Association of an IRF3 putative functional uORF variant with resistance to Brucella infection: A candidate gene based analysis of InDel polymorphisms in goats Rossi, Ursula Amaranta; Hasenauer, Flavia Carolina; Caffaro, Maria Eugenia; Raschia, Maria Agustina; Maurizio, Estefanía; Cortez, Héctor Sergio; Neumann, Roberto; Poli, Mario Andres; Rossetti, Carlos Alberto Brucellosis is an important zoonotic disease caused by infection with Brucella spp. It generates major economic losses in livestock production worldwide. Goats are the principal hosts of B. melitensis, the main infection agent of caprine and human brucellosis. The selection of resistance-related genes is considered one of the best longterm means to improve control to bacterial infection in domestic ruminants. We performed a candidate gene association study to test if six short insertion/deletion polymorphisms (InDels) at bacterial-infection related genes influence the resistance to Brucella infection in female creole goats. InDels (IRF3-540: rs660531540, FKBP5-294: rs448529294, TIRAP-561: rs657494561, PTPRT-588: rs667380588, KALRN-989: rs667660989 and RAB5a-016: rs661537016) were resolved by PCR-capillary electrophoresis in samples from 64 cases and 64 controls for brucellosis. Allelic frequencies were significantly different between cases and controls at IRF3-540 and KALRN-989 (p = 0.001 and 0.005). Indeed, the minor alleles (a and k) at InDels IRF3-540 and KALRN-989 were more frequent among controls than cases, providing evidence that these alleles confer protection against Brucella infection. Moreover, IRF3-540 a-containing genotypes (Aa and aa) were associated with absence of Brucella-specific antibodies in goats (p = 0.003; OR = 3.52; 95% CI = 1.55–7.96), and more specifically, a-allele was associated with resistance to Brucella infection in a dose-dependent manner. Also, we observed that the IRF3-540 deletion (a-allele) extends a conserved upstream ORF by 75 nucleotides to the main ORF, and thus it may decrease gene expression by reducing translation efficiency from the main ORF. These results suggest a potential functional role of IRF3-540 deletion in genetic resistance to Brucella infection in goats.

Multifunktionale Aluminiumoberflächen durch laserinduzierte Verfahren Milles, Stephan; Soldera, Marcos Maximiliano; Voisiat, Bogdan; Nitschke, M.; Baumann, Robert; Lasagni, Andrés Fabián Laserlicht bietet, durch seine hohe gebündelte, Energie eine Vielzahl von Einsatzmöglichkeiten. Durch verschiedenste Laserverfahren können filigrane Mikrostrukturen hergestellt, mit denen sich die Benetzung und Vereisung von Aluminiumoberflächen beeinflussen lässt.

Los derechos de la niñez migrante en América Latina y el Caribe: Entre la vulneración y la falta de garantías Parodi, Lucía Hipatía La inestabilidad institucional, política, social y económica en ciertos países de la región latinoamericana ha producido una diversificación de flujos migratorios con una creciente movilidad intrarregional. De esta enorme población que recorre los rincones de nuestra región, un colectivo especialmente vulnerable despierta nuestra atención: la infancia migrante. Este trabajo se propone analizar la particular situación en la que se encuentran los niños migrantes en América Latina y Caribe, los derechos que a éstos les asisten y la función primordial del Estado como garante de la protección integral de la infancia.

Rational design of polymer-lipid nanoparticles for docetaxel delivery Albano, Juan Manuel Ricardo; Ribeiro, Lígia Nunes de Morais; Couto, Verônica Muniz; Barbosa Messias, Mariana; Rodrigues da Silva, Gustavo Henrique; Breitkreitz, Márcia Cristina; de Paula, Eneida; Pickholz, Mónica Andrea In this work, a stable nanocarrier for the anti-cancer drug docetaxel was rational designed. The nanocarrier was developed based on the solid lipid nanoparticle preparation process aiming to minimize the total amount of excipients used in the final formulations. A particular interest was put on the effects of the polymers in the final composition. In this direction, two poloxoamers -Pluronic F127 and F68- were selected. Some poloxamers are well known to be inhibitors of the P-glycoprotein efflux pump. Additionally, their poly-ethylene-oxide blocks can help them to escape the immune system, making the poloxamers appealing to be present in a nanoparticle designed for the treatment of cancer. Within this context, a factorial experiment design was used to achieve the most suitable formulations, and also to identify the effects of each component on the final (optimized) systems. Two final formulations were chosen with sizes < 250 nm and PDI < 0.2. Then, using dynamic light scattering and nanotracking techniques, the stability of the formulations was assessed during six months. Structural studies were carried on trough different techniques: DSC, x-ray diffraction, FTIR-AR and Molecular Dynamics. The encapsulation efficiency of the anticancer drug docetaxel (> 90%) and its release dynamics from formulations were measured, showing that the polymer-lipid nanoparticle is suitable as a drug delivery system for the treatment of cancer.

