Jóvenes e inclusión socio-laboral: reflexiones desde un abordaje multimétodo sobre el Programa Jóvenes con Más y Mejor Trabajo (PJMYMT); Youth and socio-labor inclusion: reflections from a multi-method approach to the Youth with More and Better Work Program (PJMYMT) Ferraris, Sabrina Alejandra; Roberti, María Eugenia Desde principios de la década de 1990 han surgido en Argentina -siguiendo una tendencia latinoamericana- un conjunto de políticas y programas que buscan incidir en la constitución de las trayectorias de los/as jóvenes, con el objetivo de apoyarlos en sus primeras transiciones laborales. En el marco de esta problemática, el propósito del estudio es examinar de qué manera las políticas activas de empleo, que se configuran dentro de un nuevo paradigma de protección social, contribuyen a la inclusión socio-laboral juvenil. Específicamente, se busca comprender la configuración de las trayectorias de jóvenes que participan del PJMYMT (Programa Jóvenes con Más y Mejor Trabajo), con la finalidad de avanzar en una reflexión que permita analizar, desde una aproximación multimétodo en base a estrategias cualitativas y cuantitativas, los recorridos que desarrollan los/as participantes dentro del programa, en particular, sus pasajes por prestaciones que generan vínculos efectivos con el empleo.; Since the beginning of the 1990s, a set of policies and programs have emerged in Argentina -following a Latin American trendthat seek to influence the constitution of the trajectories of young people, with the aim of supporting them in their first labor transitions. Within the framework of this problem, the purpose of the study is to examine how active employment policies, which are configured within a new social protection paradigm, contribute to the socio-labor inclusion of young people. Specifically, it seeks to understand the configuration of the trajectories of young people who participate in the PJMYMT (Youth with More and Better Work Program), with the purpose of advancing in a reflection that allows analyzing, from a multi-method approach with qualitative and quantitative strategies, the routes developed by the participants in the program, in particular, their passages for benefits that generate effective links with employment.

Gestión y conceptualización de las interacciones con el entorno en universidades argentinas; Management and conceptualization of interactions with the environment in Argentine universities Di Bello, Mariana Eva; Romero, Lucia Ana; Soca, Fernanda Andrea; Sanchez, Pablo Gabriel El trabajo analiza de forma comparada concepciones de funcionarios universitarios e instrumentos de gestión de la relación con el entorno comunitario en cuatro universidades nacionales argentinas: Universidad Nacional del Sur (UNS), Universidad Nacional de Mar del Plata (UNMDP), Universidad Nacional de Quilmes (UNQ) y Universidad Nacional Arturo Jauretche (UNAJ). Se utilizan técnicas de revisión de documentos institucionales y análisis interpretativo de entrevistas. Los hallazgos encontrados revelan una jerarquización de espacios organizacionales y la generación de nuevos instrumentos de política y gestión para la interacción con el medio. Se identifican tres modalidades predominantes de interacción con el medio: a) la universidad como sede; b) la participación en polos científico-tecnológicos y c) el establecimiento de nodos.; This work analyses, in a comparative way, university´ staff conceptions and management devices that promote interactions with the community environment in four Argentine national universities: Universidad Nacional del Sur, Universidad Nacional de Mar del Plata, Universidad Nacional de Quilmes and Universidad Nacional Arturo Jauretche. The analytic procedure entails institutional documents review along with interpretive analysis of interviews. Findings reveal a hierarchy of organizational spaces and the generation of new policy and management instruments for the environment interaction. Three main types of universities-socio-economic setting relationships are identified: a) the university as a site; b) the participation on scientific-technological poles and c) the settling of nodes.

