Vocabularios polémicos y lenguajes polisémicos en la democracia argentina: a propósito de la aparición de Palabras Políticas de Reano y Smola y del Diccionario del Léxico Corriente de la Política Argentina de Adelstein y Vommaro Lesgart, Cecilia Dos libros, escritos ambos en coautoría y de reciente aparición, están recorridos por una línea argumental poderosa. Ambos tratan sobre el vocabulario que se ha ido produciendo para hablar de la política en una Argentina que se piensa centralmente democrática durante estos últimos 30 años. Y de las maneras en las que se ha ido alterando y renovando ese léxico político, para dar cuenta de transformaciones políticas significativas, para hablar de la emergencia de actores, de las prácticas sociales, políticas y económicas, para estimular modificaciones en lenguajes que no alcanzan para caracterizar el mundo público circundante, o para provocar, a través de palabras, innovaciones políticas.

Las redes de políticas como herramientas de análisis: notas a partir de intervenciones recientes en materia de desarrollo rural en Argentina (2008-2014); Policy networks as tools of analysis. Notes from recent interventions on rural development in Argentina (2008-2014) Nogueira, Maria Elena Las redes de políticas (policy networks) llevan ya algunos años en la agenda de conceptos de la Ciencia Polí- tica. La posibilidad de observar de un modo crítico las relaciones inter-gubernamentales en diferentes niveles, identificando actores y acciones en contextos diversos resulta una herramienta de gran interés analítico. En este trabajo, se propone un recorrido por parte de la literatura sobre las redes de políticas para dar cuenta de aquellas existentes en materia de políticas de desarrollo rural en Argentina en el último tiempo. El artículo se compone de una Introducción; un segundo apartado en el que se presentan los aspectos teóricos vinculados con el Estado, las políticas públicas y las redes; un tercer apartado señalando algunas características de las intervenciones en materia de desarrollo rural en el país y un cuarto, dedicado al análisis de algunas experiencias en red. Finalmente, se presentan conclusiones que enfatizan el rol protagónico del Estado en las redes y, asimismo, las dificultades y desafíos que presentan estos espacios intermedios en la relación sistema político/ sociedad en cuanto aparecen, la mayoría de las veces, como redes «en el papel».; Policy networks already have been part of the in Political Science for many years. The ability to look critically inter-governmental relationships at different levels in order to identify actors and actions in several contexts is a tool of great analytical value. In this paper, a route is proposed by the literature on policy networks to account for those existing in the field of rural development policies in Argentina in recent times. This paper presents an introduction, after that, a second section in which theoretical aspects connected with the State, public policies and networks are developed; a third paragraph indicates some characteristics of interventions in rural development in the country and, a fourth section is dedicated to analyze a number of experiences. Finally, some conclusions emphasize the leading role of the State in policy network and also the difficulties and challenges that these have as in-between the relationship political system /society in which they are present, most of the time, as networks «on paper».

Positional scanning synthesis of a Peptoid library yields new inducers of apoptosis that target Karyopherins and Tubulin Vendrell Navarro, Gloria; Rúa, Federico; Bujons, Jordi; Brockmeyer, Andreas; Janning, Petra ; Ziegler, Slava; Messeguer, Angel; Waldmann, Herbert We describe the synthesis of a library of 11 638 N-alkylglycine peptoid trimers in a positional scanning format with adjustment of reaction conditions to account for different reactivities of the monomer building blocks. Evaluation of the library by high-content phenotypic screening for modulators of the cytoskeleton and mitosis resulted in the identification of two apoptosis-inducing peptoids, which, despite their structural similarity, target different proteins and cellular mechanisms. Whereas one peptoid binds to karyopherins, which mediate nuclear transport, the other N-alkylglycine trimer binds tubulin at the vinca alkaloid binding site.

