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Desarrollo rural para horticultores bonaerenses. El caso del Programa Cambio Rural INTA en el partido del Pilar (provincia de Buenos Aires, Argentina)

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Desarrollo rural para horticultores bonaerenses. El caso del Programa Cambio Rural INTA en el partido del Pilar (provincia de Buenos Aires, Argentina) Feito, Maria Carolina Considerando las políticas sociales y las intervenciones de desarrollo como procesos continuos, negociados y socialmente construidos, que recogen iniciativas tanto “desde abajo” como “desde arriba”, analizamos la constitución de intervenciones en la producción hortícola bonaerense, cuyos productores demandan acciones institucionales distintas a las de otras regiones del país, por su particular historia y estrategia socioproductiva. Intentamos identificar desde qué enfoque/s se concibe el desarrollo rural en la intervención y cómo opera esto en los cambios en la producción hortícola en el programa Cambio Rural de INTA, en el partido del Pilar. Analizamos posibilidades de sustentabilidad de esta intervención, en función del modelo de gestión implementado. Utilizamos metodología cualitativa con realización de trabajo de campo etnográfico en distintos ámbitos del partido2. Considerando a los pequeños productores hortícolas como sujetos centrales de la intervención, la trayectoria y cobertura en términos de cantidad de población objetivo asistida es una fuente de legitimación de los discursos y visiones de los organismos estatales involucrados. La participación de los productores fortalece la toma de decisiones “desde abajo”, en un modelo de desarrollo focalizado en la inclusión de los productores, sumando asistencia técnica y contención social.

A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120

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A specific role of the cyanobacterial PipX factor in the heterocysts of Anabaena sp. strain PCC 7120 Valladares, Ana; Rodríguez, Virginia; Camargo, Sergio; Martínez Noël, Giselle María Astrid; Herrero, Antonia; Luque, Ignacio The PipX factor is a regulatory protein that seems to occur only in cyanobacteria. In the filamentous, heterocyst-forming Anabaena sp. strain PCC 7120, open reading frame (ORF) asr0485, identified as the pipX gene, is expressed mainly under conditions of combined-nitrogen deprivation dependent on the global N regulator NtcA and the heterocyst-specific regulator HetR. Primer extension and 5′ rapid amplification of cDNA ends (RACE) analyses detected three transcription start points corresponding to a canonical NtcA-activated promoter (to which direct binding of NtcA was observed), an NtcA- and HetR-dependent promoter, and a consensus-type promoter, the last with putative −35 and −10 determinants. Activation of pipX took place in cells differentiating into heterocysts at intermediate to late stages of the process. Accordingly, disruption of pipX led to impaired diazotrophic growth, reduced nitrogenase activity, and impaired activation of the nitrogenase structural genes. The nitrogenase activity of the mutant was low under oxic conditions, likely resulting from inefficient protection against oxygen. In line with this, the activation of the coxB2A2C2 and coxB3A3C3 operons, encoding heterocyst-specific terminal respiratory oxidases responsible for internal oxygen removal, was deficient in the pipX mutant. Therefore, the Anabaena PipX factor shows a spatiotemporal specificity contributing to normal heterocyst function, including full activation of the nitrogenase structural genes and genes of the nitrogenase-protective features of the heterocyst.

Representation theory of Yang-Mills algebras

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Representation theory of Yang-Mills algebras Herscovich Ramoneda, Estanislao Benito; Solotar, Andrea Leonor The aim of this article is to describe families of representations of the Yang-Mills algebras YM(n) (n∈N≥2) defined by A. Connes and M. Dubois-Violette. We first describe some irreducible finite dimensional representations. Next, we provide families of infinite dimensional representations of YM, big enough to separate points of the algebra. In order to prove this result, we prove and use that all Weyl algebras Ar(k) are epimorphic images of YM(n).