Development of a third-generation biosensor to determine sterigmatocystin mycotoxin: An early warning system to detect aflatoxin B1 Díaz Nieto, César Horacio; Granero, Adrian Marcelo; Garcia, D.; Nesci, Andrea Verónica; Barros, Germán Gustavo; Zon, María Alicia; Fernández, H. A third-generation enzymatic biosensor was developed to quantify sterigmatocystin (STEH). It was based on a glassy carbon electrode modified with a composite of the soybean peroxidase enzyme (SPE) and chemically reduced graphene oxide. The optimal conditions to construct the biosensor were obtained through an experimental design based on the response surfaces methodology. The experiments were performed in 0.1 mol L−1 phosphate buffer solution, pH 5. Amperometric measurements were carried out at − 0.09 V vs Ag/AgCl (3 mol L−1 NaCl). The biosensor showed a lineal response in the concentration range from 6.9 × 10−9 to 5.0 × 10−7 mol L−1. The limit of detection was 2.3 × 10−9 mol L−1 for a signal: noise ratio of 3: 1. Values of the apparent Michaellis-Menten constant, KM app, obtained by using both Lineweaver-Burk and Eadi-Hofstee methods were (1.5 ± 0.2) × 10−6 and (1.2 ± 0.2) × 10−6 mol L−1, respectively. STEH was analyzed in corn samples spiked with STEH, with an average recovery of 96.5%. The biosensor was also used to determine STEH in corn samples inoculated with the Aspergillus flavus fungus, which is an aflatoxins producer. Considering that STEH is a precursor of aflatoxin B1 (AFB1) in its biological transformation, its decrease over time was related to the production of AFB1. The STEH concentration determined using the biosensor was in very good agreement with that determined by HPLC.

Eigenvalue bounds and spectral asymptotics for fractal Laplacians Pinasco, Juan Pablo; Scarola, Cristian In this work we present Lyapunov type inequalities for generalized one dimensional Laplacian operators defined by positive atomless Borel measures. As applications, we present lower bounds for the first eigenvalue when the measure is a Bernoulli convolution, with or without overlaps. Also, for symmetric Bernoulli convolutions we obtain two sided bounds for higher eigenvalues, and we recover the asymptotic growth of the spectral counting function by elementary means without using the Renewal Theorem. We also consider the Laplacian on the Sierpinsky gasket and other similar fractals, and we deduce a lower bound of their eigenvalues from a Lyapunov type inequality.

Molecular-level Understanding of the Rate-determining Step in Esterification Reactions Catalyzed by H-ZSM-5 Zeolite. An Experimental and Theoretical Study Gomes, Glaucio José; Zalazar, Maria Fernanda; Arroyo, Pedro Augusto; Scremin, Fernando R.; Costa, Michelle Budke; Bittencourt, Paulo R. S.; Lindino, Cleber A.; Peruchena, Nelida Maria Transformation of biomass into renewable energy products is currently one of the most promising technologies for dropping dependence on fossil fuels. Biodiesel production may improve with the use of heterogeneous catalysts, such as zeolites. In this work, computational calculations in conjunction with thermal analysis (TGA), evolved gases analysis (EGA) and IR spectroscopic studies were used in order to obtain a more detailed information on the adsorption mechanism involved in the rate-determining step of esterification reactions inside the H-ZSM-5 zeolite. ATR-FTIR spectra showed a molecular adsorption of MeOH and acetic acid (AcOH) on the H-ZSM-5 surface, IR spectrum of AcOH showed perturbations in the region of the bands relative to the carbonyl group C=O, indicating molecular interactions by the adsorption processes, as predicted by theoretical calculations. Adsorption of a single molecule of AcOH by C=O in a single adsorption step followed by coadsorption of MeOH is more stable than adsorption involving the zeolite proton sharing, however energies values suggested competitiveness between both mechanisms. Interactions between the adsorbed molecules and the lattice structure are crucial in controlling the adsorption observed experimentally.

