Argentina 1983/1985. Expectativas del momento inaugural Lesgart, Cecilia El artìculo reconstruye como van transformàndose, en el tiempo corto de la polìtica, los sentidos de la democracia con la que se produce la "transiciòn" en Argentina durante 1983. Asimismo, va marcando los principales acontecimientos que le dan sentido a la misma entre 1982 y 1987.

Eocene paleoceanographic and paleoclimatic events recognized by assemblages of dinoflagellate cysts in the Southwest Atlantic Ocean Premaor, Eduardo; Ferreira, Elizabete P.; Guerstein, Gladys Raquel; Alves de Souza, Paulo Variations in the relative frequencies between cosmopolitan dinoflagellate cysts and Antarctic assemblage species indicate temperature changes and variations of ocean currents during the Paleogene. This study is based on the analysis of 23 samples from two stratigraphic intervals from the Well BP-01 (Imbé Formation), drilled by in the Pelotas Basin offshore. We identified 66 dinoflagellate cyst taxa, 47 of them belong to the Order Gonyaulacales and 19 to the Order Peridiniales. The relative abundance data highlight two different dinoflagellate ecogroups represented by Apectodinium and Enneadocysta in the intervals A and B, respectively. The level with the highest percentages of Apectodinium recorded in the Calcareous Nannofossil Zone N-410 (Ypresian), suggest that the Apectodinium maximum represents the Early Eocene Maximum Thermal Event (EMT-2). Enneadocysta Acmes registered near the Bartonian/Priabonian boundary, can be correlated with a drop in sea surface temperature that occurred after the Mid-Eocene Climatic Optimum. Dinoflagellate occurrences suggest deposition in open sea conditions during the transgressive phase. The P/G ratio does not reach significant values in the analyzed intervals, indicating low dissolved nutrient concentrations.

Factores lógicos y extra-lógicos en fallos de la Corte Suprema Argentina; Logical and extra-logical factors in rulings of the Argentine Supreme Court Macbeth, Guillermo Eduardo El propósito de este estudio es detectar la presencia de razonamientos deónticos y argumentaciones dialógicas en sentencias de la Corte Suprema de Justicia Argentina. Para ello, se realizó un estudio descriptivo que permitió registrar y comparar factores lógicos y extra-lógicos en 157 años de sentencias del máximo tribunal argentino. Se empleó un algoritmo que analizó todas las sentencias publicadas por la Corte entre 1865 y 2022. Se construyeron índices específicos de marcadores lingüísticos. La evidencia mostró que la mencionada Corte razona y argumenta, en coherencia con teorías centradas en factores lógicos, por un lado, y en factores extra-lógicos, por otro lado. Se deduce la necesidad de una nueva teoría psicológica que integre ambos factores en un mismo modelo explicativo.; The aim of this study is to detect the presence of deontic reasoning and dialogic arguments in rulings of the Argentine Supreme Court of Justice. To this end, a descriptive study was conducted to detect and compare logical and extra-logical factors in 157 years of rulings published by the highest Argentine court. An algorithm was applied to analyze all the rulings published by the Court between 1865 and 2022. Specific groups of linguistic markers were constructed. The evidence showed that the aforementioned Court reasons and argues, in coherence with theories focused on logical factors, on the one hand, and on extra-logical factors, on the other hand. The need for a new psychological theory that integrates both factors in the same explanatory model is deduced.

