Antibody durability at 1 year after Sputnik V vaccination Sanchez, Lautaro Nicolas; Oviedo Rouco, Santiago; Pifano, Marina; Ojeda, Diego Sebastian; Pascuale, Carla Antonela; Mazzitelli, Bianca; Di Diego García, Facundo; González López Ledesma, María Mora; Rodriguez, Pamela Elizabeth; Miglietta, Esteban Alberto; Ceballos, Ana; Rossi, Andrés Hugo; Kreplak, Nicolas; Geffner, Jorge Raúl; Gamarnik, Andrea Vanesa Antibody waning against SARS-CoV-2 over time after vaccination, together with the emergence of new viral variants, pose great challenges for ending the pandemic. To our knowledge, no previous work has assessed the long-term prevalence of anti-SARS-CoV-2 antibodies in individuals vaccinated with Sputnik V (Gam-COVID-Vac).1 We assessed the persistence of anti-spike IgG antibodies and their neutralising capacity against the original SARS-CoV-2 lineage (B.1) and a local isolate of the BA.1 lineage of the omicron (B.1.1.529) variant in a longitudinal cohort during 1 year after Sputnik V vaccination in Argentina. We used 400 paired serum samples (100 samples at each timepoint, including at baseline before vaccination) from 100 volunteers who received two doses of Sputnik V that were obtained between Jan 1, 2021, and Jan 15, 2022. Participants with current or previous SARS-CoV-2 infection, determined by assessing seropositivity to nucleocapsid protein, were excluded from the analysis. The geometric mean (GM) of international units of IgG anti-spike antibodies2 per mL (IU/mL) were 994 (95% CI 769–1285) at 42 days, 80 (60–106) at 180 days, and 36 (27–47) at 360 days after completion of the two-dose vaccination scheme (figure A; appendix p 2). Overall, a 27-fold reduction in IgG was observed 1 year after Sputnik V vaccination.

Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity Guizzo, Melina Garcia; Tirloni, Lucas; González, Sergio Alberto; Farber, Marisa Diana; Braz, Glória; Parizi, Luís Fernando; Dedavid e Silva, Lucas Andre; da Silva Vaz, Itabajara; Oliveira, Pedro L. In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction.

Mejora del secado convencional de zanahoria por aire caliente con pretratamiento de microondas Muñoz, Maria Virginia; Kvapil, Maria Florencia; Iturriaga, Laura Beatriz Se deshidrataron rodajas de zanahoria utilizando dos métodos de secado: aire caliente (SC) (secado convectivo convencional) y microondas como tratamiento previo a la aplicación de aire (SMC). Los tratamientos de secado al aire se realizaron a dos temperaturas diferentes: 60 y 80 °C manteniendo la velocidad de aire constante. Con respecto al tratamiento con microondas se utilizó la máxima potencia del dispositivo (1180 W) hasta alcanzar una pérdida de peso adecuada y luego se concluyó el secado siguiendo las mismas condiciones de secado convectivo. Se obtuvieron las curvas de cinética de secado correspondientes para cada tratamiento aplicado y se compararon. Además, se determinó la rehidratación del producto final como propiedad física. Las rodajas de zanahoria deshidratadas con el tratamiento previo de microondas resultaron con menor contenido de humedad en un menor tiempo y mayor potencial de rehidratación en comparación con las obtenidas mediante deshidratación por convección.; Carrot slices were dehydrated using two drying methods: hot air (SC) (conventional convective drying) and microwave as a pre-treatment to the application of air (SMC). The air drying treatments were carried out at two different temperatures: 60 and 80 °C, keeping the air speed constant. In regard to the microwave treatment, the maximum power of the device (1180 W) was used until an adequate weight loss was achieved and then the drying was concluded following the same convective drying conditions. The appropriate drying kinetic curves were obtained for each applied treatment and compared. In addition, the rehydration of the final product was determined as a physical property. Carrot slices dehydrated with the microwave pre-treatment resulted in lower moisture content in a shorter time and higher rehydration potential compared to those obtained by convection dehydration.

Sliding wear performance of TiAl-based nitride coatings deposited on ADI by cathodic arc deposition and plasma based ion implantation and deposition Colombo, Diego Alejandro; Quintana, Juan Pablo; Mandri, Alejo Daniel; Marquez, Adriana Beatriz; Dommarco, Ricardo This work studied the sliding wear performance of TiAl-based nitride coatings synthesized on austempered ductile iron (ADI) by cathodic arc deposition (CAD) and plasma-based ion implantation and deposition (PBIID). Monolayer CAD TiAlN films and bilayer CAD and PBIID TiAl/TiAlN films deposited on an experimental device were analyzed and benchmarked against a commercial bilayer CAD film. Sliding wear was evaluated in a pin–on–disc tribometer. Two test conditions were employed, one intended to prevent damage (low load, short distance) and another intended to promote damage (higher load, longer distance). Regarding low load tests, all coated samples showed friction coefficients of 0.40–0.45 and negligible wear. Regarding high load tests, all coated variants exhibited lower or equal disc and pin wear rates with respect to uncoated ADI. In addition, coated samples displayed steady-state friction coefficients between 0.2 and 0.6 while uncoated ADI steady-state coefficients between 0.6 and 0.85.