Polo-like kinase 1 inhibition as a therapeutic approach to selectively target BRCA1-deficient cancer cells by synthetic lethality induction Carbajosa González, Sofía; Pansa, Maria Florencia; Paviolo, Natalia Soledad; Castellaro, Andrés Marcos; Andino, Diego Leonardo; Nigra, Ayelén Denise; García, Iris Alejandra; Racca, Ana Cristina; Rodriguez, María Celeste; Angiolini, Virginia Andrea; Guantay, Maria Laura; Villafañez, Florencia; Federico, Maria Belén; Rodríguez, Lucía; Caputto, Beatriz Leonor; Drewes, Gerard; Madauss, Kevin P.; Gloger, Israel; Fernandez, Elmer Andres; Gil, German Alejandro; Bocco, Jose Luis; Gottifredi, Vanesa; Soria, Ramiro Gaston Purpose: BRCA1 and BRCA2 deficiencies are widespread drivers of human cancers that await the development of targeted therapies. We aimed to identify novel synthetic lethal relationships with therapeutic potential using BRCA-deficient isogenic backgrounds. Experimental Design: We developed a phenotypic screening technology to simultaneously search for synthetic lethal (SL) interactions in BRCA1- and BRCA2-deficient contexts. For validation, we developed chimeric spheroids and a dualtumor xenograft model that allowed the confirmation of SL induction with the concomitant evaluation of undesired cytotoxicity on BRCA-proficient cells. To extend our results using clinical data, we performed retrospective analysis on The Cancer Genome Atlas (TCGA) breast cancer database. Results: The screening of a kinase inhibitors library revealed that Polo-like kinase 1 (PLK1) inhibition triggers strong SL induction in BRCA1-deficient cells. Mechanistically, we found no connection between the SL induced by PLK1 inhibition and PARP inhibitors. Instead, we uncovered that BRCA1 downregulation and PLK1 inhibition lead to aberrant mitotic phenotypes with altered centrosomal duplication and cytokinesis, which severely reduced the clonogenic potential of these cells. The penetrance of PLK1/BRCA1 SL interaction was validated using several isogenic and nonisogenic cellular models, chimeric spheroids, and mice xenografts. Moreover, bioinformatic analysis revealed high-PLK1 expression in BRCA1-deficient tumors, a phenotype that was consistently recapitulated by inducing BRCA1 deficiency in multiple cell lines as well as in BRCA1-mutant cells. Conclusions: We uncovered an unforeseen addiction of BRCA1-deficient cancer cells to PLK1 expression, which provides a new means to exploit the therapeutic potential of PLK1 inhibitors in clinical trials, by generating stratification schemes that consider this molecular trait in patient cohorts.

The contribution of the pairing field to the transfer of two nucleons Bes, Daniel Raul; Civitarese, Enrique Osvaldo The treatment of pairing interactions in systems with a finite number of particles, like the atomic nucleus, was originally performed by applying the BCS transformations in the energy representation. An alternative method, formulated by de Gennes, introduces explicitly the radial dependence of the BCS-type solutions of the pairing force problem for superconductors. In this paper we explore the consequences of the use of de Gennes's formalism to calculate the pairing contribution to two-nucleon transfer amplitudes.

Effect of PAA-coated magnetic nanoparticles on the performance of PVA-based hydrogels developed to be used as environmental remediation devices Sanchez, Laura M.; Actis, Daniel Guillermo; Gonzalez, Jimena Soledad; Mendoza Zélis, Pedro; Alvarez, Vera Alejandra In the present work, the effect of the incorporation of polyacrylic acid (PAA)–coated magnetic nanoparticles (MNPs) on the performance of polyvinyl alcohol (PVA)–based hydrogels for water remediation was studied. Ferrogels from PVA and PAA-coated MNPs were prepared through the ecocompatible freezing–thawing physical cross-linking method, and then they were completely characterized. Two different lab-made PAA-coated iron oxide MNPs, characterized in a previous work, were prepared by coprecipitation method from two different low PAA molecular weights, Mw 1800 g/mol and 5000 g/mol. The effect of MNP content and the PAA Mw on ferrogel final properties was determined. In addition, adsorption of methylene blue (MB) and cadmium (Cd+2) was carried out to analyze the possible application of the developed materials as environmental remediation devices. The capture of a ferrogel by an external field occurs due to the force that the field gradient exerts on single magnetic particles, which is then transferred onto the polymer matrix. This force was measured as a function of the distance to a permanent magnet, and the condition to magnetically recover the sample was established. The results obtained demonstrated that the ferrogels presented in this work are able to adsorb heavy metals and then be magnetically separated.

