Bagasse-A major agro-industrial residue as potential resource for nanocellulose inks for 3D printing of wound dressing devices Chinga Carrasco, Gary; Ehman, Nanci Vanesa; Filgueira, Daniel; Johansson, Jenny; Vallejos, María Evangelina; Felissia, Fernando Esteban; Håkansson, Joakim; Area, Maria Cristina Sugarcane bagasse, an abundant residue, is usually burned as an energy source. However, provided that appropriate and sustainable pulping and fractionation processes are applied, bagasse can be utilized as a main source of cellulose nanofibrils (CNF). We explored in this study the production of CNF inks for 3D printing by direct-ink-writing technology. The CNF were tested against L929 fibroblasts cell line and we confirmed that the CNF from soda bagasse fibers were found not to have a cytotoxic potential. Additionally, we demonstrated that the alginate and Ca 2+ caused significant dimensional changes to the 3D printed constructs. The CNF-alginate grids exhibited a lateral expansion after printing and then shrank due to the cross-linking with the Ca 2+ . The release of Ca 2+ from the CNF and CNF-alginate constructs was quantified thus providing more insight about the CNF as carrier for Ca 2+ . This, combined with 3D printing, offers potential for personalized wound dressing devices, i.e. tailor-made constructs that can be adapted to a specific shape, depending on the characteristics of the wound healing treatment.

Solidaridad y huelga obrera. la sentencia sobre Sacco y Vanzetti y su repercusión en el movimiento obrero de Bahía Blanca (1927); Solidarity and workers strike. The sentence on Sacco and Vanzetti and its repercussion in the workers movement of Bahía Blanca (1927) Becher, Pablo Ariel; Martín, Lucio Emmanuel Dentro de los recursos semióticos disponibles en los sistemas de mensajería instantánea, los emojis son especialmente apreciados por los usuarios gracias al variado repertorio que ofrecen y a su capacidad para dinamizar la interacción. Ante el crecimiento de su uso, nos planteamos la necesidad de disponer de una técnica metodológica que permita no solo identificar los emojis más frecuentes (es decir, los que tengan un uso más extendido en una comunidad de habla), sino también sus principales funciones e interpretaciones más usuales. Por ello, en este trabajo presentamos, por un lado, una metodología que hace posible recuperar los emojis utilizados con mayor frecuencia por parte de un grupo de usuarios de WhatsApp y, por otro, un modelo de exploración e interpretación de estos primeros datos obtenidos. Nuestro diseño metodológico se aplica a la recogida de una muestra comparativa de dos variedades del español: español de España y español de Argentina.; Within the semiotic resources available in instant messaging systems, emojis are especially appreciated by users due to the varied repertoire that offer as for its ability to dynamize the interaction. With the growth of its use, we consider the need for a methodology technique that allows not only to identify the most frequent emojis (i.e., those who have a more extended use in a speech community), but also its main functions and common interpretations. Therefore, in this work, we present, on the one hand, a methodology that makes it possible to recover the emojis used most frequently by a group of WhatsApp users and, on the other, an exploration and interpretation of these first data obtained. We applied our methodological design for the collection of a comparative sample of two Spanish varieties: Spanish of Spain and Spanish of Argentina.

In vitro effects of progesterone and the synthetic progestin medroxyprogesterone acetate on vascular remodeling Cutini, Pablo Hernan; Massheimer, Virginia Laura In this work we tested the hypothesis whether progesterone (Pg)or the synthetic progestin medroxyprogesterone acetate (MPA) could beinvolved in the regulation of events involved in vascular remodeling.Results revealed an enhancement in the capillary-like tubes formationinduced by both progestogens. Unlike MPA, Pg acts through VEGF, nitricoxide, PI3K and ERK1/2 signaling pathways. However, the MPA effectdepends on platelet activation. Under stress conditions, theproangiogenic action of Pg and MPA was sustained. The progestogensexhibit the ability to prevent vascular smooth muscle cells (VSMC)osteogenic transdifferentiation. Besides this antiosteogenic action, onbone cells the progestogens induced osteoblast maturation andmineralization. The mechanism of action of both steroids on vascular andbone cells involves the participation of progesterone receptor. The datapresented in this work provide evidence that the progestogens reduceosteogenic-like transdifferentiation of VSMC and promote angiogenesiswith a slight different mechanism of action elicited by each steroid.