Formación profesional y gestión del saber: los casos de la construcción y automotriz en Argentina; Vocational training and management knowledge: the case of construction and automotive in Argentina Granovsky, Pablo; Alfredo, Miguel Angel El siguiente artículo se propone aportar al debate de la política pública en Argentina a partir del papel que desarrolla el actor sindical dentro de la modalidad Formación Profesional. Retomando el desarrollo institucional de la misma, se describe el anclaje territorial y los perfiles profesionales desarrollados para dicha modalidad. En este punto, se busca problematizar sobre las particularidades que la construcción de las políticas de formación profesional asume en contextos de interacción con actores institucionales altamente especializados en los saberes y prácticas propias de las relaciones laborales y actividades sectoriales. Se recupera la experiencia de Formación Profesional gestionada por UOCRA (Unión Obrera de la Construcción de la República Argentina) y SMATA (Sindicato de Mecánicos y Afines del Transporte Automotor). A partir de entrevistas a informantes claves con inserción en la gestión de la Formación Profesional perteneciente al actor sindical y referentes técnicos, el artículo debate con interpretaciones ortodoxas y propone como marco analítico la acción pública y la sectorialización de la formación. El objetivo del artículo es aportar al debate entre el vínculo educación y trabajo a partir de la caracterización de la Formación Profesional como propio de gestión y dentro de un marco político-institucional situado.; The following article is proposed to contribute to the debate of public policy in Argentina based on the role played by the union actor in the Vocational Training modality. Taking up the institutional development of the same, the territorial anchorage and professional profiles developed for this modality are described. The experience of Professional Training managed by UOCRA (Construction Workers Union of the Republic of Argentina) and SMATA (Mechanics and Related Trade Union of Automotive Transport) is recovered. From interviews with key informants with insertion in the management of Vocational Training belonging to the union actor and technical referents, the article discusses with orthodox interpretations and proposes as an analytical framework the public action and the sectorialization of the training. The objective of the article is to contribute to the debate between the education and work link based on the characterization of Vocational Training as its own management and within a situated political-institutional framework.

La violencia sexual es política. Un análisis de Campo de Batalla: Cuerpo de Mujer (Fernando Álvarez, Argentina, 2013) Tornay, Lizel; Alvarez, Victoria La lógica figurativa de los films, más allá de exhibir el mundo, construye sentidos que dialogan y disputan con otros vigentes. Por eso el cine constituye un discurso social privilegiado teniendo en cuenta, además, su capacidad de atravesar distintos niveles de realidad. De tal manera los relatos cinematográficos no solo se presentan como fuentes para el historiador sino también operan como agentes en la construcción de la historia y en su agencia pueden producir intervenciones políticas significativas. Este trabajo se propone analizar el film documental Campo de Batalla. Cuerpo de Mujer (Fernando Álvarez, Argentina, 2013) como agente de sentidos e intervención política. Con este propósito partirá de conceptualizaciones provenientes del campo de los estudios de género, los estudios visuales, aquellos referidos a la construcción de la memoria, los aportes de la historia cultural respecto a las representaciones y la construcción de sensibilidades.; The figurative logic of the films, beyond exhibiting the world, constructs senses that dialogue and dispute with others in force. That is why the cinema constitutes a privileged social discourse, taking into account, in addition, its capacity to cross different levels of reality. In such a way the cinematographic narratives are not only presented as sources for the historian but also operate as agents in the construction of history and in their agency can produce significant political interventions. This work aims to analyze the documentary film Battlefield. Body of Woman as agent of senses and political intervention. With this purpose it will start from , conceptualizations coming from the field of gender studies, visual studies, those referring to the construction of memory, the contributions of cultural history with respect to representations and the construction of sensibilities.; A lógica figurativa dos filmes, além de exibir o mundo, constrói sentidos que dialogam e competem com outros em vigor. Causa disso o cinema constitui um discurso social privilegiado levando em conta, além disso, a sua capacidade de atravessar diferentes níveis de realidade. Desta forma os relatos cinematográficos não só se apresentam como fontes para o historiador mas também operam como agentes na construção da história e na sua agência podem produzir intervenções políticas significativas. Este trabalho visa analisar o documentário Campo de batalha. Corpo de mulher como agente de sentidos e intervenção política. Nesse intuito vai partir de conceitualizações que provem dos estudos de gênero, dos estudos visuais, e daqueles referidos à construção da memória, e da história cultural no que diz respeito às representações e à construção de sensibilidades.