Improved Detection of Candida spp. fks Hot Spot Mutants by Using the Method of the CLSI M27-A3 Document with the Addition of Bovine Serum Albumin

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Improved Detection of Candida spp. fks Hot Spot Mutants by Using the Method of the CLSI M27-A3 Document with the Addition of Bovine Serum Albumin Garcia, Guillermo Manuel; Park, Steven; Perlin, David S. Echinocandins are highly bound to serum proteins, altering their antifungal properties. The addition of 50% human serum to the MIC assay improves the identification of echinocandin-resistant Candida spp. harboring fks hot spot mutations. However, this modification cannot readily be applied to the method of the CLSI M27-A3 document due to safety and standardization difficulties. The aim of this study was to evaluate commercial bovine serum albumin (BSA) as a safe and standardized alternative to human serum. A collection of 28 echinocandin-susceptible strains, 10 Candida parapsilosis sensu lato strains (with naturally reduced echinocandin susceptibility), and 40 FKS hot spot mutants was used in this work. When RPMI 1640 was used for susceptibility testing, wild-type strains and fks mutants showed MIC range overlaps ( 2, 1, and 3 2-fold-dilution steps separated these populations for anidulafungin, caspofungin, and micafungin, respectively). On the other hand, the addition of BSA to RPMI 1640 differentially increased echinocandin MIC values for these groups of strains, allowing better separation between populations, with no MIC range overlaps for any of the echinocandin drugs tested. Moreover, the use of RPMI-BSA reduced the number of fks hot spot mutant isolates for which MIC values were less than or equal to the upper limit for the wild type (very major errors) from 9, 2, and 7 with RPMI alone to 3, 0, and 3 for anidulafungin, caspofungin, and micafungin, respectively. When RPMI-BSA was used to study the susceptibility of C. parapsilosis sensu lato species to echinocandins, the strains behaved as anidulafungin- and micafungin-resistant isolates (MIC, >8 g/ml). These data support the need for a revision of the CLSI protocol for in vitro testing of echinocandin susceptibility in order to identify all or most of the fks hot spot mutants. Also, caspofungin could be used as a surrogate marker of reduced susceptibility to echinocandins.

Chemometrics-assisted fluorimetry for the rapid and selective determination of heavy polycyclic aromatic hydrocarbons in contaminated river waters and activated sludges

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Chemometrics-assisted fluorimetry for the rapid and selective determination of heavy polycyclic aromatic hydrocarbons in contaminated river waters and activated sludges Bortolato, Santiago Andres; Arancibia, Juan Alberto; Escandar, Graciela Monica The most concerned polycyclic aromatic hydrocarbons (PAHs) benzo[a]pyrene, dibenz[a,h]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene and benz[a]anthracene were simultaneously determined in the presence of other 10 interfering PAHs, applying second-order multivariate calibration to the data obtained with a flow-through optosensor interfaced to a fast-scanning spectrofluorimeter. Using a sample volume of 2.5 mL, detection limits in the range 5-115 ng L-1 were obtained in interfering samples, with a sample frequency of ca. 15 samples per hour, and with a minimum use of organic solvents, competing very favorably with chromatographic methods. The significance of this study lies in the solution of the quantitative analysis problem of six PAHs in real matrices of unknown composition. The unfolded partial least-squares/residual bilinearization (U-PLS/RBL) algorithm showed the best performance in resolving the complex studied system.

A comparative study of a novel lipophilic phthalocyanine incorporated into nanoemulsion formulations: photophysics, size, solubility and thermodynamic stability

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A comparative study of a novel lipophilic phthalocyanine incorporated into nanoemulsion formulations: photophysics, size, solubility and thermodynamic stability Garcia Vior, María Cecilia; Monteagudo, Ezequiel; Dicelio, Lelia E.; Awruch, Josefina Lipophilic tetrakis-(1-adamantylsulfanyl)phthalocyaninatozinc(II) was incorporated into two different nanoemulsion formulations with the former emulsion containing isopropyl myristate and Tween 80® and the latter glycerol monocaprylocaprate and Tween 80®. A two- to three-fold increase in water solubility was observed for the zinc phthalocyanine when incorporated into the nanoemulsions in comparison with that observed for water–DMSO (98:2). Morphological evaluation confirmed the formation of thermodynamically stable nanoemulsions in the nanosize range. The zinc phthalocyanine incorporated into the nanoemulsions was photo-stable over the irradiation times studied and both formulations displayed excellent singlet oxygen generation ability with high values of quantum yield of singlet oxygen production of up to 0.58. In addition, the viscosity of the nanoemulsions ranged from 30.2 to 323.9 cP. The foregoing formulations are considered to be strong candidates for parenteral administration of the zinc phthalocyanine in photodynamic therapy clinical trials.

