Microbiological pollution of intramural air in a school in the city of La Plata and its impact on respiratory conditions Aguilar, Myrian Elizabeth; Brignoles, Pedro Manuel; Viegas Caetano, José; Acosta, Marina Alejandra; Porta, Andrés Osvaldo; Astoreca, Andrea Luciana The effects of indoor air pollution have received increased attention worldwide. Schools are places with a high level of activity and population density of children, where different pollutants from both indoor and outdoor sources may be introduced and persist for a long time. Indoor air quality in school buildings is characterized by various pollutants, such as volatile organic compounds (VOCs), particulate matter (PM2.5 and PM10), fungi and bacteria. In this context, the objective of this study was to describe and characterize the microbial contamination present in the indoor atmosphere of a public school in our city in relation to its impact with the respiratory disorders of those who come to that establishment. The predominant genera of airborne fungi isolated in indoor air of the college were Cladosporium, Epicoccum, Ulocladium, Aspergillus, Fusarium and Penicillium. The predominant bacteria in this work were some species belonging to the genus Pseudomonas and Bacillus, added to Micrococcus luteus, which persisted in the different sampled seasons. Mold and bacteria monitoring shows differences in the found genera/species in the assayed seasons of the year, which is correlated with the variation in environmental conditions. This fact does not seem to be affecting the respiratory physiological parameters of the evaluated children since they are within normal values, according to the international literature. When compared with the international bibliography, they show to be slightly smaller, highlighting the importance of having specific equations to the region under study. In future studies, an attempt will be made to correlate the found microorganisms with the profiles of volatile metabolites organic compounds (MVOC´s) and their effects on the respiratory health of a group of adolescents from La Plata city.

Ultrasonografía del aparato urogenital Batista, Pablo Rodrigo La ultrasonografía del aparato urinario se ha vuelto una técnica de uso rutinario para el diagnóstico de diversas enfermedades renales y vesicales. Si bien no brinda información acerca de los cambios funcionales, la evaluación de las modificaciones estructurales permite una aproximación diagnóstica certera, la realización de un seguimiento de la evolución del paciente y una estimación del pronóstico.

Chemical structure and rheological studies of arabinoglucuronoxylans from the Cercidium praecox exudate brea gum Sznaider, Frank; Rojas, Ana Maria Luisa; Stortz, Carlos Arturo; Navarro, Diego Alberto The structure of the arabinoglucuronoxylans from brea gum was elucidated through an chemical and NMR spectroscopical analysis. They are composed of xylose, arabinose, glucuronic acid and 4-O-methylglucuronic acid in a molar ratio 1:0.44:0.16:0.22. The structure consists of a central chain of (1→4)-β-D-xylopyranose of which ca.70% are susbstituted in C2 with single stubs of others sugars (β-D-Xylp, α-D-GlcpA and 4-O-Me-α-D-GlcpA), with disaccharides (α-L-Arap-(1→2)-4-O-Me-α-D-GlcpA-(1→, α-L-Arap-(1→2)-α-D-GlcpA-(1→, β-L-Araf-(1→3)-α-L-Araf-(1→ and α-L-Araf-(1→3)-α-L-Araf-(1→5), and possibly with trisaccharides of xylose. The determination of the location of the acetyl groups and their quantification in these arabinoglucuronoxylans has been achieved for the first time. Brea gum presents a higher thickening effect than gum arabic in 5% aqueous solution, demonstrating its potential usefulness for food and pharmaceutical applications.

Potenciar la graduación: acciones y voces desde la Maestría en Ciencias Sociales Basconzuelo, Celia Cristina; Elisondo, Romina Cecilia Este capítulo de libro refleja las políticas adoptadas en la Maestría en Ciencias Sociales, respecto de potenciar los estudios de posgraduación. Se partió de un diagnóstico. El análisis de las trayectorias académicas de los estudiantes en la carrera indicaba que muchos de ellos lograban culminar los cursos obligatorios, los seminarios optativos y los talles de tesis y, sin embargo, no conseguían presentar sus trabajos finales de maestría. Ante esta situación, las autoridades de la carrera decidieron generar una serie de acciones orientadas a promover el desarrollo de los procesos de elaboración de tesis. Nos interesa en el presente capítulo compartir dichas acciones. Asimismo, es nuestro interés recuperar voces graduados respecto de la carrera y las potenciales contribuciones de los conocimientos construidos en sus trayectorias académicas y profesionales. Los datos presentados en el capítulo emergen del análisis de documentos que condensan un conjunto de acciones desarrolladas en la Maestría. También analizamos datos emergentes de cuestionarios abiertos y entrevistas realizadas a graduados de la carrera.