Numerical study of damaged micro-lattice blocks subjected to uniaxial compressive loading Braun, Matias Nicolas; Iváñez, I. This article presents a numerical study on the mechanical behaviour of damaged micro-lattice (ML) blocks submitted to uniaxial compressible loads. The numerical model is implemented in the commercial finite-element code Abaqus/Standard. From the finite-element model, the initial stiffness and the Yield stress of ML blocks are calculated. The damage in ML blocks is modelled as manufacturing defects, that are included in the ML structures using a random algorithm implemented in MatLab. The numerical model is validated with experimental data from uniaxial compression tests carried out on ML intact blocks by other authors. The analysis of the mechanical behaviour of ML blocks is presented in terms of variations of damage percentage, cell type and cell size.

Ráfagas de aire, formas de habitar los sitios de memoria: Muestra de fotografías en el Museo de Arte y Memoria (MAM) de La Plata Jean Jean, Melina; Basso, Florencia El 29 de junio de 2011 se sancionó en Argentina la Ley Nacional 26.691 que declara Sitios de Memoria a los lugares que funcionaron como centros clandestinos de detención, tortura y exterminio (CCDTyE) o donde sucedieron hechos emblemáticos del accionar de la represión ilegal desarrollada durante el terrorismo de Estado ejercido en el paí­s hasta el 10 de diciembre de 1983. Como autoridad de aplicación, la Secretarí­a de Derechos Humanos de la Nación a través de la Dirección Nacional de Sitios de Memoria reglamentó la Ley en el 2014 y se comprometió a garantizar la preservación, señalización y difusión de los sitios, así­ como también su gestión, y la promoción y asistencia de proyectos de nuevos espacios de memoria. De esta forma, se consolidó el trabajo interjurisdiccional que se vení­a desarrollando desde la Red Federal de Sitios de Memoria, creada en el 2006 en la órbita del Archivo Nacional de la Memoria.

On necessary and sufficient conditions for strong hyperbolicity in systems with constraints Abalos, Julio Fernando; Reula, Oscar Alejandro In this work, we study constant-coefficient first order systems of partial differential equations and give necessary and sufficient conditions for those systems to have a well-posed Cauchy problem. In many physical applications, due to the presence of constraints, the number of equations in the PDE system is larger than the number of unknowns, thus the standard Kreiss conditions can not be directly applied to check whether the system admits a well-posed initial value formulation. In this work, we find necessary and sufficient conditions such that there exists a reduced set of equations, of the same dimensionality as the set of unknowns, which satisfy Kreiss conditions and so are well defined and properly behaved evolution equations. We do that by studying the systems using the Kronecker decomposition of matrix pencils and, once the conditions are met, finding specific families of reductions which render the system strongly hyperbolic. We show the power of the theory in some examples: Klein Gordon, the ADM, and the BSSN equations by writing them as first order systems, and studying their Kronecker decomposition and general reductions.