Nutrient dynamics in the Paraná River Delta: Relationship to the hydrologic regime and the floodplain wetlands Primost, Jezabel Elena; Peluso, María Leticia; Sasal, María Carolina; Bonetto, Carlos Alberto South America contains the most extensive freshwater wetlands in the world, associated with the floodplains of the Amazon, Orinoco and Paraná rivers. The Paraná River delta is a large wetland system strongly influenced by the flood/drought cycles of the river. Water quality remains largely unreported at the regional scale. The present study aims to evaluate the effect of increasing anthropogenic disturbances on nutrient concentrations in the Paraná deltaic system. Within this framework, the river-floodplain interaction is examined. Fourteen sites located along 300 km were sampled over the course of three successive years. Most of the studied parameters showed a higher temporal than spatial variability. The temporal variability was associated with flood/drought cycles of the river, while the differences among the sampling sites were related to nearby anthropic activities. Significantly lower nitrate, soluble reactive phosphorus, and suspended matter concentrations occurred in coincidence with high hydrological connectivity, particularly during the El Niño-Southern Oscillation (ENSO) flooding, compared to the medium hydrological connectivity conditions, suggesting the effect of river-floodplain interactions. In contrast, ammonium concentrations were significantly higher after the floods. Also, nitrate concentrations were significantly higher in the upper deltaic stretch than in the middle or lower stretches, despite the loads contributed by several affluents with higher concentrations than those in the Paraná River. The present results demonstrate the extent to which the Paraná River delta removes suspended matter, nitrate, and P, thus providing an enormously valuable ecosystem service by maintaining acceptable water quality in the river despite increasing contaminant loads. This study supplies a detailed baseline of water quality and knowledge about ecosystem functions in the deltaic wetlands, useful in the design of conservation policies for safeguarding the largest floodplain in Argentina.

Crisis and class inequality in Argentina: a new analysis using household survey data Féliz, Mariano; Millon Alderete, Maria Emilia This paper explains how the stagnation and crisis in Argentina (2009–2020) reshaped the standard of living of the working class and how their effects are reflected through inequality indexes. Many studies have used quintile stratification for inequality analysis. Following the traditions of Marxian/structural theoretical frameworks, we analyse inequality by defining the different factions within the working class, estimating the Socio Occupational Condition (CSO) from Census Data. This strategy allows us building different strata within the working classes from information on regular surveys. We work with the data from the Permanent Survey of Households (EPH) of Argentina. We use bootstrapping techniques to strengthen our estimations of mean income, too, in order to improve our analysis of the difference between classes and variance’s income estimator. We analyse the evolution of inequality with several generalized entropy indexes. These indexes allow us to describe the composition of inequality between the different classes and within each class. We complement the analysis with macroeconomic estimates of income distribution to include estimates for income appropriation from other social classes. We study how macroeconomic developments and policies during the recent crisis have affected inequality.

"I did, I did taw a puddy tat!" Pumas in urban ecosystems of Latin America: a review of the mediatic information Guerisoli, Maria de Las Mercedes; Schiaffini, Mauro Ignacio The concentration of people living in small areas has increased in the last decade, with more than half of the world's population living in cities. This is particularly true for Latin America, a region with no particular high contribution to the world total population, but hosts several large cities. The increase in urbanization causes several threats to wildlife that face the loss of their habitat and novel environmental pressures. As the number of wildlife entering cities seems to have increased in the last year, we characterize the temporal and geographical events of a widely distributed carnivore, the puma, Puma concolor. We performed an exhaustive search for media news regarding the sighting, capture, and/or killing of pumas within human settlement areas, and tried to relate them with potential explanatory variables. We found a total of 162 events in Latin America in a period of the last 10 years, particularly concentrated in the year 2020. Most records came from Brazil, followed by Argentina, Chile, and Mexico. Of the total, 41% were only sightings, 58% were captures, and a minor percentage were considered as mascotism. Almost the same number of records came from highly populated areas (cities) than from low populated areas (rural) but with important differences between countries. The countries with more records in urban areas (Brazil and Mexico) showed a larger surface occupied by cities. The countries with most records in rural areas (Argentina and Chile) present the opposite pattern of occupied surface. This might indicate that different percentages of areas dedicated to cities or urban spaces might explain the differences among countries. The most important variable related to puma events in the populated areas was sky brightness, while human density and cattle density explained minor parts. The “anthropause” due to the COVID-19 pandemic might explain the larger number of records from 2020, while the absence of high-quality habitats due to fragmentation and high cattle density, might force the pumas to enter populated areas searching for food. Minor values of night lights could be related to a facilitation of efficiency of foraging behavior. Although some bias might exist in the data, the results should be taken into account as general statements for all analyzed countries.

Pachygenium laurense (Orchidaceae, Spiranthinae), a new orchid species from Argentina—morphological evidence and phylogenetic reconstruction Martín, Claudia Melisa; Morales, Adriana Marisel; Dudek, Magdalena; Szlachetko, Dariusz L. Background. Pachygenium embraces a group of terrestrial species formerly placed in Pelexia sensu lato. The genus currently comprises some 60 species, most of which are known from the southern parts of Brazil and Paraguay, with few species distributed in the Andean countries—only four species have been recorded from Argentina so far. In Jujuy Province, Argentina a new species of Pachygenium was found during our fieldwork. The aim of this article was to provide morphological and molecular evidence for its membership in this genus. Methods. Materials from specimens were collected in the field and examined by classical taxonomic and molecular biological techniques, e.g., PCR and sequencing DNA. Phylogenetic reconstruction was performed by maximum-likelihood and Bayesian inference. Results. Pachygenium laurense from Argentina is described and illustrated based on morphological evidence and its taxonomic position was confirmed by phylogenetic analyses. A new combination for Pachygenium gutturosa is also proposed. A key for identification is provided for the Pachygenium species occurring in Argentina. Conclusion. Pachygenium laurense is the fifth species of the genus recorded from Argentina.

