Adaptación de la técnica Simple Sequence Length Polymorphism (SSLP) con reactivos locales para el control genético de ratones y ratas endocriadas Torales, Fátima Anabel; Miño, Orlando Samuel; Tau, Rocío Lucía; Ferreccio, Carola Maria; Bachir, Natalia Veronica; Romera, Sonia; Maidana, Silvina Soledad Uno de los problemas a los que se enfrenta la comunidad científica, los bioterios de producción y las empresas biotecnológicas es la autenticación genética de cepas consanguíneas de ratones y ratas utilizadas en la investigación, en producción y control de productos biológicos. Por lo tanto, nos propusimos adaptar, con insumos e infraestructura local, la técnica de SSLP utilizada internacionalmente para el monitoreo genético de animales de experimentación, e implementarla como servicio en Argentina. Seleccionaron 50 microsatelites (MITs) para diferenciar dos cepas de ratones y 26 para discriminar también dos de las cepas de ratas. Las reacciones de PCR para fueron realizadas según el protocolo sugerido por el fabricante de los reactivos, así como también las condiciones de ciclado. La electroforesis en gel de agarosa fue adaptada a las condiciones de nuestro laboratorio y los productos disponibles en nuestro país. Para la estandarización de la técnica se utilizó el ADN de cepas de ratones BALB/c y C57BL/6; y de ratas BN y SD/RiJ cedidas gentilmente por el Dr. Benavides. La validación de la técnica se llevó a cabo por dos operarios distintos, utilizando 2 termocicladores diferentes para evaluar la robustez de la misma. Se lograron amplificar exitosamente los 50 SSLP de ratones y 26 de ratas seleccionados, obteniéndose el patrón de bandas esperado para los controles utilizados. La técnica realizada con ADN de los controles positivos utilizados mostró ser robusta y 100% repetible, obteniéndose resultados idénticos por operarios diferentes y en termocicladores diferentes.; One of the problems faced by the scientific community, production farms and biotechnology companies is the genetic authentication of inbred strains of mice and rats used in research, production and control of biological products. Therefore, we set out to adapt, with local inputs and infrastructure, the SSLP technique used internationally for genetic monitoring of experimental animals, and implement it as a service in Argentina. They selected 50 microsatellites (MITs) to differentiate two strains of mice and 26 to also discriminate two of the strains of rats. The PCR reactions were performed according to the protocol suggested by the reagent manufacturer, as well as the cycling conditions. Agarose gel electrophoresis was adapted to the conditions of our laboratory and the products available in our country. For the standardization of the technique, the DNA of BALB / c and C57BL / 6 strains of mice was used; and BN and SD / RiJ rats kindly provided by Dr. Benavides. Two different operators, using two different thermal cyclers to evaluate its robustness, carried out the validation of the technique. The 50 SSLP of mice and 26 of rats were successfully amplified, obtaining the expected band pattern for the controls used. The technique carried out with DNA from the positive controls used proved to be robust and 100% repeatable, obtaining identical results by different operators and in different thermal cyclers.

Genome-wide analysis uncovers tomato leaf lncRNAs transcriptionally active upon Pseudomonas syringae pv. tomato challenge Rosli, Hernan Guillermo; Sirvent, Emilia; Bekier, Florencia Nicole; Ramos, Romina Nahir; Pombo, Marina Alejandra Plants rely on (in)direct detection of bacterial pathogens through plasma membrane-localized and intracellular receptor proteins. Surface pattern-recognition receptors (PRRs) participate in the detection of microbe-associated molecular patterns (MAMPs) and are required for the activation of pattern-triggered immunity (PTI). Pathogenic bacteria, such as Pseudomonas syringae pv. tomato (Pst) deploys ~ 30 effector proteins into the plant cell that contribute to pathogenicity. Resistant plants are capable of detecting the presence or activity of effectors and mount another response termed effector-triggered immunity (ETI). In order to investigate the involvement of tomato’s long non-coding RNAs (lncRNAs) in the immune response against Pst, we used RNA-seq data to predict and characterize those that are transcriptionally active in leaves challenged with a large set of treatments. Our prediction strategy was validated by sequence comparison with tomato lncRNAs described in previous works and by an alternative approach (RT-qPCR). Early PTI (30 min), late PTI (6 h) and ETI (6 h) differentially expressed (DE) lncRNAs were identified and used to perform a co-expression analysis including neighboring (± 100 kb) DE protein-coding genes. Some of the described networks could represent key regulatory mechanisms of photosynthesis, PRR abundance at the cell surface and mitigation of oxidative stress, associated to tomato-Pst pathosystem.