Determining mehlich‐3 and DTPA extractable soil zinc optimum economic threshold for maize Martínez Cuesta, Nicolás; Wyngaard, Nicolás; Sainz Rozas, Hernan Rene; Reussi Calvo, Nahuel Ignacio; Carciochi, Walter Daniel; Eyherabide, Mercedes; Colazo, Juan Cruz; Barraco, Mariano Manuel; Guertal, Elizabeth A.; Barbieri, Pablo Andres Maize (Zea mays L.) is one of the most susceptible crops to zinc (Zn) deficiency. However, in much of the world, soil Zn tests are poorly calibrated, and thus determining a critical soil test, level for Zn is challenging. The objectives of this study were to: (i) produce a field calibration of the Zn Mehlich-3 (M3-Zn) method for predicting maize grain yield response to Zn fertilizer application, (ii) compare the capacity of DTPA extractable Zn (DTPA-Zn) with M3-Zn for predicting the response of maize yield to Zn fertilizer, (iii) determine whether inclusion of soil pH, organic matter (SOM) and extractable phosphorus in a M3-Zn model improves its predictive capacity and (iv) evaluate an economic approach for determining soil Zn thresholds. We conducted 55 field experiments covering a wide range of edaphic and climatic conditions. Maize responded to Zn fertilizer in 29% of the trials. The capacity of M3-Zn and DTPA-Zn to predict relative yield of maize was similar. Inclusion of other soil variables (pH, extractable phosphorus and SOM) did not or only slightly improve the prediction of M3-Zn. Based on the relationship between M3-Zn and DTPA-Zn (R2 = 0.89), one test can be predicted from the other without affecting the calibration. The M3-Zn 'economic threshold' ranged from 0.98 to 2.79 mg kg−1, while for DTPA-Zn it varied from 0.41 to 1.61 mg kg−1. The broad range of economic thresholds shows that differences in maize productivity and grain price between regions and seasons make establishing a single Zn threshold for all economic-productive situations inappropriate.

Magnetic field driven enhancement of the weak decay width of charged pions Coppola, Máximo; Gomez Dumm, Daniel Alberto; Zeballos Noguera, Diego Santiago; Scoccola, Norberto Nerio We study the effect of a uniform magnetic field B~ on the decays π − → l − ν¯l , where l − = e −, µ−, carrying out a general analysis that includes four π − decay constants. Taking the values of these constants from a chiral effective Nambu-Jona-Lasinio (NJL) model, it is seen that the total decay rate gets strongly increased with respect to the B = 0 case, with an enhancement factor ranging from ∼ 10 for eB = 0.1 GeV2 up to ∼ 103 for eB = 1 GeV2 . The ratio between electronic and muonic decays gets also enhanced, reaching a value of about 1 : 2 for eB = 1 GeV2 . In addition, we find that for large B the angular distribution of outgoing antineutrinos shows a significant suppression in the direction of the magnetic field.

Lugares de memoria en la literatura de Laura Alcoba; Lugares de memória na literatura de Laura Alcoba Fit, Rocio Celeste Los lugares de memoria refieren, desde Pierre NORA (1984), a objetos simbólicos que fijan un modelo de representación del pasado mediante procesos dinámicos y plurales. En este trabajo se revisan las principales representaciones sobre la Casa Mariani-Teruggi, una central clandestina de Montoneros en la que fueron desaparecidas seis personas durante la última dictadura militar en Argentina. Laura Alcoba revisita la casa como un lugar de memoria en las fronteras entre la realidad y la ficción, entre el arte y la política. En esa dirección, se focaliza la lectura de La casa de los conejos ([2008] 2010) considerando la centralidad del cronotopos (BAJTÍN 1989) de la casa operativa. Finalmente, se trabajan las vinculaciones trazadas entre esta primera novela, El azul de las abejas (2014) y La danza de la araña (2017), que configuran una trilogía sobre la experiencia de la infancia entre la dictadura y el exilio.; Os lugares da memória se referem, desde Pierre NORA (1984), a objetos simbólicos que fixam um modelo de representação do passado através de processos dinâmicos e plurais. Neste trabalho revisam-se as principais representações sobre a Casa Mariani-Teruggi, uma central clandestina de Montoneros onde desapareceram seis pessoas durante a última ditadura militar na Argentina. Laura Alcoba revisita a casa como um lugar de memória nas fronteiras entre a realidade e a ficção, entre a arte e a política. Nessa direção, se focaliza na leitura de La casa de los conejos ([2008] 2010) considerando a centralidade do cronotopos (BAJTIN 1989) da casa operativa. Finalmente, se trabalham as ligações traçadas entre essa primeira novela, El azul de las abejas (2014) e La danza de la araña (2017), que configuram uma trilogia sobre a experiência da infância entre a ditadura e o exílio.

