Sindicador de canales de noticias
Transitional photoperiod induces a mania‐like behavior in male mice
Rodrigues Barbosa, Mayara; Lopes Costa, Ellyda Fernanda; Gomes Coimbra, Daniel; Braga Cavalcante Pinto, Vinícius; Leite Goes Gitaí, Daniel; Duzzioni, Marcelo; Crespo, Manuel Tomas; Golombek, Diego Andrés; Chiesa, Juan José; Agostino, Patricia Verónica; Gomes de Andrade, Tiago
This study aimed to investigate the behavioral responses and circadianrhythms of mice to both rapid and gradual increases in photoperiod, mimickingthe transition from winter to summer, which is associated with a heightenedprevalence of hospitalizations for mania and suicidal behavior.Behavioral tests were performed in C57BL/6 male mice exposed to a transitionalphotoperiod, from short to long durations. To determine if circadianrhythms are affected, we measured spontaneous locomotor activity and bodytemperature. Mice exhibited heightened exploratory and risk-taking behaviorscompared with equatorial and static long (16:8 h of light–dark cycle for severaldays) groups. These behaviors were prevented by lithium. Spontaneous locomotoractivity and body temperature rhythms persisted and were effectivelysynchronized; however, the relative amplitude of activity and interdaily stabilitywere diminished. Additionally, the animals displayed increased activityduring the light phase. Photoperiodic transition modulates behavior and circadianrhythms, mirroring certain features observed in bipolar disorder patients.This study introduces an animal model for investigating mania-like behaviorinduced by photoperiodic changes, offering potential insights for suicide preventionstrategies and the management of mood disorders.
Seasonal changes and energy allocation in the edible snail Zidona dufresnei (Caenogastropoda, Volutidae)
Seasonal changes and energy allocation in the edible snail Zidona dufresnei (Caenogastropoda, Volutidae)
Giménez, Juliana; Ojeda, Mariel Adriana; Boy, Claudia Clementina
Energy acquisition and allocation between the somatic compartments are essential for the ecological success of an organism. The seasonal pattern of energy allocation among the diferent organs was evaluated in the snail Zidona dufresnei. The somatic indexes, energy density and energy content of the gonads, digestive gland, foot, and secondary sexual glands of Z. dusfresnei. The lowest foot index (FI), energy density and energy content were found in winter. The high digestive gland index (DI) and energy content (ECDIG) observed in spring likely coincide with the peak of productivity. The male’s gonads did not show seasonal energetic variation, although the gonadosomatic index (GI) of males was lower in summer than in other months. The mass and energy content of the albumen and capsule glands exhibited a decreasing pattern from spring to winter. The foot is suggested as the main energy reservoir in Z. dufresnei, the digestive gland as an energy reservoir that contributes to the gonadal maturation, whereas the energy of the capsule and the albumen glands contributes to the spawning. This study highlights seasonal variations in the energy content of the foot (edible compartment) and the signifcance of secondary sexual glands in understanding the bioenergetics of Z. dusfresnei and ofspring ftness. These fndings should be taken into consideration in fsheries management decisions for this ecologically and commercially important species.
Assessment of nitrogen dioxide concentrations and main sources in a medium-sized city in Latin America using geographic information systems
Assessment of nitrogen dioxide concentrations and main sources in a medium-sized city in Latin America using geographic information systems
Berkovic, Andrea Mariela; Stadler, Carla Sofía; Fusé, Victoria Susana; Picone, Natasha; Ibarlucía, Daniela Giselle; Juliarena, María Paula
Studying atmospheric nitrogen dioxide (NO2) concentrations in urban areas is crucial for assessing air quality and caring for the population’s health; however, the background of studies on this gas in medium-sized cities is scarce, highlighting a significant knowledge gap in the field, especially in Latin America. This study aims to assess the levels of NO2 concentration in a medium-sized city in Argentina using passive samplers over six months. The Geographic Information Systems (GIS) was employed to analyse the spatial distribution of gas concentration using the Inverse Distance Weighting (IDW) interpolation method. Additionally, potential emission sources were evaluated by applying exploratory regression analysis. The study found that atmospheric concentrations of NO2 generally exceeded the values established by the World Health Organization (WHO) of 10 µg/m3. The highest mean concentration (30.4 µg/m3) was determined near a national route and industrial areas. Correlation analysis between the NO2 concentration at each site and possible emission sources suggests that industries are the main sources of this gas.