Contra deum Deus ipse: o núcleo êxtimo da teologia política de Slavoj Žižek

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Contra deum Deus ipse: o núcleo êxtimo da teologia política de Slavoj Žižek Senci, Carlos Maximiliano O presente trabalho começa com uma breve síntese da s origens inteletuais da teologia política de Slavoj Žižek. A seguir, o texto se divide em dua s seções. Na primeira seção, explora aquilo que Žižek entende sob o título de “materialismo”. C om efeito, o texto oferece uma definição identificando o Materialismo zizekiano com o Ato ra dical de fidelidade ao Vazio. Na segunda seção, descreve-se a qualificação própria de “paulinismo”. Para isso enfatizamos a discrepância com a aproximação do paulinismo que oferece Alain Badiou no seu livro Saint Paul: La fondation de l’universalisme. O artigo conclui comparando e contrastando as tentativas de Žižek e Schmitt para reconstruir uma teologia política.; We will begin with a brief synthesis of the intelle ctual origins of Slavoj Žižek 's political theology. Therafter, the text consists of two main sections. In the first section, we will explore what Žižek understands under the label ‘materialism’. Indeed, a definition is provided identifying Zizekian Materialism with the radical Act of fidelity to the Void. In the second section, the qualification of ‘paulinism’ is descri bed. It is essential for our purposes on this point to stress the disagreement between Žižek’s and the approach to the same problem in Alain Badiou’s Saint Paul. La fondation de l’universalisme. This essay will then conclude by comparing and contrasting Žižek and Schmitt's attempts to rebuild a political theo logy.

American bullfrog invasion in Argentina: Where should we take urgent measures?

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American bullfrog invasion in Argentina: Where should we take urgent measures? Nori, Javier; Akmentins, Mauricio Sebastián; Ghirardi, Romina; Frutos, Nicolás; Leynaud, Gerardo Cristhian Argentina is the country with the most geographically extended biologicalinvasion of the American bullfrog (Lithobates catesbeianus) in South America after Brazil.Here, we used a maximum entropy ecological niche modeling algorithm (using records ofthe native range of American bullfrog) to project the model onto the whole of Argentina.We determined the most suitable habitats for this invasive alien species and where weconsider urgent measures should be taken. Our projections showed good agreement withknown feral populations of American bullfrog in Argentina. By implementing the ??Multivariate Environmental Similarity Surface?? analysis, we be able to determine thatfactors such as low precipitations or highest altitudes could be limiting the species? abilityto invade the west and south of the country. We suggest that strategies should focus ondetecting established feral populations of the American bullfrog and preventing furtherintroductions or range expansion of feral populations in the northeast portion of thecountry. Lastly, we report a new feral population of bullfrogs in Argentina.

Anti-inflammatory effect of Lithrea molleoides extracts and isolated active compounds

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Anti-inflammatory effect of Lithrea molleoides extracts and isolated active compounds Gorzalczany, Susana Beatriz; López, Paula Gladys; Acevedo, C.; Ferraro, Graciela Ester Aim of the study: In this study the anti-inflammatory activity of aqueous, dichloromethane (CH2Cl2) and methanolic (MeOH) extracts and two major compounds isolated from Lithrea molleoides (Vell.) Engl. (Anacardiaceae) were evaluated. Materials and methods: Two classical experimental models were used, carrageenan-induced rat paw edema and 12-O-tetradecanoylphorbol-13 acetate (TPA) induced mouse ear edema. Results: MeOH extracts exhibited a significant systemical anti-inflammatory effect in the carrageenan (inhibition of 46% at 3 h) and in the TPA-ear edema test (inhibition of 21%). The presence of methyl gallate (inhibition of 63% in TPA ear edema), as one of the main compounds in the active fraction from MeOH extract may be explained the effect observed. Also, 1,3-dihydroxy-(Z,Z)-5-(tridec-4,7ˇıdienyl) benzene obtained from CH2Cl2 extract showed a significant topical anti-inflammatory activity (inhibition of 68%). Furthermore, no signs of toxicity were observed with doses up to 3 g/kg in an acute toxicity assay. Conclusions: The results of this study present evidence that Lithrea molleoides given either systemically or topically has anti-inflammatory properties.