Type-2 Cytokines Promote the Secretion of the Eosinophil–Attractant CCL26 by Intestinal Epithelial Cells in Food-Sensitized Patients Vaccaro, Julián; Canziani, Karina Eva; Guzmán, Luciana; Bernedo, Viviana; García, Marcela; Altamirano, Eugenia Margarita; Feregotti, Emanuel; Curciarello, Renata; Muglia, Cecilia Isabel; Docena, Guillermo Horacio Several inflammatory processes of the bowel are characterized by an accumulation of eosinophils at inflammation sites. The mechanisms that govern mucosal infiltration with eosinophils are not fully understood. In this work, we studied the colorectal polyp-confined tissue containing eosinophils and we hypothesized that intestinal epithelial cells are the cell source of eotaxin-3 or CCL26, a potent chemoattractant for eosinophils. We analyzed colorectal polyps (n=50) from pediatric patients with rectal bleeding by H&E staining and eosin staining, and different pro-inflammatory cytokines were assessed by RT-qPCR and ELISA. IgE and CCL26 were investigated by RT-qPCR, ELISA and confocal microscopy. Finally, the intracellular signaling pathway that mediates the CCL26 production was analyzed using a kinase array and immunoblotting in human intestinal Caco-2 cell line. We found a dense cell agglomeration within the polyps, with a significantly higher frequency of eosinophils than in control adjacent tissue. IL-4 and IL-13 were significantly up-regulated in polyps and CCL26 was elevated in the epithelial compartment. Experiments with Caco-2 cells showed that the type-2 cytokine IL-13 increased STAT3 and STAT6 phosphorylation and eotaxin-3 secretion. The addition of the blocking antibody Dupilumab or the inhibitor Ruxolitinib to the cytokine-stimulated Caco-2 cells diminished the CCL26 secretion to basal levels in a dose-dependent manner. In conclusion, our findings demonstrate a high frequency of eosinophils, and elevated levels of type-2 cytokines and eotaxin-3 in the inflammatory stroma of colorectal polyps from pediatric patients. Polyp epithelial cells showed to be the main cell source of CCL26, and IL-13 was the main trigger of this chemokine through the activation of the STAT3/STAT6/JAK1-2 pathway. We suggest that the epithelial compartment actively participates in the recruitment of eosinophils to the colonic polyp-confined inflammatory environment.

Chalcogen bearing tetrasubstituted zinc (II) phthalocyanines for CT26 colon carcinoma cells photodynamic therapy Ezquerra Riega, Sergio Dario; Valli, Federico; Rodriguez, Hernan Bernardo; Marino, Veronica Julieta; Roguin, Leonor Patricia; Lantaño, Beatriz; Garcia Vior, María Cecilia The synthesis and photochemical properties of six new lipophilic chalcogen tetrasubstituted zinc (II) phthalocyanines and their corresponding water-soluble cationic derivatives were investigated for CT26 colon carcinoma cells photodynamic therapy. Sulfur and selenium phthalocyanines Q-bands are red shifted around 10 nm with respect to the Q-band of oxygen phthalocyanines. The introduction of Se atoms in peripheral position generates non-significant shifts in the absorption spectrum compared to S-bearing phthalocyanine. In addition, Q-band absorption coefficient of selenium phthalocyanines showed to be higher than their respective sulfur and oxygen analogs, which is relevant for dosing in PDT studies. The fluorescence emission maximum wavelength showed the same trend regards to the presence of chalcogen atoms, O < Se < S. All phthalocyanines are efficient singlet oxygen generators with values in the range 0.42–0.75. In most cases, increasing the atomic number of chalcogen atoms produces a rise of singlet oxygen production efficiency. The replacement of O for S or Se atoms cause a red shifting around 20 nm of triplet-triplet maximum absorption wavelength and the introduction of Se atoms causes a significant shortening of τT. The photodynamic effect was evaluated on CT26 colon carcinoma cells. Oxygen phthalocyanines triggered cell death both in the absence or presence of light, whereas sulfur and selenium phthalocyanines diminished cell viability in a concentration dependent manner only after light exposure. S-bearing phthalocyanines showed the best photodynamic efficiency among all novel phthalocyanines.

Comparative study of the dissolution of LCO in HCl medium with and without H2O2 Drajlin Gordón, Diego Sebastián; Suarez, Daniela Silvana; Toro, Norman; Gálvez, Edelmira D.; Pinna, Eliana Guadalupe; Rodriguez, Mario Humberto The dissolution of LiCoO2 (LCO) from spent lithium-ion batteries (LIBs) has been widely studied with organic and inorganic acids. Among these acids, HCl is the one that showed the best results when used at concentrations higher than 4 M. However, its higher cost compared with other acids is disadvantageous. Taking this into account, this work aims to perform a comparative study of the effect of different operational variables such as temperature, reaction time, leaching agent concentration (HCl) and reducing agent concentration (H2O2) on the dissolution efficiency of LCO for the systems HCl and HCl-H2O2 to determine the optimal parameters to achieve a maximum dissolution in minimum time at low temperatures and reagent concentrations. Increasing temperature, time and concentration of the reagents had a positive effect on the dissolution of LCO. When working with HCl 1.8 M, the highest dissolution for LCO, 91.0% was obtained at 348 K for 60 min. Furthermore, a slightly higher oxide dissolution (93.0%) was obtained in a reducing medium at the same temperature in half the time and with a concentration of HCl more than ten times lower. This will allow us to propose an alternative process to the existing ones with economic and ecological advantages.

