Modelos multivariados en la búsqueda de regiones genómicas para resistencia a Mal de Río Cuarto y bacteriosis en maiz; Multi traits models for genomic regions associated with Mal de Río Cuarto and bacterial disease in maize Ruiz, Marcos; Rossi, Ezequiel Alejandro; Bonamico, Natalia Cecilia; Balzarini, Monica Graciela La producción de maíz (Zea Mays L.) ha sido ampliamente beneficiada con la mejora de líneas endocriadas respecto a la resistencia a enfermedades causadas por virus y hongos.Sin embargo, es notable la ausencia de genotipos resistentes a bacteriosis. El objetivo delpresente estudio fue identificar regiones genómicas para la mejora de resistencia a Mal deRío Cuarto (MRC) y a bacteriosis (BD) en un germoplasma diverso de maíz. Se evaluó, paraambas enfermedades, una población diversa de líneas de maíz en el ciclo de cultivo 2019-2020en la región argentina donde la virosis MRC es endémica. Se estimó incidencia y severidad deMRC y BD en cada línea y se realizó un estudio de mapeo por asociación (GWAS) con 78.376marcadores SNPs. Un modelo multicarácter se utilizó para evaluar simultáneamente laresistencia a MRC y BD en las líneas evaluadas. El germoplasma evidenció alta variabilidadgenética tanto para la mejora de la resistencia a MRC como a BD, pero no se observócorrelación genética significativa entre la respuesta a ambas enfermedades. Se identificaronregiones genómicas promisorias para resistencia a MRC y a BD, que serán confirmadas enevaluaciones en nuevos ambientes.; Maize (Zea Mays L.) production has been greatly benefited from the improvement of inbred lines in regard to the resistance to diseases. However, the absence of resistant genotypes to bacteriosis is remarkable. The aim of the study was to identify genomic regions for resistance to Mal de Río Cuarto (MRC) and to bacterial disease (BD) in a diverse maize germplasm evaluated in the Argentinian region where MRC virus is endemic. A maize diverse population was assessed for both diseases during the 2019-2020 crop season. Incidence and severity of MRC and BD were estimated for each line and a genome wide association study (GWAS) was conducted with 78,376 SNP markers. A multi-trait mixed linear model was used for simultaneous evaluation of resistance to MRC and BD in the scored lines. The germplasm showed high genetic variability for both MRC and BD resistance. No significant genetic correlation was observed between the response to both diseases. Promising genomic regions for resistance to MRC and BD were identified and will be confirmed in further trials.

Inhibition of flippase-like activity by tubulin regulates phosphatidylserine exposure in erythrocytes from hypertensive and diabetic patients Muhlberger, Tamara; Balach, Melisa Micaela; Bisig, Carlos Gaston; Santander, Verónica Silvina; Monesterolo, Noelia Edith; Casale, Cesar Horacio; Campetelli, Alexis Nazareno Plasma membrane tubulin is an endogenous regulator of P-ATPases and the unusual accumulation of tubulin in the erythrocyte membrane results in a partial inhibition of some their activities, causing hemorheological disorders like reduced cell deformability and osmotic resistance. These disorders are of particular interest in hypertension and diabetes, where the abnormal increase in membrane tubulin may be related to the disease development. Phosphatidylserine (PS) is more exposed on the membrane of diabetic erythrocytes than in healthy cells. In most cells, PS is transported from the exoplasmic to the cytoplasmic leaflet of the membrane by lipid flippases. Here, we report that PS is more exposed in erythrocytes from both hypertensive and diabetic patients than in healthy erythrocytes, which could be attributed to the inhibition of flippase activity by tubulin. This is supported by: (i) the translocation rate of a fluorescent PS analog in hypertensive and diabetic erythrocytes was slower than in healthy cells, (ii) the pharmacological variation of membrane tubulin in erythrocytes and K562 cells was linked to changes in PS translocation and (iii) the P-ATPase-dependent PS translocation in inside-out vesicles (IOVs) from human erythrocytes was inhibited by tubulin. These results suggest that tubulin regulates flippase activity and hence, the membrane phospholipid asymmetry.