De la revisión del artista del pueblo al cuestionamiento institucional: Lecturas sobre Guillermo Facio Hebequer

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De la revisión del artista del pueblo al cuestionamiento institucional: Lecturas sobre Guillermo Facio Hebequer Dolinko, Silvia Esther El enfoque del grabado en su estrecha vinculación con la difusión de contenidos sociales y políticos es posiblemente uno de los aspectos más reconocidos de su propia tradición. A partir del efecto movilizador que se puede desprender de la circulación seriada o plural de discursos artísticos de cuestionamiento y oposición, la idea del grabado como arte comprometido o de denuncia social constituye un topos a partir del cual se ha entendido el lugar o la función de esta producción. Las imágenes de algunos gráficos alemanes como Käthe Kollwitz, Max Beckmann, Otto Dix o George Grosz, del belga Frans Masereel, o de los mexicanos José Guadalupe Posada o los miembros del Taller de Gráfica Popular forman parte de la idea del grabado como vehículo para la difusión del discurso social o comprometido en distintos momentos del siglo XX.

Feeding Patterns of the Aquatic Grasshopper Cornops aquaticum (Bruner)

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Feeding Patterns of the Aquatic Grasshopper Cornops aquaticum (Bruner) Capello, Soledad; de Wysiecki, Maria Laura; Marchese Garello, Mercedes Rosa The aquatic grasshopper Cornops aquaticum (Bruner) is native to South America and inhabits lowlands from southern Mexico to Central Argentina and Uruguay. This grasshopper is host-specific to aquatic plants of the genera Eichhornia and Pontederia. The objectives of this study were to analyze the feeding patterns of the aquatic grasshopper C. aquaticum in relationship to development stages and sex and to determine the food consumption rate in their host plant, E. crassipes. Samples were collected from April 2006 to May 2007 in different floodplain lakes of the Middle Parana River. The average consumption was greater in the females (0.127 g food/day ± 0.051) than in the males (0.060 g food/day ± 0.025). The feces of 361 nymphs and adults of this locust were examined and the most common tissue fragments found were of the water hyacinth (Eichhornia crassipes). In the initial nymphal stages (I, II and III), an exclusive consumption of E. crassipes was registered, while in the IV and V stages the choice included also other macrophytes. In summary, C. aquaticum presents polyphagy in the field, feeding on six macrophytes of different classes and families.

Developmental plasticity in covariance structure of the skull: Effects of prenatal stress

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Developmental plasticity in covariance structure of the skull: Effects of prenatal stress Gonzalez, Paula Natalia; Hallgrimsson, Benedikt; Oyhenart, Evelia Edith Environmental perturbations of many kinds influence growth and development. Little is known, however, about the influence of environmental factors on the patterns of phenotypic integration observed in complex morphological traits. We analyze the changes in phenotypic variance-covariance structure of the rat skull throughout the early postnatal ontogeny (from birth to weaning) and evaluate the effect of intrauterine growth retardation (IUGR) on this structure. Using 2D coordinates taken from lateral radiographs obtained every 4 days, from birth to 21 days old, we show that the pattern of covariance is temporally dynamic from birth to 21 days. The environmental perturbation provoked during pregnancy altered the skull growth, and reduced the mean size of the IUGR group. These environmental effects persisted throughout lactancy, when the mothers of both groups received a standard diet. More strikingly, the effect grew larger beyond this point. Altering environmental conditions did not affect all traits equally, as revealed by the low correlations between covariance matrices of treatments at the same age. Finally, we found that the IUGR treatment increased morphological integration as measured by the scaled variance of eigenvalues. This increase coincided and is likely related to an increase in morphological variance in this group. This result is expected if somatic growth is a major determinant of covariance structure of the skull. In summary, our findings suggest that environmental perturbations experienced in early ontogeny alter fundamental developmental processes and are an important factor in shaping the variance-covariance structure of complex phenotypic traits.

