Actividades tradicionales y actividades con TIC en la enseñanza y el aprendizaje de la Física: El caso de un estudiante con TDA; Traditional Activities And ICT Activities In The Teaching And Learning Of Physics: A Case Of A Student Whit ADD Domínguez, María Alejandra; Stipcich, Silvia Se comunican avances de una investigación que procura contribuir en el aprendizaje de Física para el caso de un estudiante con TDA. La metodología empleada es el trabajo colaborativo entre diferentes profesionales. Se diseñaron actividades con y sin empleo de simulaciones. Se analiza y compara el tiempo en el que un estudiante con TDA se encuentra en comportamiento físico especial y la cantidad de acciones distractoras. Asimismo se busca conocer el grado de aceptación de las TIC en el aula por el grupo escolar, en especial por el estudiante con TDA y el análisis que realizan de las ventajas y desventajas de tales inclusiones. Los resultados muestran que en la mayoría de las intervenciones que incluyen TIC, en particular, simulaciones integradas en actividades especialmente diseñadas, el estudiante con TDA: disminuye la cantidad de acciones distractoras y el tiempo en el que manifiesta síntomas. El estudiante con el trastorno asume un rol crítico de la implementación. El grupo escolar manifiesta un importante grado de aceptación del uso de TIC en la enseñanza de Física.; This work informs of advances in research that aims to contribute to the learning of Physics in the case of a student with ADD. The employed methodology is that of collaborative work between different professionals. Activities both with and without the use of simulations were designed. The time during which a student with ADD exhibits special physical behaviour is analyzed and compared along with the amount of distracting actions. Additionally, it aims to know to which degree are the ICTs accepted in the classroom by the school group, especially by the student with ADD, and to understand their analysis of the advantages and disadvantages of such approaches. The results show that in most of the interventions involving ICT, in particular those with simulations integrated in especially designed activities, the student with ADD decreases both the amount of distracting actions and the amount of time manifesting symptoms. The student with the disorder takes a critical role in the implementation. The school group shows a high level of acceptance towards the use of ICT in the teaching of Physics.

Uso de TIC’s en el contexto de una enseñanza basada en la investigación; Use of ICT’s in the context of inquiry based learning Gazzola Bascougnet, María Paz; Otero, Maria Rita; Llanos, Viviana Carolina En este trabajo se presentan resultados parciales sobre el uso de TIC’s en el contexto del inquiry based learning, tal como lo propone la Teoría Antropológica de lo Didáctico (TAD). Específicamente nos centramos en el desarrollo de un Recorrido de Estudio e Investigación (REI) codisciplinar. El texto describe el tipo de herramientas utilizadas para colaborar con las actividades de modelización en física y en matemática involucradas en el REI. Se proponen ejemplos sobre cómo fueron utilizadas las TIC’s durante cinco implementaciones realizadas en cursos habituales de la escuela secundaria y se analiza el papel que juegan en una enseñanza basada en el cuestionamiento. Los resultados permiten señalar algunas potencialidades de estas herramientas para ampliar el alcance del estudio así como identificar algunas dificultades de su uso en las aulas.; In this paper we present partial results related to the use of ICT’s in the context of inquiry based learning, as it is proposed by the Anthropological Theory of the Didactic (ATD). Specifically, we focus on developing a co-disciplinary Study and research Path (SRP). The text describes the type of tools used during the implementation to collaborate with the modelling activities in physics and mathematics involved in the RSP. Examples of how ICTs were used during five implementations in regular courses in secondary school are presented, to analyse its role in a study processes based on questioning. The results allow to point out some potentialities of these tools extending the scope of the study as well as to identify some difficulties of its use in the classrooms.

Fotografías de una Identidad Barrial; Photographs of a neighborhood’s identity Funaro, Fernando A partir de una investigación situada en un barrio ferroviario de una ciudad media de la provincia de Buenos Aires, Argentina, se propone un análisis de las relaciones entre los procesos de memoria social, la construcción de identidades urbano-barriales, y la producción y el tratamiento de las imágenes fotográficas. Mediante una investigación en audiovisual, se abordan los usos y la realización de fotografías en las intervenciones críticas de algunos actores sociales referidas a la memoria barrial, que pretenden interpelar narrativas hegemónicas tanto en el plano local como en contextos más amplios. Las imágenes usadas y producidas responden a un conjunto de demandas que reclaman, entre otras, medidas de protección patrimonial en el barrio y la restitución del servicio del tren de pasajeros.; Based on an investigation located in a railway district from an average sized city in the province of Buenos Aires, Argentina, the article suggests an analysis of the relationships between social memory processes and the construction of urban-neighborhood identities, and the production and treatment of photographic images. Through an audiovisual investigation, the uses and the production of photographs are addressed in the critical interventions of some social actors related to the neighborhood history, which intend to interpellate hegemonic narratives both at the local level and concerning broader contexts. Both used and produced images respond to a set of demands that claim, among others, measures of patrimonial protection in the neighborhood and the restitution of the passenger train service.

