El maná de una particular energía. El panorama de las instalaciones hipersaturadas de Diego Bianchi y Leopoldo Estol a mediados del dos mil Baeza, Federico Aníbal Mariano Diego Bianchi y Leopoldo Estol, luego de conocerse en la beca Kuitca, trabajaron juntos una sola vez, el resultado fue la instalación Escuelita Thomas Hirschhorn en 2005. En la galería Belleza y Felicidad montaron una gruta tapizada con la característica cinta marrón de embalar –la reconocida marca del artista suizo– dando forma a una serie de claustrofóbicos ambientes interconectados por desiguales funciones: kiosco, ciber-locutorio, disco, aula, mirador y jardín. Este espacio multiforme albergó fiestas, clases, charlas, entre otros encuentros que discurrieron en los calurosos últimos días del año.

Molecular gas in the star-forming region IRAS 08589-7414 Saldaño, Hugo Pablo; Vasquez, Javier; Cappa, Cristina Elisabeth; Gomez, Mercedes Nieves; Duronea, Nicolas Urbano; Rubio, M. Aims. We present an analysis of the region IRAS 08589−4714 with the aim of characterizing the molecular environment. Methods. We observed the 12CO(3 −2), 13CO(3 −2), C18O(3 −2), HCO+(3 −2), and HCN(3 −2) molecular lines in a region of 150′′× 150′′, centered on the IRAS source, to analyze the distribution and characteristics of the molecular gas linked to the IRAS source. Results. The molecular gas distribution reveals a molecular clump that is coincident with IRAS 08589−4714 and with a dust clump detected at 1.2 mm. The molecular clump is 0.45 pc in radius and its mass and H2 volume density are 310 M⊙ and 1.2 × 104 cm-3, respectively. Two overdensities were identified within the clump in HCN(3−2) and HCO+(3−2) lines. A comparison of the LTE and virial masses suggests that the clump is collapsing in regions that harbor young stellar objects. An analysis of the molecular lines suggests that they are driving molecular outflows.

Interdependencia económica mundial y procesos de resistencia campesina en un mundo globalizado: La experiencia de la Vía Campesina internacional Pinto, Lucas Henrique En el artículo analizaremos la globalización de la economía mundial y las formasde resistencia campesina generadas desde mediados de los años de 1990,problematizando así la emergencia de conflictos sociales desencadenados porlos procesos de globalización, y la novedad en el ámbito de la acción colectivarural que fue la creación de una organización internacional de campesinos.Analizaremos a La Vía Campesina (LVC), movimiento mundial que pretende nuclearpequeños productores rurales frente al accionar de instituciones como laOrganización Mundial del Comercio en la producción mundial de alimentos. Elobjetivo del trabajo es ver de manera breve cómo la globalización afecta las formasde vida campesinas en el mundo; cómo esos campesinos procuran resistira este proceso organizándose a escala mundial e internacionalizando su lucha.Para ello, examinaremos cómo se da el nacimiento de la organización a partirde documentos, entrevistas y sus cuestionamientos al proceso de globalizacióncapitalista-neoliberal.; In the article discuss the globalization of the world economy and forms of peasant resistance generated since mid-1990 years, problems thus the emergence of social conflicts triggered by globalization processes, and novelty in the field of rural collective action which was the creation of an international organization of peasants. We will analyze at La Via Campesina. (LVC), global movement seeking nuclear small farmers against the actions of institutions such as the World Trade Organization on world food production. The objective of the study is to see briefly how globalization affects rural ways of life in the world; how those farmers seek to resist this process organizing themselves on a global scale and internationalize their struggle. Therefore we will examine how the birth of the organization from documents, interviews and their questions is given to the neoliberal capitalist globalization process.

