructura argumental se ordena en tres apartados. El primero de ellos aborda los debates en torno a las perspectivas desde las que se conceptualiza la Extensión Universitaria y su función social, profundizando en sus implicancias respecto a la construcción de territorios y territorialidades. El segundo, recupera la función social del conocimiento, discutiendo los requerimientos ontológicos, epistemológicos y metodológicos exigidos para el diálogo de saberes y la co-construcción de conocimiento. Finalmente, compartimos algunas reflexiones situadas sobre nuestras experiencias de trabajo, pretendiendo contribuir a la construcción de prácticas integrales, que desde el territorio y junto a organizaciones sociales avancen en la transformación de las desigualdades con un horizonte de justicia social y espacial.

Trayectorias y dinámicas de adultos/as mayores: curso de vida y alimentación en diálogo; Trajectories and food dynamics of Elderly People: food as life story Abraham, Maria Daniela; Huergo, Juliana; Butinof, Mariana Argentina atraviesa un proceso de transición demográfica avanzada, ya que los/as Adultos Mayores (AM) alcanzan el 15,1% de la población. Ello pone en tensión el lugar de las/os AM en la sociedad, sus condiciones de acceso y resolución de necesidades, entre ellas la alimentación. En este trabajo se postula que hablar de la alimentación, de los alimentos, es hablar de los/as AM, de la historia de vida de estas personas que se va escribiendo entre sustancias y circunstancias que hacen a actividades tan cotidianas como el cocinar y el comer. Así, se planteó como objetivo, indagar acerca de la categoría trayectorias y dinámicas alimentarias (TyDA) de los/as AM de la Ciudad de Córdoba-Argentina, para establecer diálogo(s) posible(s) entre alimentación y curso de vida.Se realizó una investigación con posicionamiento epistemológico mixto entre paradigma interpretativista y crítico; con triangulación metodológica cuali-cuantitativa. Etapa cuantitativa: muestra no probabilística por cuotas según nivel de instrucción (n=384); incluyendo a AM de Córdoba y gran Córdoba que integran espacios participativos. Se implementó un cuestionario semiestructurado. Etapa cualitativa: muestra intencional (n=10) a partir del muestro anterior. Se realizaron observaciones participantes, bitácora de campo y entrevistas. Participaron 384 AM, edad promedio de 72 años (DE=7), 79% mujeres y 21% varones. Las TyDA, siguiendo una idea de proceso que se construye y reconstruye constantemente, fueron abordadas desde: a) Etapa de la vida b) Sistema Alimentario y c) Alimentos identificadores. Reconstruir la historia de la comida de los/as AM propicia el reencuentro con la propia historia (infancia, juventud, adultez, vejez). Esta matriz de sentido es una invitación a repensar los abordajes en materia de políticas públicas destinados a este grupo poblacional; que genera cambios demográficos tanto a nivel macrosocial (cultura, economía, mercado de trabajo, sistemas políticos) pero especialmente en la experiencia de vida de los y las AM.; Argentina is going through an advanced demographic transition process, as the Elderly (E) reach 15.1% of the population. This puts in strain the place of the E in society, their conditions of access and resolution of needs, including food. In this work it is postulated that to talk about food is to talk about the E, about the life story of these people that is being written among substances and circumstances resulting from daily activities such as cooking and eating. Thus, the objective was to research on trajectories and food dynamics (T&FD) of the E living in Cordoba City, Argentina, to establish possible dialogue(s) between food and life course. An investigation with mixed epistemological positioning between interpretive and critical paradigm was carried out; with qualitative quantitative methodological triangulation. Quantitative stage: non-probabilistic sample according to level of instruction (n=384); including the E from Cordoba and great Cordoba that integrate participatory spaces. A semistructured questionnaire was implemented. Qualitative stage: intentional sample (n=10) resulting from the previous sample. Participant observations, field log and interviews were made. 384 participated, average age of 72 years old (SD=7), 79% women and 21% men. The T&FD, following a process idea that is constantly constructed and reconstructed, were approached from: a) Life stage b) Food System and c) Food identifiers. Reconstructing the history of the food of the E favors the connection with their own life story (childhood, youth, adulthood, old age). This matrix of meaning is an invitation to rethink public policy approaches for this population group, which generates demographic changes at a macrosocial level (culture, economy, labor market, political systems) but especially in the life experience of these E.