Class I TCP transcription factors activate the SAUR63 gene subfamily in gibberellin-dependent stamen filament elongation Gastaldi, Victoria; Lucero, Leandro Exequiel; Ferrero, Lucia; Ariel, Federico Damian; Gonzalez, Daniel Hector In autogamous plants like Arabidopsis (Arabidopsis thaliana), stamen filament elongation must be finely regulated to ensure that anthers reach the pistil at the correct developmental stage. In this work, we studied the roles of Arabidopsis TEOSINTE BRANCHED1, CYCLOIDEA, PCF15 (TCP15), and related class-I TCP transcription factors in stamen filament elongation. Plants with decreased expression of class-I TCPs and plants that express a fusion of TCP15 to a repressor domain (pTCP15::TCP15-EAR) had shorter stamens, indicating that class-I TCPs stimulate filament growth. These plants also showed reduced expression of several SMALL AUXIN UP RNA (SAUR)63 subfamily genes, which contain TCP target motifs in their promoters. Mutational analysis indicated that the TCP target motif in the SAUR63 promoter is required for expression of SAUR63 in stamen filaments. Moreover, TCP15 directly binds to the SAUR63 promoter region that contains the TCP target motif in vivo, highlighting the role of the TCPs in this process. Class-I TCPs are also required for the induction of SAUR63 subfamily genes by gibberellins (GAs). In addition, overexpression of SAUR63 restores filament growth in pTCP15::TCP15-EAR plants, whereas overexpression of TCP15 rescues the short stamen phenotype of GA-deficient plants. The results indicate that TCP15 and related class-I TCPs modulate GA-dependent stamen filament elongation by direct activation of SAUR63 subfamily genes through conserved target sites in their promoters. This work provides insight into GA-dependent stamen filament elongation.

Lotus Spp.: A foreigner that came to stay forever: Economic and environmental changes caused by its naturalization in the Salado River Basin (Argentina) Nieva, Amira Susana del Valle; Ruiz, Oscar Adolfo The Flooding Pampas in Buenos Aires Province is Argentina’s is one of cattle-raising areas. Climatic, topographic and edaphic conditions limit its potential in this area for growing crops such as soybean and wheat. The introduction of L. tenuis in the Flooding Pampas area triggered research based on its ability to tolerate the abiotic stresses that characterize the region and on its role in the improvement of the quality of forage resources. Along with research on abiotic stress tolerance, productive strategies have been developed to enhance the establishment of L. tenuis grassland and beef production. Research on Lotus spp. in the Flooding Pampas has therefore studied not only the biotechnological development and evaluation of new plant resources, but also the accompanying plant diversity, soil microorganisms and symbionts and their impact on environmental dynamics and sustainability. Based on this research, productive strategies have been designed, including continuous evaluation of the impact of cattle production on vulnerable ecosystems. In addition, basic and applied research on grasslands have been combined in order to respond to the environmental impact of the introduction and use of Lotus in these particular ecosystems.

Las macrauchenias de Kamac Mayu Gelfo, Javier Nicolás En el yacimiento excepcional de Kamac Mayu, una importante concentración de fósiles se encuentra representada en niveles fluviales conformados por gravas arenosas y arenas, depositados sobre canales cársticos de la Formación Chiu Chiu (Chong Diaz y Jensen, 2004). La diversidad faunística conservada comprende restos de Aves Rheidae y mamíferos representados por el équido Hippidion saldiasi, Xenarhtra indeterminados, camélidos como Lama gracilis (Camelidae, Mammalia) (López y Labarca 2005, Alberdi et al. 2007) y una profusa cantidad de restos de Macrauchenia patachonica indicando la presencia de varios individuos (Gelfo et al., 2008).