Trazas de una cultura institucional policial a través de la historia de la Sociedad de Socorros Mutuos de la Policía de la Provincia de Buenos Aires en el siglo XX; Traces of a Police Institutional Culture through the History of the Sociedad de Socorros Mutuos of the Buenos Aires Province Police during the XX Century Barreneche, Osvaldo Este artículo se centra en la historia de la Sociedad de Socorros Mutuos de la Policía de la provincia de Buenos Aires, en especial, durante la primera mitad del siglo XX, señalando que fue en el temprano marco de dicha organización mutualista, siempre vinculada a las jefaturas de policía, donde se gestaron algunos de los fundamentos y prácticas que ayudaron a cimentar un obrar institucional de larga perdurabilidad en el tiempo. El vínculo de semi-autonomía generado entre la Sociedad, la conducción policial y el estado bonaerense, sirvió también de modelo de otras asociaciones integradas por policías y que tendrían su nacimiento y creciente influencia durante la segunda mitad del siglo XX. Precisamente a partir de mediados de dicho siglo comenzó el declinar de la Sociedad, como entidad representativa de los policías, por lo que al final del trabajo se formulan algunas hipótesis de las razones por las cuales esto puede haber ocurrido y el legado de la Sociedad en términos de la consolidación de una cultura institucional policial.; This article deals with the history of the Mutual Aid, Friendly Society of the Buenos Aires Province Police, especially during the first half of the XX Century. The piece argues that it was in the early context of this mutual aid association, always tied to the mentioned Police Agency, where some ideas of how to conduct police business took place. The bond among the Society, the Police Agency, and the Provincial State Government served as influential model for other police associations which were born later on during the second part of the same Century. Precisely from the middle of the Century on the Society began to slowly fade away as a highly representative police association. However, the Society´s symbolic influence never disappeared and in this paper we would formulate some hypothesis about that endurance as well as its connections with the consolidation of a police institutional culture.

Uso de atmósfera controlada y 1-MCP en kiwis ´Hayward´ producidos en Argentina: efectos sobre la maduración Yommi, Alejandra Karina; Baeza, María Cecilia; Casanovas, Elda Mabel; Quillehauquy, Victoria; Borrajo, María Paula; Fasciglione, Gabriela Se estudió el efecto del uso de la atmósfera controlada (AC), 1-metilciclopropeno (1-MCP) y su combinación, sobre la maduración del kiwi ‘Hayward’. Frutos cosechados con 16,6% de materia seca y 9% de sólidos solubles totales (SST) fueron enfriados y tratados con 1000 ppb de 1-MCP (SmartFresh) durante 24h o permanecieron sin tratar con 1-MCP (controles). Posteriormente, frutas controles y tratadas con 1-MCP fueron almacenadas en cámara de frío convencional (FC, 0 °C y 90% HR) o en cámara de atmósfera controlada (AC, 2% O2 y 5% CO2, 0 °C; 90% HR) durante 5 meses (5m). Al finalizar, muestras de todas las combinaciones de tratamiento permanecieron durante un mes adicional en FC (5m + 1m). A la cosecha, a los 5m y 5m + 1m y después de la poscámara (7 días a 20 °C) se midieron el índice de color (en base a L*, a*, b* de CIE) y la firmeza de la pulpa (N), el SST (%) y la acidez titulable (AT, %). También se evaluó la dureza de columela al final de la poscámara, mediante análisis sensorial. Los tratamientos afectaron fundamentalmente la firmeza de la pulpa y de la columela. Los frutos almacenados en AC resultaron significativamente más firmes que los almacenados en FC, tanto a los 5m como a los 5m + 1m, evidenciando el efecto residual de dicha tecnología. Igual resultado se encontró para dureza de columela. El uso de 1-MCP en combinación con la AC también mostró un efecto sinérgico sobre la firmeza a los 5m + 1m, permitiendo prolongar el período de almacenamiento de los frutos.; The effect of the use of controlled atmosphere (CA), 1-methylcyclopropene (1-MCP) and their combination on the ripening of ‘Hayward’ kiwifruits was studied. Fruits harvested with 16.6% dry matter and 9% of total soluble solids (TSS) were cooled and treated with 1000 ppb of 1-MCP (SmartFresh) for 24 hours or remained untreated (controls). Untreated and 1-MCP-treated fruits were kept at cold storage (CS, 0 °C and 90% RH) or at controlled atmosphere storage (CA, 2% O2 and 5% CO2, 0 °C and 90% RH) for 5 months (5m). Subsequently, samples from all treatment combinations remained for an additional month at CS (5m + 1m). At harvest, 5m and 5m+1m and shelf-life (7 days at 20 °C), color index (based on L*, a*, b* of CIE) and flesh firmness (N), TSS (%) and titratable acidity (TA, %) were measured. Hard core was also evaluated by sensory analysis at shelf-life. Treatments mainly affected the flesh firmness and hard core. Fruits stored in CA were significantly firmer than those stored in CS, both at 5m and 5m+1m, showing the residual effect of this technology. The same result was found for hard core. The use of 1-MCP in combination with CA also showed a synergistic effect on flesh firmness at 5m + 1m, prolonging the storage period of the fruits.