General relativistic pulsations of ultra-massive ZZ Ceti stars Corsico, Alejandro Hugo; Boston, S Reece; Althaus, Leandro Gabriel; Kilic, Mukremin; Kepler, S. O.; Camisassa, María Eugenia; Torres, Santiago Ultra-massive white dwarf stars are currently being discovered at a considerable rate, thanks to surveys such as the Gaia space mission. These dense and compact stellar remnants likely play a major role in type Ia supernova explosions. It is possible to probe the interiors of ultra-massive white dwarfs through asteroseismology. In the case of the most massive white dwarfs, General Relativity could affect their structure and pulsations substantially. In this work, we present results of relativistic pulsation calculations employing relativistic ultra-massive ONe-core white dwarf models with hydrogen-rich atmospheres and masses ranging from 1.29 to 1.369M⊙ with the aim of assessing the impact of General Relativity on the adiabatic gravity (g)-mode period spectrum of very-high mass ZZ Ceti stars. Employing the relativistic Cowling approximation for the pulsation analysis, we find that the critical buoyancy (Brunt-Väisälä) and acoustic (Lamb) frequencies are larger for the relativistic case, compared to the Newtonian case, due to the relativistic white dwarf models having smaller radii and higher gravities for a fixed stellar mass. In addition, the g-mode periods are shorter in the relativistic case than in the Newtonian computations, with relative differences of up to ~50 % for the highest-mass models (1.369M⊙) and for effective temperatures typical of the ZZ Ceti instability strip. Hence, the effects of General Relativity on the structure, evolution, and pulsations of white dwarfs with masses larger than ~1.29M⊙ cannot be ignored in the asteroseismological analysis of ultra-massive ZZ Ceti stars.

An early treatment with BKI-1748 exhibits full protection against abortion and congenital infection in sheep experimentally infected with Toxoplasma gondii Sánchez Sánchez, Roberto; Imhof, Dennis; Hecker, Yanina Paola; Ferre, Ignacio; Re, Michela; Moreno Gonzalo, Javier; Blanco Murcia, Javier; Mejías López, Elena; Hulverson, Matthew; Choi, Ryan; Arnold, Samuel; Ojo, Kayode; Barrett, Lynn; Hemphill, Andrew; Van Voorhis, Wesley; Ortega Mora, Luis Miguel Congenital toxoplasmosis in humans and in some mammalian species, such as small ruminants, is a well-known cause of abortion and foetal malformations. The calcium-dependent protein kinase 1 (CDPK1) inhibitor BKI-1748 has shown a promising safety profile for its use in humans and a good efficacy against Toxoplasma gondii infection in vitro and in mouse models. The rates of congenital infection and foetal damage in sheep seem to mimic the situation in human toxoplasmosis more closely than those in mouse models. Ten doses of BKI-1748 given every other day orally in sheep at 15 mg/kg exhibited therapeutic plasma levels for 23 days and no systemic or pregnancy-related toxicity was observed. In sheep experimentally infected at 90 days of pregnancy with a T. gondii oocyst dose which was lethal for all foetuses, the BKI-1748 treatment administered from 48 hours after infection led to complete protection against abortion and congenital infection. In addition, compared to infected/untreated sheep, treated sheep showed a drastically lower rectal temperature increase, higher IFNγ levels and none showed IgG seroconversion throughout the study. In conclusion, BKI-1748 treatment in pregnant sheep starting at 48 hours after infection was fully effective against congenital toxoplasmosis.