Step by step evolution of Indeterminate Domain (IDD) transcriptional regulators: From algae to angiosperms Prochetto, Santiago; Reinheimer, Renata Introduction: The Indeterminate Domain (IDD) proteins are a plant specific subclass of C2H2 Zinc Finger transcription factors. Some of these transcription factors play roles in diverse aspects of plant metabolism and development, however the function of most of IDD genes is unknown and the molecular evolution of the subfamily has not been explored in detail. Methods: In this study, we mined available genome sequences of green plants (Viridiplantae) to reconstruct the phylogeny and then described the motifs/expression patterns of IDD genes. Key results: We identified the complete set of IDD genes of 16 Streptophyta genomes. We found that IDD and its sister clade STOP arose by a duplication at the base of Streptophyta. Once on land, the IDD genes duplicated extensively, giving rise to at least ten lineages. Some of these lineages were lost in extant non-vascular plants and gymnosperms, however all of them were retained in angiosperms, duplicating profoundly in dicots and monocots and acquiring, at the same time, surprising heterogeneity in their C-terminal regions and expression patterns. Conclusions: IDD were present in the last common ancestor of Streptophyta. On land, IDD duplicated extensively originating ten lineages. Later, IDD were recruited by angiosperms where they diversified greatly in number; C-terminal and expression patterns accompanying important aspects of plant body evolution.This study provides a solid framework of the orthology relations of green land plant IDD transcription factors, thus increasing the accuracy of ortholog identification in model and non-model species and facilitating the identification of agronomically important genes related to plant metabolism and development.

La cartografía social como posibilidad de reflexión colectiva en contextos de precarización de la vida; Social mapping as a possibility for collective reflection in contexts of precariousness of life; Cartografia social como possibilidade de reflexão coletiva em contextos de precarização da vida Escurra, María Isabel; Rosso, Inés Reflexionar en torno a la construcción de metodologías territorialmente situadas resulta primordial al momento de pensarnos en la práctica extensionista. El Proyecto de Extensión “Nuestro Mapa. Diagnóstico participativo por medio de cartografía social” nos ha obligado a transitar debates que consideramos importantes compartir a la luz de la experiencia y de sabernos parte de un proceso de construcción colectiva de conocimiento que, con sus aciertos y embates, se logró constituir y desarrollar en tramas complejas donde la precarización de la vida y la desciudadanización han adquirido una papel significativo en las últimas décadas. La cartografía social en esta experiencia se ha posicionado como una posibilidad de expresión y transformación en la que la interdisciplina ha cumplido un rol protagónico que es preciso desentrañar. Se presenta como resultado de este proyecto la sistematización envuelta en el desafío que nos propusimos al tornarla también participativa, constituyéndose en la intención de cerrar y de seguir trazando posibles caminos a transitar hacia la reivindicación de derechos en el contexto urbano.; Reflecting on building territorially situated methodologies is essential when thinking about extension practice. The “Nuestro Mapa. Participatory diagnosis through social mapping” Extension Project has forced us to go through discussions that we consider important to share in the light of experience and knowing that we are part of a process of collective construction of knowledge, which, with its successes and mistakes, managed to constitute and develop complex lattices where the precariousness of life and de-citizenization have acquired a significant role in recent decades. Social mapping in this experience has positioned itself as a possibility of expression and transformation in which interdisciplinarity has played a leading role that must be unraveled. The systematization involved in the challenge that we set out to make it also participatory are presented as results of this project, constituting the intention to close and continue tracing possible paths to travel towards the vindication of rights in the urban context.; Refletir sobre a construção de metodologias situadas territorialmente é essencial quando se pensa em prática de extensão. O Projeto de Extensão “Nuestro Mapa. Diagnóstico participativo através da cartografia social” nos forçou a passar por debates que consideramos importantes de compartilhar à luz da experiência e sabendo que fazemos parte de um processo de construção coletiva do conhecimento que, com seus sucessos e erros, conseguiu se constituir e desenvolver em tramas complexas, onde a precarização da vida e a descidadanização adquiriram um papel significativo nas últimas décadas. O mapeamento social nessa experiência se posicionou como uma possibilidade de expressão e transformação na qual a interdisciplinaridade desempenhou um papel principal que é preciso desvendar. A sistematização envolvida no desafio que propusemos tornando-a também participativa é apresentada como resultado deste projeto, constituindo a intenção de fechar e, ao mesmo tempo continuar delineando possíveis caminhos a percorrer na reivindicação de direitos no contexto urbano.