Association mapping to identify molecular markers associated with resistance genes to stink bugs in soybean Ghione, Celina Elena; Lombardo, Lucio Andrés; Vicentin, Ignacio Gabriel; Heinz, Ruth Amelia Damage generated by insects is one of main restricting factors for soybean production. Stink bugs are a great threat within pests because, by feeding mainly on pods, they cause direct and irreversible damage to developing seeds. Thus, plant resistance is an important management strategy to reduce insect population impact on yield losses. Association mapping can be used as a powerful tool for dissecting resistance mechanisms in soybean, more specifically to recover functional loci involved in plant defense against herbivorous insects; and can also provide valuable markers for the development of soybean cultivars with resistance. The purpose of this study was to identify molecular markers associated with resistance genes to stink bugs in a collection of soybean germplasm, using the association mapping strategy. According to the decline value in the linkage disequilibrium, an accurate power of mapping resolution was predicted in this population. Four associated markers located in chromosomes 6 and 15 were identified. Out of the 112 candidate genes close to them, 31 would encode proteins related to defense pathways triggered by the attack of herbivorous insects. The proteins encoded by these candidate genes could be associated to the jasmonic acid pathway. The main contribution of this study was the identification of molecular markers associated with resistance genes to stink bugs. These markers will be a useful tool for marker-assisted selection applied to soybean genetic breeding.

The condition number of the static gains matrix as a quality index in LPV IO MIMO multi-objective identification Chuk, Oscar Daniel; Scaglia, Gustavo Juan Eduardo; Rodriguez Medina, Carlos Gustavo A quality index of linear multivariable models is presented in this paper, with application to linear parameter varying (LPV) systems. The index is based on the condition number of the static gains matrix of the process. An example of use in the identification by means of multi-objective optimisation of a non-linear process of two inputs and two outputs verifies the importance of the use of such index, in particular, if the identified model will be used for the synthesis of controllers.

Aspect ratio of nano/microstructures determines Staphylococcus aureus adhesion on PET and titanium surfaces Meinshausen, A. K.; Herbster, M.; Zwahr, C.; Soldera, Marcos Maximiliano; Müller, A.; Halle, T.; Lasagni, A. F.; Bertrand, J. Aims: Joint infections cause premature implant failure. The avoidance of bacterial colonization of implant materials by modification of the material surface is therefore the focus of current research. In this in vitro study the complex interaction of periodic structures on PET and titanium surfaces on the adhesion of Staphylococcus aureus is analysed. Methods and Results: Using direct laser interference patterning as well as roll-to-roll hot embossing methods, structured periodic textures of different spatial distance were produced on surfaces and S. aureus were cultured for 24 h on these. The amount of adhering bacteria was quantified using fluorescence microscopy and the local adhesion behaviour was investigated using scanning electron microscopy. For PET structures, minimal bacterial adhesion was identified for an aspect ratio of about 0·02. On titanium structures, S. aureus adhesion was significantly decreased for profile heights of < 200 nm. Our results show a significantly decreased bacterial adhesion for structures with an aspect ratio range of 0·02 to 0·05. Conclusions: We show that structuring on surfaces can decrease the amount of S. aureus on titanium and PET as common implant materials. Significance and Impact of the Study: The study highlights the immense potential of applying specific structures to implant materials to prevent implant colonization with pathogen bacteria.

Versatile nanoadsorbents based on magnetic mesostructured silica nanoparticles with tailored surface properties for organic pollutants removal Peralta, Marcos Emanuel; Martire, Daniel Osvaldo; Moreno, Mario Sergio Jesus; Parolo, María Paula; Carlos, Luciano This paper addresses the development of new magnetic silica-based nanoadsorbents and evaluates their potential application in the removal of contaminants of emerging concern (CECs), polyaromatic hydrocarbons (PAHs) and aliphatic hydrocarbons. For this purpose, magnetic iron oxide nanoparticles were covered with a hybrid shell of silica and 3-(trimethoxysilyl)propyl-octadecyldimethyl-Ammonium chloride (TPODAC) as structure directing agent. The as-prepared hybrid material (MMST) was further modified with trimethoxyphenylsilane, obtaining a phenyl-functionalized nanoadsorbent (MMST-Ph). Both materials were thoroughly characterized with diverse physicochemical techniques, and batch sorption tests with single-contaminant and with mixtures of contaminants were performed. MMST-Ph proved to be more efficient for the adsorption of PAHs and aliphatic hydrocarbons. The presence of TPODAC and phenyl moieties anchored on the mesostructured silica frameworks resulted to be a key factor to obtain high PAHs uptakes from aqueous media. In the case of CECs, ibuprofen (IBU), diclofenac (DCF) and carbamazepine (CBZ) were tested. These experiments demonstrated that even though MMST possesses better adsorption capacities of CECs, MMST-Ph achieved high IBU and DCF uptakes. Regeneration and reuse experiments showed that MMST-Ph can be reused in eight cycles without losing the adsorption capacity of anthracene. In the case of MMST, there was a 42% drop in the adsorption capacity of ibuprofen in the second cycle, whereas in the next seven cycles the adsorption capacity remained constant.The promising results obtained in this work strengthen the potential application of surface-modified magnetic silica nanoparticles for the removal of different types of organic pollutants from waters.