Association of bovine chromosome 5 markers with birth and weaning weight in Hereford cattle raised under extensive conditions

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Association of bovine chromosome 5 markers with birth and weaning weight in Hereford cattle raised under extensive conditions Rogberg Muñoz, Andres; Melucci, Lilia Magdalena; Prando, Alberto José; Villegas Castagnasso, Egle Etel; Ripoli, María Verónica; Peral Garcia, Pilar; Baldo, Andres; Añon, Maria Cristina; Giovambattista, Guillermo Genetic markers have been used to assess the association of economically important traits with cattle under intensive feeding conditions; however, there is still the need to ascertain the usefulness of these markers under extensive production systems. Bovine chromosome 5 has been widely studied because several QTLs have been detected. Microsatellite BP1 neighboring the Myogenic factor 5 gene (Myf5), and microsatellites ETH10, IGF1 and RM029 near Insulin-like Growth Factor 1 gene (IGF1), were selected to establish their association with BLUPs (Best Linear Unbiased Predictor) for direct Birth Weight (dBW), direct Weaning Weight (dWW) and maternal Weaning Weight (mWW). Two herds were used for this objective, one commercial and the other experimental. Associations (P ≤ 0.05) between dWW and all BTA5 loci (BP1, ETH10, IGF1, and RM029) were detected. Additional associations were observed between mWW and BP1. dBW was significantly associated (P ≤ 0.05) with ETH10 genotypes and with the interaction IGF1*Herd. In particular region near BP1 could be contributing to the rare positive correlation between dWW and mWW previously found in the INTA Balcarce Station experimental herd. We confirmed marker associations with growth traits in two BTA5 regions close to previously reported QTL obtained in intensive feeding conditions; these regions affect dBW, dWW and mWW in a pasture-based system.

Improvements on a laser scattering technique for droplet size measurements applied to a gas-liquid separation equipment

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Improvements on a laser scattering technique for droplet size measurements applied to a gas-liquid separation equipment Marchetti, Jorge Mario; Svendsen, Hallvard Fjøsne The knowledge of droplet size distributions in a gas-liquid separation equipment is of high relevance due to the importance of removal efficiency in these systems. Different techniques could be used to measure droplet size, being one of them the diffraction of a laser beam. The laser is located behind glasses, being the formation of droplets on the glasses one of the main problems encountered when using this technique. Due to this major problem, different innovative solutions have been proposed and implemented to the gas-liquid separation column in order to obtain satisfactory results. A shutter mechanism, a purge gas and combination of these two solutions were tested. It was shown that the modified technique is suitable for liquid droplet measurements under ambient conditions. It has been also shown that the combination of these two solutions reduced considerably the amount of droplets that interacts with the glasses, allowing getting better data.

Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia

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Hypothermia Prevents Nitric Oxide System Changes in Retina Induced by Severe Perinatal Asphyxia Rey Funes, Manuel; Ibarra, Mariano Esteban; Dorfman, Verónica Berta; Serrano, Julia; Fernández, Ana Patricia; Martínez Murillo, Ricardo; Martínez, Alfredo; Coirini, Hector; Rodrigo, José; Loidl, Cesar Fabian One-third of asphyctic neonates develop long-term neurological injuries, including several degrees of ischemic proliferative retinopathy (IPR) such as retinopathy of prematurity (ROP). Given that the retina is altered by perinatal asphyxia, our aim was to study the effects of nitric oxide (NO) in the retina in order to analyze its impact on the retinal injury. Application of hypothermia was evaluated as preventive treatment. Sprague-Dawley rats were subjected to perinatal asphyxia [either at 37°C (PA group) or at 15°C (HYP group)]. Full-term rats were used as controls (CTL). A significantly increased activity of both constitutive NO synthase (nNOS, Ca2+-dependent) and inducible NO synthase (iNOS, Ca2+-independent) was observed in PA retinas from 21 days old up to 60 days old with respect to age-matched CTL, with a significant increase along the time course in the PA. nNOS was immunolocalized at amacrine, horizontal, and ganglion cells of the PA group, with a significant increase in relative optical density (R.O.D.), cellular area, and number of cells. iNOS immunoreactivity was observed in the inner nuclear layer and in the internal Müller cell processes of PA, with a significant increase in R.O.D. and colocalizing with GFAP in the 60-day-old PA group. Six nitrated protein species were increased in retinas from PA rats. Nitrotyrosine immunoreactivity showed a localization similar to that of iNOS, with increased R.O.D. in the PA group and colocalization with GFAP in 60-day-old animals. HYP prevented all the changes observed in PA rats. Although the NO system displays changes induced by hypoxia-ischemia, hypothermia application shows a strong protective effect.