Lectura colaborativa de tablas de frecuencias: Análisis secuencial del proceso sociocognitivo; Collaborative Reading of Frequency Tables: Sequential Analysis of the Socio-Cognitive Process Castellaro, Mariano Andrés; Roselli, Nestor Daniel El trabajo se inscribe en un programa general de investigación cuyo propósito es trasladar el estudio de la interacción entre pares al ámbito de la apropiación de diferentes sistemas externos de representación. En el presente artículo de investigación científica y tecnológica se analiza el proceso sociocognitivo involucrado en la comprensión colaborativa de una tabla cruzada de frecuencias (en díadas), desde una perspectiva secuencial. Se diferenciaron tres fases: posicionamiento inicial frente a la consigna, elaboración y toma de decisión final. Participaron 120 díadas (240 estudiantes) de 6° y 7° grado, de la ciudad de Rosario y cercanías (Argentina). Las diferenciaciones principales refirieron al momento de elaboración. En general, predominó el acuerdo sobre el conflicto, sin fundamentación; cuando se registraron modalidades fundamentadas, predominaron las unilaterales; en los casos de conflicto, prevaleció su resolución relacional. Estas tendencias se acentuaron o debilitaron según menor o mayor dificultad del ítem. La composición sociocognitiva también registró diferenciaciones significativas, en las fases de elaboración y resolución, oponiendo díadas simétricas avanzadas, respecto a las simétricas básicas y asimétricas. Los resultados condicen con los postulados de la teoría del conflicto sociocognitivo y se corroboran los niveles de comprensión reportados por la literatura.; The work is part of a general research program whose purpose is to transfer the study of interaction between peers to the field of appropriation of different external systems. of representation. Thus, in this scientific and technological research article, the socio-cognitive process involved in the collaborative understanding of a cross table of frequencies (in dyads) is analyzed from a sequential perspective. Three phases were differentiated: initial positioning against the slogan, elaboration and final decision making. 120 dyads (240 students) from 6th and 7th grade, from the city of Rosario and surroundings (Argentina) participated. The main differences referred to the time of preparation. In general, the agreement prevailed over the conflict, without justification; when substantiated modalities were recorded, unilateral ones predominated; in cases of conflict, its relational resolution prevailed. These tendencies were accentuated or weakened according to lesser or greater difficulty of the item. The socio-cognitive composition also registered significant differences in the elaboration and resolution phases, opposing advanced symmetrical dyads, with respect to the basic symmetrical and asymmetrical ones. The results agree with the postulates of the socio-cognitive conflict theory and the levels of comprehension reported by the literature are corroborated.