Optimized extraction of a single-chain variable fragment of antibody by using aqueous micellar two-phase systems Pellegrini Malpiedi, Luciana; Nerli, Bibiana Beatriz; Taqueda, Maria, E. S.; Abdalla, Dulcineia S. P.; Pessoa Jr., Adalberto In this work, the purification of a single-chain variable fragment (scFv) of an antibody by using liquid–liquid extraction in aqueous micellar two-phase systems was optimized by means of central composite design. Protein partitioning assays were performed by using the selected system composition in previous works: Triton X-114 at 4% wt/wt, yeast fermentation supernatant at 60% wt/wt, McIlvaine buffer pH 7.00. The other system component concentrations, Cibacron Blue F3GA (CB), Fabsorbent™ F1P HF (HF) and NaCl, were selected as independent variables. ScFv recovery percentage (%R) and purification factor (PF) were selected as the responses. According to the optimization process both, scFv recovery percentage and purification factor were favored with the addition of HF and NaCl in a range of concentrations around the central point of the second central composite design (HF 0.0120% w/w, CB 0.0200% w/w, NaCl 0.200% w/w). These experimental conditions allowed the concentration and pre-purification of scFv in the micelle-rich bottom phase of the systems with a recovery percentage superior to 88% and a purification factor of approximately 3.5. These results improved the previously presented works and demonstrated the convenience of using aqueous micellar two-phase systems as a first step in the purification of scFv molecules.

La vida después de la muerte: crisis de reproducción y estructura social de un grupo evangélico; Life after death: crisis of reproduction and social structure of an evangelical group Espinosa, Mariana Esther Este artículo intenta comprender el sentido de los conflictos y cambios que derivaron de la muerte de dos líderes de un grupo evangélico conocido como Hermanos Libres en Santiago del Estero (Argentina). Sostendré que los conflictos y reformas que se desarrollaron expresan los componentes y el dinamismo de la estructura social cuando se juega drásticamente la reproducción del grupo. Las disputas familiares, las iglesias como unidades sociales significativas, los diferentes modos de construir liderazgos y las diferencias generacionales tensan y conjugan la estructura de relaciones en que se desenvuelve la vida del grupo. Delimitar esas formas estructurales permitirá conocer dinámicas procesuales de una situación de crisis y ponderar en rigor qué cambia y qué permanece cuando en el campo de estudios de la religión contemporáneo se habla de radicales transformaciones epocales.; This article attempts to make sense of the stresses and directions of changes that occurred after the death of two leaders of an evangelical group. It argues that the conflicts and reforms derived from that conjuncture, in which the reproduction of the group was destabilized, reveal the components and dynamics of the social structure. Family disputes, the churches as significant social units, the different ways to build leadership and generational differences tighten and combine the structure of relationships unfolded by the Free Brethren. A goal of this article is to delineate the structural forms revealed in the procedural dynamics of a reproduction crisis. In the present field of studies of contemporary religion a common attitude of knowledge is to see radical transformation everywhere. Counterbalancing this tendency, my empirical work seek to comprehend what changes and what remains, or the limits unto which a religious or social group can move from traditional culture and social cores.

Austrochthamalia teyucuarensis (Apocynaceae: Asclepiadoideae), una nueva especie endémica de Misiones, Argentina Keller, Hector Alejandro Se describe e ilustra una nueva especie de Austrochthamalia (Apocynaceae), endémica del paraje Teyú Cuaré, Misiones, Argentina. Es el primer representante del género que se documenta para el país y ocupa una posición más austral que el resto de las especies conocidas hasta el presente.

New records of Cyathea atrovirens (Langsd. & Fisch.) Domin (Cyatheaceae) from Argentina Krauckzuk, Ernesto R.; Marquez, Gonzalo Javier; Keller, Hector Alejandro Cyathea atrovirens (Cyatheaceae) is the southernmost tree-fern of Argentina and its presence in the Corrientes province was in doubt after the height of the Yacyretá dam was raised. However, new records confirm the presence of the species in the province and increase the range distribution southward. Comments on the distribution and ecology of the species are now reported.