Peronismo para la Liberación Nacional ¿y social? El vínculo peronismo-revolución en las revistas Militancia Peronista para la Liberación y Envido, 1973 Stavale, Mariela El siguiente artículo se desprende de una investigación en curso y se centra en el análisis comparado de dos revistas político-culturales expresivas del peronismo revolucionario: Militancia Peronista para la Liberación y Envido. Ambas estuvieron atravesadas por las discusiones entre “movimientistas y alternativistas” dentro de la izquierda peronista y este artículo propone un análisis situado de ese debate, a partir de la comparación de dos experiencias coetáneas que expresaron ambas posiciones. Intentando arrojar luz sobre este proceso, se busca reponer las formas en que las revistas articularon los conceptos de «peronismo» y «revolución» en la compleja coyuntura del tercer peronismo, prestando especial atención a la forma en que ambas significaron la experiencia del gobierno y la identidad peronista revolucionaria.; The following article is clear from research in progress and focuses on the comparative analysis of two expressive politico-cultural magazines of the revolutionary peronism: Militant Peronist for the Liberation and Envido. Both were by discussions between “movimientistas and alternativistas” within the Peronist Left and here is a located analysis of this discussion from the comparison of two contemporary experiences that expressed both positions. Trying to shed light on this process, seeks to replace the forms in which the magazines articulated the concepts of “Peronism” and «revolution» in the complex situation of third peronism, paying special attention to the way in which both meant the experience of the Government and the revolutionary peronist identity.

La nación, la escuela y “los otros”: reflexiones sobre la historia de la educación en Argentina y Colombia en el imaginario civilizatorio moderno; The nation, the school and “the others”: Thoughts about the history of education in Argentina and Colombia in the modern civilizational imaginary; A nação, a escola e “os outros”: Reflexões sobre a história da educação na Argentina e na Colômbia no imaginário civilizatório moderno Ocoró Loango, Anny El presente artículo dirige la mirada hacia la historia de la educación en dos países latinoamericanos: Argentina y Colombia para decodificar cómo, desde sus orígenes como nación, la intención de afianzar la nacionalidad y la ciudadanía se usó para la exclusión, la invisibilización o la integración subordinada de las poblaciones y culturas que no eran consideradas parte constitutiva de la nación. También señala las limitaciones que han tenido los análisis desde el campo de la historia de la educación en América Latina para dar cuenta y problematizar lo suficiente el correlato racista y excluyente hacia los pueblos indígenas y las comunidades negras y afrodescendientes. Finalmente ofrece una lectura sobre las líneas de investigación y aportes que diferentes autores viene haciendo para analizar esta problemática desde los textos escolares.; This paper focuses on the history of education in two Latin American countries, Argentina and Colombia, aiming to decipher how, from their very origins as nations, the intention to strengthen nationality and citizenship was used to exclude, erase or subordinate the integration of populations and cultures that were not considered part of the nation. It also points out the limitations to the analysis from the history of education in Latin America to account for and sufficiently problematize the racist and exclusionary correlative with indigenous people and the black and African descendant communities. Finally, the paper offers a reading on the lines of research and contributions of different authors to analyze this problem based on the textbooks.; O presente artigo centra-se na história da educação em dois países latino-americanos: a Argentina e a Colômbia, para decodificar como, desde suas origens como nação, a intenção de consolidar a nacionalidade e a cidadania foi utilizada para a exclusão, a invisibilização ou a integração subordinada das populações e culturas que não eram consideradas parte da nação. Além disso, são assinaladas as limitações nas análises desde o campo da história da educação na América Latina, para dar conta e problematizar o discurso racista e excludente com os povos indígenas e as comunidades negras e afrodescendentes. Finalmente, oferece-se uma leitura sobre as linhas de pesquisa e contribuições que diversos autores fizeram para analisar esta problemática desde os textos escolares.

Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology Reinoso, Elina Beatríz; Bettera, Susana Gertrudis In this article, we describe a basic practical laboratory designed for fifth-year undergraduate students of Microbiology as part of the Epidemiology course. This practice provides the students with the tools for molecular epidemiological analysis of pathogenic microorganisms using a rapid and simple PCR technique. The aim of this work was to assay RAPD-PCR technique in order to infer possible epidemiological relationships. The activity gives students an appreciation of the value of applying a simple molecular biological method as RAPD-PCR to a discipline-specific question. It comprises a three-session laboratory module to genetically assay DNAs from strains isolated from a food outbreak.

Gamow states as solutions of a modified Lippmann-Schwinger equation Civitarese, Enrique Osvaldo; Gadella Urquiza, Manuel In this paper, we discuss the structure of Gamow states as solutions of Lippmann-Schwinger equation. The Friedrichs model is used to demonstrate it, both analytically and by applying perturbation theory to the extended spectrum of the Hamiltonian. The method presented here may be relevant to the inclusion of resonances in discrete basis, without the need of numerical constructions to define Gamow states, as entities depending on the choice of integrals contours, or as states resulting from ad hoc discretizations of the continuum.

Draft Genome Sequence of Photorhabdus luminescens Strain DSPV002N Isolated from Santa Fe, Argentina Palma, Leopoldo; del Valle, Eleodoro Eduardo; Frizzo, Laureano Sebastian; Berry, Colin; Caballero, Primitivo Here, we report the draft genome sequence of Photorhabdus luminescens strain DSPV002N, which consists of 177 contig sequences accounting for 5,518,143 bp, with a GC content of 42.3% and 4,701 predicted protein-coding genes (CDSs). From these, 27 CDSs exhibited significant similarity with insecticidal toxin proteins from Photorhabdus luminescens subsp. laumondii TT01.

Las mujeres instruidas asumen el desafío de tomar la palabra: representaciones de la Independencia en el fin de siglo Vignoli, Marcela El presente trabajo explora las distintas formas que asumió la participación femenina en festejos patrios que desde la celebración evocaban la Independencia de las Provincias Unidas de Sudamérica. Su desempeño para estas fechas pautadas por la liturgia patriótica como oradoras o escritoras de pequeños ensayos en las revistas literarias, así como desde la pintura y la música constituyen instantes visibles de un complejo proceso a través del cual se modificarían ciertos patrones de sociabilidad de índole cultural en Tucumán.

Germination response of Salsola schweinfurthii (Chenopodiaceae) to salinity and winged perianth removal Bhatt, A.; Pérez García, F.; Carón, María Mercedes; Gallacher, D. Salsola schweinfurthii is a perennial branched halophytic shrub that inhabits arid environments in and around the Arabian Peninsula. Its tolerance to extreme drought renders it suitable for urban arid landscaping. Germinability of intact and de-winged seeds (winged perianth removed) was determined under two photoperiods (0 and 12 hours light per day), three thermoperiods (daily low/high of 15/25, 20/30 and 25/35°C) and five salinity levels (0, 100, 200, 400 and 600 mM NaCl). Germination was maximised (93%) by de-winging and incubation in 12 hours light at 25/35°C. Intact and de-winged seeds both exhibited positive photoblastism. Germination of intact seeds was entirely prevented by the lowest level of salinity, and only slightly less prevented in de-winged seeds. Ability to germinate returned after saline solution was replaced with distilled water. The ability for S. schweinfurthii seeds to remain viable through a temporary period of salinity indicates an adaptation to unpredictable soil surface conditions in arid environments. Artificial propagation rates might be increased by removing perianths and sowing before or after mid-winter.

The herbicide glyphosate is a weak inhibitor of acetylcholinesterase in rats Larsen, Karen Elizabeth; Lifschitz, Adrian Luis; Lanusse, Carlos Edmundo; Virkel, Guillermo Leon The current work evaluated the inhibitory potency of the herbicide glyphosate (GLP) on acetylcholinesterase (AChE) activity in male and female rat tissues. The AChE activity in brain was higher (p < 0.05) than those observed in kidney (females: 2.2-fold; males: 1.9-fold), liver (females: 6-fold; males: 6.9-fold) and plasma (females: 14.7-fold; males: 25.3-fold). Enzyme activities were higher in presence of 10 mM GLP compared to those measured at an equimolar concentration of the potent AChE inhibitor dichlorvos (DDVP). Moreover, IC50s for GLP resulted between 6 × 104- and 6.8 × 105-fold higher than those observed for DDVP. In conclusion, GLP is a weak inhibitor of AChE in rats.