HoSeIn: A Workflow for Integrating Various Homology Search Results from Metagenomic and Metatranscriptomic Sequence Datasets Rozadilla, Gastón; Moreiras Clemente, Jorgelina; Mccarthy, Cristina Beryl Data generated by metagenomic and metatranscriptomic experiments is both enormous and inherently noisy. When using taxonomy-dependent alignment-based methods to classify and label reads, the first step consists in performing homology searches against sequence databases. To obtain the most information from the samples, nucleotide sequences are usually compared to various databases (nucleotide and protein) using local sequence aligners such as BLASTN and BLASTX. Nevertheless, the analysis and integration of these results can be problematic because the outputs from these searches usually show inconsistencies, which can be notorious when working with RNA-seq. Moreover, and to the best of our knowledge, existing tools do not criss-cross and integrate information from the different homology searches, but provide the results of each analysis separately. We developed the HoSeIn workflow to intersect the information from these homology searches, and then determine the taxonomic and functional profile of the sample using this integrated information. The workflow is based on the assumption that the sequences that correspond to a certain taxon are composed of:1)sequences that were assigned to the same taxon by both homology searches;2)sequences that were assigned to that taxon by one of the homology searches but returned no hits in the other one.

Rab Proteins: Insights into Intracellular Trafficking in Endometrium Leiva, Natalia Lorena; Nolly, Mariela Beatriz; Ávila Maniero, Mariángeles; Losinno, Antonella Denise; Damiani, Maria Teresa Rab proteins belong to the Ras superfamily of small monomeric GTPases. These G proteins are the main controllers of vesicular transport in every tissue, among them, the endometrium. They are in charge of to the functional subcellular compartmentalization and cargo transport between organelles and the plasma membrane. In turn, intracellular trafficking contributes to endometrial changes during the menstrual cycle, secretion to the uterine fluid, and trophoblast implantation; however, few reports analyze the role of Rab proteins in the uterus. In general, Rab proteins control the release of cytokines, growth factors, enzymes, hormones, cell adhesion molecules, and mucus. Further, the secretion of multiple compounds into the uterine cavity is required for successful implantation. Therefore, alterations in Rab-controlled intracellular transport likely impair secretory processes to the uterine fluid that may correlate with abnormal endometrial development and failed reproductive outcomes. Overall, they could explain recurrent miscarriages, female infertility, and/or assisted reproductive failure. Interestingly, estrogen (E2) and progesterone (P) regulate gene expression of Rab proteins involved in secretory pathways. This review aims to gather information regarding the role of Rab proteins and intracellular trafficking in the endometrium during the different menstrual phases, and in the generation of a receptive stage for embryo implantation, modulated by E2 and P. This knowledge might be useful for the development of novel reproductive therapies that overcome low implantation rates of assisted reproductive procedures.

The Effect of different vitrification and staining protocols on the visibility of the nuclear maturation stage of equine oocytes Pereira, Blasa C.; Ortiz, Isabel; Dorado, Jesús; Diaz Jimenez, Maria; Consuegra, Cesar; Demyda Peyrás, Sebastián; Hidalgo, Manuel In this study, we compared two staining protocols assessing the nuclear chromatin stage of equine oocytes after vitrification using permeable and nonpermeable cryoprotectants. Slaughterhouse-derived oocytes (n = 155) were obtained from a total of 32 mares and in vitro matured in M199 medium for 42 hours at 38.5°C in 5% CO2. In the first experiment, two concentrations of Hoechst 33342 (HO) were tested (10 μg/mL; P1 and 2.5 μg/mL; P2) combined with 50 μg/mL of propidium iodide as staining protocols to evaluate the visibility of matured oocytes (n = 44). In the second experiment, 111 oocytes were evaluated using the staining protocol P2, before (C, control) and after vitrification following a two-step conventional protocol with (15% dimethyl sulfoxide, 15% ethylene glycol, and 0.5 M sucrose; V1) or without (1 M sucrose; V2) using permeable cryoprotectants. Our results showed that P2 provided a higher percentage of oocytes with outstanding visibility of the nuclear chromatin stage (52.17%; P < .05) in comparison with P1 (19.04%). In the second experiment, no cryoprotectant-free vitrified oocytes reached the metaphase II maturation stage. This result was significantly lower (P < .05) than conventional vitrification (15.38%) and both lower in comparison with the nonvitrified control group (42.11%). In conclusion, permeable cryoprotectant-free vitrification of equine oocytes obtained poor results and therefore cannot be considered an alternative to vitrification using permeable cryoprotectants. In addition, a staining protocol with a low concentration of HO is recommended to evaluate the nuclear chromatin stage of equine oocytes after in vitro maturation.