Percepciones de estudiantes sobre compromiso académico y clima del aula: tendencias pasadas, presentes y futuras sobre una educación superior en transición Rigo, Daiana Yamila El artículo presenta dos estudios, uno desarrollado en el año 2019 y otro en el 2020, que tuvieron como objetivo general describir la percepción de estudiantes de nivel superior de educación sobre el clima de clase -presencial y virtual- y el compromiso académico antes y durante la pandemia. En total participaron 211 estudiantes argentinos, 102 del primer estudio con una edad media de 20,55 años (SD= 3,7), y 109 del segundo estudio, con un promedio de 22,3 años (SD=4,1). La investigación se desarrolló bajo una metodología mixta, utilizando para la recolección de datos tres instrumentos, dos estandarizados, para valorar clima de la clase y compromiso académico, y el otro de preguntas abiertas. Los resultados encontrados, en términos generales, mostraron diferencias estadísticamente significativas en la comparación por año, especialmente en todas las dimensiones del compromiso académico y del clima del aula, a excepción de equipamiento, a favor de la educación en pandemia. Asimismo, dan cuenta de la importancia de expandir los contextos educativos, promover la agencia y repensar la evaluación. Concluyendo sobre la importancia de pensar una educación en transición que valore aspectos positivos de la presencialidad y la virtualidad

Periodic bilayer organization in the complexes of Beta-2 Glycoprotein I with anionic lipid membranes Oliveira, Rafael Gustavo; Paolorossi Nucci, Mariana; Cavalcanti, Leide Passos; Malfatti Gasperini, Antonio; Montich, Guillermo Gabriel β2 glycoprotein I (β2GPI) is a soluble protein that participates in blood coagulation, clearance of apoptotic bodies and generation of antigens in antiphospholipid syndrome among many other functions. We studied the aggregates formed by β2GPI with the anionic phospholipids palmitoyloleoylphosphatidyl glycerol, dimyristoylphosphatidyl glycerol, dipalmitoylphosphatidyl glycerol and cardiolipin using small angle X-ray scattering. The complexes obtained in a medium containing 0.01 M NaCl showed Bragg peaks up to the sixth order in a well-defined integer sequence indicating the presence of a lamellar stacking with a periodicity of 17.8 nm and with largely reduced membrane fluctuations. Modeling the complex signal allowed us to conclude that the coherence length was only two bilayers and that about 15% of the total surface was actually stacked. The space between bilayers allows accommodating an extended β2GPI molecule making a bridge between the interacting bilayers. The interactions between membranes mediated by β2GPI was favored when the membranes were in the liquid crystalline state.

Effect of Hydroponic Green Forage Supplementation during Prepartum and Lactation on Sow and Litter Performances Velásquez Amores, Soledad; Breininger, Elizabeth; Lorenzo Smirnoff, Alejandra; Caggiano, Nicolás; De Simone, Emilio; Acerbo, Marcelo Carlos; De Luca Sarobe, Verónica The incorporation of fibre in the diet of pregnant sows improves the performance dur-ing pregnancy and increases voluntary consumption during lactation. Hydroponic green forage (HGF) constitutes a method of cultivation without soil in controlled environmental conditions. The aim of this study was to evaluate the impact of the supplementation with HGF in lactating sows on productiveperformance and to investigate the metabolic state through the analysis of different biochemical parameters. Twelve sows of the Porcine Productive Unit of the Faculty of Veterinary Sciences were randomly assigned to 2 feeding groups: control (conventional diet) or HGF (hydroponic green forage diet, conventional diet supplemented with HGF) from 7 days prepartum until day 28 postpartum. Productive parameters in sows (weaning-to-oestrus interval and back fat thickness) and in the litter (number of piglets born and weaned, and piglet weight) were evaluated. The effect of the diet in the metabolism of the sows was evaluated by biochemical parameters (total plasma proteins, albumin, glucose, urea and creatinine). Supplementation with HGF did not significantly affect the litter size nor productive parameters but produced a higher weight of piglets at day 60. Although lactation affected some biochemical parameters, no substantial negative consequences of the HGF supplemented diet were observed. Our results suggest that the use of HGF could be an option to take into account in porcine production with economic and environmental benefits.