Actitudes, prácticas y desempeño escolar en estratos vulnerables: Brasil y Argentina comparados
Actitudes, prácticas y desempeño escolar en estratos vulnerables: Brasil y Argentina comparados; Attitudes, Practices, and School Performance in Vulnerable Strata: A Comparison between Brazil and Argentina; Atitudes, práticas e desempenho escolar em estratos vulneráveis: Brasil e Argentina comparados
Miguez, Daniel Pedro
Desde la década de 1960 en adelante una extensa serie de estudios ha puesto en duda la capacidad de la escuela para igualar los niveles de aprendizaje entre estudiantes de distinto origen social. Pese a la diversidad de programas y políticas que han buscado igualar las diferencias, investigaciones llevadas a cabo de distintas maneras y en diversos contextos han encontrado una y otra vez que las diferencias no solo persisten, sino que se reproducen en la escuela. En este artículo abordamos el problema desde una óptica ligeramente diferente. Nuestro objetivo es identificar algunas de las prácticas y actitudes de estudiantes y docentes que se asocian a las diferencias en el desempeño escolar entre estudiantes de menor nivel económico social en Brasil y Argentina. Consecuentemente, no buscamos comprender los mecanismos que reproducen las diferencias entre estratos, sino aquellos que generan mayores logros relativos en los estudiantes con menos recursos. Con este propósito realizamos un análisis comparativo de los resultados de las pruebas PISA del 2018 mediante modelos estadísticos multivariados. Este análisis mostró que ciertos factores, como la empatía docente, la motivación y perseverancia estudiantil o las modalidades de estudio se asocian al desempeño. Pero también puso en evidencia que la magnitud de la asociación varía con el contexto. Estos resultados resultan un aporte al diseño de políticas educativas que procuren incrementar el desempeño en estratos socioeconómicamente desfavorecidos, indicando algunas de las condiciones que pueden favorecerlo, pero advirtiendo también sobre las dificultades de extrapolar resultados entre contextos de manera acrítica.; Since the 1960s, a vast array of studies has questioned the ability of schools to equalize learning levels among students from different social backgrounds. Despite various programs and policies aimed at reducing these disparities, research in diverse contexts has repeatedly found that not only do these differences persist, but they are also reproduced within the school system. This article approaches the issue from a slightly different perspective: its objective is to identify practices and attitudes of students and teachers that are associated with differences in school performance among students from lower socioeconomic backgrounds in Brazil and Argentina. The focus is not on understanding the mechanisms that perpetuate differences between social strata, but rather on identifying those that lead to higher relative achievements among students with fewer resources. A comparative analysis of the 2018 PISA test results was conducted using multivariate statistical models. This analysis revealed that certain factors, such as teacher empathy, student motivation and perseverance, and study methods, are associated with academic performance. However, it also showed that the strength of these associations varies depending on the context. These findings contribute to the design of educational policies aimed at improving performance in disadvantaged groups by highlighting conditions that may foster better outcomes, while also cautioning against uncritically extrapolating results across different contexts.; Desde a década de 1960, uma série de pesquisas tem questionado a capacidade das escolas para igualar os níveis de aprendizagem entre alunos de diferentes origens sociais. Apesar da diversidade de programas e políticas que buscaram equalizar as diferenças, pesquisas em diversos contextos constataram repetidamente que as diferenças não apenas persistem, mas são reproduzidas nas escolas. Este artigo aborda o problema de uma perspectiva um pouco diferente: seu objetivo é identificar práticas e atitudes de estudantes e docentes associadas a diferenças no desempenho escolar entre estudantes de condição social e econômica mais baixa no Brasil e na Argentina. O objetivo não é entender os mecanismos que reproduzem as diferenças entre os estratos, mas sim aqueles que geram maior desempenho relativo entre os estudantes com menos recursos. Para isso, foi realizada uma análise comparativa dos resultados do teste PISA de 2018 por meio de modelos estatísticos multivariados. Essa análise mostrou que determinados fatores, como a empatia docente, a motivação e perseverança estudantil ou as modalidades de estudo, estão associados ao desempenho. Mas também mostrou que a magnitude da associação varia de acordo com o contexto. Esses resultados contribuem para a elaboração de políticas educacionais que buscam aumentar o desempenho em estratos desfavorecidos, indicando algumas condições que podem favorecê-lo, mas também alertando sobre as dificuldades de extrapolar resultados entre contextos de forma acrítica.