Influence of plastic deformation in fatigue crack behavior in bainitic steel Marinelli, María Cecilia; Balbi, Marcela Ángela; Krupp, U. In the present paper, the behavior of fatigue microcracks is studied in a high-strength bainitic steel with the aim of identifying the microstructural features that influence the mode of initiation and propagation of microcracks. For this study, a gradual monitoring of surface damage during low-cycle fatigue test at different plastic strain ranges was carried out by means of light optical and electron microscopy along with EBSD data analysis. Moreover, the dislocation structure near the surface was analyzed and correlated with the crack initiation site. The results showed a change with respect to cracking mechanisms from 0.2% plastic strain.

The politics of waste picking: Reflections from the upscaling of a co-management model for recyclable waste in Buenos Aires (Argentina) Carenzo, Sebastian; Sorroche, Santiago In Argentina, the organization of waste picker cooperatives has enabled these organizations to impose their approach and point of view in the system of waste management derived from the Integrated Solid Waste Management (ISWM) model. In this context, the experience of the cooperatives of the Autonomous City of Buenos Aires (CABA) stands out, as it paved the way for a co-management model that was unprecedented in the country. Since then, the "Buenos Aires System" has been promoted by the Argentine federation of waste pickers (FACCyR), as the model to be implemented throughout the country. The differences between the CABA and other jurisdictions raises significant questions regarding the adaptability of this model. Based on a Political Ecology of Waste, we address this question by analyzing the process of expansion of this model following the Jóvenes en Progreso cooperative in the municipality of Lomas de Zamora (Province of Buenos Aires).; En Argentine, l'organisation des coopératives de récupérateurs de déchets a été capable d’imposer dans le régime de gestion des déchets en place (modèle de gestion intégrée des déchets solides-GIDS) promu par des agences d'État et les organisations multilatérales, l’intégration de leurs approche, voix et perspectives. A ce titre, l'expérience des coopératives de récupération de déchets de la ville autonome de Buenos Aires retient notre attention pour introduire un modèle de gestion sociale des matières recyclables. Ce que l’on peut appeler le « système de Buenos Aires » a été promu par la fédération argentine des récupérateurs de déchets (Federación Argentina de Cartoneros, Carreros y Recicladores - FACCyR), en vue de son introduction dans les autres municipalités du pays. La ville de Buenos Aires affiche cependant de grandes inégalités sur le plan démographique, budgétaires et des infrastructures par rapport aux autres juridictions. Ces différences soulèvent des questions importantes quant aux possibilités d’adaptation dans d'autres municipalités. Sur la base d'une écologie politique des déchets, nous abordons cette question en analysant le processus de mise en œuvre de ce modèle dans la commune de Lomas de Zamora (Province de Buenos Aires) qui, malgré des frontières partagées avec la ville de Buenos Aires, présente des indicateurs sociodémographiques et budgétaires très contrastés. En suivant la trajectoire de la coopérative Jóvenes en Progreso, nous montrons comment le « système de Buenos Aires » constitue un cadre à la fois symbolique et politique pour ancrer leurs demandes un tant que secteur unifié tout en adaptant le modèle au contexte local. L’analyse de ce cas contribue à rendre compte des processus de politisation et territorialisation de la récupération des déchets, en mettant en évidence une pratique éloignée de l’image misérabiliste la réduisant à une activité de survie.