Pajaritos de papel: Análisis didáctico de una clase de formación vocal con aportes de la perspectiva semiótica; Paper birds: Didactic analysis or a vocal traning class with contributions from semiotic perspective Angelelli, Maria Belen; Martini, Ana Carolina; Carrera, Mariela El trabajo expone el análisis didáctico (Steiman, 2010) de una clase de Educación Vocal en la Universidad Provincial de Córdoba (UPC), Argentina. Desde una perspectiva transdisciplinaria y experiencial, identificamos núcleos problemáticos que giran en torno al espacio, el tiempo y los sujetos que intervienen en el proceso pedagógico de la clase, y en las decisiones y los supuestos que la docente llevó a cabo. Es decir, en aquellos componentes que hacen a las “configuraciones estéticas” de la clase (Roldán, 2022). En cuanto al aspecto metodológico, retomamos los registros de observación de la clase y los intercambios que tuvimos con la docente, también autora de este trabajo. Nos basamos en el guion conjetural que previamente había realizado y en su proyecto de cátedra. Desde las fronteras disciplinarias, entre la comunicación, la semiótica y la didáctica, planteamos nuestro análisis sobre el cronotopo áulico, un espacio-tiempo donde el celular es protagonista, aunque nuestros ojos no lo vean, atravesado por una cultura de la conectividad (Van Dijk, 2016).; This paper presents the didactic analysis (Steiman, 2010) of a Vocal Education class at the UPC. From a transdisciplinary perspective, located in the disciplinary and experiential boundaries in which we place ourselves as authors, we identify problematic nuclei that revolve around: space-time-subjects involved in the pedagogical process of the class, and in the decisions and assumptions that the teacher in charge carried out. That is, in those components that make up the "aesthetic configurations" of the class (Roldán, 2022). Methodologically, we took up again the observation records of the class and the exchanges we had with the teacher, also the author of this work, and we were guided by the conjectural script she had previously made and by her teaching project. From the disciplinary boundaries, between communication, semiotics and didactics, we propose our analysis of the classroom chronotope, a space-time where the cell phone is the protagonist although our eyes do not see it, also crossed by a culture of connectivity (Van Dijk, 2016).

“Turismo de trasplantes” e (in)securitización de las movilidades por salud; “Transplant Tourism” and (In)securitization of Health Mobilities Basualdo, María Lourdes El objetivo de este artículo es analizar el proceso de (in)securitización de las movilidades por salud definidas como “turismo de trasplante”. Específicamente indaga el surgimiento y circulación de la categoría “turismo de trasplantes”, así como ciertos usos políticos del concepto en regulaciones sobre trasplante y migración en Argentina. Sostiene que el “turismo de trasplantes” fue construido como un problema de (in)seguridad asociado a los peligros y amenazas que determinadas movilidades representan para el “orden nacional” de los trasplantes y para la “seguridad internacional” a partir de su asociación con modalidades ilícitas de extracción y comercialización de órganos. El artículo adopta una metodología cualitativa que articula análisis de diversas fuentes documentales y entrevistas con funcionarias/os de gobierno del contexto argentino.; This article seeks to analyze the process of in(in)securitization of health mobilities defined as "transplant tourism.” It focusses on the emergence and circulation of the "transplant tourism" category, as well as certain political uses of the concept in transplant and migration regulations in Argentina. It argues that "transplant tourism" was constructed as a problem of (in)security associated with the dangers and threats posed by certain types of mobilities to the "national order" of transplants and “international security,” because of its association with illicit forms of organ extraction and commercialization. The article uses a qualitative methodology combining the analysis of documentary sources with interviews with government officials in the Argentinean context.

Segmentación socioeconómica a usuarios finales de la energía eléctrica: Política tarifaria de Salta durante 2022; Socio-economic segmentation of electricity end-users: Salta's tariff policy during 2022 Hessling Herrera, Franco David Los servicios de energía son considerados básicos para hogares desde el punto de vista del derecho humano a la vivienda adecuada (art. 25 de la Declaración Universal de los Derechos Humanos). A principios de 2022, Argentina anunció un acuerdo de refinanciación de deuda con el FMI, en el que se establecía la quita de subsidios a esos servicios. A principios de abril, la Subsecretaría de Planeamiento Energético de la Nación emitió un informe técnico anticipando una “segmentación tarifaria”, y dos semanas después, Salta fue la primera jurisdicción en aplicar dicho criterio para la regulación de las facturas emitidas por la distribuidora provincial. En este artículo se caracteriza el Mercado Eléctrico Mayorista, se contextualiza la política tarifaria y se analiza específicamente la implementación de la segmentación en Salta. Para ello, se analizan documentos públicos y entrevistas realizadas para esta investigación con actores claves. Por último, se presentan algunas consideraciones sobre esa implementación y los efectos que podría tener en el mediano y largo plazo.; Energy services are considered basic for homes from the point of view of the human right to adequate housing (art. 25 of the Universal Declaration of Human Rights). At the beginning of 2022, Argentina announced a debt refinancing agreement with the IMF, which established the removal of subsidies for these services. At the beginning of April, the Subsecretary of Energy Planning of the Nation issued a technical report anticipating a "tariff segmentation", and two weeks later, Salta was the first jurisdiction to apply that criteria to regulate the invoices issued by the provincial distributor. This article characterizes the Wholesale Electricity Market, contextualizes the tariff policy and specifically analyzes the implementation of segmentation in Salta. To do this, public documents and interviews conducted for this research with key actors are analyzed. Finally, some considerations on this implementation and the effects it could have in the medium and long term are presented.