Method for phenol recovery from “alperujo”: numerical optimization and predictive model Soberon, Laura Fabiana; Carelli Albarracin, Amalia Antonia; Gonzalez, Maria Teresa; Ceci, Liliana Noemí “Alperujos” of Arbequina olives harvested in 2010 and 2011 and of Nevadillo olives in 2012 were evaluated in order to reduce their pollutant load by recovering the phenols with antioxidant properties. First, three successive extractions with water (4 °C, 120 min) were carried out to evaluate by spectrophotometry the total phenol contents, which significantly varied with cultivar and harvest year. The combined aqueous extracts of “alperujo” (combined-AEA) showed high pollutant loads with volatile solids to total solids ratios close to 1 suggesting high organic material contents and very low total phenols to chemical oxygen demand ratios. Second, phenols were recovered from the combined-AEA using ethyl acetate. By experimental design, the following conditions were found to maximize phenol recovery: temperature = 20 °C, time = 120 min, pH of aqueous extract = 2.00 and solvent/extract ratio (v/v) = 3:1. Up to 41.6% of the phenols were recovered from the combined-AEA after three successive extractions using recycled solvent.

Green Peas in X-Rays Svoboda, Jiri; Douna, Vanesa Mariel; Orlitová, Ivana; Ehle, Matthias Green Peas represent a population of compact, highly star-forming dwarf galaxies at redshifts z ∼ 0.2-0.3 that have recently been found to show signatures of ultraviolet ionizing radiation leakage. They are being considered as analogs to high-redshift star-forming galaxies, possibly responsible for cosmic reionization. Despite intensive studies of Green Peas in the ultraviolet and optical domains, their X-ray properties have only so far been probed by nearby analogs. In this paper, we present the first measurements of Green Peas in the X-ray domain to constrain their spectral properties and fluxes at high energies. We analyzed XMM-Newton observations of three Green Pea sources. For two of them, we found an X-ray luminosity exceeding by a half-order of magnitude its predicted value, derived from the star formation rate and metallicity. Only an upper limit of the X-ray luminosity was derived for the third studied galaxy. Our results indicate that at least some Green Peas produce copious amounts of highly energetic photons, larger than detected in other star-forming galaxies. We discuss possible physical scenarios for the measured X-ray excess, including the presence of a hidden active galactic nucleus, a larger population of X-ray binaries, or ultra-luminous X-ray sources. Future spatially resolved X-ray images will discriminate between the models. Larger Green Pea samples will provide a possible link between the X-ray properties and the leaking ultraviolet radiation.

Holographic interference in atomic photoionization from a semiclassical standpoint López, Sebastián David; Arbo, Diego A theoretical study of the interference pattern imprinted on the doubly differential momentum distribution of the photoelectron due to atomic ionization induced by a short laser pulse is developed from a semiclassical standpoint. We use the semiclassical two-step model of Shvetsov-Shilovski et al. [Phys. Rev. A 94, 013415 (2016)2469-992610.1103/PhysRevA.94.013415] to elucidate the nature of the holographic structure. Three different types of trajectories are characterized during the ionization process by a single-cycle pulse with three different types of interferences. We show that the holographic interference arises from the ionization yield only during the first half cycle of the pulse, whereas the coherent superposition of electron trajectories during the first half cycle and the second half cycle gives rise to two other kinds of intracycle interference. Although the picture of interference of a reference beam and a signal beam is adequate, we show that our results for the formation of the holographic pattern agree with the glory rescattering theory of Xia et al. [Phys. Rev. Lett. 121, 143201 (2018)10.1103/PhysRevLett.121.143201]. We probe the two-step semiclassical model by comparing it to the numerical results of the time-dependent Schrödinger equation.