Hypericum robsonii spec. nova sect. Trigynobrathys (Hypericaceae) from the Misiones Province in Argentina Keller, Hector Alejandro; Crockett, Sara Plant collections conducted in connection with ethnobotanical studies carried out with the Guaraní peoples of Misiones Province, Argentina, resulted in the discovery of a new species of Hypericum L. (Hypericaceae), which is characterized by its epigeous herbaceous scions produced seasonally from subterranean stems or roots. This species, H. robsonii H. A. Keller & S. Crockett, is described and illustrated for the first time. Information about its ecology, habitat and distribution, phenology and ethnobotany is provided.

Enzymatic kinetic resolution of racemic ibuprofen: past, present and future José, Carla; Toledo, Victoria; Briand, Laura Estefania This review is a journey concerning the investigations of the kinetic resolution of racemic ibuprofen for the last 20 years. The relevancy of the pharmacological uses of the S( + ) enantiomer along with its higher cost compared with racemic profen are the driving forces of a variety of scientific research studies addressing the enzymatic resolution of ibuprofen through enantiomeric esterification using lipases as biocatalysts. Lipases of fungal sources such as Candida rugosa, Rhizomucor miehei and the lipase B of Candida antarctica have been extensively studied both in homogeneous and heterogeneous (immobilized on solid supports) processes. In this context, the various alcohols and organic co-solvents frequently used in the esterification of racemic ibuprofen are summarized and discussed in this review. Moreover, recent investigations using membranes as reactors coupled with the separation of the desired product and microfluidic devices are presented. Finally, some guidelines about future perspectives regarding the technology of the kinetic resolution of profens and research niches are given.

Polypropylene nanocomposites produced by in situ grafting of n-butyl acrylate Merchan Sandoval, Julie Pauline; Quinzani, Lidia Maria; Failla, Marcelo Daniel Nanocomposites of polypropylene and organophilic clay are produced by in situ functionalization of PP with n-butyl acrylate(BA) during melt mixing. Three strategies for incorporating materials into the mixer are analyzed and the effect of clay concentrationis evaluated. The materials are examined by FTIR spectroscopy, X-ray diffraction, scanning electron microscopy, differentialscanning calorimetry, thermogravimetry, and rotational rheometry. The results show that all composites prepared in the presence of BAhave similar intercalated clay structure, and that the largest degree of exfoliation is obtained using the sequential mixing technique. Thismethod, which consists in adding the initiator and functionalizing agent to the molten polypropylene followed by the addition of theclay, also produces the largest reduction in molecular weight of the polymer and the largest increase of the elastic modulus. All polymersshow similar crystallization and degradation processes.

Polymorphisms in TNF and IFNG are associated with clinical characteristics of aplastic anemia in Argentinean population Bestach, Yesica Soledad; Sieza, Yamila; Attie, Myriam; Riccheri, Cecilia; Verri, Verónica; Bolesina, Moira; Bengió, Raquel; Larripa, Irene Beatriz; Belli, Carolina Bárbara The impaired hematopoiesis in acquired aplastic anemia (AA) results from immune-mediated mechanisms. We characterized polymorphisms implicated in controlling type-1 cytokine production in 69 patients with AA. Our data suggest that the studied polymorphisms are not associated with susceptibility in the overall AA population. However, the presence of the higher expressing TNF - 308A allele was associated with younger age (p = 0.0297) and more profound neutropenia (p = 0.0312), and over-represented in patients with very severe AA (p = 0.0168). The higher producing IFNG 12 CA-repeat allele showed strong linkage disequilibrium with the + 874T allele, and was associated with a lower hemoglobin level (p = 0.0351). Also, the presence of at least one higher expressing variant was more frequent among patients responding to immunosuppressive treatment (p = 0.0519). Our findings suggest that the presence of higher expressing variants of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in AA patient genotypes could be related to clinical parameters, disease severity and therapy outcomes.