Kinetically stable nonequilibrium gold‐cobalt alloy nanoparticles with magnetic and plasmonic properties obtained by laser ablation in liquid Guadagnini, Andrea; Agnoli, Stefano; Badocco, Denis; Pastore, Paolo; Pilot, Roberto; Ravelle Chapuis, Régis; Fernández van Raap, Marcela Beatriz; Amendola, Vincenzo Nonequilibrium nanoalloys are metastable solids obtained at the nanoscale under nonequilibrium conditions that allow the study of kinetically frozen atoms and the discovery of new physical and chemical properties. However, the stabilization of metastable phases in the nanometric size regime is challenging and the synthetic route should be easy and sustainable, for the nonequilibrium nanoalloys to be practically available. Here we report on the one-step laser ablation synthesis in solution (LASiS) of nonequilibrium Au−Co alloy nanoparticles (NPs) and their characterization on ensembles and at the single nanoparticle level. The NPs are obtained as a polycrystalline solid solution stable in air and water, although surface cobalt atoms undergo oxidation to Co(II). Since gold is a renowned plasmonic material and metallic cobalt is ferromagnetic at room temperature, these properties are both found in the NPs. Besides, surface conjugation with thiolated molecules is possible and it was exploited to obtain colloidally stable solutions in water. Taking advantage of these features, an array of magnetic-plasmonic dots was obtained and used for surface-enhanced Raman scattering experiments. Overall, this study confirms that LASiS is an effective method for the formation of kinetically stable nonequilibrium nanoalloys and shows that Au−Co alloy NPs are appealing magnetically responsive plasmonic building blocks for several nanotechnological applications.

Irradiation dose does not affect male reproductive organ size, sperm storage, and female remating propensity in Ceratitis capitata Abraham, Solana; Diaz, Viviana Alejandra; Moyano, Andrea del Huerto; Castillo, Gisela Mariana; Rull Gabayet, Juan Antonio; Suárez, Lorena; Murua, Fernado; Pantano, Valeria; Molina, Diego; Ovruski Alderete, Sergio Marcelo The Mediterranean fruit fly Ceratitis capitata is a globally invasive pest, often controlled with the sterile insect technique (SIT). For the SIT, mass-rearing of the target insect followed by irradiation are imperatives. Sterile males are often less able to inhibit female remating and transfer less number of sperm, and even irradiation could affect male reproductive organs, with consequences for their ability to inhibit female remating. On the other hand, male age could affect their ability to modulate female response after mating. Here, we evaluated the quality of the genetic sexing strain Vienna-8-tsl mass-reared in Bioplanta San Juan, Argentina, under laboratory conditions, with regard to: (i) the ability of sterile males irradiated at 100 or 140 Gy to inhibit female remating, in the same day and at 24 h of first copulation; (ii) the ability of 3, 4 or 5 day-old sterile males to inhibit female remating at 24 h of first copulation, and (iii) the effect of a reduction in irradiation doses on the number of sperm stored by females and reproductive organ size in virgin males. Sterile males were better able than wild males to inhibit female remating in the same day of first copulation and as able as wild males 1 day after first copulation. Male age did not affect their ability to inhibit female receptivity. Number of sperm stored by females, testes size and ectodermal accessory glands size were not affected by male identity, while sterile 100 Gy males had larger mesodermal accessory glands than control lab males. A reduction in irradiation dose does not impact any variable measured, except for percentage of sperm-depleted females: Females mated with sterile 100 Gy males had lower probabilities to store sperm. The results showed here are very encouraging for tsl Vienna 8 strain reared in Argentina and are discussed in comparison with previous studies in C. capitata female remating with dissimilar results.

High-linearity front-end circuit for remote grounded capacitive sensors Haberman, Marcelo Alejandro; Spinelli, Enrique Mario; Reverter, Ferran This article presents a novel front-end circuit for remote grounded capacitive sensors. The circuit is insensitive to amplifier input capacitance and stray interconnecting-cable capacitances. It does not rely on neutralization or compensation techniques that require manual adjustments and high-accuracy reference components. The proposed solution reduces the circuit input capacitance below a few femptofarads using a simple but properly shielded custom transformer. In addition, the circuit keeps a nonlinearity error below 0.01% when measuring capacitances of units or tens of picofarad, even with up to 12-m interconnecting cables.