Fronteras, representaciones y relatos espaciales: La Iglesia Católica Salteña en el caso del culto de Urkupiña en la ciudad de Salta - Argentina; Borders, representations and spatial stories: the salteña catholic church in the case of the Urkupiña cult in the city of Salta- Argentina; Representações e histórias espaciais: a igreja católica salteña no caso do culto Urkupiña na cidade de Salta- Argentina Nava Le Favi, Daniela Andrea El trabajo retoma una mirada interdisciplinaria en el cruce de aportes queprovienen de la semiótica, la comunicación cultura y estudios sobre la religiosidad de vertientes antropológicas, históricas y sociológicas para comprender cómo la Iglesia Católica salteña ejerce el poder de controlar, disciplinar y legislar los espacios del culto de Urkupiña en la ciudad de Salta por ser -además- una devoción alejada de los rituales del canon católico y más cercana al carnaval bajtiniano. Para ello, se intenta explorar una serie de acontecimientos vinculados a la llegada de la Virgen a la ciudad de Salta en el año 2014. Allí, se mira cómo el recorrido que hace el bulto articula un relato espacial desde la visión eclesial lo que habla, de algún modo, sobre cómo la hegemonía piensa que se puede habitar y transitar la ciudad. El trabajo explora la significación de los recorridos, las legislaciones espaciales, las fronteras en un acto de fijación y delimitación de un campo cerrado discursivo donde, de alguna manera, la Iglesia activa los campos de representación de esos espacios e impregna a la devoción con los mismos.; ABSTRACT: The work takes an interdisciplinary look at the intersection of contributions that come from semiotics, cultural communication and studies on the religiosity of anthropological, historical and sociological aspects to understand how the catholic church of Salta exercises the power to control, discipline and legislate spaces of the cult of Urkupiña in the city of Salta for being - in addition - a devotion far from the rituals of the catholic canon and closer to the bajtinian carnival. To do this, an attempt is made to explore a series of events linked to the arrival of the virgin in the city of Salta in 2014. There, we look at how makes articulates a spatial account from the ecclesial vision of what it speaks, somehow, on how the hegemony thinks that the city can be inhabited and transit. The work explores the significance of the routes, the spatial legislation, the borders in an act of fixing and delimiting a closed discursive field where, in some way, the church activates the fields of representation of those spaces and impregnates devotion with the themselves.; O trabalho analisa interdisciplinarmente a interseção de contribuições oriundas da semiótica, da comunicação cultural e de estudos sobre religiosidade a partir de aspectos antropológicos, históricos e sociológicos para entender como a igreja católica de Salta exerce o poder de controlar, disciplinar e legislar espaços do culto de Urkupiña na cidade de Salta por ser - além disso - uma devoção longe dos rituais do cânone católico e mais perto do carnaval bajtiniano. Para isso, é feita uma tentativa de explorar uma série de eventos ligados à chegada da virgem à cidade de Salta em 2014. Aqui, vemos como articula uma conta espacial da visão eclesial do que ele fala, de alguma maneira, como a hegemonia pensa que a cidade pode ser habitada e transitar. O trabalho explora o significado das rotas, a legislação espacial, as fronteiras em um ato de fixar e delimitar um campo discursivo fechado, onde, de alguma forma, a igreja ativa os campos de representação desses espaços e impregna a devoção. Palabras clave: rizoma- representações - histórias espaciais- fronteiras.