Stimulation of PAR-1 or PAR-4 promotes similar pattern of VEGF and endostatin release and pro-angiogenic responses mediated by human platelets Etulain, Julia; Mena, Hebe Agustina; Negrotto, Soledad; Schattner, Mirta Ana Background: Platelets mediate angiogenesis through the secretion of several factors, including the pro-angiogenic vascular endothelial growth factor (VEGF) and the anti-angiogenic endostatin. Although previous findings indicated that these molecules are packed into different alpha-granules and selectively released by specific stimulation of protease-activated receptor (PAR)-1 or PAR-4, recent evidences are against this hypothesis. Objectives: To elucidate the controversies about the VEGF and endostatin release and the overall angiogenic effect of PARs-stimulated platelets. Methods: VEGF and endostatin were quantified by enzyme linked-immunosorbent assay (ELISA). Endothelial proliferation (pNPP assay), wound healing (scratch assay) and tubule formation (matrigel) of human microvascular endothelial cells (HMEC-1) and endothelial progenitor cells (EPC) were determined using supernatants from PAR-1- or PAR-4-stimulated platelets. Results: Activation of washed platelets (WPs) by PAR-1- or PAR-4-activating peptide (AP) promoted the VEGF and endostatin secretion in a concentration-dependent manner, being PAR-1-AP more potent than PAR-4-AP. The release of both molecules was abrogated by pre-incubation of platelets with PAR antagonists. Activation of platelet-rich plasma (PRP) with either PAR-1-AP or PAR-4-AP induced a significant VEGF secretion. Quantification of platelet-endostatin secretion was not possible in PRP due to the high levels of plasmatic endostatin vs. platelet content. Releasates from PAR-1- or PAR-4-activated WPs promoted similar pattern of angiogenic responses of HMEC-1 or EPC. Moreover, proliferation of HMEC-1 mediated by PAR-stimulated PRP releasates was delayed and significantly lower compared with that induced by PAR-stimulated WPs. Conclusions: Our results are in contrast with the previously described differential release of VEGF and endostatin induced by the selective PAR-1 or PAR-4 stimulation, and support the notion that while circulating endostatin accounts for the maintenance of a systemic anti-angiogenic state, locally, the release of platelet alpha-granule content promotes angiogenesis.

Structural, conformational and vibrational properties of 1,1,1-Trifluoro-N-(1,1,2,2,2-pentafluoroethyl) methanesulfinimidoyl chloride, CF3CF2NS(Cl)CF3 Robles, Norma Lis; Chemes, Doly María; Heinz Oberhammer; Cutin, Edgardo Hugo The structural, conformational, and configurational properties of 1,1,1-Trifluoro-N-(1,1,2,2,2-pentafluoroethyl) methanesulfinimidoyl chloride, CF3CF2NS(Cl)CF3 have been studied by vibrational spectroscopy [IR (vapor) and Raman (liquid)] and quantum chemical calculations [B3LYP, MP2 and B3PW91 levels of theory using the 6-311+G(d), 6-311+G(df) and 6-311+G(2df) basis sets]. According to these theoretical approximations, CF3CF2NS(Cl)CF3 exists in the gas phase as a mixture of a favored anticlinal form (CN bond anticlinal with respect to the CSCl bisector) with C1 symmetry and a less abundant syn conformer showing C1 symmetry as well (ΔG° ≈ 1.20 kcal mol-1). Due to the small contribution only a few corresponding vibrational modes of the syn conformer could be assigned confidently in the experimental spectra. Compared to CF3CF2NS(F)CF3, the replacement of F by Cl produces a clear change in NS bond length and the corresponding stretching frequency, without affecting the conformational properties.