Marine deposits of the Chuy Formation (Late Pleistocene) and isostatic readjustments in the area of Laguna de Rocha (Uruguay); Depósitos marinos de la Formación Chuy (pleistoceno tardío) y reajustes isostáticos en el área de la Laguna de Rocha (Uruguay); Depósitos marinhos da Formação Chuy (Pleistoceno tardio) e reajustes isostáticos na área da Laguna de Rocha (Uruguai) Castiglioni, Eduardo; Gaucher, Claudio; Perillo, Gerardo Miguel E.; Sial, Alcides The Chuy Formation is characterized by up to 135 m of an alternation of green pelites and fine to coarse sandstones with sparse invertebrate fossils. The marine deposits are interbedded with continental, loessic deposits of the Libertad For-mation and overlain by the Dolores Formation, made up of mudstones with calcareous concretions.The results of the radiocarbon dating showed calibrated ages of 13.9±0.2, 41.5±1.9 and 50±3 ka BP on bioclasts of marine fossils at an elevation (above sea level, asl) of -2.8, -6.13 and -8 m respectively. Such ages and elevations are in disa-greement with the sea-level evolution inferred for the Late Pleistocene. Two possible explanations are: (a) the ages rep-resent minimum ages and deposition took place during the last interglacial at 115-130 ka (Marine Isotope Stage MIS 5e), or (b) they represent depositional ages but the area experimented large isostatic readjustments during and after the last glaciation. Several lines of evidence suggest a Late Pleistocene age for the upper Chuy Formation, including the 18O curve obtained from invertebrate shells, which shows large secular variations consistent with MIS 1 to 7.The proposed scenario envisages significant subsidence between 50-20 ka due to the far-field effects of glacial load in the Andes/Patagonia. At 20 ka the eustatic regression outpaced subsidence, leading to continentalization of the Laguna de Rocha area. Marine conditions returned at 15 ka and into the Holocene, except for continental deposits (Dolores For-mation) at ca. 11-10 ka (Younger Dryas). An uplift of 115 m took place between 15 and 9 ka in the area, which is interpreted as post-glacial rebound. In the Holocene, moderate subsidence was further recorded. A regional trend is observed, with uplift of marine deposits increasing towards the W-SW, which is consistent with an explanation as post-glacial isostatic rebound.; La Formación Chuy se caracteriza por hasta 135 m de una alternancia de pelitas verdes y areniscas finas a gruesas con fósiles de invertebrados marinos. Los depósitos marinos están intercalados con depósitos loéssicos continentales de la Formación Libertad y sobreyacidos por la Formación Dolores, con lodolitas con concreciones calcáreas. Los resultados de dataciones radiocarbónicas mostraron edades calibradas de 13900±200, 41500±1900 y 50000±3000 años AP, para bioclastos de fósiles marinos a una cota sobre el nivel medio del mar (NMM) de -2,8, -6,13 y -8 m, respec-tivamente. Estas edades y cotas están en desacuerdo con la evolución del nivel medio del mar aceptada para el Pleisto-ceno tardío. Dos posibles explicaciones son: (a) las edades representan edades mínimas y la depositación tuvo lugar durante el último interglacial (115-130 ka; MIS 5e), o (b) representan edades de depositación, pero el área experimentó grandes reajustes isostáticos durante y después de la última glaciación. Varias líneas de evidencia favorecen una edad Pleistoceno tardío para la Formación Chuy superior, incluyendo la curva de 18O obtenida para conchillas de invertebra-dos, que muestra grandes variaciones seculares consistentes con los estadios MIS 1 a MIS 7. El escenario propuesto prevé un hundimiento significativo entre 50-20 ka debido a los efectos lejanos de la carga glacial en los Andes/Patagonia. A los 20 ka, la regresión eustática superó al hundimiento, lo que llevó a la continentalización en el área de Laguna de Rocha. Las condiciones marinas regresaron a los 15 ka y durante el Holoceno, excepto por depósitos continentales (Formación Dolores) en 11-10 ka (Younger Dryas). Se produjo un levantamiento de unos 115 m entre 15 y 9 ka en la zona, lo que se interpreta como un rebote post-glacial. En el Holoceno, se registra nuevamente un hundimiento moderado. Se observa una tendencia regional, con un aumento de los depósitos marinos hacia el W-SW, lo que es consistente con un rebote isostático post-glacial.; A Formação Chuy é caracterizada por uma alternância de até 135 m de folhelhos verdes e arenitos finos a grossos com fósseis de invertebrados marinhos. Depósitos marinhos estão intercalados com depósitos loessicos continentais da For-mação Libertad é recobertos pela Formação Dolores, com lamitos com concreções calcárias Os resultados da datação por radiocarbono mostraram idades calibradas de 13.900±200, 41.500±1.900 e 50.000±3.000 anos AP, para bioclastos de fósseis marinhos em uma elevação acima do nível médio do mar de -2,8, -6,13 e -8 m, respectivamente. Essas idades e alturas estão em desacordo com a evolução do nível do mar aceita para o final do Pleistoceno. Duas explicações possíveis são: (a) as idades representam as idades mínimas e a depositação ocorreu durante o último período interglacial (115-130 ka; MIS 5e), ou (b) representam as idades de depositação, mas a área experimentou grandes reajustes isostáticos durante e após a última glaciação. Várias linhas de evidência favorecem uma idade do Pleistoceno tardio para a Formação Chuy superior, incluindo a curva do 18O obtida para conchas de inverte-brados, que mostra grandes variações seculares consistentes com estágios MIS 1 a MIS 7. O cenário proposto prevê uma subsidência significativa entre 50-20 ka devido aos efeitos distantes da carga glacial nos Andes/Patagônia. Aos 20 ka, a regressão eustática superou a subsidência, levando à continentalização na área da La-guna de Rocha. As condições marinhas retornaram em 15 ka e no Holoceno, exceto para depósitos continentais (Forma-ção Dolores) em 11-10 ka (Younger Dryas). Uma subida de 115 m foi inferida entre 14 e 9 ka na área, o que é interpretado como um elevação pós-glacial. No Holoceno, uma subsidência moderada foi registrada novamente. Observa-se uma tendência regional, com um aumento dos depósitos marinhos em direção ao W-SW, o que é consistente com uma expli-cação como um rebote isostático pós-glacial.

De relatos y memorias: un análisis antropológico situado de las identidades de mujeres indígenas en una ciudad intermedia; Of stories and memories: a situated anthropological analysis of the identities of indigenous women in an intermediate city; De histórias e memórias: uma análise antropológica situada das identidades de mulheres indígenas em uma cidade intermediária Lencina, Rocio; Mariano, Mercedes El presente trabajo se ha constituido en un esfuerzo por comprender ciertos aspectos relativos a la producción social de discursos identitarios contemporáneos de mujeres que se adscriben étnicamente como indígenas e inscribirlos en el marco de un proceso social más amplio. La especificidad del tema propuesto se inscribe en la heterogeneidad de sentidos que dichas mujeres reconocen al definirse como indígenas, lo que permite comenzar a delinear un campo de investigación en un área donde este tema solo fue vinculado con el pasado a través de estudios históricos y arqueológicos. En este sentido, si bien existen estudios a nivel nacional, en el partido de Olavarría no se han llevado a cabo investigaciones de manera sistemática ni desde la perspectiva de sus protagonistas. Por ello, se propone construir conocimiento nuevo y actualizado a partir de un enfoque etnográfico que permita analizar y comprender los diversos procesos de construcción identitaria en una ciudad de tipo intermedia.; The present work has been constituted in an effort to understand certain aspects related to the social production of contemporary identity discourses of women who are ethnically ascribed as indigenous and to inscribe them within the framework of a broader social process. The specificity of the proposed theme is inscribed in the heterogeneity of meanings that these women recognize when defining themselves as indigenous, which allows beginning to outline a field of research in an area where this theme was only linked to the past through historical and archaeological studies. In this sense, although there are studies at the national level, in the city of Olavarría they have not been carried out systematically or from the perspective of their protagonists. For this reason, it is proposed to build new and updated knowledge through an ethnographic approach that allows analyzing and understanding the various processes of identity construction in an intermediate-type city.; O presente trabalho constituiu-se em um esforço para compreender alguns aspectos relacionados à produção social dos discursos identitários contemporâneos de mulheres etnicamente classificadas como indígenas e inscrevê-los no quadro de um processo social mais amplo. A especificidade do tema proposto se inscreve na heterogeneidade de significados que essas mulheres reconhecem ao se definirem como indígenas, o que permite começar a delinear um campo de pesquisa em uma área onde esse tema só estava ligado ao passado por meio de estudos históricos e arqueológicos. Nesse sentido, embora existam estudos em nível nacional, no partido de Olavarría eles não foram realizados de forma sistemática ou na perspectiva de seus protagonistas. Por esta razão, propõe-se construir novos e atualizados conhecimentos através de uma abordagem etnográfica que permita analisar e compreender os vários processos de construção identitária numa cidade de tipo intermédio.