Low similarity between parasite communities of ten sympatric carangid species
Low similarity between parasite communities of ten sympatric carangid species
Osuna Cabanillas, Juan M.; Marín Enríquez, Emigdio; Martínez Falcón, Ana P.; Timi, Juan Tomas; Morales Serna, Francisco N.
Host phylogeny and ecological convergence are two factors thought to influence the structure of parasite communities. The aims of this study were to determine the diversity of metazoan parasites of 10 sympatric fish species of the family Carangidae from the southeastern Gulf of California, and to analyze their similarity at infracommunity and component community levels, in order to determine if the host species, particularly those congeneric with similar ecological characteristics, exhibit similar assemblages of parasites. In total, 874 fish specimens were examined and 40 parasite species were identified. The component community was composed by 21 parasite species in Caranx caninus, 20 in C. caballus, 11 in C. vinctus, five in Chloroscombrus orqueta, four inCarangoides otrynter, seven in Hemicaranx leucurus, eight in Selene brevoortii, 14 in S. peruviana, and 11 in Trachinotus rhodopus. The metazoan parasite communities of C. vinctus, Ch. orqueta, H. leucurus, and S. brevoortii are reported here for the first time. The parasite communities of the remaining six carangid species have been reported from regions other than the Gulf of California. All fish species differed significantly regarding the diversity of their parasite infracommunities. This possibly is due to different patterns of habitat use among fish species, and because of the differential host specificity among parasite taxa. Nonetheless, when the analysis was restrictedto common parasite species, some fish showed similar parasite infracommunities, particularly congeners of the genus Selene as well as C. caballus and C. vinctus. The component communities of species of Selene were highly similar (>65%), but the three species of Caranx were not. This result supports the hypothesis that congeneric fish species with similar ecological filters harbor similar parasite communities. However, the difference observed between C. caninus and C. caballus suggests that these species, despite being evolutionary and ecologically related, have different physiological or immunological characteristics (compatibility filters) that may result indifferent parasite communities.
Enantioseparation of pesticides by gas chromatography: Measurement of association constants enantiomer-chiral selector
Enantioseparation of pesticides by gas chromatography: Measurement of association constants enantiomer-chiral selector
Frickel Critto, Emilia Josefina; Prince, Daiana Lorena; Lancioni, Carlina; Castells, Cecilia Beatriz Marta
In this study, the association constants of sixteen pesticides with the chiral selector octakis(6-O-tert-butyldimethylsilyl-2,3-di-O-acetyl)-γ-cyclodextrin were determined. The procedure only involved a few experimental measurements; namely, gas hold-up time and retention time of pesticides in capillary columns, as well as column phase ratio at each temperature condition. Fundamental equations of gas-liquid chromatography were used to estimate association constants. Two sets of columns containing different concentrations of the mentioned chiral selector dissolved in (14 %-cyanopropyl-phenyl)-86 %-methyl-polysiloxane were used. One set included capillary columns without any chemical treatment and the other group included columns that were crosslinked. The systematic comparison between both groups indicated a deleterious effect of the crosslinking on enantioselectivity.Our main objective is to promote the use of gas chromatography for the analysis of volatile and semi-volatile chiral pesticides. Thus, we proposed a simple methodology, based only on chromatographic measurements, to obtain information about the enantiorecognition ability of a particular chiral selector constituting the stationary phase and the influence of the selected polymer on the selectivity experimentally obtained.