A Steady-State Picture of Solar Wind Acceleration and Charge State Composition Derived from a Global Wave-Driven MHD Model Oran, R.; Landi, E.; Van Der Holst, B.; Lepri, S. T.; Vasquez, Alberto Marcos; Nuevo, Federico Alberto; Frazin, R.; Manchester, W.; Sokolov, I.; Gombosi, T. I. The higher charge states found in slow (<400 km s−1) solar wind streams compared to fast streams have supported the hypothesis that the slow wind originates in closed coronal loops, and released intermittently through reconnection. Here we examine whether a highly ionized slow wind can also form along steady and open magnetic field lines. We model the steady-state solar atmosphere using AWSoM, a global magnetohydrodynamic model driven by Alfv{´e}n waves, and apply an ionization code to calculate the charge state evolution along modeled open field lines. This constitutes the first charge states calculation covering all latitudes in a realistic magnetic field. The ratios $O^{+7}/O^{+6}$ and $C^{+6}/C^{+5}$ are compared to in-situ Ulysses observations, and are found to be higher in the slow wind, as observed; however, they are under-predicted in both wind types. The modeled ion fractions of S, Si, and Fe are used to calculate line-of-sight intensities, which are compared to EIS observations above a coronal hole. The agreement is partial, and suggests that all ionization rates are under-predicted. Assuming the presence of suprathermal electrons improved the agreement with both EIS and Ulysses observations; importantly, the trend of higher ionization in the slow wind was maintained. The results suggest there can be a sub-class of slow wind that is steady and highly ionized. Further analysis shows it originates from coronal hole boundaries (CHB), where the modeled electron density and temperature are higher than inside the hole, leading to faster ionization. This property of CHBs is global, and observationally supported by EUV tomography.

Mitochondrial dysfunction in skeletal muscle during experimental Chagas disease Báez, Alejandra Lidia; Reynoso, Mercedes María Noel; Lo Presti, Maria Silvina; Bazán, Paola Carolina; Strauss, Mariana; Miler, Noemí del Carmen; Pons, Patricia; Rivarola, Hector Walter; Paglini, Patricia Adriana Trypanosoma cruzi invasion and replication in cardiomyocytes and other tissues induce cellular injuries and cytotoxic reactions, with the production of inflammatory cytokines and nitric oxide, both sources of reactive oxygen species. The myocyte response to oxidative stress involves the progression of cellular changes primarily targeting mitochondria. Similar alterations could be taking place in mitochondria from the skeletal muscle; if that is the case, a simple skeletal muscle biopsy would give information about the cardiac energetic production that could be used as a predictor of the chagasic cardiopathy evolution. Therefore, in the present paper we studied skeletal muscle mitochondrial structure and the enzymatic activity of citrate synthase and respiratory chain complexes I to IV (CI–CIV), in Albino Swiss mice infected with T. cruzi, Tulahuen strain and SGO Z12 and Lucky isolates, along the infection. Changes in the mitochondrial structure were detected in 100% of the mitochondria analyzed from the infected groups: they all presented at least 1 significant abnormality such as increase in their matrix or disorganization of their cristae, which are probably related to the enzymatic dysfunction. When we studied the Krebs cycle functionality through the measurement of the specific citrate synthase activity, we found it to be significantly diminished during the acute phase of the infection in Tulahuen and SGO Z12 infected groups with respect to the control one; citrate synthase activity from the Lucky group was significantly increased (p b 0.05). The activity of this enzyme was reduced in all the infected groups during the chronic asymptomatic phase (p b 0.001) and return to normal values (Tulahuen and SGO Z12) or increased its activity (Lucky) by day 365 post-infection (p.i.). When the mitochondrial respiratory chain was analyzed from the acute to the chronic phase of the infection through the measurement of the activity of complexes I to IV, the activity of CI remained similar to control in Tulahuen and Lucky groups, but was significantly augmented in the SGO Z12 one in the acute and chronic phases (p b 0.05). CII increased its activity in Tulahuen and Lucky groups by day 75 p.i. and in SGO Z12 by day 365 p.i. (p b 0.05). CIII showed a similar behavior in the 3 infected groups, remaining similar to control values in the first two stages of the infection and significantly increasing later on (p b 0.0001). CIV showed an increase in its activity in Lucky throughout all stages of infection (p b 0.0001) and an increase in Tulahuen by day 365 days p.i. (p b 0.0001); SGO Z12 on the other hand, showed a decreased CIV activity at the same time. The structural changes in skeletal muscle mitochondria and their altered enzyme activity began in the acute phase of infection, probably modifying the ability of mitochondria to generate energy; these changes were not compensated in the rest of the phases of the infection. Chagas is a systemic disease, which produces not only heart damage but also permanent skeletal muscle alterations.