Prosopis alba seed flour improves vascular function in a rabbit model of high fat diet-induced metabolic syndrome Cattaneo, Florencia; Roco, Julieta; Alarcón, Gabriela del Jesús; Isla, Maria Ines; Jerez, Susana Josefina Aims: Prosopis alba flour is a natural source of nutrient and phytochemicals with potential effects on cardiovascular risk factors. The aim of this work was to examine the effects of dietary supplementation with Prosopis alba seed flour (Pr-Feed) on a high fat diet (FD)-induced rabbit model of metabolic syndrome. Main methods: Rabbits were separated in four groups: fed regular diet (CD); CD supplemented with Pr-Feed; fed on 18 % FD; FD supplemented with Pr-Feed. All diets were administrated for 6 weeks. After the feeding period body weights, mean blood pressure, heart rate and visceral abdominal fat (VAF) were determined; glucose tolerance test (GTT) was performed; total cholesterol (TC), HDL-cholesterol, LDL-cholesterol, triglycerides (TG), fasting glucose (FG), aspartate amino transferase, alanine amino transferase, bilirubin and creatinine were measured in serum. Abdominal aorta was excised and vascular function was assessed by acetylcholine relaxation and contractile response to KCl, norepinephrine and angiotensin II. Key findings: Phytochemical analyses showed that the main compounds of Pr-Feed were apigenin C-glycosides. FD increased VAF, FG, TG, reduced HDL-cholesterol and induced abnormal GTT. Pr-Feed addition to FD did not modify these alterations. Aortic rings from rabbits fed on FD exhibited an impaired relaxation-response to acetylcholine and increased agonist vasoconstrictor responses. Pr Feed-supplemented FD improved the response to acetylcholine, and prevented the increase of the contractile response to KCl, norepinephrine and angiotensin II. Significance: Results suggest that dietary supplementation with Pr-Feed, rich in apigenin C-glycosides, has vascular protector properties and could be used to prevent vascular alterations characterizing the metabolic syndrome.

Microbial mat contribution to the formation of an evaporitic environment in a temperate-latitude ecosystem Perillo, Vanesa Liliana; Maisano, Lucia; Martinez, Ana María; Quijada, Isabel Emma; Cuadrado, Diana Graciela An evaporitic environment is characterized by having high salinity, climatic, and hydrological factors that promote a negative water balance; however, biological factors may also influence their development. Modern coastal flat Paso Seco (40°33′S; 62°14′W) is located in a semi-arid region with low precipitation and dry winds coming mainly from the NW. The site is an old tidal channel, which nowadays behaves like a shallow coastal saline-like basin, separated from the sea by a sand barrier, which the sea periodically overcomes, flooding the flat with eventual water evaporation. Microbial mats of up to 1 cm thick colonize the sandy sediments of this evaporitic environment. Water samples were taken during five field trips (2017–2018) from interstitial water of the flat, a tidal creek that crosses the flat, and two shallow tidal depressions (TDs) within the flat with different degrees of evaporation. In comparison to the sea, the maximum salinity values measured in Austral spring (September 2017) in the tidal creek were doubled, tripled in interstitial water, and 5.9 to 8 times higher in TDs. Ionic concentration denotes that evaporite chemical divides are followed as water evaporates, corresponding to the presence of CaCO3, gypsum and halite found in TDs. On-site permeability of microbial mat-covered surfaces presented semi-pervious properties. Microbial mat presence is condition for CaCO3, gypsum, and halite precipitation as they allow for water retention and its consequent evaporation due to the impermeability they confer to the sedimentary surface. Thus, microbial mats are a biological factor affecting the development of an evaporitic environment.