Los medios audiovisuales del centro-sudeste bonaerense en el período 1983-2004; Audiovisual media in the south central area of Buenos Aires Province from 1983 to 2004; Meios audiovisuais do centro da província de Buenos Aires no período 1983-2004 Iturralde, María Eugenia En el presente artículo se analiza la implementación de políticas de comunicación a nivel nacional y su incidencia en ciudades medias del centro-sudeste de la provincia de Buenos Aires. Se tomaron específicamente los casos de Azul, Olavarría y Tandil en el período comprendido desde la asunción de Raúl Alfonsín a la presidencia de la nación (1983) hasta la presentación de los 21 Puntos Básicos por una Comunicación Democrática (2004). La etapa abordada se inscribe en el contexto de profundización de los procesos iniciados con la caída de la matriz estado-céntrica (Cavarozzi, 2002, p. 66), momento en el que desde el plano económico se instauró un sistema de libre mercado que consistió en abrir el comercio interno a la competencia exterior. En lo que respecta a los medios de comunicación, diversos estudios refieren a la existencia de procesos de concentración y extranjerización de la propiedad de estos. El análisis nos deja ver que los medios de las ciudades antes mencionadas siguieron el ritmo sostenido por la re-regulación impulsada a nivel nacional. Las experiencias locales no muestran avances de proyectos sin fines de lucro, sino una fuerte preeminencia de actores del sector privado como prestadores y algunas iniciativas públicas sostenidas por instituciones que se encuentran dentro de la órbita de la Iglesia católica argentina. También se observa el surgimiento de grupos de medios locales, la integración de proyectos radicados en Olavarría a un multimedio de la Ciudad Autónoma de Buenos Aires y la extranjerización de la propiedad de los servicios de cable. En la indagación se optó por una estrategia metodológica de carácter cualitativo. Para la producción de datos se articularon diferentes técnicas y procedimientos: entrevistas abiertas, análisis de documentos y de publicaciones de medios de comunicación consultadas en archivos de las ciudades analizadas. Estas técnicas de producción de información empírica dieron lugar a la interacción y articulación entre datos primarios y datos secundarios.; In this article, we will analyze the implementation of communication policies at the national level and their effect on medium-sized cities in the south central area of Buenos Aires Province. We will specifically look at the cases of Azul, Olavarría, and Tandil during the period stretching from Raúl Alfonsín’s swearing-in as president of Argentina (1983) to the drafting of the 21 Essential Points for Democratic Communication (2004). This era was marked by the entrenchment of processes initiated by the fall of the state-centered matrix (Cavarozzi, 2002, p. 66), which, from an economic perspective, saw the establishment of a free market system that opened domestic trade to foreign competition. In regard to communication media, as various studies have pointed out, their ownership underwent processes of concentration and foreign takeover. Our analysis shows how media outlets in the aforementioned cities kept pace with this nationwide trend towards re-regulation. Local examples reveal no advances in non-profit projects, but rather the prominent role of players from the private sector as service providers and public initiatives backed by institutions in the orbit of the Argentine Catholic Church. We also observed the emergence of local media groups, the integration of projects based in Olavarría to a multimedia company in the Autonomous City of Buenos Aires, and the foreign takeover of cable services. For our study, we opted for a qualitative methodological approach. To produce our data, we combined different techniques and processes: open-ended interviews along with an analysis of documents and publications by different communication media stored in archives in the aforementioned cities. These techniques for empirical information gathering led to the interaction and articulation of primary and secondary sources.; Neste artigo, analisamos a implementação de políticas de comunicação em cidades do Centro da província de Buenos Aires, Argentina; os casos de Azul, Olavarría e Tandil foram tomados especificamente no período desde que Raúl Alfonsín teve assumido até a presidência da Nação - em 1983 - até a apresentação dos 21 Pontos Básicos pra uma Comunicação Democrática - no ano 2004. A etapa analisada faz parte do contexto de aprofundamento dos processos iniciados com a queda da matriz centrada no Estado, quando desde o nível econômico foi estabelecido um sistema de livre mercado que consistia em abrir o comércio interno à concorrência estrangeira. No caso da mídia, vários estudos referem-se à existência de processos de concentração e estrangeirização de sua propriedade. No caso de experiências locais registradas, elas mostram uma forte proeminência de atores do setor privado como fornecedores e algumas iniciativas públicas sustentadas por instituições que estão dentro da órbita da Igreja Católica Argentina. Na análise, foi escolhida uma estratégia metodológica qualitativa. Para a produção dos dados, foram articuladas diferentes técnicas e procedimentos: entrevistas abertas, análise de documentos e publicações na mídia, consultadas em arquivos das cidades analisadas. Essas técnicas empíricas de produção de informações, resultaram na interação e articulação entre dados primários e secundários.