La obra testimonial de Pascual Coña: Arte verbal, documentación lingüística y cultural, luchas metadiscursivas; Pascual Coña’s testimonial work: Verbal art, linguistic and cultural documentation, metadiscursive struggles Golluscio, Lucia Angela Texto cultural construido sobre la base de acentos sociales profundamente en pugna, la obra testimonial de Pascual Coña ha interpelado a distintas generaciones de lectores-destinatarios y lo sigue haciendo, requiriendo de cada uno de ellos —y de nosotros— una toma de posición, una interpretación que estará siempre históricamente situada. A la luz de otras lecturas, este artículo examina los distintos posicionamientos del protagonista en tres contextos diferentes, aunque relacionados: la situación de producción discursiva, los nütram ‘historias’ narrados por Coña y el circuito de la obra publicada. Desde una perspectiva que reconoce en esta obra la interacción de múltiples voces, el análisis incorpora la noción de zona de contacto, entendida como «el espacio social en que pueblos geográfica e históricamente separados entran en contacto y establecen relaciones duraderas basadas en la coerción, la inequidad y el conflicto» (Pratt, 1997). Se concluye que la obra de Coña, por un lado, logra subvertir desde el «no poder» el rol de autor que Moesbach se (auto)adjudica en la primera edición y, por otro, cobra su sentido más profundo en el marco de la saga de discursos circulantes en el área sobre los destinos trágicos sufridos por destacados longko mapuches a ambos lados de los Andes, luego de la derrota.; As a cultural text based on deeply conflicted social accents, Pascual Coña’s testimonial work continues to question different generations of readers-addresses, asking each of them —and us— to take a position, to make an interpretation of the events and the participants which would always be historically located. In the light of other previous and contemporary readings, this article examines Coña’s diverse and even opposite footings across time in three dissimilar though related contexts: discourse production, the nütram ‘stories’ included in the book, and the circulation contexts for the published work. From a perspective that distinguishes in this work the interaction of multiple voices, the analysis incorporates the notion of contact zone, understood as the social space in which geographically and historically separated peoples come into contact and establish lasting relations based on coercion, inequality, and conflict (Pratt, 1992). It concludes that Pascual Coña’s work, on the one hand, manages to subvert the «author» role that Moesbach allocated to himself in the first edition and, on the other, it also gains profound sense within the framework of the saga of narratives circulating in the area about the tragic fates of leading Mapuche longko on both sides of the Andes after their defeat.

Characterization of the interaction between the HIV-1 Gag structural polyprotein and the cellular ribosomal protein L7 and its implication in viral nucleic acid remodeling El Mekdad, Hala; Boutant, Emmanuel; Karnib, Hassan; Biedma, Marina Elizabeth; Sharma, Kamal Kant; Malytska, Iuliia; Laumond, Géraldine; Roy, Marion; Réal, Eléonore; Paillart, Jean Christophe; Moog, Christiane; Darlix, Jean Luc; Mély, Yves; de Rocquigny, Hugues Background: In HIV-1 infected cells, the integrated viral DNA is transcribed by the host cell machinery to generate the full length HIV-1 RNA (FL RNA) that serves as mRNA encoding for the Gag and GagPol precursors. Virion formation is orchestrated by Gag, and the current view is that a specific interaction between newly made Gag molecules and FL RNA initiates the process. This in turn would cause FL RNA dimerization by the NC domain of Gag (GagNC). However the RNA chaperoning activity of unprocessed Gag is low as compared to the mature NC protein. This prompted us to search for GagNC co-factors. Results: Here we report that RPL7, a major ribosomal protein involved in translation regulation, is a partner of Gag via its interaction with the NC domain. This interaction is mediated by the NC zinc fingers and the N- and C-termini of RPL7, respectively, but seems independent of RNA binding, Gag oligomerization and its interaction with the plasma membrane. Interestingly, RPL7 is shown for the first time to exhibit a potent DNA/RNA chaperone activity higher than that of Gag. In addition, Gag and RPL7 can function in concert to drive rapid nucleic acid hybridization. Conclusions: Our results show that GagNC interacts with the ribosomal protein RPL7 endowed with nucleic acid chaperone activity, favoring the notion that RPL7 could be a Gag helper chaperoning factor possibly contributing to the start of Gag assembly.