A new species and the record of Hemihegetotherium (Notoungulata, Hegetotheriidae) in the Middle to Late Miocene of Patagonia, Argentina Vera, Bárbara Soledad The fossils of Hegetotheriinae notoungulates studied herein were recovered from the Collón Curá Formation at Cerro Zeballos (Chubut)and Comallo (Río Negro), which allow to report the first record of Hemihegetotherium from the Middle to Late Miocene of Patagonia (Argentina)and to describe a new species into this genus. The new taxon Hemihegetotherium tantillum sp. nov. is characterized by having a small size, an overlapped C labial to the P1, a squared and less imbricate P3–4, a not molarized P4, p1 positioned labial to p2, a lingually concave and labially bilobed p2, and more acute and pronounced mesial and distal ends on lower molars. The new species is morphologically similar to H. trilobus Croft and Anaya from the Middle Miocene fauna of Quebrada Honda (Bolivia), both sharing three synapomorphies. On the other hand, the holotype of Propachyrukhos robustus Roth is recognized as Hemihegetotherium cf. tantillum sp. nov., which implies that the genus, and tentatively the new species, are also present in the type locality of the Collón Curá Formation at Neuquén Province from where the holotype of P. robustus came from. This new report is relevant not only because it broadens the knowledge of Hegetotheriinae from the Collón Curá Formation and Middle to Late Miocene of Patagonia, but also because it involves the southernmost record of Hemihegetotherium in South America and a close affinity between the faunas of Patagonia and Quebrada Honda.

Optimización de la eficiencia energética aplicando confort térmico adaptativo en un edificio de oficinas público en San Juan-Argentina; Energy efficiency optimization through adaptive thermal comfort in a public office building in San Juan, Argentina Arballo, Bruno Damián; Kuchen, Ernesto; Chuk, Oscar Daniel Las edificaciones son responsables del 40% de la demanda mundial de energía y de emisiones de CO2. En Argentina, los edificios representan más del 40% del consumo total anual de energía. Las estrategias de ahorro de energía pueden provocar disconformidad térmica en los habitantes de los edificios. El objetivo de este trabajo es optimizar las temperaturas de “set point” diarias basadas en los perfiles de ocupación y la evolución del clima exterior para encontrar el equilibrio entre la eficiencia energética y el confort térmico de un edificio de oficinas público en San Juan, Argentina. El modelo tiene en cuenta la capacidad de adaptación térmica de los habitantes considerando la variación de la temperatura exterior. El método propuesto combina el análisis de monitoreo in situ, que introduce datos subjetivos de ocupación, con la herramienta de simulación energética para edificios Energy Plus. Los resultados de la optimización muestran alrededor de un 26% de ahorro de energía, manteniendo condiciones aceptables de confort térmico.; Buildings are responsible for 40% of global energy demand and CO2 emissions. In Argentina, buildings represent more than 40% of total annual energy consumption. Energy-saving strategies can cause thermal discomfort for building inhabitants. The aim of this research is to optimize daily set point temperatures based on occupancy profiles and changes in outdoor weather to find a balance between energy efficiency and thermal comfort in a public office building in San Juan, Argentina. The model takes into account the thermal adaptation capacity of the inhabitants considering outdoor temperature variation. The proposed method combines spot monitoring analysis, which introduces subjective occupancy data, with the Energy Plus building energy simulation tool. Optimization results show around 26% energy savings, while maintaining acceptable thermal comfort conditions.

Analysis of biological traits of Anagyrus cachamai and Anagyrus lapachosus to assess their potential as biological control candidate agents against Harrisia cactus mealybug pest in Puerto Rico Aguirre, María Belén; Logarzo, Guillermo Alejandro; Triapitsyn, Serguei V.; Diaz Soltero, Hilda; Hight, Stephen D.; Bruzzone, Octavio Augusto The Harrisia cactus mealybug (HCM), Hypogeococcus sp. (Hemiptera: Pseudococcidae), is devastating native cacti in Puerto Rico and threatens cacti throughout the Caribbean, Mexico, Central and North America. In South America, its native area, various natural enemies keep HCM under control. Two South American parasitoids, Anagyrus cachamai Triapitsyn, Logarzo & Aguirre and A. lapachosus Triapitsyn, Aguirre & Logarzo (Hymenoptera: Encyrtidae), were selected as potential biological control agents. Rearing protocol to conduct mass production, specificity studies and several aspects of the biology of these species were studied under laboratory conditions in Argentina. Anagyrus cachamai and A. lapachosus successfully attacked early instars of Hypogeococcus sp., have a balanced sex ratio, exhibited a development time synchronized with that of the host, and presented differences in their reproductive biology and development time. All these characteristics make these parasitoids promising candidates for introduction as biological control agents against the HCM pest in Puerto Rico.