A systematic review about the contribution of the genus Tessaria (Asteraceae) to cancer study and treatment; Revisión sistemática sobre la contribución del género Tessaria (Asteraceae) al estudio y tratamiento del cáncer; Revisão sistemática sobre a contribuição do gênero Vernonia condensada Baker (Asteraceae) para o estudo e tratamento do câncer Sosa Lochedino, Arianna Lourdes; Hapon, María Belén; Gamarra Luques, Carlos Diego Belonging to the Asteraceae family, Tessaria (Ruiz & Pavon, 1753) is a genus of shrubs or small trees distributedin various habitats located from Argentina to the southwestern United States of America. The taxa arecomposed of five confirmed species, which have been previously reported for their ethnopharmacologicaluses as hypocholesterolemiant, hypoglycemic, antitussive, anti-inflammatory, anticancer, and abortifacientagent; it also has been used in hepatic, renal, pulmonary, and rheumatic diseases. Up to the present, T.absinthioides, T. ambigua, T. dodoneifolia, T. fastigiata and T. integrifolia have been chemically analyzed.While decoctions, infusions, methanolic, and hydromethanolic extracts are the most studied botanicalcompounds, its most recognized phytochemical constituents are caffeoylquinic acid, eupatorin, naringenin,protocatechuic, and quercetin. Scientifically, several biomedical properties such as virucidal, antibacterial,leishmanicidal, insecticidal, gastroprotective, antiasthmatic, hypoglycemic, and antiatherogenic wereattributed to the genus. Concerning oncologic research, the chemicals produced by Tessaria have antitumoractivities interfering with carcinogenesis, cell proliferation, metastasis, and angiogenesis. In addition, thenatural extracts obtained from Tessaria species have biological activities closely related to cancer, actingas antioxidants and anti-inflammatories. In particular, it has been reported that T. absinthioides resultedcytotoxic against several cancer cell lines and acts as an antitumoral compound in murine models. Due tothe aforementioned antecedents, the Tessaria species are considered undervalued within the oncologicalfield; the goal of the current systematic review is to summarize the information available on the genus,relevant for cancer research and treatment.; Perteneciente a la familia Asteraceae, Tessaria (Ruiz & Pavon, 1753) es un género de arbustos o árboles pequeños distribuidos en diversos hábitats ubicados desde Argentina hasta el suroeste de los Estados Unidos de América. El taxón está compuesto por cinco especies confirmadas, las cuales han sido previamente reportadas por sus usos etnofarmacológicos como hipocolesterolemiante, hipoglucemiante, antitusivo, antiinflamatorio, anticanceroso y abortivo; así como en enfermedades hepáticas, renales, pulmonares y reumáticas. Hasta el momento, se han analizado químicamente T. absinthioides, T. ambigua, T. dodoneifolia, T. fastigiata y T. integrifolia. Mientras que las decocciones, infusiones, extractos metanólicos e hidrometanólicos son los compuestos botánicos más estudiados; sus constituyentes fitoquímicos más reconocidos son el ácido cafeolquínico, eupatorina, naringenina, protocatechuic y quercetina. Científicamente, se atribuyeron al género varias propiedades biomédicas como virucida, antibacteriano, leishmanicida, insecticida, gastroprotector, antiasmático, hipoglucemiante y antiaterogénico. En relación con la investigación oncológica, las sustancias químicas producidas por Tessaria, tienen actividades antitumorales que interfieren con la carcinogénesis, la proliferación celular, la metástasis y la angiogénesis. Además, los extractos naturales obtenidos de estas especies tienen actividades biológicas estrechamente relacionadas con el cáncer y actúan como antioxidantes y antiinflamatorios. En particular, se ha informado que T. absinthioides resulta citotóxico contra varias líneas de células cancerosas y actúa como compuesto antitumoral en modelos murinos. Debido a los antecedentes mencionados, se considera la especie Tessaria como infravalorada dentro del campo oncológico. El objetivo de la presente revisión sistemática es resumir la información disponible del género, relevante para la investigación y el tratamiento del cáncer.; Pertencente à família Asteraceae, a Vernonia (Ruiz & Pavon, 1753) é um gênero de arbustos ou pequenas árvores distribuídas em vários habitats localizados da Argentina ao sudoeste dos Estados Unidos da América. O táxon é composto por cinco espécies confirmadas, que foram previamente relatadas para seus usos etnofarmacológicos como hipocolesterolemiante, hipoglicêmico, antitussivo, anti-inflamatório, anticâncer e abortivo; assim como em doenças hepáticas, renais, pulmonares e reumáticas. Até agora, foram analisados quimicamente T. absinthioides, T. ambigua, T. dodoneifolia, T. fastigiata e T. integrifolia. Por outro lado, as decocções, infusões, extratos metanólicos e hidrometanólicos são os compostos botânicos mais estudados; seus constituintes fitoquímicos mais reconhecidos são: ácido cafeoilquínico, eupatorina, naringenina, protocatecuico e quercetina. Cientificamente, várias propriedades biomédicas foram atribuídas ao gênero como viricida, antibacteriana, leishmanicida, inseticida, gastroprotetor, antiasmática, hipoglicêmica e antiaterógena. Com relação à pesquisa sobre câncer, os produtos químicos produzidos pela Venonia têm atividades antitumorais que interferem na carcinogênese, proliferação celular, metástase e angiogênese. Além disso, extratos naturais obtidos dessas espécies têm atividades biológicas intimamente relacionadas com o câncer e atuam como antioxidantes e anti-inflamatórios. Em particular, t. absinthioides tem sido relatado como citotóxico contra várias linhas de células cancerosas e age como um composto antitumoral em modelos murinos. Devido ao passado supracitado, a espécie Vernonia é considerada desvalorizada dentro do campo oncológico. O objetivo desta revisão sistemática é resumir as informações do gênero disponíveis relevantes para a pesquisa e o tratamento do câncer.