In flow metal-enhanced fluorescence for biolabelling and biodetection Gontero, Daniela; Veglia, Alicia Viviana; Bracamonte, Angel Guillermo Escherichia colibacteria were determined by in flow cytometry with laser excitation and fluorescence detection applying ultraluminescent core-shell nanoparticles based on Metal Enhanced Fluorescence (MEF). Core-shell nanoparticles consisted of a 40 nm core modified with a silica spacer grafted with Rhodamine B (RhB). The electromagnetic field in the near field of the core surface enhanced the fluorescence of RhB by plasmonic and fluorophore coupling. The hydrophilic silica spacer allowed the non-covalent interaction with the polarE. colisurface and thus ultraluminescent bacteria biolabelling was developed. Clearly, well defined and bright bacteria imaging was recorded by Laser Fluorescence Microscopy based on the non-covalent deposition of the ultraluminescent nano-emitters. Using these nano-labellers, it was possible to detect labelledE. coliby in flow cytometry. Higher values of Side-scattered light (SSC) and Forward-scattered light (FSC), and number of fluorescent event detections, were observed for labelled bacteria compared to those non-labelled. The sensitivity of the methodology was evaluated by varying bacteria concentration and acceptable analytical figures of merit were determined. Applying this methodology we could quantifyE. colifrom a synthetic real sample of fortified water. Similar results were obtained by bacteria counting with Laser Fluorescence Microscopy and with a cell-bacteria counter.

Neotectonic Activity in the Low-Strain Broken Foreland (Santa Bárbara System) of the North-Western Argentinean Andes (26°S) Arnous, Ahmad; Zeckra, Martin; Venerdini, Agostina Lia; Alvarado, Patricia Monica; Arrowsmith, Ramón; Guillemoteau, Julien; Landgraf, Angela; Gutiérrez, Antonio; Strecker, Manfred R. Uplift in the broken Andean foreland of the Argentine Santa Bárbara System (SBS) is associated with the contractional reactivationof basement anisotropies, similar to those reported from the thick-skinned Cretaceous-Eocene Laramide province of NorthAmerica. Fault scarps, deformed Quaternary deposits and landforms, disrupted drainage patterns, and medium-sizedearthquakes within the SBS suggest that movement along these structures may be a recurring phenomenon, with yet to bedefined repeat intervals and rupture lengths. In contrast to the Subandes thrust belt farther north, where eastward-migratingdeformation has generated a well-defined thrust front, the SBS records spatiotemporally disparate deformation along structuresthat are only known to the first order. We present herein the results of geomorphic desktop analyses, structural fieldobservations, and 2D electrical resistivity tomography and seismic-refraction tomography surveys and an interpretation ofseismic reflection profiles across suspected fault scarps in the sedimentary basins adjacent to the Candelaria Range (CR)basement uplift, in the south-central part of the SBS. Our analysis in the CR piedmont areas reveals consistency between theresults of near-surface electrical resistivity and seismic-refraction tomography surveys, the locations of prominent fault scarps,and structural geometries at greater depth imaged by seismic reflection data. We suggest that this deformation is driven bydeep-seated blind thrusting beneath the CR and associated regional warping, while shortening involving Mesozoic and Cenozoicsedimentary strata in the adjacent basins was accommodated by layer-parallel folding and flexural-slip faults that cut throughQuaternary landforms and deposits at the surface.

Yerba Mate as a Settler Crop: From the Decline of Old-Growth Trees to the Rise of Plantations; La yerba mate como cultivo poblador: Desde la decadencia de los yerbales nativos al auge de los yerbales implantados Zang, Laura Mabel Desde las políticas tendientes a lograr la preservación de los yerbales nativos en la segunda mitad del siglo XIX hasta la difusión de los yerbales implantados a comienzos del siglo XX, el proceso colonizador de la actual provincia argentina de Misiones estuvo estrechamente vinculado a la yerba mate. Después de la Guerra de la Triple Alianza (1865-1870), el Estado nacional comenzó a tener mayor conciencia sobre la ubicación estratégica de Misiones y un mayor conocimiento acerca de las riquezas en recursos naturales que poseía; esta situación desembocó en la creación del Territorio Nacional de Misiones en el año 1881 y en la implementación de políticas estatales tendientes a poblar el espacio a partir del arribo de inmigrantes europeos.; From the policies aimed at preserving old-growth yerba mate trees in the second half of the 19th century to the proliferation of plantations at the beginning of the 20th, the colonization of the present-day Argentine province of Misiones was closely linked to this crop. After the War of the Triple Alliance (1865-1870), the Argentine government became more aware of the strategic location of Misiones and expanded its knowledge of the bountiful natural resources the province possessed. This led to the creation of the National Territory of Misiones in 1881 and the implementation of government policies for colonization of the area by European immigrants.