El abordaje de territorios vulnerables afectados por inundaciones en el Gran La Plata a partir de la utilización del método Stlocus Frediani, Julieta Constanza; Cortizo, Daniela Ethel; Rodriguez Tarducci, Rocio El presente trabajo aborda la aplicación del método Stlocus (Bozzano y Resa, 2009) en dos áreas de estudio dentro del Gran La Plata, para lograr la definición y el reconocimiento de lugares, en el marco del Proyecto PIO-UNLP (2014-2016) “Estrategias para la Gestión Integral del Territorio. Vulnerabilidades y Procesos de Intervención y Transformación con Inteligencia Territorial. Métodos y técnicas científicas ambientales, sociales y espaciales: Dos casos en el Gran La Plata”, tendiente a aportar al objetivo general del mencionado trabajo consistente en “proponer y ejecutar a partir de los aportes concomitantes de ciudadanos, científicos, políticos y empresarios, mediante pensamiento, producción y acción con base científica, resultados relacionados con la gestión sostenible e integral de lugares −o micro-territorios− específicos a la vez que afectados por las inundaciones del 2 de abril de 2013, con rasgos de vulnerabilidad social y/o ambiental estructurales, previos a la inundación, poniendo énfasis en soluciones tecnológicas a problemáticas ambientales con alto grado de participación comunitaria e institucional”.

"Dying well" in Latin America: Christian martyrdom, religion and politics Catoggio, Maria Soledad On the basis of the distinction between a “theological” treatment of the figure of the martyr and the historiography of martyr narratives in Latin America, this paper analyzes the variations of the martyr figure considering its emergence in different religious and political discourses from colonial times to the present. This review shows the historically controversial position of this figure, on the border between religion and politics. Its threshold position also varies depending on the viewpoint from which the martyr narrative is enunciated, the place and specific time in history. Thus, far from converging on a univocal sense, the figure multiplies its senses, even leading to opposing meanings.

Fengycins from bacillus amyloliquefaciens MEP218 exhibit antibacterial activity by producing alterations on the cell surface of the pathogens Xanthomonas axonopodis pv. vesicatoria and Pseudomonas aeruginosa PA01 Medeot, Daniela Beatriz; Fernandez, Maricruz; Morales, Gustavo Marcelo; Jofré, Edgardo Bacillus amyloliquefaciens MEP218 is an autochthonous bacterial isolate with antibacterial and antifungal activities against a wide range of phytopathogenic microorganisms. Cyclic lipopeptides (CLP), particularly fengycins, produced by this bacterium; are the main antimicrobial compounds responsible for the growth inhibition of phytopathogens. In this work, the CLP fraction containing fengycins with antibacterial activity was characterized by LC-ESI-MS/MS. In addition, the antibacterial activity of these fengycins was evaluated on the pathogens Xanthomonas axonopodis pv. vesicatoria (Xav), a plant pathogen causing the bacterial spot disease, and Pseudomonas aeruginosa PA01, an opportunistic human pathogen. In vitro inhibition assays showed bactericidal effects on Xav and PA01. Atomic force microscopy images revealed dramatic alterations in the bacterial surface topography in response to fengycins exposure. Cell damage was evidenced by a decrease in bacterial cell heights and the loss of intracellular content measured by potassium efflux assays. Furthermore, the viability of MRC-5 human normal lung fibroblasts was not affected by the treatment with fengycins. This study shows in vivo evidence on the less-known properties of fengycins as antibacterial molecules and leaves open the possibility of using this CLP as a novel antibiotic.