Paracetamol removal by photo-Fenton processes at near-neutral pH using a solar simulator: Optimization by D-optimal experimental design and toxicity evaluation Giménez, Bárbara Natalí; Conte, Leandro Oscar; Alfano, Orlando Mario; Schenone, Agustina Violeta This study discusses the degradation of paracetamol (PCT) by Fenton and photo-Fenton reactions at circumneutral pH with the aid of ferrioxalate and a solar simulator as the light source. The influence of three main operating variables: H2O2 concentration (HP), temperature (T) and radiation level (Rad) on PCT conversion was evaluated with a D-optimal design and a response surface methodology (RSM). The analysis of variance (ANOVA) revealed that Rad and T significantly affected the studied response. The model performance was satisfactory, giving low standard deviation (0.74) and good R2 and adjusted R2 values (0.9997 and 0.9993, respectively). The optimal conditions found for each radiation level were tested. The obtained PCT conversions after 90 min of reaction (75.52 %, 96.88 % and 91.50 %, respectively) were in good agreement with the predicted values (71.28 %, 96.75 % and 92.75 %, respectively). It was observed that the maximum and minimum levels of toxicity (bioluminescence inhibition of Vibrio fischeri bacteria) found in the system were closely related to the maximum and minimum concentrations observed for the reaction intermediaries (hydroquinone and 1,4-benzoquinone). In addition, it was only possible to completely reduce the toxicity of the system after 240 min of reaction when high levels of radiation were applied.

Seasonal variations of Diptera assemblages in urban green patches of Cordoba city, Argentina: Same richness, different composition; Variaciones estacionales de los ensambles de diptera en espacios verdes urbanos de la ciudad de córdoba, argentina: Misma riqueza, distinta composición Montes, Marcela; Silvetti, Luna Emilce; Ferreyra, Mariana; Molina, Silvia Itatí; Díaz, Florentina; Argañaraz, Carina Inés; Battan Horenstein, Moira; Gleiser, Raquel M. Seasonality can have a direct influence on thestructuring of insect communities due to different preferences or tolerances tothe climatic conditions of the species that compose them. The aimof this study was to evaluate seasonal changes in adult Diptera assemblages ofurban green patches in a southern temperate region. Dipterans were sampled fromvegetation with a G-VAC (aspirator) in urban green patches in Córdoba city,Argentina, during the summer and winter of 2014. Overall, dipterans were moreabundant in summer than in winter but globally, their richness was similar. However,richness per site was significantly lower in the winter, reflecting a heterogeneous distribution and higher family turnover during this season. Family composition and/ortheir relative abundances differed between seasons, reflecting the seasonal segregationof several Diptera families in this temperate region of the southern hemisphere.; La estacionalidad puede tener una influencia directa en la estructuración de las comunidades de insectos debido a las distintas preferencias o tolerancias a las condiciones climáticas de las especies que las componen. El objetivo de este estudio fue evaluar los cambios estacionales en dípteros adultos en espacios verdes urbanos. Los dípteros fueron colectados sobre la vegetación con una G-VAC (aspiradora) en espacios verdes urbanos de la ciudad de Córdoba, Argentina, durante el verano e invierno de 2014. Globalmente, la abundancia de dípteros fue mayor en verano que en invierno, mientras que la riqueza fue similar en ambas estaciones. Sin embargo, la riqueza por sitio fue significativamente menor en invierno, reflejando una distribución más heterogénea y un alto recambio de familias en esta estación. La composición de familias y/o sus abundancias relativas difirieron entre estaciones, reflejando la preferencia estacional de varias familias de Diptera en esta región templada del hemisferio sur.