First study of food webs in a large glacial river: the trophic role of invasive trout Tagliaferro, Marina Beatriz; Kelly, Sean P.; Pascual, Miguel Alberto El objetivo de este estudio fue determinar la estructura trófica de un gran río de la Patagonia en dos secciones (río arriba y medio) y evaluar la superposición isotópica entre especies nativas e introducidas. Utilizamos análisis de isótopos estables δ15N y δ13C y contenido estomacal. La sección río arriba tuvo una estructura de trama trófica más compleja, con mayor riqueza de macroinvertebrados y peces respecto de la sección media. Los recursos basales dominantes río arriba fueron las algas filamentosas. En esta área, la trucha de lago tuvo la posición trófica más alta entre los peces, aunque, las especies de peces más abundantes fueron las truchas arcoiris. Dependiendo del estadio, la trucha arcoiris cambió su rol de presa a competidor/depredador. En la sección media del río, la base de la trama trófica estuvo dominada por materia orgánica particulada gruesa y la trucha arcoíris adulta fue el depredador tope. Los valores isotópicos variaron entre zonas para invertebrados y peces. Las dos especies nativas e invasoras más abundantes, Puyen y trucha arcoiris, mostraron una separación isotópica en la sección media, pero no en secciones de río arriba. La presencia de peces invasores que ocupan una posición tope en los niveles tróficos puede tener un impacto significativo sobre las poblaciones de peces nativos de gran importancia ecológica en la región; The aim of this study was to determine the food webs structure of a large Patagonian river in two river sections (Upstream and Midstream) and to evaluate isotopic overlap between native and introduced species. We used stable isotope analyses of δ15N and δ13C and stomach content. The Upstream section had a more complex food webs structure with a greater richness of macroinvertebrates and fish species than Midstream. Upstream basal resources were dominated by filamentous algae. Lake Trout were found to have a higher trophic position than all other fish species in that area although, the most abundant fish species, were Rainbow Trout. Depending on the life stage, Rainbow Trout shifted from prey to competitor/predator. In the Midstream section, the base of the food webs was dominated by coarse particulate organic matter, and adult Rainbow Trout had the highest trophic level. Isotopic values changed among macroinvertebrates and fish for both areas. The two most abundant native and invasive species — Puyen and Rainbow Trout — showed an isotopic separation in Midstream but did not in Upstream areas. The presence of invasive fish that occupy top trophic levels can have a significant impact on native fish populations that have great ecological importance in the region.

Configuración y montaje de instrumento para la caracterización magnetoeléctrica de compuestos cerámicos multiferroicos Camargo, Javier Eduardo; Lere, Martin Enrique; Castro, Miriam Susana; Ramajo, Leandro Alfredo Este trabajo presenta la configuración y montaje de un equipo empleado para la cuantificar el coeficiente magnetoeléctrico dinámico (α) de compuestos cerámicos multiferroicos. En particular, se analizan las propiedades magnetoeléctricas de los compuestos de composición Bi0.5(Na0.8K0.2)0.5TiO3-Ni0.5Co0.5Fe2O4 (BNKT-NCF). En el método dinámico, la señal magnetoeléctrica (ME) es registrada midiendo el potencial eléctrico a través de la muestra bajo un campo magnético continuo y variable en presencia de un campo magnético de CA. Los elementos de medición constan de un sistema de polarización (eléctrico y magnético), para polarizar la cerámica magnetoeléctrica, y un lock-in para generar la señal alterna y filtrar el ruido. Además, dada la baja señal ME es necesario un blindaje adecuado y una sección fina de electrodos junto al amplificador tipo lock-in. El rendimiento de los dispositivos fabricados resulta satisfactorio para realizar la medición dinámica del efecto ME para materiales magnetoeléctricos basados en cerámicos multiferroicos.; In the present work, the configuration and assembly of a device used to quantify the dynamic magnetoelectric coefficient (®) of multiferroic lead-free ceramic is analyzed. In particular, the magnetoelectric properties of Bi0,5(Na0,8K0,2)0,5TiO3-Ni0,5Co0,5Fe2O4 (BNKT-NCF) ceramics are studied. In the dynamic method, the magnetoelectric signal (ME) is recorded measuring the electrical potential across the sample under a DC magnetic field in the presence of an AC magnetic field. The measuring elements consist of a polarization system (electric and magnetic), to polarize the magnetoelectric ceramics, and a lock-in to generate the alternating signal and filter out the noise. Besides due to the low ME signal, adequate shielding and a thin electrode section are necessary close to the lock-in amplifier. The performance of the manufactured devices is satisfactory to perform the dynamic measurement of the ME effect for magnetoelectric materials based on multiferroic ceramics.