Fluoride removal from water by chitosan derivatives and composites: a review

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Fluoride removal from water by chitosan derivatives and composites: a review Miretzky, Patricia; Fernandez Cirelli, Alicia Fluoride is an essential element, indispensable for maintenance of dental health. Nevertheless, fluoride concentrations in drinking water above 1.5 mg L−1 may be detrimental to human health. Many methods have been developed for removing excessive fluoride from drinking water, adsorption seems to be an effective, environmentally friendly and economical one. Since the sorption capacity of fluoride below 2 mg L−1 on most conventional adsorbents is not satisfactory, much effort has been devoted to develop new and cost-effective fluoride adsorbents. This review reports the recent developments in the F− removal in water treatment, using chitosan derivatives and composites in order to provide useful information about the different technologies. When possibly the adsorption capacity of chitosan derivatives and composites under different experimental conditions is reported to help to compare the efficacy of the fluoride removal process. A comparison with the adsorption capacity of other low cost adsorbents is also tabled.

Puertos sin barcos. El espacio urbano en el proceso de reconversión de usos del área portuaria de Santa Fe

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Puertos sin barcos. El espacio urbano en el proceso de reconversión de usos del área portuaria de Santa Fe; Ports without ships. The urban space in the process of restructuring the uses of the port area in Santa Fe Fedele, Javier Los puertos fuera de uso son una de las áreas urbanas donde cabalmente pueden ser interpretados los cambios en las ciudades actuales. Estos son producto de mecanismos de planificación urbana como de recursos arquitectónicos empleados, que intervienen confluyentemente en el proceso de definición de estas áreas y producen resultados espaciales que caracterizan los procesos urbanos actuales. En el marco de esta problemática, el artículo aborda las estrategias de organización de las nuevas actividades urbanas en el Puerto de Santa Fe, como sus modos de gestión urbana y sus recursos arquitectónicos determinan la fisonomía, uso e imagen del espacio urbano.; Disused ports could be considered the urban areas where the changes in modern cities could be best fully interpreted. These are the result of urban planning mechanisms as well as of the use of architectural resources, both taking part in a confluent way in the process of defining these areas, and resulting in typically characteristic spaces of the current urban processes. Within the framework of this situation, the article addresses the strategies used to organize the new urban activities in the port of Santa Fe, and how their urban management models and their architectural resources determine shape, use and image of urban space.