Aproximación cuantitativa a la discriminación contra gays y lesbianas; Quantitative approach to discrimination against gays and lesbians Ortega, Julian En este artículo se exponen parte de los hallazgos de la investigación doctoral del autor, cuyo objetivo principal consistió en describir y analizar los procesos de discriminación, violencia y desigualdad contra trabajadores y trabajadoras gays y lesbianas en el sector de la salud del Área Metropolitana de Buenos Aires. La investigación adoptó un enfoque metodológico mixto. Se desarrollan parte de los hallazgos cuantitativos, más específicamente los resultados de la Escala de Discriminación Percibida y Escala de Victimización Percibida, para someterlos a discusión con otras investigaciones previas en las cuales fueron administradas. Se concluye que los hechos de discriminación y desigualdad hacia gays y lesbianas aún persisten en los espacios de trabajo de la salud, mientras que las manifestaciones más explicitas de violencia se habrían desplazado hacia la población travesti/trans.; This article derives from the author´s doctoral research, which analysed situations of discrimination, violence and inequality against gay and lesbian workers due to their sexual orientation, gender or gender expression in healthcare institutions located in the Metropolitan Area of Buenos Aires. The study was carried out using a mixed approach. Part of the analysis from quantitave methods is described, with emphasis on the results of the Perceived Discrimination Scale and the Perceived Victimization Scale in order to relate these data to previous findings from different research. It is possible to conclude that acts of discrimination and inequality toward gays and lesbians are still common within healthcare workplaces, but more explicit manifestations of violence might have displaced to the transgender community.

Formative Research to Design SMS Messages to Increase Breast Cancer Screening Uptake in Argentina Sánchez Antelo, Victoria Inés María; Paolino, Melisa Delia; Frejdkes, Paula; Correa, Silvina; Furia, Anabel; Lopez de Degani, Graciela; Arrossi, Silvina There is a need for interventions to promote breast cancer screening among high-risk population. This formative research is part of a pragmatic randomized trial being conducted in Santa Fe, Argentina, in which we are evaluating whether SMS messages sent to women aged 50-69 increases breast cancer screening uptake. Here, we describe the formative results used to design the content of the SMS messages to be tested in the trial. We conducted four on-line focus groups with women aged 50+ (n = 14). Participatory techniques were used to debate the advantages and disadvantages of different options for the five structural elements -i.e., greeting, recipient, sender, message’s topic, and closing line-of the SMS message. We openly coded the discussions for agreements and preferences regarding the SMS message content. SMS messages as reminders to increase breast cancer screening were highly accepted. Women argued that the greeting line should provide clear information about the topic of the SMS message (eg, Health information). SMS messages should also include the woman’s name, because this inclusion would be an indicator that it was a personal SMS message. Most women considered that the sender of the SMS messages should be a health institution as this would legitimize the content. Regarding the topic of the message, women preferred an imperative tone and they mentioned that the SMS message should include information about how to get a mammogram (eg, “Women aged 50-69 should have a mammogram done every two years. Do you have an appointment? WhatsApp to …”). The closing line should encourage women to get a mammogram (eg, “Ask for your appointment now! It is important!”). Our findings have some implications for the design of mHealth interventions targeted at improving breast cancer screening. A personalized SMS could be a good way of inviting women, although its content must be carefully designed to provide clear information about how to get a mammogram.