Determination of crystallographic texture in polycrystalline materials from wavelength-resolved neutron transmission experiments: application to high-symmetry crystals Vicente Alvarez, Miguel Angel; Malamud, Florencia; Santisteban, Javier Roberto Wavelength-resolved neutron transmission experiments are useful for characterizing the microstructure of macroscopic specimens with 2D spatial resolution perpendicular to the beam direction. The crystallographic texture can affect the neutron transmission in the thermal neutron energy range, which manifests as changes in the shape and height of Bragg edges as a function of neutron wavelength. Models have been proposed to predict the transmission of textured polycrystalline materials from knowledge of the material texture and have proved to accurately predict the observed transmission data. In recent work, a novel method was described and tested for obtaining texture integral parameters from the combined analysis of transmission data measured along several directions of a specimen in a hexagonal crystal Zr alloy. However, this procedure has limitations when dealing with high-symmetry crystal structures. In this work, a generalization of such a method based on the expansion of the orientation distribution function (ODF) in symmetric generalized spherical harmonics that is applicable to all crystal and sample symmetries is presented. Using this method, the low-order Fourier coefficients of the ODF can be estimated by analyzing transmission data obtained for a reduced set of beam directions. This method was verified using a cubic Cu sample, for which transmission data were available along five different directions. Two sample symmetries were assumed to reduce the number of Fourier coefficients of the ODF. In the case of cylindrical symmetry (fiber-type texture), the results were good; but in the case of orthorhombic symmetry, some bias was observed which was attributed to the reduced number of beam directions used to perform the evaluation.

Efecto de la intensidad de las lluvias sobre la acumulación de resina de Grindelia chiloensis en el suelo: implicancias para la restauración ecológica Rajnoch, María Gimena; Pérez, D. R.; Ravetta, Damián Andrés En ambientes áridos, debido a las presiones ambientales, las plantas han desarrollado una gran diversidad de metabolitos secundarios carbonados como las resinas, los politerpenos, las ceras, las gomas, entre otros. En particular, para las resinas se han encontrado varias funciones y se ha evidenciado la formación de costras fitoquímicas en el suelo por su acumulación bajo el canopeo de plantas como Grindelia chiloensis (Asteraceae) y Larrea divaricata (Zygophilaceae). Estas resinas modifican las propiedades físicas del suelo y la dinámica del agua en el suelo. En este trabajo caracterizamos el transporte de ácidos diterpénicos de G. chiloensis, generado por las lluvias, hacia la superficie del suelo y su acumulación. A partir de plántulas fue posible promover la formación de costras fitoquímicas en un período de dos años, en un experimento en el que se simularon precipitaciones de diferentes intensidades. Nuestros resultados también muestran que la intensidad de las precipitaciones determina la cantidad de resina removida y el contenido de resina del suelo. Debido a su efecto en las propiedades físicas del suelo y en la dinámica del agua, se propone que las costras fitoquímicas podrían modular procesos biológicos del suelo y tener una aplicación potencial para reiniciar la sucesión ecológica de sitios degradados o desertificados, al igual que las costras biológicas. En este contexto, los resultados obtenidos nos permiten pensar en diseños para la restauración que promuevan, imiten o aceleren el proceso natural de formación de costras.