Nuevo banco de ensayo de pérdida de carga en componentes hidráulicos Reyna, Estela Eugenia; Bracco, Mauricio; Gañan, Nicolas Alberto; Alasino, Noelia Pia Ximena Diseño y Construcción de un nuevo Banco de pruebas para pérdidas de carga en componentes hidráulicos para reemplazar el anterior que estaba obsoleto. Su diseño fue estudiado para que sea versátil, robusto y didáctico. Fue construido para ser utilizado para trabajos prácticos y de investigación por 9 cátedras de la Facultad de Ciencias Exactas Físicas y Naturales. Para construirlo, se utilizaron materiales actuales en el mercado. Gran cantidad de puntos de toma de presión. Diferentes variantes para mediciones de caudal. Variación de caudales por medio de variador electrónico de frecuencia de la bomba. Válvulas de acople rápido con medias uniones dobles a los fines de poder intercambiar tramos a estudiar. Toma de presiones mediante sensores electrónicos y recolección de datos por medio de software provisto por el fabricante para visualizar e interpretar información analítica y gráficamente con gran simplicidad.

Naturalismo e identidad personal: la propuesta del “yo” narrativo de Dennett León, Malena En la teoría narrativista de Daniel Dennett, el “yo” es entendido como una abstracción definida por un conjunto de atribuciones e interpretaciones, propias y ajenas, que conforman la biografía del cuerpo viviente del que es el "centro de gravedad narrativa" (Dennett, 1988, 1991, 1992). Dicha teoría narrativa del “yo” constituye una de las muchas instancias en las que este filósofo aborda el proyecto de explicar la mente y dar cuenta de la singularidad humana desde una perspectiva naturalista. Un rasgo distintivo su filosofía es el lugar que en ella ocupa la teoría de la evolución de las especies.En este trabajo pretendo evaluar la potencia de la concepción narrativista del “yo” de Dennett a la hora de cumplir con lo que voy a considerar uno de los desafíos principales para perspectivas naturalistas como las de este tipo; a saber: construir explicaciones que eviten brindar una imagen excesivamente deflacionada de las características y capacidades de la mente humana, y que, por otra parte, no incurran en el error de presentar a las mismas como originándose a partir de una suerte de salto milagroso que proponga una excepción al principio darwiniano según el cual los productos del mundo natural son, en último término, el resultado de una actividad mecánica no inteligente y sin motivo, y no de una mente o principio superior. Con ese objetivo, en una primera instancia, voy a realizar una breve reconstrucción de la teoría dennettiana del “yo” y defender que la versión narrativista de Dennett se caracteriza por proponer una explicación de la elaboración de las narraciones que nos constituyen como “yoes” que incluye componentes de carácter biológico. La misma nos devuelve una imagen en la que no aparecemos como creadores conscientes, antes bien, somos, según la sugerencia de Dennett, como arañas exudando una tela de forma relativamente instintiva. Estas parecen ser el tipo de implicancias que irían en contra de nuestras visiones folk acerca de nosotros mismos. Con el objeto de negar esta presunta implicancia, en el segundo apartado voy a intentar poner en relación esta teoría con lo que Dennett dice sobre el rol del lenguaje en la inteligencia. Por último, voy a intentar justificar por qué este enfoque permite explicar la posibilidad de intervenciones deliberadas en la constitución de nuestros “yoes” sin renunciar a su naturalismo.