Los efectos de los ilegalismos sobre la materialidad de la escuela: El caso de una escuela emplazada en un contexto de pobreza urbana del conurbano bonaerense; The effects of illegalisms on the materiality of the school: The case of a school located in a context of urban poverty in the Buenos Aires suburbs Bonilla Muñoz, Marco Antonio El presente artículo tiene como objetivo analizar los efectos que tienen los ilegalismos sobre la materialidad de una escuela emplazada en un contexto de pobreza urbana del conurbano bonaerense. Para lo cual se presentan una serie de escenas de la vida escolar reconstruidas a través del trabajo de campo realizado durante los años 2016-2019. A modo de hipótesis sostenemos que estos efectos exacerban la fragilidad de las condiciones materiales de la escuela, y repercuten sobre la cotidianidad escolar de estudiantes, directivos y docentes. Resaltamos la importancia de pensar la materialidad de las escuelas como uno de los rasgos en los que hoy se expresa la desigualdad educativa.; The objective of this article is to analyze the effects that illegalisms have on the materiality of a school located in a context of urban poverty in the Buenos Aires suburbs. For this, a series of scenes from school life constructed through fieldwork carried out during the years 2016 - 2019 are presented. By way of hypothesis, we maintain that these effects exacerbate the fragility of the material conditions of the school and have an impact on the daily school life of students, managers and teachers. We highlight the importance of thinking about the materiality of schools as one of the features in which educational inequality is expressed today

La literatura brasileña de autoría negra y sus posicionamientos frente a las operaciones blancocéntricas del canon hegemónico; Brazilian literature of black authorship and its positions with white-centric operations of the hegemonic canon Tennina, Lucía En este artículo, en primer lugar, se presenta una lectura del perfil que define a la historia de la literatura brasileña, dando cuenta de su blancocentrismo, con pocas excepciones. También, se lleva a cabo un análisis de las bases históricas y conceptuales que fueron forjando un circuito y una escena literarios de autoría negra. Al mismo tiempo, se analizan las operaciones sobre el archivo de la esclavitud que realizan los escritores de autoría negra en diálogo con el canon hegemónico. El corpus de poemas que componen este artículo se concentra en autores del siglo XX: Carolina Maria de Jesus, Edimilson de Almeida Pereira, Eliane Marques, Oswaldo de Camargo, Cuti, Conceição Evaristo, Carlos Assumpção, Mel Duarte. El artículo parte de una concepción ampliada de literatura, por lo que, junto con la literatura de autoría negra, se hace mención también a prácticas culturales no literarias (como la fotografía y las artes plásticas).; In this article, first of all, a reading of the profile that defines the history of Brazilian literature is presented, accounting for its white-centeredness, with few exceptions. Also, is carried out an analysis of the historical and conceptual bases that were forging a circuit and a literary scene of black authorship. At the same time, the operations on the archive of slavery carried out by writers of black authorship in dialogue with the hegemonic canon are analyzed. The corpus of poems that make up this article focuses on authors of the 20th century: Carolina Maria de Jesus, Edimilson de Almeida Pereira, Eliane Marques, Oswaldo de Camargo, Cuti, Conceição Evaristo, Carlos Assumpção, Mel Duarte. The article starts from an expanded conception of literature, so that along with black authorship literature mention will also be made of non-literary cultural practices (such as photography and plastic arts).

Quercetin alleviates acute kidney injury by inhibiting ferroptosis Wang, Yuemin; Quan, Fei; Cao, Qiuhua; Lin, Yanting; Yue, Chongxiu; Bi, Ran; Cui, Xinmeng; Yang, Hongbao; Yang, Yong; Birnbaumer, Lutz; Li, Xianjing; Gao, Xinghua Introduction: Ferroptosis is an iron-dependent regulated necrosis and has been proven to contribute to the progress of acute kidney injury (AKI). Quercetin (QCT), a natural flavonoid which is commonly found in numerous fruits and vegetables, has extensive pharmacological effects, such as anti-oxidant, anti-inflammatory and anti-senescence effects. Objectives: This study aims to explain whether ferroptosis is a therapeutic strategy to AKI, and to explore the effect of QCT on AKI ferroptosis. Methods: NRK-52E cells and HK-2 cells were used for in vitro ferroptosis studies. Morphology of cells was detected by transmission electron microscopy. Lipid ROS was assayed using flow cytometry. In vivo, AKI was induced by ischemia–reperfusion (I/R) or folic acid (FA). To explore the molecular mechanisms, RNA-sequence analysis was performed. Transwell was used to detect macrophage migration. Results: We discovered that quercetin (QCT), a natural flavonoid, inhibited ferroptosis in renal proximal tubular epithelial cells. QCT blocked the typical morphologic changes of ferroptotic cells by reducing the levels of malondialdehyde (MDA) and lipid ROS and increasing the levels of glutathione (GSH). Moreover, QCT ameliorated AKI induced by I/R or FA. RNA-sequence analysis highlighted activation transcription factor 3 (ATF3), as it was the dominant one among all the 299 down-regulated genes by QCT. Knockdown of ATF3 could significantly increase the levels of SLC7A11, GPX4 and increased the cell viability. In addition, ferroptotic cells were found to be extremely pro-inflammatory by recruiting macrophages through CCL2, while QCT inhibited the chemotaxis of macrophages induced by ferroptosis in AKI. Conclusions: Collectively, these results identify QCT as a ferroptosis inhibitor and provide new therapeutic strategies for diseases related to ferroptosis.