Degradation of a series of fluorinated acrylates and methacrylates initiated by OH radicals at different temperatures Lugo Garcia, Pedro Luis; Rivela Fretes, Cynthia Beatriz; Gibilisco, Rodrigo Gastón; Salgado, S.; Wiesen, P.; Teruel, Mariano Andres; Blanco, Maria Belen Rate coefficients for the gas-phase reactions of OH radicals with a series of fluorinated acrylates and methacrylates: 2,2,2-trifluoroethylmethacrylate (k1), 1,1,1,3,3,3-hexafluoroisopropylacrylate (k2), 1,1,1,3,3,3-hexafluoroisopropylmethacrylate (k3), and 2,2,2-trifluoroethylacrylate (k4) have been measured for the first time as a function of temperature in the range 290-308 K. The kinetic data obtained were used to derive the following Arrhenius expressions (in units of cm3 per molecule per s): k1 = (2.13 ± 0.68) × 10-18 exp[(4745 ± 206)/T], k2 = (8.72 ± 0.68) × 10-15 exp[(2166 ± 205)/T], k3 = (6.30 ± 0.51) × 10-17 exp[(3721 ± 153)/T] and k4 = (3.93 ± 0.43) × 10-16 exp[(3140 ± 129)/T]. The experiments were performed at normal atmospheric pressure in synthetic air using a 1080 L photoreactor and coupled with FTIR analysis to monitor the decay of the substances of interest and the reference compounds. The obtained negative temperature dependencies are in agreement with a mechanism implying an initial addition of the OH radical to the double bond. Atmospheric implications are discussed with reference to the rate coefficients obtained as a function of the temperature.

Skepticism and Clandestine Literature: Doutes des Pyrrhoniens Bahr, Fernando Anibal The latest studies on clandestine philosophical literature reveal the importance of this corpus to understand how skeptical tropes and lexicon were modified during the Modern Age. We will try to show it by means of an anonymous text written surely after 1711 and entitled Doutes des pyrrhoniens. First, we will present the manuscript in its most important arguments; second, we will concentrate in the eighth chapter and specially in the relationship between religion and politics; finally, we will take into account a differentiation that the author establishes between "moderate pyrrhonism" and "furious pyrrhonism" to consider its importance. Our main argument will be that the difference between these two forms of pyrrhonism is political, not epistemological. Indeed, both forms coincide in that religion is a fact of power that has nothing to do with an alleged divine revelation. The question is no longer about the truth of religion, but about whether or not it is convenient to abandon it as a regulatory component of society.

Enhancing Water Resistance, Tensile Properties and UV-Shielding Capacity of Bovine Gelatin Films Modified with Epoxidized Soybean Oil and Glyoxal during Processing González Granados, María Lucía; Martucci, Josefa Fabiana; Ruseckaite, Roxana Alejandra The present work is focused on the use of epoxidized soybean oil (ESO, 10 wt%) as hydrophobizing additive, glycerol as plasticizer (Gly, 30 wt.%),and glyoxal (GLX, 2 wt%) as low-toxicity cross-linking agent to endow bovine gelatin (BGe) films with improved moisture-related and UV-light barrier properties. Therefore, we aimed to investigate the effect of such modifications on the physicomechanical and structural properties of the ob-tained gelatin films. Modification was produced during intensive mixing and hot-press molding as a more environmentally sound and less time-con-suming processing method. ESO improved moderately the moisture content (MC) and water vapor permeability (WVP) of BGe film by 19% and 29%, respectively, but the total solubility of BGe-10ESO film evidenced the inability of ESO to induce covalent cross-linking. When GLX was add-ed to the BGe-10ESO formulation, several target film properties were upgraded; the film kept about 78% of its weight after 24 h of soaking in water, WVP improved by 56.8%, the tensile strength (TS) increased 48% while the stretching ability dropped in about 41%. The significant reduction in available free amino groups, from 100% (control) up to 21.9%, together with the increased color from light yellow to light orange confirmed that amino groups in BGe were involved in imine-based cross-linked structure when treated with glyoxal. Further, the high blocking ability of BGe-10ESO-2GLX film against UV-radiation may be useful in delaying UV-induced lipid oxidation when it is placed in contact with foods.