Por amor al arte: El problema de la autonomía en el asociacionismo cultural bonaerense(La Asociación Cultural de Bahía Blanca, 1919-1927) Agesta, María de Las Nieves El presente trabajo se propone abordar el problema de las relaciones entre las asociaciones culturales del interior de la provincia de Buenos Aires, el mercado y el Estado a principios del siglo XX. El doble reclamo de autonomía ligado al asociacionismo moderno y a la configuración de una esfera específica de las artes, suponía para ellas una permanente fuente de tensiones que las enfrentaba al dilema de sostener su independencia y sufrir la endémica carencia de fondos o acudir a instancias externas de provisión de recursos y así garantizar su funcionamiento. Esta situación fue la que enfrentó la Asociación Cultural de Bahía Blanca entre 1919 y 1927. Surgida en una ciudad intermedia de sudoeste bonaerense, la agrupación debió sobrevivir en diálogo con un Estado municipal donde las cuestiones culturales no eran objeto de una política sistemática y en un medio en el que el circuito de la música académica continuaba aún vinculado fuertemente al diletantismo. Sumado a ello, los incipientes mecanismos del mercado cultural pugnaban por introducir una lógica económica que se oponía a la primacía del desinterés que se estaba consolidando como principio de legitimidad en el mundo de las artes.; This article examines the problem of the relations between cultural associations, the market and the State in the province of Buenos Aires at the beginning of the 20th century. The double claim of autonomy linked to modern associationism and to the configuration of a specific sphere of the arts, was a permanent source of tensions that confronted them with the dilemma of sustaining their independence and suffering the endemic lack of funds or asking for external resources and thus assure its survival. This situation was faced by the Asociación Cultural de Bahía Blanca between 1919 and 1927. Emerged in an intermediate city of southwest Buenos Aires, this entity had to survive in dialogue with a municipal state where cultural issues were not subject of a systematic policy and in an environment in which the circuit of academic music continued to be strongly linked to dilettantism. Added to this, the incipient mechanisms of the cultural market struggled to introduce an economic logic that opposed the primacy of "disinterest" that was consolidating as a principle of legitimacy in the world of arts.

Outcome of Applying the ESC 0/1-hour Algorithm in Patients With Suspected Myocardial Infarction Twerenbold, Raphael; Costabel, Juan Pablo; Nestelberger, Thomas; Campos, Roberto; Wussler, Desiree; Arbucci, Rosina; Cortes, Marcia; Boeddinghaus, Jasper; Baumgartner, Benjamin; Nickel, Christian H.; Bingisser, Roland; Badertscher, Patrick; Puelacher, Christian; du Fay de Lavallaz, Jeanne; Wildi, Karin; Rubini Giménez, Maria; Walter, Joan; Meier, Mario; Hafner, Benjamin; Lopez Ayala, Pedro; Lohrmann, Jens; Troester, Valentina; Koechlin, Luca; Zimmermann, Tobias; Gualandro, Danielle M.; Reichlin, Tobias; Lambardi, Florencia; Resi, Silvana; Alves de Lima, Alberto Enrique; Trivi, Marcelo Ricardo; Mueller, Christian Background: The European Society of Cardiology (ESC) recommends the 0/1-h algorithm for rapid triage of patients with suspected non–ST-segment elevation myocardial infarction (MI). However, its impact on patient management and safety when routinely applied is unknown. Objectives: This study sought to determine these important real-world outcome data. Methods: In a prospective international study enrolling patients presenting with acute chest discomfort to the emergency department (ED), the authors assessed the real-world performance of the ESC 0/1-h algorithm using high-sensitivity cardiac troponin T embedded in routine clinical care and its associated 30-day rates of major adverse cardiac events (MACE) (the composite of cardiovascular death and MI). Results: Among 2,296 patients, non–ST-segment elevation MI prevalence was 9.8%. In median, 1-h blood samples were collected 65 min after the 0-h blood draw. Overall, 94% of patients were managed without protocol violations, and 98% of patients triaged toward rule-out did not require additional cardiac investigations including high-sensitivity cardiac troponin T measurements at later time points or coronary computed tomography angiography in the ED. Median ED stay was 2 h and 30 min. The ESC 0/1-h algorithm triaged 62% of patients toward rule-out, and 71% of all patients underwent outpatient management. Proportion of patients with 30-day MACE were 0.2% (95% confidence interval: 03% to 0.5%) in the rule-out group and 0.1% (95% confidence interval: 0% to 0.2%) in outpatients. Very low MACE rates were confirmed in multiple subgroups, including early presenters. Conclusions: These real-world data document the excellent applicability, short time to ED discharge, and low rate of 30-day MACE associated with the routine clinical use of the ESC 0/1-h algorithm for the management of patients presenting with acute chest discomfort to the ED.