Recycling or organic waste generated in gastronomic activity

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Recycling or organic waste generated in gastronomic activity Chalón, Myriam; Terán, Victoria; Arena, Mario Eduardo; Oliszewky, Ruben; Gonzalez, Silvia Nelina The solid waste generated in gastronomic activity, in our country, represents, a health problem. The solution could begin with a differential collection of these wastes and their recycling for specific purposes. The aim of this study was to design a culture medium for bacteria and fungi, whose nutrients came from organic waste generated in restaurants. The average percentage composition was: Crude protein 0.77 ± 0.18, dry matter: 1.53 ± 0.05, carbohydrates: 1.00 ± 0.03 and ash: 0.95 ± 0.14. This medium was used for biomass (bacteria and yeasts) and no significant differences (p > 0.05) were found in relation to commercial culture media, when were found in relation to commercial culture media, when Lactobacillus plantarum ATCC7469, Lactobacillus rhamnosus O236, Lactobacillus sp L46, Enterococcus sp C999, Staphylococcus aureus E2, Shigella sonnei S3, Salmonella enteritidis S4, Saccharomyces cerevisiae L1 and Schizosaccharomyces sp L2 were cultivated. The only organism included in this study, unable to develop in the culture media designed was Streptococcus Pa E1. The Streptococcus genus requires, for an optimal performance, complex media enriched with blood. Since the purpose of our study was to consider a minimal medium, blood addition was not considered, even knowing the nutritional requirements of this genus of bacteria. Our results allowed infer the conversion of organic waste in an alternative culture medium with a good yield in biomass. In addition, this medium showed some characteristics as accessibility (in any region of the country can be elaborated), low cost because, for processing, wastes generated in quantities and often highly were required; versatility (allows the growth of Gram-negative and Gram positive bacteria and also yeast). The single cell proteins have an important nutritional value due to high content of vitamins and lipids. Moreover these proteins present, in general, a whole set of essential amino acids. For this reason, yeast cells are usually used in animal feeding. The studies about technological process for elaborating the designed medium are in progress.

Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna

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Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna Dib, Julian Rafael; Maldonado, Marcos Javier; Thürmer, Andrea; Schuldes, J.; Rolf, Daniel; Farias, Maria Eugenia Living or ‘modern’ stromatolites are mostly found in hypersaline lakes and marine lagoons with extreme conditions. The most recently found and as yet oldest living stromatolites were discovered by our lab in February 2009, in Laguna Socompa and Tolar Grande, in Salta province, Argentina; at about 4,000 m. A collection of Archaea isolated from these stromatolites has been established. Among the domain of the Archaea, haloarchaea are considered suitable for the study of archaeal genetics and a rich source of plasmids as well. The main of this work was the isolation, sequencing and bioinformatic analysis of plasmid pAT23 from Halopeiger sp. strain AT23, a haloarchaea isolated from living stromatolites. Plasmid DNA was isolated by QIAGEN Plasmid Mini Kit and sequenced by Sanger technology followed by standard bioinformatic analyses. DNA sequence of a novel plasmid pAT23 (7,843 bp) from haloarchaeal Halopeiger sp. AT23 was determined. A total of 15 open reading frames (ORFs) were identified; putative functions were assigned to 4 of them among others with no annotatable function. Regarding the replication region, one ORF was found with significant homology to the RepA of Halorubrum marismortui. Analysis of the nucleotide sequence also revealed the presence of type 1 fimbriae regulatory protein FimB as well as a protein PemK, responsible for mediating cell death through inhibiting protein synthesis through the cleavage of single-stranded RNA. The largest ORF encodes a putative RloA protein which shares homology with Lutella nitroferrum. pAT23 is a novel plasmid isolated from the halophilic archaeon Halopeiger sp. strain AT23, which has little homology with those haloarchaeal plasmids reported so far. To our knowledge this is the first report of the sequencing of a plasmid for the genus Halopeiger.

Interacción de fosfolípidos con anillos mono- THF por FTIR

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Interacción de fosfolípidos con anillos mono- THF por FTIR Di Toto Blessing, Lilian Edith; Ramos, Juan; Díaz, Sonia Beatriz; Ben Altabef, Aida; Bardon, Alicia del Valle; Brovetto, Margarita; Seoane, Gustavo; Neske, Adriana Estudios previos realizados en nuestro laboratorio por FTIR, mostraron que las acetogeninas annonáceas (ACG) mono-THF, producen deshidratación del entorno del grupo carbonilo y deshidratación con posterior formación de uniones de H en el entorno del grupo fosfato. Resultados de las simulaciones de DM corroboraron los datos experimentales. Se observó que la ACG mono-THF interactúa tanto con el grupo fosfato como con los grupos carbonilo del fosfolípido, desalojando moléculas de agua del entorno y formando fuertes uniones de hidrógeno con el grupo fosfato.

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