Vicia: a green bridge to clean up polluted environments Ibañez, Sabrina Guadalupe; Medina, Maria Ines; Agostini, Elizabeth Vicia species, commonly known as vetches, include legume plants which nowadays can be found in many countries around the world. Their use to improve soil health and productivity is crucial in management schemes that make sustainable agriculture possible, but they can also play a part in the phytoremediation of polluted environments. Furthermore, they harbor a large community of rhizospheric microorganisms, such as biodegradative bacteria and plant growth–promoting rhizobacteria, which can help to increase phytoremediation efficiency. Their mutualistic association with Rhizobium sp. has also been proposed as an attractive bioremediation tool. Thus, Vicia species could make a remarkable difference in the ecological restoration of polluted soils, thanks to their dual role as cover crops and phytoremediator plants. This mini-review discusses recent advances in the use of Vicia. Challenges and opportunities connect with the application of these species will also be revised, as well as aspects that remain to be explored.

El registro arqueologico de valles de altitud intermedia en la cuenca del Río Diamante; The archaeological record of mid-altitude valleys in the diamante River Basin Otaola, Clara; Giardina, Miguel Angel; Franchetti, Fernando Ricardo En este trabajo se presentan los resultados del análisis de cuatro sitios arqueológicos localizados en los valles cordilleranos de altitud intermedia del río Diamante: El Perdido 1, El Perdido 4, El Perdido 5 y HI95.Se utiliza la información de materiales arqueológicos recolectados en superficie y de sondeos estratigráficos realizados en cada uno de estos sitos. El objetivo del trabajo es caracterizar el tipo de asentamiento y evaluar si hubo un uso diferencial de este espacio. Se busca, a su vez, evaluar cambios a lo largo del tiempo y entre sitios analizados. En este sentido, se encontraron diferencias en la intensidad de uso de cada uno de estos sitios. El Perdido 1 se presenta como el sitio con mayor intensidad de uso y mayor diversidad artefactual, por el contrario, HI95 es el que posee menor intensidad de uso. Para evaluar las diferencias en la intensidad de uso de cada sitio se consideró la disponibilidad de materias primas líticas y la localización de las mismas. En cuanto a las diferencias temporales, se ha registrado el uso de materias primas locales durante la primera mitad del Holoceno tardío y la incorporación de materiales alóctonos, tales como obsidianas y cerámicas con estilos no locales, a partir de los 2000 años AP. Se concluye que en este sector de la cuenca habrían predominado cazadores-recolectores móviles con características muy similares a los que habitaron en la cuenca de los ríos Salado y Atuel.; In this paper, we present the analysis of material from four archaeological sites located in the mid-altitude valleys in the Diamante River Basin: El Perdido 1, El Perdido 4, El Perdido 5, and HI95. Archaeological information from the surface and stratified excavations is presented. The objective of this paper is to characterize the settlements and subsistence patterns of the people who inhabited this part of the basin as well as changes over time. In this regard, there are differences in the intensity of the occupations. El Perdido 1 has the highest occupational intensity and artefactual diversity. HI95 has the lowest area-density index, showing a less intense use. These differences have been analyzed considering lithic raw material availability and their locations. Regarding temporal trends, the use of local materials is documented for the first half of the late Holocene, and non-local materials, such as obsidian and non-local ceramic styles, starting two thousand years ago. It is concluded that these sites were occupied by mobile hunter-gatherers with very similar characteristics to those living in neighboring river basins.