Biopesticidal silo bag prepared by co-extrusion process Herrera, Jimena María; Zygadlo, Julio Alberto; Strumia, Miriam Cristina; Peralta, Elisabeth Silo bag technology is used for storing grains in a hermetic plastic structure. The major limitation of this structure is resistance to improper handling and external aggression, thus promoting pest incidence due to alterations in its internal atmosphere. In this study, we developed a biopesticidal silo bag consisting of a co-extruded three-layer film made of polyethylene and essential oil of Mentha piperita (7% w/w) in the inner layer. A film with the same structure but without essential oil was produced to be used as a control. The presence of the biopesticide in the silo bag was confirmed by FTIR-spectroscopy. Thus, mechanical, optical and chemical properties were evaluated. Diffusion coefficient of biopesticide from silo bag at 15, 25 and 35 °C was estimated as 6.4 ± 0.1 × 10-11, 1.45 ± 0.17 × 10-10 and 1.30 ± 0.2 × 10-10 cm2/s, respectively. Finally, the biopesticide silo bag was tested against Rhyzopertha dominica, primary pest of stored grain, showing 100 % of mortality during the time assayed (7 days). Hence, the incorporation of biopesticide by co-extrusion technology (low-cost and efficient machinery) into the inner layer of a silo bag could help to replace synthetic pesticides and avoid manipulation of these in the field, preventing biotic infestation.

Examining Neanderthal and carnivore occupations of Teixoneres Cave (Moià, Barcelona, Spain) using archaeostratigraphic and intra-site spatial analysis Zilio, Leandro; Hammond, Heidi; Karampaglidis, Theodoros; Sánchez Romero, Laura; Blasco, Ruth; Rivals, Florent; Rufà, Anna; Picin, Andrea; Gema Chacón, M.; Demuro, Martina; Arnold, Lee J.; Rosell, Jordi Teixoneres Cave (Moià, Barcelona, Spain) is a reference site for Middle Palaeolithic studies of the Iberian Peninsula. The cave preserves an extensive stratigraphic sequence made up of eight units, which is presented in depth in this work. The main goal of this study is to undertake an initial spatial examination of Unit III, formed during Marine Isotope Stage 3, with the aim of understanding spatial organization and past activities developed by Neanderthals and carnivores (bears, hyenas and smaller carnivores). The total sample analysed includes 38,244 archaeological items and 5888 limestone blocks. The application of GIS tools allows us to clearly distinguish three geologically-defined stratigraphic subunits. Unit III has been previously interpreted as a palimpsest resulting from alternating occupation of the cave by human groups and carnivores. The distribution study shows that faunal specimens, lithic artefacts, hearths and charcoal fragments are significantly concentrated at the entrance of the cave where, it is inferred, hominins carried out different activities, while carnivores preferred the sheltered zones in the inner areas of the cave. The results obtained reveal a spatial pattern characterized by fire use related zones, and show that the site was occupied by Neanderthals in a similar and consistent way throughout the ˃ 7000 years range covered by the analysed subunits. This spatial pattern is interpreted as resulting from repeated short-term human occupations.