Une bureaucratie para-étatique mouvante: La production locale du Welfare des précaires en Argentine à l’ère du capitalisme postindustriel; A shifting para-state bureaucracy: Local production of precarious welfare in argentina in the post-industrial capitalist era Vommaro, Gabriel Alejandro Depuis les années 2000, des politiques d’assistance aux catégories les plus instables des classes populaires se consolident en Argentine comme dans la plupart des pays de l’Amérique latine. Une partie de ces politiques est gérée de manière conjointe par des dirigeants et des activistes locaux de partis et de mouvements sociaux. Il s’agit d’une véritable coproduction de l’intervention sociale de l’État dans les quartiers populaires. Basé sur un travail ethnographique dans trois quartiers populaires argentins, cet article analyse la production quotidienne des politiques sociales et notamment les activités de ses médiateurs qui jouent le rôle de bureaucrates para- étatiques. Issus eux-mêmes des classes populaires, ils sont à la fois des relais – pas toujours institutionnalisés – de l’État dans les quartiers populaires et des courroies de transmission des demandes des habitants de ces milieux face à l’État. L’analyse montre que le statut instable et les conditions de travail de ces bureaucrates, ainsi que leurs relations de dépendance avec les fonctionnaires étatiques les habilitant à distribuer des biens publics sont centraux pour comprendre les traits fondamentaux du rapport à l’État des couches les plus instables des classes populaires comme des logiques de fonctionnement du Welfare dans ces quartiers.; Since the 2000s, policies of assistance to unstable sectors of the popular classes are consolidating in Argentina as in most Latin American countries. Local leaders and political party and social activists have participated in the administration of some parts of these policies. Accordingly, genuine coproduction of the social intervention of the State in popular districts has taken place. Based on ethnographic work in three Argentine neighborhoods, this article analyzes the everyday production of social policies on the ground and in particular the role of its mediators: para-state bureaucrats. These actors also come from the popular classes, and they are both relays – that are not always institutionalized – of the State in the popular districts on the one hand, and belts of transmission of the demands of the inhabitants of these districts back to the State on the other. We argue that the status and working conditions of these bureaucrats, as well as their reliance on unstable political relations with state officials to ensure the continuity of access to goods to be distributed at the local level, make it possible to grasp fundamental features of the relationship between the state and popular classes, as well as of the forms of welfare provided by the former.

Reply: Biogenic structures of unioniform bivalves in wet-interdune deposits (Late Miocene-Early Pliocene, Argentina) Carmona, Noelia Beatriz; Ponce, Juan Jose; Wetzel, Andreas The Discussion of our paper by Martínez is very welcome because it supports our finding that bivalves may colonize wet-interdune settings and thus, trace fossils produced by them may record environmental changes—this is the main point of the contribution by Carmona et al. (2018). However, there are some comments in the Discussion by Martínez about the interpretations proposed in that paper that need to be clarified here.

La sintaxis de los nombres de relación (no) simétrica; The syntax of (non) symmetric relational nouns Mare, María de Los Angeles Este artículo se centra en el análisis de los nombres de relación (no) simétrica desde un enfoque neo-construccionista en el marco de la Morfología Distribuida. Los objetivos de la investigación son tres. En primer lugar, se busca poner en relieve los problemas que suponen estos nombres para las discusiones teóricas semánticas, sintácticas y morfofonológicas. En segundo lugar, se procura retomar discusiones de la literatura sobre el género en español y la relación de esta noción con la simetría (Esteban es hermano de Ana, pero Ana no es hermano de Esteban, sino hermana de Esteban). Finalmente, se intenta avanzar en un análisis de la estructura argumental que dé cuenta de las alternancias que se reconocen en estos nombres (Esteban es hermano de Ana/Esteban y Ana son hermanos) y el rol de la información de número en esa relación. Lo novedoso de esta propuesta es que retoma algunas discusiones clásicas, como las referidas al género y a la predicación, y avanza en soluciones que permiten explicar un conjunto de nombres que constituyen una clase natural. Asimismo, la investigación indaga en discusiones teóricas como la individuación de raíces y la relación entre el componente sintáctico y el componente interpretativo. Finalmente, se avanza en la propuesta de extender la distinción entre elementos relacionales (categorizadores, PLACE) y no relacionales (Sintagmas Determinantes, raíces) al ámbito nominal para explorar cómo se establecen las relaciones de predicación y referencialidad.; The following paper aims to provide an analysis of (non) symmetric relational nouns in Spanish, accounting for their semantic, syntactic and morpho-phonological characteristics. Accordingly, two aspects are revisited: on the one hand, the relationship between gender and symmetry (Esteban es hermano de Ana ‘Esteban is brother of Ana’, but Ana es hermana/*hermano de Esteban ‘Ana is sister/*brother of Esteban’); and, on the other hand, the alternations regarding the argument structure of these nouns (Esteban es hermano de Ana ‘Esteban is brother of Ana’/Esteban y Ana son hermanos ‘Esteban and Ana are brother and sister’). The novelty of this proposal is that it recovers classical discussions, such as the ones related to gender and predication, and sheds light on a group of nouns that conforms a natural class. Since this research is framed in a neoconstructionist approach like Distributed Morphology, it focuses on the discussion about root individuation and the relationship between syntactic derivation and the conceptual component. Last but not least, it extends the distinction between relational (categorizers, PLACE) and non-relational (DDPP and roots) elements into the nominal scope, in order to explore the difference in predication and referential expressions.