El regreso de los militares veteranos de guerra de Malvinas a la Armada: entre el ocultamiento, el silencio y el aislamiento. El caso de los marinos del Apostadero Naval Malvinas Rodríguez, Andrea Belén El artículo aborda la inmediata posguerra de los integrantes el Apostadero Naval Malvinas –una unidad logística naval– durante el Conflicto del Atlántico Sur. En concreto, analiza las experiencias de regreso a la Armada de los cabos, suboficiales y oficiales que integraron ese destino, al tiempo que reconstruye las políticas implementadas por la fuerza en la recepción y reinserción de quienes habían combatido. Parte de la hipótesis que en un contexto de profundo desprestigio militar, el silencio, ocultamiento y aislamiento de los veteranos de guerra fue lo que primó al interior de la Marina con el objeto de tratar de combatir los múltiples conflictos que estallaron con la derrota militar, producto no sólo de las responsabilidades de la guerra en las islas sino también de las violaciones a los DD.HH. que las FF.AA. habían cometido en los ‘70.; The article is about the immediate postwar of the sailors that served in the Apostadero Naval Malvinas –a naval logistics unit– during the South Atlantic Conflict. In particular, it analyzes the experiences of return to the Navy of the corporal, non-commissioned officers and officers that had served this destination, while reconstructs the policies implemented by the naval force at the reception and reintegration of those who had fought. This paper is based on the hypothesis that in a context of deep military discredit., silence, secrecy and isolation of war veterans was what prevailed in the Marine in order to try to fight the many conflicts that appeared after the defeat, product not only of the war responsibilities but also of the violations of humans rights that the military had committed in the ‘70s.

El desarrollo y la colonialidad del poder: análisis de dos experiencias agroecológicas de la provincia del Chaco Pegoraro, María Laura Durante los últimos setenta años, el desarrollo como idea fuerza extendió la hegemonía del capitalismo, potenciando la producción y reproducción de sus lógicas en múltiples dimensiones. Oculta así, bajo el signo de la libertad de elegir, de la incorporación social al mundo global y del acceso al bienestar vía el consumo, el proceso de dominación, explotación y conflicto necesario para la imposición del desarrollo a la manera occidental desde el cual fue conceptualizado A pesar de ello, emergen, en el seno mismo del sistema, experiencias alternativas al capitalismo en las cuales el concepto de colonialidad del poder permite identificar las dimensiones que forman parte de dicho proceso de dominación, explotación y conflicto para el desarrollo económico y admite explorar cómo, desde la práctica, los actores adaptan estas dimensiones, las revierten o las superan. En este marco, en este trabajo** indagaremos el proceso productivo, de organización y de interacción en dos cooperativas agroecológicas chaqueñas. Las experiencias concretas, analizadas en una investigación exploratoria cualitativa con anclaje etnográfico, traslucen lógicas alternativas a las capitalistas en algunas dimensiones, pero también similares en otras. La naturaleza no se presenta únicamente como un factor de la producción, siendo el individuo parte de la misma. Lo ambiental se mezcla con lo político, y el conocimiento experto-científico se entrecruza con los saberes de los campesinos-indígenas en la producción. La inserción en el mercado y la definición de los precios carga las ?racionalidades? capitalistas. Las lógicas modernas-capitalistas se cuelan con otras, y se construyen y redefinen. Su importancia radica en que permiten mirar desde un lugar distinto a la modernidad, y reclaman un entendimiento diferente dentro de las ciencias sociales.; Over the past 70 years, the development as a powerful-idea spread out the hegemony of capitalism, promoting the production and reproduction of their logic in multiple dimensions. It hides the process of domination, exploitation and conflict, which is necessary for the imposition of the development on Western stile. It was conceptualized under the sign of freedom of choice, the social inclusion in the global world and the access to welfare by consumption. However, alternative experiences to the capitalism emerge within the system, where the concept of coloniality of power identifies the dimensions that are part of the process of domination, exploitation and conflict for the economic development itself, and admits to explore how actors adapt these dimensions from the practice, reverse them or overcome them. In this framework we will investigate the production process of organization and interaction in two agro-ecological cooperatives in Chaco. The specific experiences, analyzed in a qualitative exploratory research with ethnographically-anchor, show logical alternatives to the capitalist ones in some dimensions, but also similar in others. Nature does not seem to be a production factor, and the human being is part of it. The environment is mixed with politics, and the expert-scientific knowledge intersects with peasant-indigenous knowledge in the production. The fact of inserting in the market and the price definition carries with the capitalists "rationality". The modern-capitalist logics mix with others, and are built and redefined. Its importance lies in that they allow to look from a different place to modernity and demand a different understanding within the social sciences.