El Genocidio como un dispositivo del olvido, la literatura como un dispositivo de la memoria: la Shoá y la Dictadura en la literatura argentina; Genocide as a tool to forget, literature as a tool to remember: the Shoah and the Dictatorship in Argentine literature Niemetz, Diego En la órbita de la postmemoria, heredar la experiencia de los mayores es un tópico revisitado una y otra vez por los escritores argentinos contemporáneos. Este trabajo se centra en el análisis de cuatro obras de la literatura argentina reciente que recuperan los relatos de los antepasados en forma de memoria familiar, pero que además proponen una reflexión acerca de la transmisibilidad de esas memorias y de la carga que supone. A partir de la superposición de experiencias familiares durante diferentes procesos genocidas, los textos postulan un borramiento de fronteras geográficas y temporales, que repercute en la presentación de los hechos y que, a partir de ese mecanismo, perduran en un presente sin solución de continuidad con el pasado ni con el futuro. Entre la persistencia y la superposición de las memorias genocidas y el trauma entendido como herencia, las producciones analizadas parecen perseguir un fin emancipatorio, de acuerdo a una de las famosas tesis sobre la historia de Walter Benjamin.; In the orbit of postmemory, inheriting the experience of the elders is a topic revisited over and over by contemporary Argentine writers. This article focuses on the analysis of four works of recent Argentine literature that recover the stories of ancestors in the form of family memories, but also propose a reflection on the transmissibility of these memories and the burden that they place on a person. Based on the overlapping of family experiences during different genocidal processes, the texts postulate an erasure of geographical and temporal borders, which affects the presentation of the facts and which, based on this mechanism, persist in a present that melts with the past or the future. Between the persistence and overlapping of genocidal memories and trauma understood as inheritance, the analyzed productions seem to pursue an emancipatory goal, according to one of Walter Benjamin’s famous theses on history.

Entre la opacidad y la transparencia: Cine, transición y monstruos; Between opacity and transparency: Cinema, transition and monsters Montes, Viviana Andrea Los estudios sobre el cine argentino de la primera década posdictadura se han planteado con frecuencia en términos de polarización: opacidad o transparencia, continuidad o ruptura con el cine previo. Otra clave de lectura interesante para abordar la producción audiovisual del decenio puede ser su eminente carácter transicional. El cine de esos años, por un lado, fue testigo y artífice de la transición política entre terrorismo de Estado y democracia; por otro, se vio conmovido por la transición tecnológica que impuso el avance del video como nuevo modo de producción y de circulación de imágenes. Al mismo tiempo vivenció su propia transición, que denominaremos transición cinematográfica.La transición se presenta como intersticio entre lo viejo y lo nuevo que se entrelazan creando formas que comparten características del tiempo pretérito y del porvenir sin identificarse completamente con ninguno de ellos. La coexistencia –no sin disputa- de narrativas y modos de representación es un rasgo tan frecuente como distintivo. Este trabajo busca indagar en los monstruos que habitan el claroscuro de ese cine constituyéndolo como espacio de querella entre lo viejo y lo nuevo. Esos monstruos se ligan a la acuciante presencia del pasado reciente en la narrativa cinematográfica del cine de la primera década de la posdictadura argentina y se expresan en las temáticas de los filmes, en la construcción de los espacios o en los vínculos interpersonales que componen los primeros trazos de la(s) memoria(s) que desde el campo cultural comenzaban a escribirse.; Studies on Argentine cinema in the first post-dictatorship decade have often been approached in terms of polarization: opacity or transparency, continuity or rupture with previous cinema. Another interesting reading key to approach the audiovisual production of the decade may be its eminent transitional character. The cinema of those years, on the one hand, was witness and architect of the political transition between State terrorism and democracy; on the other hand, it was moved by the technological transition that imposed the advance of video as a new mode of production and circulation of images. At the same time, it experienced its own transition, which we will call cinematographic transition. The transition is presented as an interstice between the old and the new that intertwine to create forms that share characteristics of the past and the future without being completely identified with either of them. The coexistence -not without dispute- of narratives and modes of representation is a feature as frequent as it is distinctive. This paper seeks to investigate the monsters that inhabit the chiaroscuro of this cinema, constituting it as a space of conflict between the old and the new. These monsters are linked to the pressing presence of the recent past in the cinematic narrative of the first decade of the Argentine post-dictatorship and are expressed in the themes of the films, in the construction of the spaces or in the interpersonal links that make up the first traces of the memories that were beginning to be written from the cultural field.