El sentido de la educación desde lo humano: Apuntes a partir de Martha Nussbaum y Francisco Ruiz Sánchez; The Meaning of Education From a Human Perspective: Remarks From Martha Nussbaum and Francisco Ruiz Sánchez; O sentido da educação proveniente do humano: notas de Martha Nussbaum e Francisco Ruiz Sánchez Di Marco, María Elisa En el presente trabajo, se realiza un análisis del sentido de la educación desde una mirada humana a partir del pensamiento de Martha Nusbaum y Francisco Ruiz Sánchez, en el que se observan algunos puntos en común y algunas diferencias entre los planteos de estas personas autoras. En efecto, en contraposición de las posturas mercantilistas y a favor de una apuesta antropológica, sus estudios insisten en la necesidad de una educación a la medida del ser humano. El estudio hermenéutico que se propone aquí se centra en la teoría de las capacidades de Nussbaum (2007, 2012) y la vinculación de esta con el concepto de dignidad humana. Por otra parte, se abordan conceptos fundamentales de la visión antropológica de Ruiz Sánchez (1962a, 1962b, 1978a) como el de la perfectibilidad y la falibilidad, además de otros aspectos que promueven la educación como búsqueda de la plenitud humana. Luego de realizar este análisis, se arriba a la conclusión de que existen numerosos aspectos en común entre las dos visiones revisadas, sin dejar de advertir que también poseen diferencias importantes. No obstante, ambas teorizaciones posibilitan comprender la educación desde una perspectiva humanista, es decir, como un fin en sí misma, pues dota al ser humano de dignidad y plenitud.; This paper analyzes the meaning of education from a humanist perspective developed by Martha Nussbaum and Francisco Ruiz Sánchez. There are some common points and some important differences between the proposals of these authors. In fact, in contrast to the mercantilist perspective and in favor of an anthropological position, both authors insist on the need for a more humanist education, at the measure of the human being. The hermeneutic study proposed here focuses on Nussbaum’s theory of capabilities (2007, 2012) and its links with the concept of human dignity. On the other hand, this paper presents basic concepts about the anthropological view of Ruiz Sánchez (1962a, 1962b, 1978a) such as perfectibility and fallibility, as well as other aspects that promote education as a search for human fulfillment. After carrying out this analysis, we conclude that there are many aspects in common between the two authors reviewed, but also important differences. However, both theorizations make it possible to understand education from a humanist perspective, that is, as an end in itself, because it endows the human being with dignity and fullness.; Neste artigo, é feita uma análise do sentido da educação a partir de uma perspectiva humana, com base nos pensamentos de Martha Nusbaum e Francisco Ruiz Sánchez, nos quais são observados alguns pontos em comum e algumas diferenças entre as abordagens dessas pessoas como autoras. De fato, em contraste com as posições mercantilistas e a favor de um compromisso antropológico, seus estudos insistem na necessidade de uma educação adaptada ao ser humano. O estudo hermenêutico proposto aqui enfoca a teoria das capacidades de Nussbaum (2007, 2012) e sua ligação com o conceito de dignidade humana. Por outro lado, são abordados conceitos fundamentais da visão antropológica de Ruiz Sánchez (1962a, 1962b, 1978a), como o de perfeição e falibilidade, além de outros aspectos que promovem a educação como busca da realização humana. Após realizar essa análise, a conclusão encontrada é que existem inúmeros aspectos em comum entre as duas visões revisadas, observando que elas também apresentam diferenças importantes. No entanto, ambas teorizações tornam possível entender a educação sob uma perspectiva humanística, isto é, como um fim em si mesmo, porque confere dignidade e plenitude ao ser humano.

La igualdad en John Rawls y la herencia de riquezas en Argentina Berrotarán, Alejandro En la obra de John Rawls, la herencia de riquezas se encuentra justificada en la medida en que beneficie a los más desaventajados. Pero esta justificación se encuentra supeditada a la satisfacción de las exigencias prioritarias de igualdad que los demás principios de justicia imponen sobre esta institución en particular. De esta manera, el objetivo central del trabajo es responder a la siguiente pregunta: ¿la regulación argentina de la herencia de riquezas satisface las exigencias igualitarias de la teoría de justicia de Rawls? Para esto empezaré por reconstruir la teoría liberal igualitaria en la que se encuadra este autor. Posteriormente, abordaré la concepción de igualdad rawlsiana para ver las exigencias específicas que este valor establece sobre la regulación de la herencia. Luego, analizaré la actual legislación argentina que regula esta institución. Finalmente, a partir de los marcadores teóricos abordados, evaluaré la normativa nacional y realizaré algunas consideraciones para responder a la pregunta central planteada.