Morphological and molecular characterization of a hybrid zone between Prosopis alba and P. nigra in the Chaco region of northwestern Argentina Vega, Carmen; Teich, Ingrid; Acosta, María Cristina; López Lauenstein, Diego Alejandro; Verga, Aníbal Ramón; Cosacov Martinez, Andrea The Gran Chaco is the largest dry forest in South America. Oneof the key taxa of this biome is the genus Prosopis (Fabaceae)which contains several economically important species. One ofthe characteristics of this genus is its natural capacity toexchange genetic information among congeneric species,generating zones of high morphological and genetic variabilityat contact points. The study and management of these contactareas can contribute to the dynamic conservation of thesenative genetic resources. In this study, we analysed three siteswithin a gradient of land aridity, where P. nigra and P. alba growin sympatry and apparently hybridize. This is the first studyperformed in both, Prosopis pure sites and hybrid swarm,which includes a spatially explicit approach and simultaneouslyanalyses fruit and leaf morphological variation, togetherwith molecular information (microsatellites markers). We performeda multivariate morphological analysis, Bayesian geneticclustering and multivariate associations between geneticand morphological variability in the pure parental populationsand in the hybrid swarm, considering possible spatial autocorrelation.Bayesian cluster analysis revealed two distinct groupscorresponding to each species, differentiating the pure morphotypesfrom sites 1 and 2. Site 3, corresponding to the hybridswarm, was composed of pure P. alba and P. nigra trees andhybrid individuals. We found morphological and molecular evidenceof hybridization between P. alba and P. nigra, and detectednovel phenotypes in the hybrid site.

Mineral dust resuspension under vibration: Onset conditions and the role of humidity Balladore, Federico José; Benito, Jesica Gisele; Uñac, Rodolfo Omar; Vidales, Ana Maria he vibration of dusty surfaces inevitably causes re-entrainment of particles into the atmosphere. Given that movement of mineral dust particles deposited on a surface begins at a critical frequency (fc) and amplitude, an experimental laboratory study was conducted to determine the onset conditions for resuspension of a vibrated granular soil. We determined the resuspension state diagram as a function of frequency and amplitude of a sinusoidal vibration, the granulometry of the dust and the thickness of the soil bed. The mitigation effect of humidity was also evaluated. Critical frequencies ranged between 2.5 and 23 Hz when amplitudes were less than 12 mm. These results were independent of bed thickness and perturbation type. For all particle sizes observed, fc decreased monotonically with A, contrasting with behavior observed for individual particles. In dry samples, fc for large size classes was markedly less when A was greater than 6 mm; while the fc for fine fractions only decreased once amplitudes reached 10 mm. Experiments with wet granular soils demonstrated that wetting above an optimum humidity did not necessarily impede movement and caused agglomeration. This study provides guidelines for managing resuspension of granular soils subjected to vibrations.

La Historia del COVID 19 en tiempos del Coronavirus. Un ensayo inconcluso; The History of COVID 19 in times of the Coronavirus. An unfinished essay Alvarez, Adriana Carlina Este trabajo realiza una mirada histórica por las diversas pandemias de gripe que impactaron enla República Argentina. La Gripe Rusa del siglo XIX, la Gripe de 1918, la influenza asiática de1957, hasta el actual COVID-19.El objetivo es capitalizar los modelos interpretativos utilizados para los estudios de epidemias,con la finalidad de hacer visibles las diferencias con la pandemia del Coronavirus (2020).Dedicamos especial atención a la gripe asiática de 1957/58, tratando de analizar las similitudesy diferencias que las atraviesan, preguntándonos cuán peculiar, terrible o hasta benigna puedeser en comparación con otras de su tipo.; This work takes a historical look at the various influenza pandemics that impacted the Argentine Republic. From the Russian Flu of the XIX century, the Flu of 1918, the Asian influenza of 1957 until the current COVID-19. The objective of this work is to take advantage of the interpretive models used for epidemic studies, in order to contrast previous pandemics with this Coronavirus. Paying special attention to the 1957/58 Asian flu, we analyze the similarities and differences between these pandemics, wondering how peculiar, terrible or even benign can the current one be compared to others of its kind.