Corrosion protection and antibacterial performance of a chitosan/salicylate coating electrogenerated on a magnesium alloy
Corrosion protection and antibacterial performance of a chitosan/salicylate coating electrogenerated on a magnesium alloy
Loperena, Ana Paula; Saidman, Silvana Beatriz; Forero Lopez, Ana Deisy; Brugnoni, Lorena Inés; Lehr, Ivana Leticia
In this work, chitosan and chitosan/salicylate coatings were prepared by EPD on AZ91D Mg alloy to improve the corrosion resistance of the substrate in a simulated physiological solution. The effect of two concentrations of salicylate on the morphology and anticorrosive behaviour of the coatings was evaluated. The results showed that the addition of 0.50 g/L sodium salicylate to the chitosan formulation decreased the corrosion rate of the chitosan coating from 0.0025 ± 0.0003 to 0.0007 ± 0.0004 mA cm−2. From the results of electrochemical impedance spectroscopy (EIS) and Fourier Transform Infrared (FTIR) analysis, it can be concluded that the presence of salicylate in the coating leads to a self-healing effect that contributes to an improved corrosion protection. In addition, the presence of salicylate in the coating has been shown to increase antibacterial activity against both Gram-positive and Gram-negative bacteria. It has been demonstrated that salicylate can be released from the coating, which has an anti-inflammatory effect. In this way, a novel chitosan/salicylate coating was developed for the first time on a Mg alloy for bactericidal effect, release of salicylate and corrosion protection in simulated physiological solution. So far, chitosan and salicylate have not been investigated as a combination for the development of multifunctional coatings on Mg alloys. In addition, the self-healing effect of salicylate ions in a chitosan coating was established for the first time.
Unraveling the environmental influence on inter-annual fishery yield in a small-scale gillnet fishery under Rio de la Plata influence, South America
Unraveling the environmental influence on inter-annual fishery yield in a small-scale gillnet fishery under Rio de la Plata influence, South America
Jaureguizar, Andrés Javier; Cortés, Federico; Maiztegui, Tomás; Camiolo, Martina Daniela; Milessi Millan, Andres Conrado
The study focuses on understanding the influence of the environment on the relative yields of a gill net fishery operating in San Antonio Cape, Argentina. The research was conducted during five austral warm periods covering spring to summer from 2008 to 2013, aiming to assess the potential impacts of climate change on small-scale fisheries. The hierarchical species composition of 563 fishery landings encompasses 41 registered species. Among these species, Cynoscion guatucupa, Micropogonias furnieri, Mustelus schmitti, and Parona signata were found to be dominant. The species composition showed a similarity ranging from 36.22% to 57.59%, with significant differences observed among most of the warm seasons. The Multivariate Regression Tree analysis showed that the temperature was the variable with the greatest influence on the composition of the landings. During warmer conditions (>20.4 °C), the location of the draft became a significant factor, while variation in turbidity played a role in the northern zone. In colder warm conditions (<20.4 °C), the composition differed based on the volumes of Río de la Plata discharge. Specifically, the freshwater outflow was further differentiated by depth (∼9 m) at lower volumes (<20 649 m3/s) and by turbidity at intermediate values (20 649–22 908 m3/s). The vulnerability of the fishery was explained by a combination of specific ranges of various stressors. These stressors led to significant variations in the composition of fishery landings, ultimately affecting the economic income of the fishermen. By understanding the relationship between environmental factors and fishery yields, this study provides valuable insights into anticipating the potential impacts of climate change on small-scale, vulnerable fisheries.