Low-quality management of Marine Protected Areas in the North-East Atlantic Álvarez-Fernández, Inmaculada; Freire, Juan; Sanchez Carnero, Noela Belen The effectiveness of a marine protected area (MPA) requires adequate management plans (MgPs), good man-agement performance and effective governance. This study diagnosed management quality on 126 MPAs and 57 MgPs in four countries in the NE Atlantic Ocean (France, England, Spain and Portugal) by adapting the MPA Management Effectiveness Assessment Tool to assess management quality, and management effort, strengths and weaknesses. Despite the fact that these MPAs were, in 2013, on average 14-year old, fewer than 30% showed sufficient management capacity, 18% showed operational management, and only 3% showed effective management. Therefore, 70% could be considered unmanaged or “paper” reserves. Among their strengths, MPAs were in general well legislated in the four countries and had operational management bodies in France and England. However three key factors need to be improved in all countries: (i) periodic MgP updated; (ii) periodic MPA monitoring and assessment; and (iii) education and communication activities. Enforcement should be improved in England, France and Portugal; Portugal and Spain also need to improve the management body and England and Portugal the community participation.

Coming to light: First insight into the genetic diversity of Peale's dolphins at a Patagonian MPA Durante, Cristian Alberto; Cunha, Haydée Andrade; Crespo, Enrique Alberto; Loizaga de Castro, Rocio Genetic diversity is a measure of genetic variation among individuals of a species. This variation, a result of mutations and changes in allelic frequencies, can be maintained or not due to the action of other evolutionary forces, namely migration (gene flow), genetic drift, and natural selection (Frankham, Ballou, & Briscoe, 2002). Genetic diversity is the raw matter upon which natural selection operates, providing species with the capacity to cope with habitat changes, i.e., their evolutionary potential (Laikre, 2010). For this reason, the preservation of genetic diversity is considered an essential issue by the International Union for Conservation of Nature (McNeely, Miller, Reid, Mittermeier, & Werner, 1990; Willoughby et al., 2015), and genetic studies provide essential data for the conservation of biodiversity (Laikre, 2010).

First record of clinical coccidiosis (Eimeria ovinoidalis) in adult sheep from northwestern Argentina Olmos, Leandro Hipolito; Colque Caro, Luis Adrián; Avellaneda Cáceres, A.; Medina, D. M.; Sandoval, Gabriela Virginia; Aguirre, Héctor David; Micheloud, Juan Francisco Coccidiosis of sheep is an intestinal infection caused by protozoa of the genus Eimeria. An outbreak of the disease in adult sheep from Salta province, northwestern Argentina, was studied to establish its clinical, epidemiological, pathological and etiological aspects. The affected animals were part of a flock of 20 sheep brought from Formosa province about 10 days before. Most sheep (80% incidence) showed hemorrhagic diarrhea, dehydration and loss of body condition; six of them died and two that became permanently recumbent were euthanized. Three necropsied sheep showed mild mesenteric lymphadenomegaly, diffuse proliferative enteritis in the small and large intestines, and mucosal thickening. Histopathological studies exhibited diffuse proliferative enteritis and presence of structures compatible with intracellular coccidia at different stages of development. Parasitological studies (n = 12) resulted in an average of 16,636.6 (± 15,266.8) Eimeria oocysts per gram of feces (range 1680-46,400). Taxonomy of Eimeria species based on analysis of sporulated oocysts derived from 4 fecal samples (n = 100 oocyst per sample) showed, on average, a high prevalence of E. ovinoidalis (61.5%), followed by E. parva (27.2%), and lower proportions of E. crandallis (5.3%), E. ahsata (3.2%) and E. intricata (2.8%). Clinical and pathological findings confirmed the diagnosis of coccidiosis in the affected sheep; parasitological results showed that E. ovinoidalis was the main species responsible for the clinical signs. Clinical coccidiosis is considered unusual in adult sheep, but the present case shows that under favorable environmental and/or management conditions, this infection may be highly deleterious for adult sheep.

On the catalytic activation of water-soluble NHC-Au(I) complexes by sonication and microwave irradiation: A comparative assessment Fernandez, Gabriela Araceli; Schiel, María Ayelén; Silbestri, Gustavo Fabián Within the realm of sustainable chemistry and increasing demand for eco-compatible methods, this article describes our attempts to activate some water-soluble N-heterocyclic carbene (NHC)-based gold(I) complexes under two well-known and safe irradiation protocols: microwaves (MW) and ultrasound (US). A textbook reaction, alkyne hydration, was chosen to assess the performance of such activation protocols and their influence on the catalyst's ability to drive the reaction to completion. Reactions can easily be conducted in pure water or aqueous methanol (1:1). We show that sonication significantly enhances this transformation affording the product in higher yields and shorter reaction times than non-assisted reactions in water, while MW energy gives the desire product in quantitative yield in water:methanol after just 1 min.