En busca del nicho apropiado: Lógicas de acción de las escuelas y el surgimiento de un escenario institucional diversificado; Finding the right niche. Schools logics of action and the emergence of diversified institutional landscape Gottau, Verónica; Mayer, Liliana Judith Este trabajo parte de la hipótesis de que las escuelas primarias y los colegios secundarios despliegan diferentes estrategias para determinar su perfil y proyecto institucional para definir los públicos que desean albergar. A partir del marco conceptual de las lógicas de acción propuesto por Ball y Maroy (2009) y Van Zanten (2009), realizamos un análisis de las lógicas de acción que implementan las 14 escuelas no subvencionadas de la Ciudad de Buenos Aires ubicadas en la Comuna 13 para determinar su posicionamiento dentro del espectro educativo. Entre los principales resultados, destacamos cuatro tipos de lógicas de acción predominantes, aunque no excluyentes, que tienden a determinar el perfil de los alumnos de la escuela y su posicionamiento social: (a) lógicas customizadas —escuelas a la carta—; (b) lógicas de ensamblaje —escuelas de largo plazo—; (c) lógicas vanguardistas —escuelas de punta—, y (d) lógicas globales —escuelas que se internacionalizan. Concluimos que las escuelas no son actores pasivos o meros receptores de las elecciones de los padres. A través de sus lógicas de acción y estrategias de posicionamiento, intentan proyectar una imagen en línea con el proyecto institucional y el perfil de los alumnos o, más precisamente, el perfil de los padres que desean capturar. El análisis hasta aquí expuesto permite comprender los procesos de diversificación de instituciones dentro de circuitos homologables entre sí.; This work relies on the hypothesis that primary and secondary schools develop strategies to determine the social position of the school in the local area and the profile of the enrolment they try to capture. We draw in the conceptual framework proposed by Ball y Maroy (2009) y Van Zanten (2009) to analyse the logics of action implemented by 14 non-subsidized schools located in the borough ‘Comuna 13’ in the City of Buenos Aires. The results show that there are four mainstream though not exclusive logics of action: (a) Customized logics of actions: school a la carte; (b) Assembly logics: in the long run schools; (c) Avant-garde logics: cutting edge schools and (d) Worldwide Logics: schools that go global. We conclude that schools are not passive receivers of students or parents’ schools choice. They develop strategies and implement different logics of action to position themselves in the local area and determine school enrolment, or more specifically, the profile of the families they aim at. This work sheds some light on the processes of diversification of schools with similar characteristics located in the same neighbourhood.

Síndrome de Cushing en un paciente canino con carcinoma cortical adrenal: Reporte de un caso; Cushing syndrome in a canine patient with adrenal cortical carcinoma: A case report Maurenzig, Nelson Damian; Cainzos, Romina Paola El objetivo de este trabajo fue resaltar la importancia del diagnóstico de la causa del síndrome de Cushing, en un paciente canino macho, de 12 años de edad, con carcinoma cortical adrenal, que llega a la consulta por presentar signos clínicos compatibles con Síndrome de Cushing. Los resultados de la estimulación con ACTH sintética (Synacthen 0.25 mg®) arrojaron un aumento en el cortisol basal (10.8 µg/dl) y pos-estimulación (28.4µg/dl). La ecografía abdominal reveló la glándula adrenal izquierda de forma redondeada y aumentado de tamaño (3.3 x 3.4 cm), compatible con neoplasia adrenal. El tratamiento de elección fue la adrenalectomía de la glándula adrenal izquierda por abordaje abdominal en la línea media y posterior envío a histopatología para su evaluación, el cual confirmó un carcinoma cortical adrenal. La adrenalectomía en estos casos aumenta la supervivencia, mejorando así la calidad de vida del paciente.; The aim of this work was to highlight the importance of diagnosing the cause of Cushing's syndrome in a 12-year-old male canine patient with adrenal cortical carcinoma, who came to the clinic for presenting clinical signs compatible with Cushing's syndrome. The results of stimulation with synthetic ACTH (Synacthen 0.25 mg®) showed an increase in basal cortisol (10.8 μg/dl) and post-stimulation (28.4 μg/dl). Abdominal ultrasound revealed a rounded and enlarged left adrenal gland (3.3 x 3.4 cm), compatible with adrenal neoplasia. The treatment of choice was adrenalectomy of the left adrenal gland due to a midline abdominal approach and subsequent referral to histopathology for evaluation, which confirmed adrenal cortical carcinoma. Adrenalectomy in these cases increases survival, thus improving the quality of life of the patient.