Evaluation of fish biodiversity in the Beagle Channel (Tierra del Fuego-Argentina) using environmental DNA (eDNA) metabarcoding
Evaluation of fish biodiversity in the Beagle Channel (Tierra del Fuego-Argentina) using environmental DNA (eDNA) metabarcoding
Nardi, Cristina Fernanda; Llompart, Facundo Manuel; Chalde, Tomás; Bruno, Daniel Osvaldo; Ceballos, Santiago Guillermo
The fish biodiversity of the Beagle Channel is insufficiently assessed, poorly managed, and threatened by exotic species. Novel, rapid, and cost-effective DNA-based approaches are valuable for understanding fish communities and biomonitoring in remote areas. Here, we evaluate the potential of environmental DNA (eDNA) metabarcoding for assessing fish species composition by analyzing water samples from three sites along the Beagle Channel in the extreme south of Argentina. Fish biodiversity was estimated using high-throughput sequencing of 12S rRNA amplicons. First, a local reference database was generated, including 12S sequences of 48 spp. In total, 31 environmental sequences were recovered, belonging to 11 families, 15 genera, and 22 species. Six eDNA sequences did not match any species in our database. Multivariate analyses indicated that the relative species composition differed significantly among the sampled sites. However, the most abundant species detected were consistent with historical data on fish species recorded in the sites sampled. In conclusion, eDNA metabarcoding is sensitive, reliable, and suitable for studies on the presence and distribution of fish species in the area. We further strengthen the potential of eDNA metabarcoding as a biomonitoring tool.
Cuando el casamiento generaba oposición. Desigualdad, esclavitud y afrodescendencia en el contexto de la Real Pragmática de Matrimonios: Santa Fe, siglos XVIII-XIX
Cuando el casamiento generaba oposición. Desigualdad, esclavitud y afrodescendencia en el contexto de la Real Pragmática de Matrimonios: Santa Fe, siglos XVIII-XIX; When marriage generated opposition. Inequality, slavery and African descent in the context of the Real Pragmatics of Marriages: Santa Fe, 18th-19th centuries.
Silvestri, Noelia Camila
En los dominios coloniales de la Monarquía Hispánica, a medida que avanzaba el siglo XVIII, el mestizaje y la ilegitimidad representaban un desafío para las autoridades. En el contexto provisto por la aplicación en Indias de la “Pragmática Sanción para evitar el abuso de contraer matrimonios desiguales” indagaremos las oposiciones que enfrentaron parejas conformadas por al menos una persona afrodescendiente. A partir de disensos motivados por argumentos raciales, examinaremos las interpretaciones de la desigualdad en la sociedad santafesina entre fines del siglo XVIII e inicios del XIX. Desde fuentes judiciales y parroquiales, el análisis de casos posibilitará recuperar historias de vida de población esclavizada y de castas libres con el objetivo de analizar fenómenos como la movilidad ascendente y el blanqueamiento social.; In the colonial domains of the Hispanic Monarchy in the 18th century, miscegenation and illegitimacy represented an urgent matter for the authorities. In the context of the application in the Indies of the "Pragmatic Sanction to avoid the abuse of unequal marriages", we will investigate oppositions faced up by couples with at least one Afro-descendant person. Based on the dissent motivated by racial reasons, we will examine the interpretations of inequality in Santa Fe city between the late 18th century and the beginning of the 19th century. Based on judicial and parish sources, the analysis of cases recovers the life stories of enslaved and free caste populations intending to analyze upward mobility and social whitening.
Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
Augusto, Ingrid; Girard Dias, Wendell; Schoijet, Alejandra Cecilia; Alonso, Guillermo Daniel; Portugal, Rodrigo V.; de Souza, Wanderley; Jimenez, Veronica; Miranda, Kildare
Trypanosoma cruzi uses various mechanisms to cope with osmotic fluctuations during infection, including the remodeling of organelles such as the contractile vacuole complex (CVC). Little is known about the morphological changes of the CVC during pulsation cycles occurring upon osmotic stress. Here, we investigated the structure-function relationship between the CVC and the flagellar pocket domain where fluid discharge takes place-the adhesion plaque-during the CVC pulsation cycle. Using TcrPDEC2 and TcVps34 overexpressing mutants, known to have low and high efficiency for osmotic responses, we described a structural phenotype for the CVC that matches their corresponding physiological responses. Quantitative tomography provided data on the volume of the CVC and spongiome connections. Changes in the adhesion plaque during the pulsation cycle were also quantified and a dense filamentous network was observed. Together, the results suggest that the adhesion plaque mediates fluid discharge from the central vacuole, revealing new aspects of the osmoregulatory system in T. cruzi.