electroMicroTransport v2107: Open-source toolbox for paper-based electromigrative separations Gerlero, Gabriel Santiago; Marquez Damian, Santiago; Kler, Pablo Alejandro Paper-based electromigrative separations have recently gained relevance due to the rise of paper- based microfluidic devices and their combination with electrophoretic methods, used for many different analytical applications [1]. A new version of the open source toolbox electroMicroTransport for the numerical solution of electromigrative separations is presented, now featuring support for porous substrates, like paper, nitrocellulose and other materials used in paper-based microfluidics. This new version is based on a novel mathematical model for these phenomena recently published by our group. Similar to its previous versions, the toolbox was implemented using OpenFOAM®, meaning that it features native 3D problem handling, support for parallel computation, and a GNU GPL license. This new version of electroMicroTransport includes full support for electroosmotic flow and the novels mechanical and electrical dispersion effects. It is now integrated with a well-recognized electrolyte database with its own management utility, and also includes a renewed algorithm for computing and controlling the electric current drainage in arbitrary surfaces. Moreover, for the first time electroMicroTransport is available for installation as a Docker image, which means that it is able to correctly run on any operating system. Finally, new tutorial examples and a user manual are provided. This new version of electroMicroTransport will enable efficient and reliable numerical prototypes of paper-based electromigrative separations to boost the continuous growth of paper-based microfluidics.

Tetracycline Derivatives Inhibit Plasmodial Cysteine Protease Falcipain-2 through Binding to a Distal Allosteric Site Hernández González, Jorge Enrique; Alberca, Lucas Nicolás; Masforrol González, Yordanka; Reyes Acosta, Osvaldo; Talevi, Alan; Salas Sarduy, Emir Allosteric inhibitors regulate enzyme activity from remote and usually specific pockets. As they promise an avenue for less toxic and safer drugs, the identification and characterization of allosteric inhibitors has gained great academic and biomedical interest in recent years. Research on falcipain-2 (FP-2), the major papain-like cysteine hemoglobinase of Plasmodium falciparum, might benefit from this strategy to overcome the low selectivity against human cathepsins shown by active site-directed inhibitors. Encouraged by our previous finding that methacycline inhibits FP-2 noncompetitively, here we assessed other five tetracycline derivatives against this target and characterized their inhibition mechanism. As previously shown for methacycline, tetracycline derivatives inhibited FP-2 in a noncompetitive fashion, with Ki values ranging from 121 to 190 μM. A possible binding to the S′ side of the FP-2 active site, similar to that described by X-ray crystallography (PDB: 6SSZ) for the noncompetitive inhibitor E-chalcone 48 (EC48), was experimentally discarded by kinetic analysis using a large peptidyl substrate spanning the whole active site. By combining lengthy molecular dynamics (MD) simulations that allowed methacycline to diffuse from solution to different FP-2 surface regions and free energy calculations, we predicted the most likely binding mode of the ligand. Of note, the proposed binding pose explains the low differences in Ki values observed for the tested tetracycline derivatives and the calculated binding free energies match the experimental values. Overall, this study has implications for the design of novel allosteric inhibitors against FP-2 and sets the basis for further optimization of the tetracycline scaffold to produce more potent and selective inhibitors.

Electrical behaviour and evolutionary computation in thin films of bovine brain microtubules Vissol Gaudin, Eléonore; Pearson, Chris; Groves, Chris; Zeze, Dagou A.; Cantiello, Horacio Fabio; Cantero, Maria del Rocio; Petty, Michael C. We report on the electrical behaviour of thin films of bovine brain microtubules (MTs). For samples in both their dried and hydrated states, the measured currents reveal a power law dependence on the applied DC voltage. We attribute this to the injection of space-charge from the metallic electrode(s). The MTs are thought to form a complex electrical network, which can be manipulated with an applied voltage. This feature has been exploited to undertake some experiments on the use of the MT mesh as a medium for computation. We show that it is possible to evolve MT films into binary classifiers following an evolution in materio approach. The accuracy of the system is, on average, similar to that of early carbon nanotube classifiers developed using the same methodology.