Una teoría del Estado para la democracia argentina Nosetto, Luciano Ezequiel Más de una vez sostuvo Eduardo Rinesi que la Argentina de los años 80 dedicó los mayores esfuerzos de su imaginación política a pensar la transición a la democracia, soslayando sin embargo la reflexión sobre el Estado. Si bien los monográficos de Guillermo O’Donnell sobre el Estado burocrático autoritario y de Oscar Oszlak sobre la formación del Estado argentino se producen hacia fines del 70 y comienzos del 80, lo cierto es que, a poco de andar la nueva década, el desafío de transitar a la democracia termina atrayendo la mayoría de los esfuerzos intelectuales de la época. De allí que Cecilia Lesgart pueda afirmar que “la transición a la democracia sustituyó las reflexiones sobre el Estado latinoamericano”. Esta marca de origen explicaría en parte el hecho de que, 40 años después, contemos con robustas y sopesadas teorías de la democracia, pero que nos cueste todavía articular una teoría del Estado consistente.

Multiple challenges in the development of commercial crops using CRISPR/Cas technology Ayub, Nicolas Daniel; Soto, Gabriela Cynthia The CRISPR/Cas system is a highly efficient and versatile tool for editing plant genomes, with the potential to accelerate breeding programs and improve the sustainability of food production. Nevertheless, technical limitations delay the rapid spread of the CRISPR/Cas system benefits in agriculture. The natural features of plant species, including reproductive behavior, ploidy levels, genetic diversity, and generation times, can significantly impact the introgression of edited traits into elite germplasms. The production and selection of edited events require the same level of effort as those of their transgenic equivalents. Additionally, edited alleles tend to be recessive or not fully dominant, which differs from dominant transgenic events. To accelerate the introgression of edited events into conventional and transgenic varieties, we suggest utilizing edits on single-copy genes that induce dominant mutations. In the absence of new, simple traits that provide exceptional economic benefits for large companies, like herbicide tolerance in transgenic crops, we propose the emergence of particular public grants for edited variety productions, especially when the introgression shows a high level of technical feasibility. In the context of climate change, these public actions must be taken quickly to alleviate significant reductions in crop production.

Robust Molecular Anodes for Electrocatalytic Water Oxidation Based on Electropolymerized Molecular Cu Complexes Amthor, Sebastian; Ranu, Koushik; Bellido, Carlos G.; Salomón, Fernando Federico; Piccioni, Alberto; Mazzaro, Raffaello; Boscherini, Federico; Pasquini, Luca; Gil Sepulcre, Marcos; Llobet, Antoni A multistep synthesis of a new tetra-amidate macrocyclic ligand functionalized with alkyl-thiophene moieties, 15,15-bis(6-(thiophen-3-yl)hexyl)-8,13-dihydro-5H-dibenzo[b,h][1,4,7,10]tetraazacyclotridecine-6,7,14,16(15H,17H)-tetraone, H4L, is reported. The reaction of the deprotonated ligand, L4−, and Cu(II) generates the complex [LCu]2−, that can be further oxidized to Cu(III) with iodine to generate [LCu]−. The H4L ligand and their Cu complexes have been thoroughly characterized by analytic and spectroscopic techniques (including X-ray Absorption Spectroscopy, XAS). Under oxidative conditions, the thiophene group of [LCu]2− complex polymerizes on the surface of graphitic electrodes (glassy carbon disks (GC), glassy carbon plates (GCp), carbon nanotubes (CNT), or graphite felts (GF)) generating highly stable thin films. With CNTs deposited on a GC by drop casting, hybrid molecular materials labeled as GC/CNT@p-[LCu]2− are obtained. The latter are characterized by electrochemical techniques that show their capacity to electrocatalytically oxidize water to dioxygen at neutral pH. These new molecular anodes achieve current densities in the range of 0.4 mA cm−2 at 1.30 V versus NHE with an onset overpotential at ≈250 mV. Bulk electrolysis experiments show an excellent stability achieving TONs in the range of 7600 during 24 h with no apparent loss of catalytic activity and maintaining the molecular catalyst integrity, as evidenced by electrochemical techniques and XAS spectroscopy.