Presencias de lo digital en la narrativa chilena reciente: Matías Celedón, Cynthia Rimsky, Mike Wilson; Presences of the digital in the recent Chilean narrative: Matías Celedón, Cynthia Rimsky, Mike Wilson Walker Sanchez, Carlos El objetivo de este artículo es analizar cómo las herramientas digitales inciden en los procedimientos de escritura utilizados por tres novelas chilenas recientes: La filial (2012) de Matías Celedón, Leñador o ruinas continentales (2013) de Mike Wilson, La revolución a dedo (2020) de Cynthia Rimsky. Se trata de examinar cómo la puesta en primer plano del procedimiento determina una marcada orientación de estas escrituras hacia, por un lado, ciertas modalidades tradicionales de recuperación de estrategias de las vanguardias históricas, y por otro lado, cómo esa misma exhibición del procedimiento implica una fuerte marca de los problemas que enfrenta la escritura en la actualidad, en particular, en lo referido a una presión ejercida por lo digital. En este marco, se presentan reflexiones sobre los modos contemporáneos de narrar y los verosímiles literarios que se hacen posible en esta coyuntura.; The aim of this article is to analyze how digital tools impact the writing procedures used by three recent Chilean novels: La filial (2012) by Matías Celedón, Leñador o ruinas continentales (2013) by Mike Wilson, La revolución a dedo (2020) by Cynthia Rimsky. It is a question of examining how the foregrounding of the procedure determines a marked orientation of these writings towards, on the one hand, certain traditional modalities of recovering some strategies of the historical avant-gardes, and on the other hand, how that same exhibition of the procedure implies a strong mark of the problems that writing faces today, in particular, with regard to a pressure exerted by the digital world. Within this framework, we will proposes reflections on contemporary modes of narration and the literary verisimilitudes that are made possible at this context.

El arte rupestre de Cueva Huenul 1 (Neuquén, Argentina): un lugar persistente del noroeste de Patagonia; Rock art of Cueva Huenul 1 site (Neuquén, Argentina): a persistent place in northwestern Patagonia Romero Villanueva Badin, Guadalupe Se presentan los primeros resultados del análisis arqueológico del arte rupestre de Cueva Huenul 1 (CH1) (Neuquén, Patagonia, Argentina). El sitio posee una robusta secuencia cronoestratigráfic a que enmarca sus ocupaciones humanas entre los 12.000 y 300 años calibrados AP. Sus motivos se caracterizan a partir de diversas variables y se retoma información arqueométrica disponible sobre estas imágenes, destacando el novedoso aporte del fechado absoluto por AMS de algunas de ellas. Además, se postula una secuencia de producción de los motivos que es articulada con diversos indicadores para asignar una cronología a los momentos de ejecución definidos. Estos resultados son evaluados junto con otras evidencias registradas en el sitio que proveen información independiente pero complementaria sobre su historia de uso. Esta integración indica que la comunicación visual de información, materializada en el arte rupestre, desempeñó un rol fundamental ,aunque versátil, en la historia ocupacional del sitio y la región.Si bien CH1 fue ocupado en forma poco intensa y discontinua durante el Holoceno, su arte rupestre constituyó un factor marcatorio clave en la construcción humana del sitio y su entorno como ´lugar persistente´ dentro de la geografía social de los grupos del noroeste de Patagonia en el pasado.; This paper presents the initial results of the archaeological analysis of rock art from Huenul Cave 1 (CH1) (Neuquén, Patagonia, Argentina). The site’s robust chronostratigraphic sequence frames human occupations between 12,000-300 calibrated years BP. The paper classifies rock art motifs on the basis of several variables and the available archaeometric data, including recently obtained radiocarbon dating by AMS of some images. It also proposes a production sequence for the motifs by connecting temporal data from other proxies to assign a chronology to the different moments of rock art production. These results are assessed in conjunction with other evidence found at CH1, which provides independent but complementary data regarding the history of its use. This integration indicates that visual communication of information, which took the form of rock art, played a fundamental –yet versatile– role in the occupational history of the site and the region. While the occupation of CH1 was neither intense nor continuous during the Holocene, rock art was a key placemaking device that shaped CH1 as a “persistent place” in the social geography of human groups in northern Patagonia.

La otra cara de la transformación vitivinícola: los pequeños productores mendocinos; The other side of wine processing: small producers in Mendoza Cerdá, Juan Manuel El sector vitivinícola de la provincia de Mendoza estuvo asociado históricamente al trabajo de los inmigrantes europeos que llegaron a la región a finales del siglo XIX y comienzos del XX y que accedieron a pequeñas parcelas de tierra donde comenzaron a cultivar la vid. Esta asociación de la pequeña propiedad con el cultivo de la vid se ha visto reforzada aún más en comparación a las grandes extensiones de tierras de la pampa húmeda, lo que ha dado un sentido de identidad particular. Sin embargo, en las últimas décadas, esto a comenzado a cambiar a partir del proceso de reconversión vitivinícola, que tuvo como una de sus características la concentración de la tierra y la pérdida de los pequeños productores. Sin embargo, este segmento sigue siendo una parte central de la vitivinicultura mendocina. Este artículo analiza en detalle este proceso, los impactos sobre la configuración del espacio y las políticas públicas que han contribuido al sostenimiento de los pequeños viñateros en un contexto de fuertes transformaciones en el sector.; The wine sector of the province of Mendoza was historically associated with the work of European immigrants who arrived in the region in the late nineteenth and early twentieth centuries and who accessed small plots of land where they began to cultivate the vine. This association of small property with vine cultivation has been further forced compared to the large tracts of land in the humid pampas, which has given a particular sense of identity. However, in recent decades, this has begun to change from the process of wine conversion that had a sone of its characteristics the concentration of land and the loss of small producers. However, this segment remains a central part ofMendoza viticulture. This article analyzes in detail this process, the impacts on the configuration of space and the public policies that have contributed to the sustenance of small winemakers in a context of strong transformations in the sector.