Dino on the menu: Tooth traces in a sauropod epiphysis from the Presidente Prudente Formation (Campanian-Maastrichtian), Bauru Group, Brazil - palaeobiological and palaeoecological implications Gomes da Costa Pereira, Paulo Victor Luiz; Pedro Bogado, Joaquin; Baptista Ribeiro, Theo; Paiva Belfort, Leticia; de Valais, Silvina; Roberto dos Anjos Candeiro, Carlos Ichnofossils are a useful tool in determining some aspects of the palaeoecology from past ecosystems, being often used as evidence of interactions between extinct organisms. In this paper we describe tooth traces found in UFRJ-DG 705 R, a fragmentary sauropod epiphysis, from the Presidente Prudente Formation, Bauru Group. We infer that these traces were made by a small to medium-sized crocodyliform, most likely an itasuchid, in a possible act of necrophagy. This new record of tooth traces in the Bauru Group showcases important evidence concerning trophic relations between sauropods and crocodyliforms in this locality during the Late Cretaceous.

Amenaza de extinción de palmares de Butia yatay como resultado del avance de la actividad forestal en Colonia Pando (San Roque, Corrientes); Extinction threat of Butia yatay palm groves as a result of the progress of forestry activities at Colonia Pando (San Roque, Corrientes) Contreras, Félix Ignacio; Baruzzo, Mariana Noemí; Smichowski, Humberto; Milano, Micaela; Contreras, Silvina Andrea La transformación de paisajes naturales en Sudamérica surge como consecuencia del desarrollo humano, relacionado al crecimiento espacial de las ciudades o a la expansión agrícola, ya que ambas implican grandes superficies del territorio y más aún cuando los mismos se encuentran en constante crecimiento. En la provincia de Corrientes, la forestación de Pinus sp. y Eucaliptus sp. es en la actualidad la actividad económica de mayor desarrollo espacial sobre un paisaje local de lomadas arenosas. No obstante, si bien el crecimiento es sumamente significativo, existen muy pocos antecedentes de cómo la actividad forestal impacta sobre este paisaje, principalmente sobre especies autóctonas como los palmares de Butia yatay (Mart.) Becc. En este marco, el objetivo de este trabajo es dar a conocer cómo la expansión de la deforestación pone en riesgo los palmares naturales de Butia yatay, potenciando su extinción si no se regula esta actividad y se protegen especies en peligro. En este trabajo se propone enfatizar los estudios referidos al avance de la actividad forestal, dejando como antecedente la pérdida de uno de los principales componentes fitogeográficos del paisaje.; Landscape transformation of natural landscapes in South America rise up as a consequence of human development, related to the spatial growth of cities or agricultural expansion, since both involve large areas of the territory and even more so when they are constantly growing. In the Corrientes province, Eucalyptus and Pinus forestry. is at the present the economic activity with the greatest spatial development over a local landscape of sandy hills. Although the growth is extremely significant, there is very little previous on how forest activity impacts on this landscape, principally on native species such as the palms groves of Butia yatay (Mart.) Becc. In this context, the aim of this paper is to know how the expansion of forestry activity place the natural palm groves of Butia yatay at significant risk. This activity may bring them to extinction if this activity is not regulated and endangered species are not protected. In this paper it is proposed to emphasize the studies referring to the advancement of forestry activity, leaving as a precedent the loss of one of the main phytogeographic components of the landscape.