Melanoma cells with acquired resistance to vemurafenib have decreased autophagic flux and display enhanced ability to transfer resistance Perez, Celia Noemí; Falcón, Cristian Roberto; Delgado Mons, Johinna Ileana; Cuello Orlandi, Federico; Sangiacomo, Mercedes; Fernandez Muñoz, Juan Manuel; Guerrero, Martín; Benito Rodriguez, Paula Guadalupe; Colombo, Maria Isabel; Zoppino, Felipe Carlos Martin; Alvarez, Sergio Eduardo Over the last years, the incidence of melanoma, the deadliest form of skin cancer, has risen significantly. Nearly half of the melanoma patients exhibit the BRAFV600E mutation. Although the use of BRAF and MEK inhibitors (BRAFi and MEKi) showed an impressive success rate in melanoma patients, durability of response remains an issue because tumor quickly becomes resistant. Here, we generated and characterized Lu1205 and A375 melanoma cells resistant to vemurafenib (BRAFi). Resistant cells (Lu1205R and A375R) exhibit higher IC50 (5–6 fold increase) and phospho-ERK levels and 2–3 times reduced apoptosis than their sensitive parents (Lu1205S and A375S). Moreover, resistant cells are 2–3 times bigger, display a more elongated morphology and have a modulation of migration capacity. Interestingly, pharmacological inhibition of sphingosine kinases, that prevents sphingosine-1-phosphate production, reduces migration of Lu1205R cells by 50 %. In addition, although Lu1205R cells showed increased basal levels of the autophagy markers LC3II and p62, they have decreased autophagosome degradation and autophagy flux. Remarkably, expression of Rab27A and Rab27B, which are involved in the release of extracellular vesicles are dramatically augmented in resistant cells (i.e. 5–7 fold increase). Indeed, conditioned media obtained from Lu1205R cells increased the resistance to vemurafenib of sensitive cells. Hence, these results support that resistance to vemurafenib modulates migration and the autophagic flux and may be transferred to nearby sensitive melanoma cells by factors that are released to the extracellular milieu by resistant cells.

A Stochastic Detection Models Comparison in Turbulent Flow Events Calandra, Maria Valeria; Marañon Di Leo, Julio The need to establish the downwind fluid dynamic field of aerodynamic bodies subjected to a givenvelocity field is well known, to verify their aerodynamic characteristics. In this context, differenttechniques allow us to establish the characteristics of the field. It is almost always necessary to carry out quantitative determinations to describe the field correctly, particularly when the field is made up of turbulent wakes. In this sense, in the experimental field, it is common to use hot-wire anemometry techniques, which have great capabilities to quantify high-frequency events. Previous work has analyzed the determination of changes in hot-wire anemometry signals for the detection of events in turbulent flows with different models, based on stochastic algorithms (CPM - Change Point Model).The present work aims to compare the results obtained previously with the application of different CPM models developed. Previously applied and evaluated measurements are used, the implementation of the models is carried out and the results are compared. All the algorithms used can detect changes in data that do not have a known distribution, i.e. non-parametric distributions, which are typical for turbulent flow field signals. Measurements of the fluctuating components of the wind tunnel velocity at a specific point are considering. The signals used correspond to periodic detachments downstream of a flow control device (Gurney mini-flap) at the trailing edge of an airfoil. The results show which are the best models to use for the experimental detection of such turbulent events in the flow field.