From Centaurs to comets: 40 years Peixinho, Nuno; Thirouin, Audrey; Tegler, Stephen C.; Di Sisto, Romina Paula; Delsanti, Audrey; Gilbert Lepoutre, Aurelie; Bauer, James G. In 1977, while Apple II and Atari computers were being sold, a tiny dot was observed in an inconvenient orbit. The minor body 1977 UB, to be named (2060) Chiron, with an orbit between Saturn and Uranus, became the rst Centaur, a new class of minor bodies orbiting roughly between Jupiter and Neptune. The observed overabundance of short-period comets lead to the downfall of the Oort Cloud as exclusive source of comets and to the rise of the need for a Trans-Neptunian comet belt. Centaurs were rapidly seen as the transition phase between Kuiper Belt Objects (KBOs), also known as Trans-Neptunian Objects (TNOs) and the Jupiter-Family Comets (JFCs). Since then, a lot more has been discovered about Centaurs: they can have cometary activity and outbursts, satellites, and even rings. Over the past four decades since the discovery of the rst Centaur, rotation periods, surface colors, reflectivity spectra and albedoshave been measured and analyzed. However, despite such a large number of studies and complementary techniques, the Centaur population remains a mystery as they are in so many ways dierent from the TNOs and even more so from the JFCs.

La competitividad como valor social: la gestión pública de turismo en la Argentina del siglo XXI Mas, Fernando Francisco La competitividad, luego del proceso de racionalización que tuvo lugar entre los años cincuenta y setenta, logró divulgarse y ponerse en evidencia con vigor recién desde la década del ochenta. En esta década, en virtud de varios autores, pero sobre todo de Porter, logró transmutar en un valor y en un principio que ofrece el marco general de la ética y de la racionalidad empresarial contemporánea. La racionalización devino en ética, y en racionalidad, a raíz de la excesiva divulgación de los métodos de gestión (estratégica) más efectivos para vencer a un competidor. La noción, como sus matrices de cálculo (técnicas racionales) y sugerencias asociadas a ésta, efectivamente transmutó en un valor social. De hecho, desbordó el espacio confinado netamente al sector privado, afectando a otros ámbitos, como por ejemplo, a la misma gestión pública estatal. En este capitulo nos encargaremos de visibilizar esta situación analizando los planes federales de turismo publicados en Argentina en lo que va del siglo XXI.

Angiotensin-II-Evoked Ca2+ Entry in Murine Cardiac Fibroblasts Does Not Depend on TRPC Channels Camacho Londoño, Juan E.; Marx, André; Kraft, Axel E.; Schürger, Alexander; Richter, Christin; Dietrich, Alexander; Lipp, Peter; Birnbaumer, Lutz; Freichel, Marc TRPC proteins form cation conducting channels regulated by different stimuli and are regulators of the cellular calcium homeostasis. TRPC are expressed in cardiac cells including cardiac fibroblasts (CFs) and have been implicated in the development of pathological cardiac remodeling including fibrosis. Using Ca2+ imaging and several compound TRPC knockout mouse lines we analyzed the involvement of TRPC proteins for the angiotensin II (AngII)-induced changes in Ca2+ homeostasis in CFs isolated from adult mice. Using qPCR we detected transcripts of all Trpc genes in CFs; Trpc1, Trpc3 and Trpc4 being the most abundant ones. We show that the AngII-induced Ca2+ entry but also Ca2+ release from intracellular stores are critically dependent on the density of CFs in culture and are inversely correlated with the expression of the myofibroblast marker α-smooth muscle actin. Our Ca2+ measurements depict that the AngII- and thrombin-induced Ca2+ transients, and the AngII-induced Ca2+ entry and Ca2+ release are not affected in CFs isolated from mice lacking all seven TRPC proteins (TRPC-hepta KO) compared to control cells. However, pre-incubation with GSK7975A (10 µM), which sufficiently inhibits CRAC channels in other cells, abolished AngII-induced Ca2+ entry. Consequently, we conclude the dispensability of the TRPC channels for the acute neurohumoral Ca2+ signaling evoked by AngII in isolated CFs and suggest the contribution of members of the Orai channel family as molecular constituents responsible for this pathophysiologically important Ca2+ entry pathway.