In vivo pair correlation microscopy reveals dengue virus capsid protein nucleocytoplasmic bidirectional movement in mammalian infected cells Sallaberry, Ignacio; Luszczak, Alexis; Philipp, Natalia; Costa Navarro, Guadalupe Soledad; Gabriel, Manuela; Gratton, Enrico; Gamarnik, Andrea Vanesa; Estrada, Laura Cecilia Flaviviruses are major human disease-causing pathogens, including dengue virus (DENV), Zika virus, yellow fever virus and others. DENV infects hundreds of millions of people per year around the world, causing a tremendous social and economic burden. DENV capsid (C) protein plays an essential role during genome encapsidation and viral particle formation. It has been previously shown that DENV C enters the nucleus in infected cells. However, whether DENV C protein exhibits nuclear export remains unclear. By spatially cross-correlating different regions of the cell, we investigated DENV C movement across the nuclear envelope during the infection cycle. We observed that transport takes place in both directions and with similar translocation times (in the ms time scale) suggesting a bidirectional movement of both C protein import and export. Furthermore, from the pair cross-correlation functions in cytoplasmic or nuclear regions we found two populations of C molecules in each compartment with fast and slow mobilities. While in the cytoplasm the correlation times were in the 2–6 and 40–110 ms range for the fast and slow mobility populations respectively, in the cell nucleus they were 1–10 and 25–140 ms range, respectively. The fast mobility of DENV C in cytoplasmic and nuclear regions agreed with the diffusion coefficients from Brownian motion previously reported from correlation analysis. These studies provide the first evidence of DENV C shuttling from and to the nucleus in infected cells, opening new venues for antiviral interventions.

Registro de depredación del peludo, Chaetophractus villosus (Desmarest, 1804), sobre un nido de cauquén común, Chloephaga picta , en el noroeste de Santa Cruz Cossa, Natalia Andrea; Gallo, Jorge Alberto; Fasola, Laura; Roesler, Carlos Ignacio; Abba, Agustin Manuel Presentamos el registro de un peludo depredando un nido activo de cauquén común, obtenido pormedio del monitoreo de nidos de esta especie con trampa camara. Los cauquenes no logran defenderel nido, el cual es depredado en su totalidad. Este registro confirma que el peludo es un depredadorde nidos de cauquenes, por lo que su presencia y densidad deberían tenerse en cuenta al momento deplanificar acciones de conservación para estas aves.; We present the record of a large hairy armadillo predating on an active Upland goose nest, obtained by monitoring nests of this species using camera traps. Upland geese were unable to defend the nest, which was entirely preyed upon. This record confirms that the armadillo is an Upland goose nest predator, so its presence and density should be taken into account when planning conservation actions for these birds.

La organización doméstica y las mudanzas de Esteco (1566-1650): Políticas de movilidad; Household and the movements of esteco (1566-1650): Politics of mobility Marschoff, Maria En 1609 los habitantes de Nuestra Señora de Talavera o Esteco –actual provincia de Salta, Argentina- trasladaron su ciudad. En esa coyuntura, algunos de ellos decidieron no participar de la mudanza y migrar hacia otras ciudades de la gobernación del Tucumán. En este artículo, en base al análisis de diversas fuentes documentales, se busca entender el trasfondo de las distintas decisiones que tomaron los habitantes de esta ciudad colonial, en particular la lógica de la elección de trasladarse o permanecer en determinado lugar. De esa manera se han podido trazar vínculos entre los ritmos de movilidad de las personas con el contexto político-económico de la época y de la región, la organización doméstica en la que participaban y los grupos sociales en los que se insertaban en esta sociedad estamental colonial (elite, grupos subalternos, grupos medios).; In 1609 the inhabitants of Nuestra Señora de Talavera or Esteco-nowadays Salta Province, Argentinamoved their city. In this context, several of them decided to migrate to other cities of the Gobernación del Tucumán. Based on the analysis of a wide variety of historical sources, In this work we try to understand the reason of their decisions, in particular why they took the decision either to move or to remain at a certain place. We have been able to link the rhythm of mobility of these people with the political and economical context of that time and region, their households and the social groups (elite, subaltern or middle groups) to which they belonged in this stratum colonial society.