Aspectos ecológicos parasitarios en Pleurodema nebulosum (Anura: Leptodactylidae) en la región del monte, San Juan, Argentina; Parasitic ecological aspects in Pleurodema nebulosum (Anura: Leptodactylidae) in the monte region, San Juan, Argentina Castillo, Gabriel Natalio; González Rivas, Cynthia Jesica; Acosta, Juan Carlos Pleurodema nebulosum es una especie con amplia distribución en Argentina. Se analizó el parasitismo y su relación con el sexo, perturbación humana, masa y tamaño corporal. Se identificó al nemátodo Aplectana nebulosa (Cosmocercidae) en el intestino largo. Encontramos que la abundancia media parasitaria se asoció positivamente con el tamaño (longitud hocico-cloaca), sexo macho y perturbación humana. Un mayor tamaño corporal condujo a un aumento de la abundancia media de parásitos. Los machos de ambos ambientes estaban más parasitados que hembras. La abundancia media de parásitos fue mayor en el ambiente perturbado en comparación con el control. Discutimos nuestros resultados en un contexto ecológico. Se provee información novedosa sobre el parasitismo en esta especie de anfibio en el desierto del monte de Argentina.; Pleurodema nebulosum is a species with a wide distribution in Argentina. Parasitism and its relationship with host sex, human disturbance, and body mass were assessed. The nematode Aplectana nebulosa (Cosmocercidae) was identified in the large intestine. Mean parasitic abundance was found to be positively associated with size (snout-vent length), male sex and human disturbance. Larger body size led to an increase in the mean abundance of parasites. Males in both environments were more parasitized than females. The mean abundance of parasites was higher in the disturbed environment compared to the control. We discuss our results in an ecological context. Novel information is provided on parasitism in this amphibian species in the Monte Desert, Argentina.

Novel Fluoroquinolones with Possible Antibacterial Activity in Gram-Negative Resistant Pathogens: In Silico Drug Discovery Coba Males, Manuel Alejandro; Lavecchia, Martín José; Alcívar León, Christian David; Santamaría Aguirre, Javier Antibiotic resistance is a global threat to public health, and the search for new antibacterial therapies is a current research priority. The aim of this in silico study was to test nine new fluoroquinolones previously designed with potential leishmanicidal activity against Campylobacter jejuni, Escherichia coli, Neisseria gonorrhoeae, Pseudomonas aeruginosa, and Salmonella typhi, all of which are considered by the World Health Organization to resistant pathogens of global concern, through molecular docking and molecular dynamics (MD) simulations using wild-type (WT) and mutant-type (MT) DNA gyrases as biological targets. Our results showed that compound 9FQ had the best binding energy with the active site of E. coli in both molecular docking and molecular dynamics simulations. Compound 9FQ interacted with residues of quinolone resistance-determining region (QRDR) in GyrA and GyrB chains, which are important to enzyme activity and through which it could block DNA replication. In addition to compound 9FQ, compound 1FQ also showed a good affinity for DNA gyrase. Thus, these newly designed molecules could have antibacterial activity against Gram-negative microorganisms. These findings represent a promising starting point for further investigation through in vitro assays, which can validate the hypothesis and potentially facilitate the development of novel antibiotic drugs.

Cosmogenic nuclide moraine chronologies from Patagonia: A globally synchronic response of mountain glaciers during Termination 1? Soteres, Rodrigo L.; Sagredo, Esteban; Kaplan, M.R.; Martini, Mateo Antonio; Riquelme, Fabian; Schaefer, J.M. Surface-exposure dating of moraines reveals that Patagonian glaciers fluctuated at the pulsebeat mimicked in polar ice cores from both hemispheres. These findings favor hypotheses that invoke coupled oceanic–atmospheric drivers to generate and propagate millennial-scale climate shifts during Termination 1.