Diet-Induced Pulmonary Inflammation and Incipient Fibrosis in Mice: a Possible Role of Neutrophilic Inflammation Della Vedova, Maria Cecilia; Soler Garcia, Florencia Martina; Muñoz, Marcos David; Fornes, Miguel Walter; Gomez-Mejiba, Sandra Esther; Gomez, Nidia Noemí; Ramirez, Dario Chicken fat and fructose are added into food-processing to reduce costs and enhance acceptability; however, these additives turn food into unhealthy and hypercaloric meals. Herein we have hypothesized that chronic feeding with chicken fat and fructose, together or by separate, can cause pulmonary redox and inflammatory changes. These changes are particularly related to neutrophils and myeloperoxidase, with consequent changes in the organ histophysiology. To test this hypothesis, we fed mice for 16 weeks with either control food (low-fat diet, LFD) or control food supplemented with 22% chicken fat and with or without 10% fructose in the drinking water. At the end of the feeding regimen, we measured redox and inflammatory changes in the lung with particular emphasis on neutrophil accumulation/activation and molecular-histological markers of fibrosis. Our results suggest that a diet supplemented with chicken fat and fructose causes additive effects on pulmonary oxidative stress, inflammation, and a pro-fibrotic status. Neutrophilic inflammation may play a critical role in pulmonary pathology associated with metabolic syndrome.

New species of Capronia (Herpotrichiellaceae, Ascomycota) from Patagonian forests, Argentina Sanchez, Romina Magali; Miller Andrew, N.; Bianchinotti, Maria Virginia Three new species belonging to Capronia are described from plants native to the Andean Patagonian forests, Argentina. The first record of C. chlorospora in South America is also reported. The identity of the three new species is based on detailed morpho-anatomical observations as well as analyses of ITS and LSU nuclear rDNA. A key to the Capronia species present in Argentina is provided.

A combinatorial analysis of the permutation and non-permutation flow shop scheduling problems Rossit, Daniel Alejandro; Vásquez, Óscar C.; Tohmé, Fernando Abel; Frutos, Mariano; Safe, Martin Dario In this paper we introduce a novel approach to the combinatorial analysis of flow shop scheduling problems for the case of two jobs, assuming that processing times are unknown. The goal is to determine the dominance properties between permutation flow shop (PFS) and non-permutation flow shop (NPFS) schedules. In order to address this issue we develop a graph-theoretical approach to describe the sets of operations that define the makespan of feasible PFS and NPFS schedules (critical paths). The cardinality of these sets is related to the number of switching machines at which the sequence of the previous operations of the two jobs becomes reversed. This, in turn, allows us to uncover structural and dominance properties between the PFS and NPFS versions of the scheduling problem. We also study the case in which the ratio between the shortest and longest processing times, denoted ρ, is the only information known about those processing times. A combinatorial argument based on ρ leads to the identification of the NPFS schedules that are dominated by PFS ones, restricting the space of feasible solutions to the NPFS problem. We also extend our analysis to the comparison of NPFS schedules (with different number of switching machines). Again, based on the value of ρ, we are able to identify NPFS schedules dominated by other NPFS schedules.