Epizootics and sanitary regulations drive long-term changes in fledgling body condition of a threatened vulture Donázar, José Antonio; Cortés Avizanda, Ainara; Ceballos Ruiz, Olga; Arrondo, Eneco; Grande, Juan Manuel; Serrano, David Epizootics and deliberate changes in policies affecting the environment may affect large groups of species and the functioning of entire ecosystems. Although these effects often overlap in time, their simultaneous effect is rarely examined despite their importance as causes of current biodiversity loss. Here, based on the monitoring of an Egyptian vulture (Neophron percnopterus) population over thirty-three years (1986–2018), we increase our knowledge about the effects of anthropogenic-induced changes in food availability, both direct (sanitary policies limiting livestock carcass disposal) and indirect (a wild rabbit epizootic), on brood size and body condition of fledglings at nests. We compared the body mass of fledglings of broods with one chick (Single) and two chicks (within which we distinguished First and Second-hatched). The mass of Second-hatched chicks decreased after the plummet in rabbit populations (in the year 1990) and the regulations limiting carcass disposal (2005), reaching minimum values during the period with lowest food availability (i.e. 2005–2013). Recent sanitary legislation allowing carcass disposal by farmers coincides with a slight recovery in the observed body masses. Overall, this study shows that environmental changes of disparate origin can have synergistic effects on individual condition. Conservation of endangered vultures will require multi-targeted conservation plans aimed at ensuring nutritional requirements, in addition to detailed long-term monitoring, in order to detect obscure/masked drivers that affect body condition of fledglings.

Identificación de genes de resistencia a mancha amarilla en trigo Uranga, Juan Pablo; Schierenbeck, Matías; Perello, Analia Edith; Lohwasser, U.; Simon, M. R. Una de las principales limitantes bióticas que reducen la expresión de la potencialidad de los rendimientos del cultivo de trigo en la Argentina son las enfermedades. La mancha amarilla producida por Pyrenophora tritici-repentis (Died.) Drechs. (anamorph Drechslera tritici-repentis (Died.) Shoem.) es una de las enfermedades más importantes que afectan al trigo. El objetivo de este trabajo fue determinar variabilidad genética para resistencia a ambos patógenos y localizar molecularmente los factores genéticos determinantes de la resistencia frente a estos patógenos, que permitan distinguir progenitores con presencia de alelos favorables para ser utilizados en planes de mejoramiento.

Restoration of antitumor immunity through anti-MICA antibodies elicited with a chimeric protein Torres, Nicolas; Regge, María Victoria; Secchiari, Florencia; Friedrich, Adrián David; Spallanzani, Raúl Germán; Raffo Iraolagoitia, Ximena Lucía; Nuñez, Sol Yanel; Sierra, Jessica Mariel; Ziblat, Andrea; Santilli, Maria Cecilia; Gilio, Nicolas; Almada, Evangelina; Lauché, Constanza Elena; Pardo, Romina Paola; Domaica, Carolina Ines; Fuertes, Mercedes Beatriz; Madauss, Kevin Patrick; Hance, Kenneth W.; Gloger, Israel S.; Zylberman, Vanesa; Goldbaum, Fernando Alberto; Zwirner, Norberto Walter Background Natural killer and cytotoxic CD8+ T cells are major players during antitumor immunity. They express NKG2D, an activating receptor that promotes tumor elimination through recognition of the MHC class I chain-related proteins A and B (MICA and MICB). Both molecules are overexpressed on a great variety of tumors from different tissues, making them attractive targets for immunotherapy. However, tumors shed MICA and MICB, and the soluble forms of both (sMICA and sMICB) mediate tumor-immune escape. Some reports indicate that anti-MICA antibodies (Ab) can promote the restoration of antitumor immunity through the induction of direct antitumor effects (antibody-dependent cell-mediated cytotoxicity, ADCC) and scavenging of sMICA. Therefore, we reasoned that an active induction of anti-MICA Ab with an immunogenic protein might represent a novel therapeutic and prophylactic alternative to restore antitumor immunity. Methods We generated a highly immunogenic chimeric protein (BLS-MICA) consisting of human MICA fused to the lumazine synthase from Brucella spp (BLS) and used it to generate anti-MICA polyclonal Ab (pAb) and to investigate if these anti-MICA Ab can reinstate antitumor immunity in mice using two different mouse tumors engineered to express MICA. We also explored the underlying mechanisms of this expected therapeutic effect. Results Immunization with BLS-MICA and administration of anti-MICA pAb elicited by BLS-MICA significantly delayed the growth of MICA-expressing mouse tumors but not of control tumors. The therapeutic effect of immunization with BLS-MICA included scavenging of sMICA and the anti-MICA Ab-mediated ADCC, promoting heightened intratumoral M1/proinflammatory macrophage and antigen-experienced CD8+ T cell recruitment. Conclusions Immunization with the chimeric protein BLS-MICA constitutes a useful way to actively induce therapeutic anti-MICA pAb that resulted in a reprogramming of the antitumor immune response towards an antitumoral/proinflammatory phenotype. Hence, the BLS-MICA chimeric protein constitutes a novel antitumor vaccine of potential application in patients with MICA-expressing tumors.