Facilitated proton transfer reactions via water autoprotolysis across oil|water interfaces. Spectroelectrochemical analysis Vega Mercado, Franco; Fernández, Ricardo Ariel; Iglesias, Rodrigo Alejandro; Dassie, Sergio Alberto In this paper, we present an integrated experimental-theoretical approach to evaluate the spectroelectrochemical response of the facilitated proton transfer processes via water autoprotolysis at the liquid|liquid (L|L) interface. The aim of these studies is to confirm theoretically and experimentally the pH gradients generated at the L|L interface using coupled electrochemical and spectroscopic techniques. The spectroelectrochemical measurements are performed using a parallel beam configuration, where the light beam passes at grazing incidence over the L|L interface. pH gradients generated by proton transfer assisted by Quinidine via water autoprotolysis are evaluated by the direct measurement of the optical absorption of Thymol Blue, acting as a pH probe. Results obtained are correlated with a numerical model considering all equilibria involved at both phases.

On the lack of correlation between [O III ]/[O II ] and Lyman continuum escape fraction Bassett, R.; Ryan-Weber, E.V.; Cooke, J.; Diaz, Carlos Gonzalo; Nanayakkara, T.; Yuan, T. T.; Spitler, L.R.; Mestric, U.; Garel, T.; Sawicki, Silvana Mabel; Gwyn, S.; Golob, A. We present the first results of our pilot study of eight photometrically selected Lyman-continuum (LyC) emitting galaxy candidates from the COSMOS field and focus on their optical emission line ratios. Observations were performed in the H and K bands using the Multi-Object Spectrometer for Infra-Red Exploration (MOSFIRE) instrument at the Keck Observatory, targeting the [O ii], H β, and [Oiii] emission lines. We find that photometrically selected LyC emitting galaxy candidates have high ionization parameters, based on their high [O iii]/[O ii] ratios (O32), with an average ratio for our sample of 2.5 ± 0.2. Preliminary results of our companion Low-Resolution Imaging Spectrometer (LRIS) observations, targeting LyC and Ly α, show that those galaxies with the largest O32 are typically found to also be Ly α emitters. High O32 galaxies are also found to have tentative non-zero LyC escape fractions (f esc (LyC)) based on u band photometric detections. These results are consistent with samples of highly ionized galaxies, including confirmed LyC emitting galaxies from the literature. We also perform a detailed comparison between the observed emission line ratios and simulated line ratios from density bounded H ii regions modelled using the photoionization code MAPPINGS V. Estimates of f esc (LyC) for our sample fall in the range from 0.0 to 0.23 and suggest possible tension with published correlations between O32 and f esc (LyC), adding weight to dichotomy of arguments in the literature. We highlight the possible effects of clumpy geometry and mergers that may account for such tension.

Los antiguos y los modernos en la larga duración (siglos XVI-XVIII) Vidal, Silvina Paula Este dossier temático aborda, a partir de la discusión de estudios de caso, los modos en que el hombre del Renacimiento forjó una identidad bifronte, ambigua y desgarradora tensión, en tanto hijo de una gloriosa Antigüedad y simultáneamente Moderno, es decir, prueba viviente de algo completamente nuevo, que todavía estaba por venir. Como bien han señalado Paul Hazard y François Hartog, el binomio Antiguos/ Modernos se juega en la temporalidad, en las formas en que los hombres de la temprana modernidad europea percibían el pasado, dando lugar a querellas que expresaban momentos de crisis y conflicto. Las cinco contribuciones reunidas aquí indagan, entre 1500 y 1800, no sólo los vínculos que se establecen entre distintos saberes y conocimientos (especialmente entre razón, fe, autoridad y experiencia), sino también en los lenguajes, las representaciones, los discursos y las prácticas culturales que los actores del período construyeron en un intento por hacer inteligible y llegar a controlar las nuevas realidades geográficas, políticas, religiosas, sociales y económicas que atravesaban sus sociedades y entornos en esos convulsionados siglos, conformando así un entramado complejo de relaciones entre pasado, presente y futuro.