Detection and first molecular characterization of bovine papular stomatitis virus in dairy calves in Argentina Micheloud, Juan Francisco; Aguirre Castro, Laura Sabrina; Sandoval, Gabriela Virginia; Avellaneda Caceres, Agustin; Diodati, Julian Angel; Peralta, Andrea Verónica Bovine papular stomatitis virus (BPSV) is a parapoxvirus associated with papular and erosive lesions on the muzzle, lips, and oral mucosa of cattle. BPSV infection occurs worldwide; however, it has still not been unequivocally diagnosed. The present report describes an outbreak of BPSV infection affecting dairy calves in northwestern Argentina and provides the first molecular characterization of this virus in the country. The disease was detected in a dairy farm, affecting 33 calves between 2 and 20 days of age. The signs included reddish papules, ulcers, and scabby proliferative lesions on muzzle, lips, and oral mucosa. The affected calves resisted to being fed due to severe local pain. Two necropsies were performed; papulas and ulcers were observed in ruminal and omasal mucosa. Histologically, the affected areas of the skin showed acanthosis, spongiosis, and parakeratotic hyperkeratosis with adjacent focally extensive ulcers and multifocal inflammatory infiltrate in the epidermis. Eosinophilic inclusion bodies were detected in the cytoplasm of epithelial cells. DNA extracted from scab samples was analyzed by PCR using pan-parapoxvirus primers for the B2L gene. The sequence analysis revealed 99%, 85%, and 84% similarity with BPSV, Pseudocowpox virus, and Orf virus, respectively. A phylogenetic tree constructed using the B2L sequence showed that the virus clustered with BPSV isolates. Although clinical cases compatible with BSPV infection have been frequently described in Argentina, the present report is the first to identify the agent associated with cattle disease in the country.

Controversias en torno a la terminalidad educativa y la empleabilidad de mujeres destinatarias de programas sociales: Virajes de gestión en torno al programa Hacemos Futuro Voria, María Andrea; Míguez, María Eugenia En este artículo nos proponemos indagar las controversias en torno a la terminalidad educativa y la empleabilidad en programas sociales del Ministerio de Desarrollo Social de la Nación (MDS), en particular el ex Programa Ingreso Social con Trabajo –Argentina Trabaja– (PRIST-AT) (2009-2018) y su línea programática Ellas Hacen (EH) (2013-2018), unificados desde febrero del 2018 bajo el programa Hacemos Futuro (HF). El objetivo del artículo consiste en problematizar, desde una perspectiva de género, la terminalidad educativa y la empleabilidad en las mujeres destinatarias, especialmente en el “período bisagra” entre el fin de la gestión kirchnerista y los primeros años de gobierno de la Alianza Cambiemos. El corpus de análisis se basó en la revisión de fuentes secundarias (normativa, informes de gestión y pedidos de informes); fuentes primarias a partir de entrevistas en profundidad a cuadros intermedios del MDS y el desarrollo de dos grupos focales con mujeres titulares del ex AT como de EH, en los distritos de Tigre y San Fernando de la Provincia de Buenos Aires, durante 2017 y 2018. Los resultados que hemos, provisoriamente, alcanzado dejan planteadas una serie de controversias relativas al peso diferencial que adquieren entre uno y otro programa (AT-EH), como entre una y otra gestión del MDS, los componentes de terminalidad educativa y empleabilidad sobre las titulares, y sus implicancias en términos de reconocimiento social, responsabilidades de cuidado e integridad frente a la problemática de la violencia de género.; In this article we propose to investigate the controversies about educational terminality and employability in social programs of the Ministry of Social Development of the Nation (MSD), particularly, the ex “Programa Ingreso Social con Trabajo - Argentina Trabaja” (PRISTAT) (2009-2018) and its operational guideline “Ellas Hacen” (2013- 2018), unified under the program Hacemos Futuro (HF) since February 2018. The objective of this article consists of putting into question,from a gender perspective, the educational terminality and employability in women receiving, specially, during the hinge period between the ending of the Kirchnerist government and the first years of the Alianza Cambiemos government. The corpus of the exploration is based on the revision of secondary sources (rules, management reports and reporting orders); primary sources (in-deph interviews to middle management personnel of the MDS, and two focus groups of women receiving of the mentioned social programs ex-AT and EH, in Tigre and San Fernando neigborhoods, Province of Buenos Aires), during 2017 and 2018. The provisionally results we obtained raise an issue about a chain of controversies related to the different weight that acquire, between one and the other program (AT-EH) as well between one and the other management ministery, the components of educational terminality and employability on the women receiving and its specific consequences in social recognition, care responsabilities, and dignity in cases of gender violence.