Control for cooperative transport of a bar-shaped payload with rotorcraft UAVs including a landing stage on mobile robots Gimenez, Javier; Salinas, Lucio Rafael; Gandolfo, Daniel; Rosales, Claudio Dario; Carelli, Ricardo This paper considers the cooperative transport problem of a cable-suspended rigid bar with two rotorcraft UAVs considering collision avoidance, weight distribution, and wind perturbations. The proposal is based on null-space theory and includes a landing stage where the load is settled down on a formation of ground robots. Therefore, a variable formation is considered according to the task phase. In order to simulate the proposal in a realistic environment, very complete dynamic models for UAVs, ground robots, and load are considered. An adaptation stage is incorporated to link the control actions with the inputs required by the dynamic models. Theoretical bounds for the errors are studied under the assumption that perfect velocity tracking is not fulfilled.

Motility and biofilm production involved in the interaction of phosphate solubilizing endophytic strains with peanut, maize and soybean plants Lucero, Cinthia Tamara; Lorda, Graciela Susana; Ludueña, Liliana Mercedes; Anzuay, María Soledad; Taurian, Tania Endophytic plant growth promoting bacteria are of great interest since the ability to colonize the internal tissues of plants gives it an adaptive advantage. To confer any beneficial effect on the plant, bacteria must present a successful colonization and thus be competent in the rhizosphere and finally infect internal tissues. In this sense, bacterial motility is an essential property involved in plant-microorganism interaction where bacteria can colonize the root and at the same time form biofilms. Within plant beneficial bacteria those that present phosphate solubilizing activity are of great interest due to the contribution of phosphorus to plants. The objective of this work was to evaluate the motility and biofilm formation properties of the strains Serratia sp. S119 and Enterobacter sp. J49 growing in the presence of peanut, maize and soybean root exudates. Results obtained indicated that both strains have flagella, possess swimming, swarming and twitching motilities and showed differential chemotactic attraction against root exudates. In addition, the strains under study showed the ability to form biofilms, being this ability greater in minimal media, in which a greater decrease was also seen by the addition of root exudates in the first hours of growth. The changes produced by the addition of root exudates in the chemotaxis and biofilm formation of the strains suggests that compounds released by the plants are detected by these bacteria and could be part of the molecular dialogue involved in their interaction with the roots of plant.

The paradoxical roles of inflammation during PD-1 blockade in cancer Hill, Marcelo; Segovia, Mercedes; Russo, Sofía; Girotti, Maria Romina; Rabinovich, Gabriel Adrián Recent studies have reported paradoxical roles of inflammation in tumor immunity triggered by PD-1 checkpoint antibody (Ab) blockade. Here, we elaborate on this controversy and propose a new perspective that might help understand this paradox. Since inflammatory cytokines and PD-1 blockade are known to target different subsets of exhausted CD8+ T cells, we propose that the timing at which anti-PD-1 Ab therapy and cytokine modulation occur might determine the fate of exhausted CD8+ T cells and perhaps, the clinical outcome of immunotherapeutic modalities.

Synthesis, UV-visible spectroelectrochemistry and theoretical characterization of new polypyridyl Ru(ii) complexes containing 2,4,6-tris(2-pyridyl)-1,3,5-triazine as precursors for water oxidation catalysts Domínguez, Sofía Eugenia; Moran Vieyra, Faustino Eduardo; Fagalde, Florencia In this work, we report the syntheses and physicochemical characterization of new chloro and aqua complexes of Ru(ii) with 2,4,6-tris(2-pyridyl)-1,3,5-triazine (tptz) and 2,2′-bipyridines substituted with donor and acceptor groups in the 4,4′-positions. The aqua complexes behave as precursors for water oxidation catalysts at pH = 1 using Ce(iv) as a sacrificial oxidant. Besides, the oxidized forms Ru(iv) and Ru(v) have been characterized at different pH values by electrochemistry, UV-Visible spectroscopy and spectroelectrochemistry. The reaction mechanisms were studied by combining mixing and stopped-flow experiments with spectrophotometric monitoring in the UV-visible region and all the rate constants were determined together with the corresponding TON and TOF values at pH = 1. Calculations based on Density Functional Theory (DFT and TD-DFT) were performed to support the experimental data.