La prensa del Movimiento Nacionalista Revolucionario Tacuara en las mutaciones del nacionalismo argentino; The press of the Tacuara Revolutionary Nacionalist Movement in the mutations of argentine nationalism Campos, Esteban Javier Tacuara del manchón fue la prensa oficial del Movimiento Nacionalista Revolucionario Tacuara (MNRT) liderado por Joe Baxter y José Luis Nell, una fuente documental que hasta el momento no ha sido tratada de manera privilegiada por ninguna investigación. El análisis de los tres números que tuvo la publicación en 1963, permitirá revisar las concepciones del MNRT sobre la revolución, la vanguardia, el peronismo, el tercer mundo, la raza y la clase social. Estas categorías serán comparadas con otras publicaciones del universo tacuarista, como Tacuara. Vocero oficial de la juventud nacionalista, Ofensivay Barricada. La idea que gobierna este trabajo es que Tacuara del manchón puso en palabras una pequeña pero significativa mutación del nacionalismo argentino: el pasaje de la ideología nacional-católica del Movimiento Nacionalista Tacuara, caracterizada por una teología política en clave racialista, a un nacionalismo más secularizado, interesado en la emancipación económica y social del Tercer Mundo.; Tacuara del manchón was the press publication of the Tacuara Revolutionary Nationalist Movement led by Joe Baxter and José Luis Nell, a documentary source that until now has not been studied in a privileged way in any research. The analysis of the three issues published in 1963 will allow to examine MNRT’s understandings of revolution, vanguard, Peronism, third world, race and social class. These categories will be compared to other publications within Tacuara's domain, such as Tacuara. Vocero oficial de la juventud nacionalista, Ofensiva and Barricada. The idea that guides this paper is that Tacuara del manchón put into words a small but significant mutation of Argentine nationalism: the passage from the national-Catholic ideology of the Tacuara Nationalist Movement, characterized by a political theology with a racial orientation, to a more secularized nationalism, interested in the economic and social emancipation of the Third World.

Understanding the transition from water to land: Insights from multi-omic analyses of the perivitelline fluid of apple snail eggs Ip, Jack C.H.; Mu, Huawei; Zhang, Yanjie; Sun, Jin; Heras, Horacio; Chu, Ka Hou; Qiu, Jian-Wen Unlike most of the freshwater gastropod families, the family Ampullariidae includes members that exhibit both underwater and aerial oviposition, making it an ideal model for understanding mechanisms underlying the evolutionary transition from water to land. We applied SDS-PAGE and LC-MS/MS to analyse the proteome of the egg perivitelline fluid (PVF) of Marisa cornuarietis - an aquatic ovipositing ampullariid. Comparison with the reported PVF proteomes of two aerial ovipositing ampullariids (Pomacea canaliculata and P. maculata) showed that the three species all contain several major perivitellins that nourish the embryos. However, M. cornuarietis invests more heavily on immune-related proteins, which might be due to exposure to aquatic pathogens. Interestingly, only the PVF of out-of-water egg laying species have PV2 - a neurotoxin lethal to mice, and a calcium-binding protein which might be involved in the formation of calcareous eggshell. Integrated phylogenetic, evolutionary and gene expressional analyses detected the involvement of gene duplication, positive selection and neofunctionalisation in the formation of several major PVF proteins. Overall, our study provides multiple lines of evidence of adaptive evolution in the PVF proteins, and contributes to a better understanding of how aquatic gastropod ancestors invaded terrestrial habitats. Significance: Aerial egg deposition has evolved in several groups of animals, but except for Vertebrata little is known about the mechanisms underlying this critical evolution process. We compared aquatic and aerial egg laying apple snails to understand the molecular mechanisms enabling such a transition in egg laying habitat. We found that the composition of perivitelline fluid proteomes of underwater and aerial egg depositors was remarkably different, and then gene duplication and positive selection were responsible for the formation of such novel proteins than enabled the evolutionary transition.