¿Pueden las técnicas de mitigación de estrés ambiental mejorar la supervivencia y el crecimiento de plantines de coníferas en el N.O. de la Patagonia? Varela Santiago; Gyenge, Javier Enrique; Diez, Juan Pablo; Weigandt, Mariana Noemi; Caballe, Gonzalo Las etapas iniciales de vida de los árboles son particularmente susceptibles a condiciones de estrés ambiental y eventos extremos. El desarrollo de estrategias de mitigación durante etapas tempranas parece ser un factor determinante para la supervivencia en hábitats con estas condiciones, las cuales, en base a pronósticos climáticos, se predice que serán aún más severas que en la actualidad.

Drugs affecting blood pressure variability: an update Höcht, Christian; del Mauro, Julieta Sofía; Bertera, Facundo Martin; Taira, Carlos Alberto Blood pressure variability (BPV) is considered nowadays a novel risk factor for cardiovascular disease. Clinical evidences support that short-term and long-term BPV independently contribute to target organ damage, cardiovascular events and mortality in patients with hypertension or diabetes. Attenuation of excessive fluctuations of systolic and diastolic BPV has been suggested as an additional therapeutic target in cardiovascular prevention. A growing number of preclinical and clinical studies have focused in the assessment of drug effects or other interventions on the different types of BPV and their contribution in the prevention of cardiovascular events. Prospective clinical trials have shown that antihypertensive classes differ in their ability to control excessive BP fluctuations with an impact in clinical outcomes. Current evidences suggest that calcium channel blockers are more effective than other blood pressure lowering drugs for the reduction of short-term, mid-term and long-term BPV. In order to increase actual knowledge regarding the therapeutic significance of BPV in cardiovascular disease, there is a need for additional clinical studies specifically designed for the study of the relevance of short-term and long-term BPV control by antihypertensive drugs

Exploring the Dynamics of Cell Processes through Simulations of Fluorescence Microscopy Experiments Angiolini, Juan Francisco; Plachta, Nicolas Daniel; Mocskos, Esteban Eduardo; Levi, Valeria Fluorescence correlation spectroscopy (FCS) methods are powerful tools for unveiling the dynamical organization of cells. For simple cases, such as molecules passively moving in a homogeneous media, FCS analysis yields analytical functions that can be fitted to the experimental data to recover the phenomenological rate parameters. Unfortunately, many dynamical processes in cells do not follow these simple models, and in many instances it is not possible to obtain an analytical function through a theoretical analysis of a more complex model. In such cases, experimental analysis can be combined with Monte Carlo simulations to aid in interpretation of the data. In response to this need, we developed a method called FERNET (Fluorescence Emission Recipes and Numerical routines Toolkit) based on Monte Carlo simulations and the MCell-Blender platform, which was designed to treat the reaction-diffusion problem under realistic scenarios. This method enables us to set complex geometries of the simulation space, distribute molecules among different compartments, and define interspecies reactions with selected kinetic constants, diffusion coefficients, and species brightness. We apply this method to simulate single- and multiple-point FCS, photon-counting histogram analysis, raster image correlation spectroscopy, and two-color fluorescence cross-correlation spectroscopy. We believe that this new program could be very useful for predicting and understanding the output of fluorescence microscopy experiments.