La Scuola Siciliana y sus problemáticas de traducción cultural al español; The Sicilian School: Issues in Cultural Translation into Spanish Gonzalez Molina, Mario Augusto En el presente trabajo se aborda una selección de textos poéticos pertenecientes a la Scuola Siciliana que, en primer lugar, nos permita reflexionar en torno a la problemática de la traducción de una lengua literaria antigua al español moderno. En segundo lugar, la línea de trabajo se basa en indagar la problemática de traducción desde la traslación del léxico específico que nos retrotraiga al código cultural de estos textos. Se tiene en cuenta los conceptos y tópicos propios de la lírica trovadoresca y los temas de la lírica culta castellana para lograr una recomposición del universo cultural medieval una vez llevados al español como lengua de llegada. Por ello el enfoque utilizado proviene del método de la traducción cultural y la adaptación, y elementos de la teoría de la equivalencia para ensayar la reconstrucción del contexto de este corpus, así como las marcas propias del género poético, dejando de lado cuestiones formales como la métrica y la rima. En cuanto al método filológico, la investigación y análisis prescinden de la ortografía del español medieval para adaptarla a los tiempos modernos, tomando solo aquellos términos que puedan responder a la estética y escritura de la Edad Media. Cada texto en lengua original se presenta junto a su traducción para ser tratados de forma espejada y avanzar con el análisis desde el mencionado marco teórico.; This research analyses a selection of lyrical texts belonging to the Sicilian School, which allows us to reflect on translation problems from an ancient literary language into a modern one. The research is based on inquiring about issues arising out of the translation of specific lexicon, and how it would take us to the cultural code of those texts. We consider the concepts and themes of the Troubadours poetry and the Castilian cult poetry to reconstruct the medieval cultural universe once they are translated into Spanish. The study is based on the cultural translation method and the equivalence theory in order to attempt a context reconstruction in this corpus and to reproduce the characteristics of the lyrical genre, disregarding formal issues as metrics and rime. The analysis does not consider the medieval Spanish orthography when adapting it to modern times, considering only specific terms related to aesthetics and writing in the Middle Ages. Each text is presented in the original language alongside its translation to be examined together from the previously mentioned theoretical framework.

Entre lógicas estatales de gestión y demandas indígenas de clasificación: El reconocimiento de los pueblos kolla y diaguita en la Provincia de Salta (1984-2003); Between state management logics and indigenousdemands for classification: the recognition of thekollas and diaguitas peoples in the province of Salta(1984-2003) Sabio Collado, María Victoria; Milana, Maria Paula Este artículo analiza el proceso de reconocimiento formal de los pueblos diaguita y kolla en la Provincia de Salta, cuyas pertenencias indígenas no fueron consideradas por los organismos indigenistas locales sino hasta principios del siglo XXI. Para ello aborda un conjunto de políticas estatales de orden censal y legislativo, en contrapunto con agencias involucradas en procesos organizativos indígenas, tanto en los Valles Calchaquíes como en los Valles Interandinos, en un lapso de dos décadas (1983-2003). Se detiene en un evento de 2003 que habilita la incorporación de representantes de ambos pueblos al Instituto Provincial de Pueblos Indígenas de Salta, marcando una inflexión tanto en las luchas colectivas como en la misma lógica de clasificación y administración de las alteridades del Estado provincial.; This article analyses the formal recognition process of diaguita and kolla people in the Province of Salta, whose indigenous identities had not been considered by local institutions until the beginning of the XXI century. To achieve this, legislative and census policies were approached contrary to agencies involved in indigenous organizational processes in two decades (1983 -2003). It concluded in 2003 with an event that resulted in the representative incorporation of both groups to the indigenous institute of Salta. It was a turning point in both collective struggles and in the logical classification and administration of the otherness of the state.

La Escuela Normal como "Espacio de sociabilidad femenina Salta fines del XIX y mediados del XX; The Normal School as a “Space for female sociability. Salta late nineteenth and mid twentieth Maciel, María Magdalena; Guantay Estrabis, Sofía El trabajo aborda la Escuela Normal “Gral. Manuel Belgrano” a fin de delinear un primer esbozo acerca de la configuración de sociabilidades en Salta hacia fines del siglo XIX, y mediados del XX. Interesa ubicar a la Escuela Normal como espacio de sociabilidad, esto implica considerar que la institución educativa, se posicionó como establecimiento de instrucción donde jóvenes salteñas – en su mayoría- pertenecientes a los círculos de la élite, se formaron, y establecieron vínculos. En este sentido, interesa, rastrear quienes fueron las que accedieron a estos círculos, a que sectores de la sociedad pertenecieron, y cuáles fueron los caminos que recorrieron tras su egreso.; The work addresses the Normal School “Gral. Manuel Belgrano” in order to outline a first sketch about the configuration of sociabilities in Salta towards the end of the 19th century, and the middle of the 20th. It is interesting to locate the Normal School as a space for sociability, this implies considering that the educational institution was positioned as an educational establishment where young people from Salta - mostly - belonging to elite circles, were formed, and established links. In this sense, it is interesting to trace who were the ones who accessed these circles, to which sectors of society they belonged, and what were the paths they traveled after graduation.