Bifunctional GM-CSF-derived peptides as tools for O-glycoengineering and protein tagging Sales, María de Los Milagros; Kratje, Ricardo Bertoldo; Oggero Eberhardt, Marcos Rafael; Ceaglio, Natalia Analia Rapid development of effective biotherapeutics has been a concern during the last couple decades. In our work we designed two novel peptide tags, GMOP and mGMOP, derived from the N-terminal region of human granulocyte and macrophage colony stimulating factor (hGM-CSF), which contain four and six potential O-glycosylation sites, respectively. These peptide tags were fused to the N-terminus of human interferon-α2b (hIFN-α2b), a therapeutic antiviral and antiproliferative protein rapidly cleared from circulation. Two new molecules were obtained which, consistently with the presence of O-glycans, showed higher molecular masses, more negatively charged isoforms, and higher sialic acid content compared to wild-type IFN. In vitro bioactivity of purified chimeras revealed a similar antiviral specific biological activity (SBA) compared to unmodified IFN. A reduction of antiproliferative SBA was only observed for mGMOP-IFN. Pharmacokinetic studies in rats showed a notable improvement in terminal half-life (t1/2elim) (3.3 and 2.8 times-longer) and a marked reduction of the apparent clearance (CLapp, 3.7 and 4.1-fold lower for GMOP-IFN and mGMOP-IFN in comparison with native IFN, respectively). Furthermore, the in vitro thermal and plasma stability of both proteins was improved. Finally, a monoclonal antibody (mAb) that recognizes an N-terminal GM-CSF epitope was able to bind both chimeras in western blots and ELISAs. This demonstrates the potential of both peptides to behave as bifunctional tags to create novel long-acting biotherapeutics and to facilitate detection and purification.

La irrupción del tractor en los caminos de la pampa bonaerense a finales de la década de 1950 y principios de la de 1960: significados, usos y conflictos; The Irruption of the Tractor on the Roads of the Buenos Aires Pampas in the Late Fifties and Early Sixties: Meanings, Uses and Conflicts; O advento do trator nos caminhos da pampa bonaerense no final dos anos 1950 e início dos anos de 1960: significados, usos e conflitos Salomón, Alejandra Laura Dentro de los recientes estudios rurales la movilidad ha cobrado creciente interés académico, pero la historia de la misma mantiene enormes vacíos, a pesar de encontrase atravesada por significados, experiencias y conflictos que trascienden el tiempo presente. Frente a tal vacancia, la propuesta del artículo consiste en examinar las implicancias socio-políticas de la irrupción del tractor en la pampa bonaerense a fines de los años cincuenta y principios de los sesenta, considerando al mismo menos como elemento de trabajo y más como instrumento de transporte. En particular, se incursionará, por un lado, en sus valores ambivalentes (progreso versus destrucción) y su utilización como vehículo no convencional de desplazamiento de personas y productos. Y, por otro, en la acción estatal punitiva y educativa frente al deterioro de caminos rurales que tales prácticas de movilidad generaron. Por medio de un corpus diverso de fuentes primarias textuales y visuales se demostrará que la mecanización ocurrida en el agro bonaerense trascendió el ámbito meramente productivo, motivando perjuicios viales y desafiando el ordenamiento normativo de tránsito.; Within recent rural studies, mobility has gained increasing academic interest, but its history maintains enormous gaps, despite being crossed by meanings, experiences and conflicts that go beyond the present time. Faced with such a void, the aim of the article is to examine the socio-political implications of the diffusion of the tractor in the Buenos Aires Pampa region in the late fifties and early sixties, considering it less as a work item and more as an instrument of transportation. Specifically, it will explore, firstly, its ambivalent values —progress in contrast to destruction— and its use as an unconventional vehicle for the transportation of people and products. Secondly, it will research the state action –punitive and educational– against the deterioration of rural roads that such mobility practices generated. By means of a diverse corpus of primary textual and visual sources, it will be shown that the mechanization that occurred in the agricultural area of Buenos Aires transcended the merely productive sphere, motivating road damage and challenging the regulatory ordering of traffic.; Dentro dos recentes estudos rurais, a mobilidade tem tido um crescente interesse acadêmico, mas a história sobre a mesma mantém enormes vazios, apesar de se encontrar atravessada por significados, experiências e conflitos que transcendem o tempo presente. Frente a tal ausência, a proposta do artigo consiste em examinar as implicações sóciopolíticas do advento do trator na pampa bonaerense, no final dos anos cinquenta e início dos anos sessenta, o considerando menos como elemento de trabalho e mais como um meio de transporte. Em particular, se discutirá, por um lado, os seus valores ambíguos —progresso versus destruição— e a sua utilização como veículo não convencional de transporte de pessoas e produtos. E por outro lado, será discutida a ação estatal – punitiva e educativa – frente à deterioração dos caminhos rurais que tais práticas de mobilidade geraram. Por meio de um conjunto diversificado de fontes primárias textuais e visuais se demonstrará que a mecanização ocorrida no agro bonaerense transcendeu o âmbito meramente produtivo, motivando inconvenientes viários e desafiando o ordenamento normativo do trânsito.