Developmentally Programmed Tankyrase Activity Upregulates β-Catenin and Licenses Progression of Embryonic Genome Activation Gambini, Andres; Stein, Paula; Savy, Virginia; Grow, Edward J.; Papas, Brian N.; Zhang, Yingpei; Kenan, Anna C.; Padilla Banks, Elizabeth; Cairns, Bradley R.; Williams, Carmen J. Embryonic genome activation (EGA) is orchestrated by an intrinsic developmental program initiated during oocyte maturation with translation of stored maternal mRNAs. Here, we show that tankyrase, a poly(ADP-ribosyl) polymerase that regulates β-catenin levels, undergoes programmed translation during oocyte maturation and serves an essential role in mouse EGA. Newly translated TNKS triggers proteasomal degradation of axin, reducing targeted destruction of β-catenin and promoting β-catenin-mediated transcription of target genes, including Myc. MYC mediates ribosomal RNA transcription in 2-cell embryos, supporting global protein synthesis. Suppression of tankyrase activity using knockdown or chemical inhibition causes loss of nuclear β-catenin and global reductions in transcription and histone H3 acetylation. Chromatin and transcriptional profiling indicate that development arrests prior to the mid-2-cell stage, mediated in part by reductions in β-catenin and MYC. These findings indicate that post-transcriptional regulation of tankyrase serves as a ligand-independent developmental mechanism for post-translational β-catenin activation and is required to complete EGA.

¿Por qué pensar en la educación en cárceles en contexto de pandemia? Perez, Camila A quienes desde hace años trabajamos defendiendo el derecho a la educación en las cárceles argentinas el aislamiento social preventivo y obligatorio, promulgado el 20 de marzo del 2020, nos ha conmocionado mucho. Dejar de asistir a las cárceles donde trabajábamos semanalmente y pensar cómo vivirían esta situación nuestros estudiantes privados de su libertad modificó nuestra vida cotidiana y nos enfrentó a nuevos desafíos.En este artículo propongo una primera reflexión de un proceso personal pero también colectivo, con el intento de documentar las primeras inquietudes, intervenciones y aprendizajes emergentes. Este material deberá ser analizado cuidadosamente más adelante, cuando esta situación ?excepcional? pase y podamos visualizar su impacto en los diferentes contextos de encierro en los que nos desempeñamos como educadores, militantes e investigadores.

Preocupaciones y demandas frente a Covid-19 : encuesta al personal de salud; Concerns and demands regarding COVID-19. Survey of health personnel Ortiz, Zulma; Antonietti, Laura; Capriati, Alejandro Jose; Ramos, Silvina; Romero, Mariana; Mariani, Javier; Ortiz, Fabián; Pecheny, Mario Martín La pandemia por COVID-19 afectó la organización de los servicios de salud y tuvo consecuencias en los equipos de salud, según las condiciones laborales y de bioseguridad pre-existentes en cada institución. Durante la primera semana de abril de 2020 se realizó un estudio de corte transversal. El objetivo fue indagar acerca de las condiciones que determinan el clima organizacional: liderazgo, comunicación, recursos institucionales, cohesión/gestión de conflictos y capacitación; y cómo éstas eran percibidas por el personal de salud para hacer frente a la pandemia. Se realizaron 5670 encuestas a trabajadores/as y 50 entrevistas a informantes clave de los tres subsectores del sistema de salud (público, privado y de seguridad social). En las encuestas, el 72.9% fueron mujeres, el 51.4% médicos/as y el grupo etario predominante fue el de menores de 40 años. El 47.8% de los/as participantes refirió pluriempleo. En las entrevistas, el 52% fueron varones, el 60% médicos/ as, la edad media 44.8 años. Se estratificaron las dimensiones y se identificaron predictores independientes de percepción: edad, género, tipo de tareas, subsector y jurisdicción. La dimensión percibida con mayor frecuencia como inadecuada fue la de recursos institucionales y la disponibilidad de equipos de protección personal fue identificada como una de las principales preocupaciones. Surgieron demandas de estrategias de contención para el personal de salud y de comunicación institucional clara y uniforme. En conclusión, al momento del estudio el personal de salud percibía serios déficits en sus organizaciones respecto de las condiciones necesarias para enfrentar la pandemia, con diferencias entre subsectores del sistema.; The COVID-19 pandemic affected the organization of health services and had consequences for health teams, according to the pre-existing safety and working conditions. During the first week of April 2020, a cross sectional study was carried out with a qualitative-quantitative approach. The aim was to explore the conditions determining the organizational climate: leadership, communication, institutional resources, cohesion/conflict management, and training; and how these were perceived by health personnel to deal with the pandemic. A total of 5670 healthcare workers participated in an online survey and 50 were interviewed, from all subsectors of the Argentinean health system (public, private and union-health insurance); 72.9% were women, 51.4% were physicians, and the predominant age group was under 40 years. In the qualitative sample (interviews), 52% were men, 62% were physicians, and the average age was 44.8 years. The dimensions of the organizational climate were stratified and five independent predictors of perception of conditions were identified: age, gender, tasks performed, health system subsector, and jurisdiction. The condition most frequently perceived as inadequate were the inaccessibility of institutional resources and the access to personal protective equipment was a major concern. Claims included the need of institutional strategies to support healthcare workers and of a clear and uniform communication. In conclusion, at the time of the study, the health personnel perceived serious deficits in their organizations regarding the conditions necessary to confront COVID-19, with differences among subsectors of the health system.