Genetic Deletion of Galectin-3 Altered the Temporal Evolution of Macrophage Infiltration and Healing Affecting the Cardiac Remodeling and Function After Myocardial Infarction in Mice Cassaglia, Pablo; Penas, Federico Nicolás; Bettazza, Celeste; Fontana Estevez, Florencia Sofía; Miksztowicz, Verónica Julieta; Naya, Nadia Martínez; Llamosas, María Clara; Noli Truant, Sofia; Wilensky, Luciana; Volberg, Verónica; Cevey, Ágata Carolina; Touceda, Vanessa Michelle; Cicale, Eliana Magalí; Berg, Gabriela; Fernández, Marisa Mariel; Goren, Nora Beatriz; Morales, Maria Celina; González, Germán Esteban We studied the role of galectin-3 (Gal-3) in the expression of alternative activation markers (M2) on macrophage, cytokines, and fibrosis through the temporal evolution of healing, ventricular remodeling, and function after myocardial infarction (MI). C57BL/6J and Gal-3 knockout mice (Lgals3-/-) were subjected to permanent coronary ligation or sham. We studied i) mortality, ii) macrophage infiltration and expression of markers of alternative activation, iii) cytokine, iv) matrix metalloproteinase-2 activity, v) fibrosis, and vi) cardiac function and remodeling. At 1 week post-MI, lack of Gal-3 markedly attenuated F4/80+ macrophage infiltration and significantly increased the expression of Mrc1 and Chil1, markers of M2 macrophages at the MI zone. Levels of IL-10, IL-6, and matrix metalloproteinase-2 were significantly increased, whereas tumor necrosis factor-α, transforming growth factor-β, and fibrosis were remarkably attenuated at the infarct zone. In Gal-3 knockout mice, scar thinning ratio, expansion, and cardiac remodeling and function were severely affected from the onset of MI. At 4 weeks post-MI, the natural evolution of fibrosis in Gal-3 knockout mice was also affected. Our results suggest that Gal-3 is essential for wound healing because it regulates the dynamics of macrophage infiltration, proinflammatory and anti-inflammatory cytokine expression, and fibrosis along the temporal evolution of MI in mice. The deficit of Gal-3 affected the dynamics of wound healing, thus aggravating the evolution of remodeling and function.

Male Decapacitation Factor SPINK3 Blocks Membrane Hyperpolarization and Calcium Entry in Mouse Sperm Zalazar, Lucia; Stival, Cintia Estefanía; Nicolli, Anabella Rita; de Blas, Gerardo Andrés; Krapf, Dario; Cesari, Andreina Mammalian sperm acquire ability to fertilize through a process called capacitation, occurring after ejaculation and regulated by both female molecules and male decapacitation factors. Bicarbonate and calcium present in the female reproductive tract trigger capacitation in sperm, leading to acrosomal responsiveness and hyperactivated motility. Male decapacitating factors present in the semen avert premature capacitation, until detached from the sperm surface. However, their mechanism of action remains elusive. Here we describe for the first time the molecular basis for the decapacitating action of the seminal protein SPINK3 in mouse sperm. When present in the capacitating medium, SPINK3 inhibited Src kinase, a modulator of the potassium channel responsible for plasma membrane hyperpolarization. Lack of hyperpolarization affected calcium channels activity, impairing the acquisition of acrosomal responsiveness and blocking hyperactivation. Interestingly, SPINK3 acted only on non-capacitated sperm, as it did not bind to capacitated cells. Binding selectivity allows its decapacitating action only in non-capacitated sperm, without affecting capacitated cells.