La construcción interaccional de espacios de enseñanza-aprendizaje del español/castellano como L2 en contexto indígena; The interactional construction of teaching-learning spaces of Spanish as L2 in an indigenous context Unamuno, Virginia La enseñanza del español como segunda lengua (en adelante, L2) en contexto escolar indígena noestá explícitamente regulada en Argentina. Sin embargo, esto no implica que no existan espaciosen los cuales esta lengua se enseñe a personas que ingresan al sistema escolar sin conocerla oconociéndola fragmentariamente. En este trabajo, a partir de una mirada interaccional de laenseñanza-aprendizaje de segundas lenguas, se analizan diferentes clases con estudiantes wichipara mostrar cómo el español es tratado en las aulas. Por un lado, aulas monogestionadas (dondequien enseña es bilingüe); por otro, aulas bigestionadas (donde enseñan juntas una personabilingüe y una persona monolingüe (en español). El análisis se detiene particularmente enidentificar secuencias discursivas en las cuales el español se constituye en objeto de aprendizaje,contrastando ambos tipos de clases. Sus resultados muestran que, en unas y otras, existenmomentos en que la interacción se detiene para ajustar las relaciones entre repertorios disponibles(en español) y repertorios necesarios para la resolución de la tarea escolar. Sin embargo, lasprimeras clases, en las cuales enseña una persona bilingüe autónomamente, parecieran albergarmás momentos de reflexión metalingüística, así como enunciados bilingües que contextualizan losusos del castellano y permitirían una apropiación más significativa de este.; The teaching of Spanish as an L2 in schools in indigenous contexts is not explicitly regulated in Argentina. However, this does not imply that there are no spaces where Spanish is learnt by people who have partial or no knowledge of it. In this paper, based on an interactional approach to second language teaching and learning, we analyze lessons with Wichi students in order to show how Spanish is treated in their classrooms. To this end, two cases are explored: (i) those in which the teacher is bilingual, and (ii) those in which the lesson is delivered by both a bilingual teacher and a monolingual Spanish-speaking teacher. Concretely, the analysis focuses on identifying discursive sequences in which Spanish constitutes the object of learning, contrasting both types of classrooms. The results show that, in both dynamics/cases, there are moments in which the interaction stops to adjust the relations between available repertoires (in Spanish) and those necessary for the resolution of the school task. Nevertheless, in the lessons taught by autonomous bilingual teachers, there seem to be more instances of metalinguistic reflection and bilingual speech that contextualize the use of Spanish and allow for a more meaningful appropriation of it.

Mar Chiquita y sus localidades, cambios y continuidades en su dinámica demográfica y territorial (1991-2010); Mar Chiquita and its localities, changes and continuities in demographic and territorial dynamics Parracone, Leandro Agustin; Ares, Sofía Estela En el sudeste bonaerense, el partido de Mar Chiquita atraviesa desde hace algunas décadas numerosas transformaciones territoriales. En tal sentido, el objetivo es analizar la dinámica demográfica de las localidades de este distrito entre 1991 y 2010. La metodología es cuantitativa, basada en información de los Censos Nacionales de Población, Hogares y Viviendas. A partir del cálculo de indicadores demográficos se observan en el período 1991-2010 continuidades y rupturas en cuanto al poblamiento de las localidades, dejando en evidencia cambios en cuanto a las formas de habitar. Los poblados situados en el corredor de la Autovía 2 presentan distintos ritmos de crecimiento, en algunos casos negativos, mientras que las ubicadas sobre el corredor de la Ruta Provincial 11 presentan tasas de crecimiento positivas con ritmos acelerados.; In the southeast of Buenos Aires, the Mar Chiquita district has been going through numerous territorial transformations in the last years. In this sense, the objective is to analyze the demographic dynamics of the localities of this district between 1991 and 2010. The methodology is quantitative, based on information from National Population Censuses. The demographic indicators obtained show continuities and breaks in the settlement of the localities and changes in the ways of living. The towns located on Highway No. 2 show different growth rates, in some cases negative, while those located on Provincial Route No. 11 show accelerated growth.