Simulaciones de dinámica molecular de conductividad térmica entre dos nanopartículas en contacto; Molecular dynamics simulations of thermal conductivity between two particles in contact Mora Barzaga, Geraudys; Miranda, Enrique Nestor; Bringa, Eduardo Marcial The nanoscale properties of materials can have a great influence on their macroscopic behavior; for instance, the generation and accumulationof defects at the nanoscale, such as point defects, porosity, and interfaces, can change their thermal properties. In this work, we studythe role of an interface in the thermal conductivity between two nanoparticles without any external load. We consider a system subjected toa temperature gradient perpendicular to the contact surface and study the thermal conductivity, thermal conductance, thermal resistance,and contact resistance vs nanoparticle size. The thermal resistance at the interface increases linearly with nanoparticles? contact radius ac.A model based on the contact area between two nanoparticles allows us to reasonably explain the obtained numerical results for thethermal conductivity, leading to a net decrease in effective conductivity as the nanoparticle size increases, reasonably well described by a(ac/R) dependence. Simulated thermal conductance was found to be proportional to (ac/R).

Ecosystem services in urban ecological infrastructure in Latin America and the Caribbean: how do they contribute to urban planning? Romero Duque, Luz Piedad; Trilleras, Jenny M.; Castellarini, Fabiana; Quijas, Sandra We developed a conceptual framework that describes the key role of ecosystem services in urban ecological infrastructure. From this framework we analyze how research on ecosystem services has been addressed in cities of Latin America and the Caribbean, in order to discuss their incorporation into policies of urban planning, in the context of nature-based solutions and sustainable development goals. Mexico, Brazil, Chile, Colombia and Argentina represent 90% of research in urban ecosystem services, all of them except Colombia had carried out studies considering green, blue and gray-hybrid infrastructures. However, green-hybrid infrastructure clustered most of the studies. Ecosystem service supply component and intermediate beneficiaries are the most studied. Our results show that most studies have not been developed from the perspective of the biophysical, sociocultural or economic assessment of ecosystem services, on the contrary we recognized or deduced them from proxy variables found within the studies. Our findings suggest that the study of urban ecosystem services in Latin America and the Caribbean is in development and has begun to increase in the last decade. However, we found that the incorporation of urban ecosystem services in urban planning is low, but at the same time, it is in a promising development related to the application of innovative actions such as nature-based solutions and in support of the new global urban agenda.

Continuous and discrete dynamical sampling Díaz Martín, Rocío Patricia; Medri, Ivan Vladimir; Molter, Ursula Maria In this paper we study the continuous dynamical sampling problem at infinite time in a complex Hilbert space H. We find necessary and sufficient conditions on a bounded linear operator A∈B(H) and a set of vectors G⊂H, in order to obtain that {etAg}g∈G,t∈[0,∞) is a semi-continuous frame for H. We study if it is possible to discretize the time variable t and still have a frame for H. We also relate the continuous iteration etA on a set G to the discrete iteration (A′)n on G′ for an adequate operator A′ and set G′⊂H.