Periodic dietary restriction ameliorates amyloid pathology and cognitive impairment in PDAPP-J20 mice: Potential implication of glial autophagy Gregosa, Amal; Vinuesa, María Angeles; Todero, María Florencia; Pomilio, Carlos Javier; Rossi, Soledad Paola; Bentivegna, Melisa Inés María; Presa, Jessica; Wenker, Shirley Denise; Saravia, Flavia Eugenia; Beauquis, Juan Dietary restriction promotes cell regeneration and stress resistance in multiple models of human diseases. One of the conditions that could potentially benefit from this strategy is Alzheimer´s disease, a chronic, progressive and prevalent neurodegenerative disease. Although there are no effective pharmacological treatments for this pathology, lifestyle interventions could play therapeutic roles. Our objectives were 1) to evaluate the effects of dietary restriction on cognition, hippocampal amyloid deposition, adult neurogenesis and glial reactivity and autophagy in a mouse model of familial Alzheimer´s disease, and 2) to analyze the role of glial cells mediating the effects of nutrient restriction in an in vitro model. Therefore, we established a periodic dietary restriction protocol in adult female PDAPP-J20 transgenic mice for 6 weeks. We found that dietary restriction, not involving overall caloric restriction, attenuated cognitive deficits, amyloid pathology and microglial reactivity in transgenic mice when compared with ad libitum-fed transgenic animals. Also, transgenic mice showed an increase in the astroglial positive signal for LC3, an autophagy-associated protein. In parallel, hippocampal adult neurogenesis was decreased in transgenic mice whereas dietary-restricted transgenic mice showed a neurogenic status similar to controls. In vitro experiments showed that nutrient restriction decreased astroglial and, indirectly, microglial NFκB activation in response to amyloid β peptides. Furthermore, nutrient restriction was able to preserve astroglial autophagic flux and to decrease intracellular amyloid after exposure to amyloid β peptides. Our results suggest neuroprotective effects of nutrient restriction in Alzheimer´s disease, with modulation of glial activation and autophagy being potentially involved pathways.

Novel Synthesis Without Separation and Purification Processes of Carbon Dots and Silver/Carbon Hybrid Nanoparticles Arroyave Rodriguez, Jeison Manuel; Springer, Valeria Haydee; Centurión, María Eugenia This paper presents two bottom-up methods for the green synthesis of spherical carbon dots (Cdots) and silver/Cdots (Ag@Cdots) nanoparticles at alkaline medium. Both syntheses are carried out without separation and purification steps with a microwave exposure time between 30 and 60 s. In both case a natural product as glucose was used as precursor and capping agent. The obtained Cdots and Ag@Cdots hybrid are spherical in shape and monodisperse, with average particle size of lower than 10 nm and 13 nm respectively. In comparison with the methods previously reported, which need high energy consumption and/or long reaction time. The proposed methodologies are simple, low cost, environment-friendly and in accordance with green chemistry research. The morphology, composition and structure of the obtained nanoparticles were characterized by Zeta Potential and hydrodynamic size measurements, transmission electron microscopy (TEM), X-ray diffraction (XRD), ATR-FTIR spectrophotometry and thermogavimetric analysis (TGA). The synthesized nanoparticles were applied to sense tetracycline residues taking into account their fluorescent properties.