Las trayectorias furtivas: tránsitos posibles de la escolarización; Furtive paths: Transits possible schooling Martínez, Darío Gabriel Las preocupaciones por las trayectorias educativas de hombres y mujeres de la educación de jóvenes y adultos pueden otorgar lineamientos acerca de la especificidad de estos ámbitos, como también de los sentidos que le asigna lo educativo en el interior de una dinámica institucional. Recorrer este plano de análisis brindará aproximaciones en torno de los sentidos asignados a la escolarización por parte de aquellos sectores sociales que fueron desplazados —por diversos motivos— de un contacto estable con determinadas instituciones educativas. Por ello, aquí se examinarán las características más recurrentes de las trayectorias educativas relevadas, en una articulación con aquellos rasgos biográficos que hilvanó la relación establecida con las instituciones. Este es un modo de interrogarse que favorecerá inquietudes sobre los motivos de un determinado modo de transitar un camino posible de la escolarización. También se analizarán las cuestiones que alumnos y alumnas identifican como los atributos específicos que diferencian al ámbito educativo al que concurren de aquel que asistían en otro momento de sus vidas.; Being concerned about the educational paths of youngs, adults, men or women may provide us with guidelines about the specificity of these areas. In addition, we can find which senses are made about these processes that are assigned to the interior of the institutional dynamics. This level of analysis will provide us an approach to the senses assigned to the schooling process by those social sectors that were displaced — for different reasons— from being in regular contact with certain educational institutions. Therefore, the most frequent characteristics of educational trajectories will be analyzed in here, in connection with the biographical traits that were surveyed and the established relationship with the institutions. In this is way, we can find different concerns that people have about passing through a possible schooling process. Similarly, distinguishing features that differentiate their current learning environment from the ones they experienced some time ago will be also analyzed.

Platelets: New bricks in the building of neutrophil extracellular traps Carestia, Agostina; Kaufman, Tomás; Schattner, Mirta Ana In addition to being key elements in hemostasis and thrombosis, platelets have an important role in the inflammatory and innate immune response. This activity is associated with their capability to recognize pathogens through the expression of toll-like receptors, the secretion of various cytokines, chemokines, and growth factors stored within their granules, and the expression of cell adhesion molecules that allows interaction with other immune cells, mainly neutrophils and monocytes. As part of the first line of defense, neutrophils control invading pathogens by phagocytosis, the release of antimicrobial proteins during degranulation, or through the formation of web-like structures named neutrophil extracellular traps (NETs). NETs are formed by chromatin, proteases, and antimicrobial proteins, and their main function is to trap and kill bacteria, virus, and fungi, avoiding their dissemination. Besides microorganisms, NET formation is also triggered by proinflammatory molecules and platelets. The uncontrolled formation of NETs might exert tissue damage and has been involved in a pathogenic mechanism of autoimmune and prothrombotic clinical conditions. In this review, we discuss the role of platelets in NET generation highlighting the mediators, stimuli, and molecular mechanisms involved in this phenomenon, both in human and murine models.

Enhancement by GOSPEL protein of GAPDH aggregation induced by nitric oxide donor and its inhibition by NAD+ Gonzalez, María C.; Romero, Jorge Miguel; Ingaramo, María Clara; Muñoz Sosa, Christian Javier; Curtino, Juan Agustin; Carrizo Garcia, Maria Elena Glyceraldehyde-3-phosphate dehydrogenase's (GAPDH's) competitor of Siah Protein Enhances Life (GOSPEL) is the protein that competes with Siah1 for binding to GAPDH under NO-induced stress conditions preventing Siah1-bound GAPDH nuclear translocation and subsequent apoptosis. Under these conditions, GAPDH may also form amyloid-like aggregates proposed to be involved in cell death. Here, we report the in vitro enhancement by GOSPEL of NO-induced GAPDH aggregation resulting in the formation GOSPEL-GAPDH co-aggregates with some amyloid-like properties. Our findings suggest a new function for GOSPEL, contrasting with its helpful role against the apoptotic nuclear translocation of GAPDH. NAD+ inhibited both GAPDH aggregation and co-aggregation with GOSPEL, a hitherto undescribed effect of the coenzyme against the consequences of oxidative stress.