Arabidopsis pollen prolyl-hydroxylases P4H4/6 are relevant for correct hydroxylation and secretion of LRX11 in pollen tubes
Arabidopsis pollen prolyl-hydroxylases P4H4/6 are relevant for correct hydroxylation and secretion of LRX11 in pollen tubes
Sede, Ana Rocío; Wengier, Diego Leonardo; Borassi, Cecilia; Ricardi, Martiniano María; Somoza, Sofía Cristina; Aguiló, Rafael; Estevez, Jose Manuel; Muschietti, Jorge Prometeo
Major constituents of the plant cell walls are structural proteins that belong to the hydroxyproline-richglycoprotein (HRGP) family. Leucine-rich repeat extensin (LRX) proteins contain a leucine-richdomain and a C-terminal domain with repetitive Ser–Pro3–5 motifs that are potentially to be Oglycosylated.It has been demonstrated that pollen-specific LRX8–LRX11 from Arabidopsis thalianaare necessary to maintain the integrity of the pollen tube cell wall during polarized growth. InHRGPs, including classical extensins (EXTs), and probably in LRXs, proline residues are convertedto hydroxyproline by prolyl-4-hydroxylases (P4Hs), thus defining novel O-glycosylation sites. In thiscontext, we aimed to determine whether hydroxylation and subsequent O-glycosylation ofArabidopsis pollen LRXs are necessary for their proper function and cell wall localization in pollentubes. We hypothesized that pollen-expressed P4H4 and P4H6 catalyze the hydroxylation of theproline units present in Ser–Pro3–5 motifs of LRX8–LRX11. Here, we show that the p4h4-1 p4h6-1double mutant exhibits a reduction in pollen germination rates and a slight reduction in pollen tubelength. Pollen germination is also inhibited by P4H inhibitors, suggesting that prolyl hydroxylation isrequired for pollen tube development. Plants expressing pLRX11::LRX11-GFP in the p4h4-1 p4h6-1background show partial re-localization of LRX11–green fluorescent protein (GFP) from the pollentube tip apoplast to the cytoplasm. Finally, immunoprecipitation-tandem MSmass spectrometryanalysis revealed a decrease in oxidized prolines (hydroxyprolines) in LRX11–GFP in the p4h4-1p4h6-1 background compared with lrx11 plants expressing pLRX11::LRX11-GFP. Taken together,these results suggest that P4H4 and P4H6 are required for pollen germination and for properhydroxylation of LRX11 necessary for its localization in the cell wall of pollen tubes.
Performance of Hermetia illucens reared on pure, mixed, and sequentially mixed organic wastes regionally available in central Argentina
Performance of Hermetia illucens reared on pure, mixed, and sequentially mixed organic wastes regionally available in central Argentina
Picco, Romina Paula Andrea; Rinaudo, Karen; Pascual, Amalia Nelci Teresita; Schwab, Luisina; Luna, Agustin; Salvo, Silvia Adriana
Black soldier fly larvae (Hermetia illucens L.) are proficient decomposers of organic matter. We compared experiments with local substrates containing: domestic residues (DR), and brewery wastes (BW) in pure form, or mixed with in two different proportions (BW70 or BW30), and two sequential diets in which larvae were fed first DR and then BW (DR/BW) or vice versa (BW/DR). The effects of the diets on life cycle parameters for mass production of H. illucens larvae were evaluated using general linear mixed models, ANOVA and Kruskal-Wallis. The pure DR diet significantly prolonged the developmental period (47.8 days), although the pupal stage was shorter in larvae fed on this diet (13.8 days). Larval survival was similar between treatments (over 85%), but pupal survival tended to be higher in the mixed diets (over 96%). Larval weights differed significantly between treatments, with mixed diets promoting greater weight gains than pure and sequential diets. Larvae fed a DR/BW diet gained more weight than those fed a BW/DR diet. The highest mean larval weights (0.23 g) were observed in the mixed diet BW70, followed by the sequential diet DR/BW (0.22 g). Mixed diet BW70 also resulted in larger wings and bodies in both sexes. Sequential diet BW/DR and pure diet DR resulted in the smallest adult sizes. Sex ratios varied, favouring male emergence (1.8 M:0.2 F) in pure diet DR and in both sequential mixed diets (1.7 M:0.3 F and 1.3 M:0.7 F, respectively). This study highlights the importance of higher total protein plus carbohydrate concentrations in diets for optimal larval rearing, favouring mixed diets BW70. Carbohydrate-rich substrates should be provided earlier in the cycle to benefit larval and adult performance. Locally available waste vegetable or fruit peels, brewery waste, and spent yerba mate proved to be viable substrates for larval rearing, providing economic and environmental benefits for H. illucens rearing.