Movilidad urbana y accesibilidad en clave de género: El caso de lxs trabajadorxs de Textilana en el Partido de General Pueyrredon; Urban mobility and accessibility in terms of gender: The case of Textilana workers in the General Pueyrredon District Marioli, Eliana Margarita El objetivo de este trabajo es abordar las características de la industria textil a nivel nacionaly, fundamentalmente, a escala local; más precisamente las condiciones laborales, en tantomovilidad y accesibilidad de lxs trabajadorxs a sus lugares de trabajo. Para ello, se analizaráel caso de la planta de la empresa textil marplatense Textilana. Para dar cuenta de esto, serealizará un mapeo de los domicilios de lxs trabajadorxs, vinculando sus localizaciones con lade la empresa y el trazado del sistema público de transporte urbano colectivo de pasajerxs intrae interurbano del Partido de General Pueyrredón. Con este fin, se utilizará una base de datosde elaboración propia, construida a partir de los expedientes judiciales de los tribunales detrabajo 1 y 2 de la ciudad de Mar del Plata, desde 2007 hasta 2019. El enfoque de este trabajoes interseccional, puesto que el sector analizado cuenta con una amplia presencia de mujeresque se encuentran doblemente vulneradas por su condición de género -mujer- y de clase -trabajadora-.; The goal of this work is to address the characteristics of the textile industry at the national level and, fundamentally, at the local level; more precisely the working conditions, in terms of mobility and accessibility of workers to their workplaces. For this, the case of the plant of the textile company Textilana will be analyzed. To account for this, a mapping of the homes of the workers will be carried out, linking their locations with that of the company and the intra- and inter-urban public transport route of the General Pueyrredón District. To this end, a self-made database will be used, built from the judicial files of Labor Courts 1 and 2 of the city of Mar del Plata, from 2007 to 2019. The focus of this work is intersectional, since the analyzed sector has a large presence of women who are doubly vulnerable due to their gender -women- and class - worker- status.

Quartz chemistry of granitic pegmatites: Implications for classification, genesis and exploration Müller, Axel; Keyser, William; Simmons, William B.; Webber, Karen; Wise, Michael; Beurlen, Hartmut; Garate Olave, Idoia; Roda Robles, Encarnación; Galliski, Miguel Angel Quartz from 254 pegmatites representing eight pegmatite fields and provinces worldwide was investigated by laser-ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS) to determine concentrations of trace elements Al, Ti, Li, Ge, B, Be, Rb, Na, K, Ca, P, Ga, Sb, Zn and U. A total of 271 new analyses combined with 535 published LA-ICP-MS quartz chemistry data were evaluated with binary and ternary trace element discrimination plots and principal component analysis (PCA). The classifications applied for discrimination of pegmatite types include the widely applied NYF(Nb-Y-F) - LCT(Li-Cs-Ta) classification and the new RMG (pegmatites derived from residual melts of granite magmatism) - DPA (pegmatites as direct products of anatexis) grouping. Pegmatites of both classifications can be well distinguished via Al-Ti, Al-Li and Al/Ti-Ge/Ti binary trace element plots and the Ti - Al/10 - 10*Ge ternary diagram. PCA applied to Al, Li, Ti, Be, B, Ge and Rb contents in quartz allowed to further distinguish between anatectic DPA-1 (Li-enriched DPA) and granite-pluton-derived RMG-1 (Li-enriched RMG) pegmatites. Some pegmatite fields and provinces (Hagendorf-Pleystein, Oxford County) are distinguishable by region-specific Li, Ge and Al contents. The results imply that the chemistry of pegmatite quartz is mainly controlled by the origin (source rock chemistry) of pegmatite melts and, to a much lesser extent, by the geodynamic setting of the pegmatite fields and provinces. Chemically primitive NYF and DPA-2 type pegmatites contain quartz with the lowest total trace-element contents and lowest internal-pegmatite trace-element variation, making it potentially suitable for high-tech application. Pegmatite quartz containing >30 μgg-1 Li and >100 μgg-1 Al is strongly indicative of economic spodumene/montebrasite mineralization and, thus, serves as a strong Li-mineralization pathfinder mineral. Quartz with >5 μgg-1 B may be a potential indicator for gem-quality tourmaline mineralization.