Production in stirred-tank bioreactor of recombinant bovine chymosin B by a high-level expression transformant clone of Pichia pastoris Noseda, Diego Gabriel; Recúpero, Matías Nicolás; Blasco, Martín; Bozzo, Joaquín Ignacio; Galvagno, Miguel Angel An intense screening of Pichia pastoris clones transformed with the gene of bovine chymosin under methanol-inducible AOX1 promoter was performed, obtaining a transformant clone with a higher milk-clotting activity value in comparison with our previous studies. The scaling of recombinant-chymosin production was carried out by a fed-batch strategy in a stirred-tank bioreactor using biodiesel-byproduct crude glycerol as the carbon source and pure methanol for the induction of chymosin expression, achieving a biomass concentration of 158 g DCW/L and a maximum coagulant activity of 192 IMCU/ml after 120 h of methanol induction. Recombinant bovine chymosin was purified from bioreactor-fermentation culture by a procedure including anion-exchange chromatography which allowed obtaining heterologous chymosin with high level of purity and activity; suggesting that this downstream step could be scaled up in a successful manner for chymosin purification. Thermoestability assay permitted to establish that unformulated recombinant chymosin could be stored at 5 °C without decrease of enzyme activity throughout at least 120 days. Finally, reiterative methanol-inductions of recombinant chymosin expression in bioreactor demonstrated that the reutilization of cell biomass overcame the low enzyme productivity usually reached by P. pastoris system.

Agregados reciclados pretratados para uso en hormigón; Pretreatment recycled aggregates for concrete Priano, Carla; Señas, Lilia Norma; Moro, Juan Manuel; Marfil, Silvina Andrea Los residuos de construcción, principalmente aquellos que provienen de la trituración de hormigones de demolición, pueden ser utilizados como reemplazo total o parcial del agregado natural. Como consecuencia de mayor porosidad, el agregado reciclado resulta menos denso, y con una mayor capacidad de absorción de agua que el natural.En este trabajo se presentan distintos tratamientos de impregnación sobre el agregado grueso reciclado de un hormigón de canto rodado, con el fin de mejorar la calidad del hormigón resultante y optimizar su uso. Se analizaron tres tipos de productos para tratar al agregado reciclado (dos comerciales de uso frecuente en el mercado y una pasta cementícea), y diferente tiempo entre la ejecución del tratamiento y su incorporación al hormigón. Se estudiaron propiedades mecánicas y físicas de hormigones con un reemplazo del 50% del agregado grueso natural por hormigón triturado. Se realizó un estudio petrográfico sobre las diferentes muestras a fin de determinar la influencia de los tratamientos en la morfología de la zona de interfase agregado-mortero.El tratamiento previo de impregnación del agregado reciclado demostró ser una alternativa para mejorar la estructura de poros del hormigón y llevarlo a un comportamiento similar al de un hormigón convencional. El uso de un material de desecho permite reducir la cantidad de residuos generados una vez cumplida la vida útil de las estructuras y disminuir la explotación de recursos naturales no renovables como son los agregados para hormigón.; Construction waste, mainly those coming from crushed demolition concrete, can be used as partial or complete replacement of natural aggregates. Due to their greater porosity, recycled aggregates have lower density and higher water absorption than natural ones.This research presents different impregnation treatments of the recycled coarse aggregate obtained from concrete containing boulder, in order to improve final concrete quality and optimize their use. Three types of products were analyzed to treat the recycled aggregate (two highly used commercial products and one cement paste), as well as different time between treatment execution and its addition to the concrete. Mechanical and physical properties of concretes with 50% of natural coarse aggregate replacement by crushed concrete were studied. A petrographic study of the different samples was performed to determine the treatment influence on the morphology of the aggregate-mortar interface zone. Previous impregnation treatment of the recycled aggregate proved to be an alternative to improve concrete pore structure and to allow a similar behavior to conventional concretes. The use of waste materials reduces the amount of residues generated once the useful life of the structure ends and decreases the exploitation of non-renewable natural resources like concrete aggregates.