Analysis of Guard Cell Readouts Using Arabidopsis thaliana Isolated Epidermal Peels
Analysis of Guard Cell Readouts Using Arabidopsis thaliana Isolated Epidermal Peels
Pantaleno, Rosario; Schiel, Paula; García-Mata, Carlos; Scuffi, Denise
Stomata are pores surrounded by a pair of specialized cells, called guard cells, that play a central role in plant physiology through the regulation of gas exchange between plants and the environment. Guard cells have features like cell-autonomous responses and easily measurable readouts that have turned them into a model system to study signal transduction mechanisms in plants. Here, we provide a detailed protocol to analyze different physiological responses specifically in guard cells. We describe, in detail, the steps and conditions to isolate epidermal peels with tweezers and to analyze i) stomatal aperture in response to different stimuli, ii) cytosolic parameters such as hydrogen peroxide (H2O2), glutathione redox potential (EGSH), and MgATP-2 in vivo dynamics using fluorescent biosensors, and iii) gene expression in guard cell–enriched samples. The importance of this protocol lies in the fact that most living cells on epidermal peels are guard cells, enabling the preparation of guard cell–enriched samples.
Capítulo 10: Shincal de Quimivil - Londres, Catamarca
Capítulo 10: Shincal de Quimivil - Londres, Catamarca
Se trata de 12 micros de 3 minutos que tienen como hilo conductor la relación entre ciencia y turismo.
Capítulo 7: Ruta del artesano - Tafí del Valle
Capítulo 7: Ruta del artesano - Tafí del Valle
Se trata de 12 micros de 3 minutos que tienen como hilo conductor la relación entre ciencia y turismo.
Lo más lindo del paisaje es su gente
Lo más lindo del paisaje es su gente
Ciclo que retrata a trabajadores locales desde los más impactantes paisajes de Argentina.
Micromundos
Micromundos
Micromundos nos introduce en un universo a simple vista imperceptible, a través de las imágenes del microscopio de Barrido Zeiss Supra 55vp. Mientras vamos pasando de los grandes aumentos a la escala real, con la ayuda de distintas pistas, podremos comenzar a develar estos “micromundos”, encontrando en estas imágenes, que al principio parecían obras de ciencia ficción, elementos que forman parte de nuestra vida cotidiana.
El desierto que no fue - Trailer
El desierto que no fue - Trailer
En el año 1993 un grupo de arqueólogos encuentra una cueva única en la Puna argentina: el sitio CACAO se excava en función de encontrar evidencias de la ocupación humana entre 3000 y 3700 años antes del presente. 10 años más tarde, el arqueólogo Carlos Aschero da con una costilla de Milodón, un mamífero extinto de la era del hielo. La costilla tiene marcas que sugieren la intervención humana. Esta costilla arroja un fechado de 40.000 años. Esto sorprende a propios y extraños: el hallazgo data de 25.000 años de distancia entre lo que se sabe del poblamiento de América y lo que se puede comprobar. Esta historia narra las aventuras y desventuras de los arqueólogos frente a un hallazgo único que pone rigurosamente en discusión aquello que sabemos sobre cómo y cuándo se pobló el continente americano.
Cannabis Medicinal - Trailer
Cannabis Medicinal - Trailer
Todo tratamiento que contribuya a recuperar el estado de bienestar debe ser investigado, desarrollado y puesto al servicio de la población. La neurocientífica Silvia Kochen indaga sobre cómo el saber popular contribuye al conocimiento científico sobre el cannabis medicinal.
Páginas
